• Reproductive and Developmental Biology
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• 제30권3호
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• pp.201-206
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• 2006

본 연구는 젖소에 있어서 Lipopolysaccharide의 처리가 착유우의 면역 반응에 미치는 영향을 구명하기 위하여 처리구 5두 및 대조구 3두를 공시하여 LPS 처리후 시간의 경과에 따른 PMNL의 비율, PMNL의 생존율 및 탐식 PMNL의 비율 등을 분석하였다. PMNL의 비율에 있어서, 처리전(0시간째)에는 대조구 및 처리구가 각각 41.0% 및 47.2%였으나 24시간째에는 41.7% 및 72.1%, 48시간째에는 41.0% 및 81.6%, 72시간째에는 44.3% 및 79.0%로 24시간째, 48시간째 및 72시간째에 공히 처리구가 대조구에 비하여 유의적(p<0.01)으로 높은 경향을 나타내었다. 대조구와 처리구의 PMNL의 생존율은 처리시간에 따른 차이가 없었다. 탐식 PMNL의 비율에 있어서, 처리전(0시간째) 및 처리 후 24시간째에는 대조구와 처리구간에 유의적인 차이를 나타내지 않았으나 48시간째에는 각각 1.1% 및 7.7%로 처리구가 대조구에 비하여 유의적(p<0.05)으로 높은 경향을 나타내다가 72시간째에는 유의적인 차이를 나타내지 않았다.

• 한국유기농업학회지
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• 제25권1호
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• pp.219-231
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• 2017

본 연구는 지방산 함량 및 배합사료와 조사료 비율을 기초로 한 유기농 사료 급여가 홀스타인 착유우에서 생산된 원유의 CLA 및 지방산 함량에 미치는 연구를 조사하기 위한 목적으로 실시하였다. 총 290두의 홀스타인 착유우를 산차 및 유량에 따라 3개 group으로 나누었다. 대조구는 C16:00, C18:2 그리고 SFA를 높게 설계하였고, 처리구 1은 C18:1, C18:2 그리고 UFA 함량을 높게 설계하였으며 처리구 2는 MUFA, C18:3 그리고 PUFA 함량을 높게 설계하였다. 결과를 요약하면 다음과 같다. 유기농 원유 내 C16:0 함량은 처리구 2에서 가장 높은 것으로 나타났다(p<0.05). 그 이유는 반추위 내 미생물의 de novo 생합성 때문인 것으로 판단된다. 처리구 2의 C18:0 함량은 7.92%로 대조구(11.39%)와 처리구 1(10.88%)보다 높았다(p<0.05). CLA 함량도 처리구 2가 처리구 1이나 대조구에 비하여 높은 것으로 나타났다(p<0.05). 원유 내에서 검출된 대부분의 CLA는 착유우 유선조직내의 ${\Delta}^9$-desaturase에 의하여 합성된 것으로 판단된다. n-3/n-6 비율도 처리구 2에서 가장 높은 것으로 나타났다(p<0.05). 본 연구 결과를 종합해 보면, 착유우에게 혼합 목건초 등을 급여하면 CLA, n-3 농도는 증가하며 C18:0 농도는 낮아지는 것으로 조사되었다. 본 연구는 유기농 인증된 조사료 및 농후사료를 이용하여 결과를 도출하였다. 그러나 유기농 사료를 사용할 경우에만 원유 내 CLA 및 n-3 농도는 증가한다고 볼 수 없다. 원유 내 고농도의 CLA 및 n-3 지방산 생산을 위해서는 반추위 미생물 및 유선세포의 지방 대사를 통한 CLA 생산 메커니즘에 대한 충분한 이해와 급여 사료 내 지방산 구성 등이 중요한 것으로 판단된다.

• Journal of Nutrition and Health
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• 제24권5호
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• pp.442-449
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• 1991

한국인 수유부 21명 의 모유에 함유된 철분, 아연, 및 구리의 함량을 경시적으로 초유부터 12주의 성숙유에 이르기까지 일정한 간격으로 세분하여 비교 검토하고, 생후 6~7주 된 14명의 모유영양아에 대한 1일 모유 및 미량원소 섭취량을 조사하였다. 모유에 함유된 이 세가지 미량원소들의 함량은 수유기간에 따라 유의적으로 감소 하였으며 모유의 평균 철분 함량은 초유에서 0.33$\mu\textrm{g}$/$m\ell$, 이행유에서 0.31$\mu\textrm{g}$/$m\ell$ 그리고 성숙유에서 0.2$\mu\textrm{g}$/$m\ell$이었고, 아연 농도는 각각 4.15$\mu\textrm{g}$/$m\ell$, 3.51$\mu\textrm{g}$/$m\ell$과 2.8$\mu\textrm{g}$/$m\ell$이었으며 초유와 이행유 그리고 성숙유의 평균 구리 농도는 각각 0.21$\mu\textrm{g}$/$m\ell$, 0.34$\mu\textrm{g}$/$m\ell$ 및 0.26$\mu\textrm{g}$/$m\ell$로 측정 되었다. 모유영양아의 평균 1일 모유 섭취량은 768g이었으며 모유를 통한 미량원소의 1일 평균 섭취량은 철분 0.19mg, 아연 2.10mg, 그리고 구리는 0.15mg 이었다. 앞으로 수유기간에 따른 모유내 미량원소 함량의 감소가 영아의 성장 발달 및 급식과 어떻게 관련되는가에 관한 연구와, 모유 영양아와 인공 영양아의 체내 미량윈소 영양 상태 비교가 이루 어져야 하겠으며 한국인 영아를 위한 영양 권장량은 성장이 양호한 모유 영양아의 영양소 섭취량을 기준하여 책정하는 것이 바람직 하다고 본다.

• 여성건강간호학회지
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• 제4권1호
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• pp.123-138
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• 1998

The purpose of this study was to describe perimenstrual symptom severity levels and perimenstrual distress patterns of women. The study performed the discriminant analysis in which included seven factors : age, pariety, social support, menstrual socialization(mother's symptom, sister's symptom, and menstrual effect), attitude of sex role and depression. The subjects were 283 women that they were not pregnant or lactating, had at least one period in past three months, would understand the purpose of study and willingly accepted the participation. The data analysis was done by pc-SAS program after data collection from Nov. 20, 1997 to Dec. 18, 1997. The descriptive analysis was done to explore general characteristics of the subjects and the stepwise discriminant analysis was done to verify factors in relation to perimenstrual symptom severity levels(severe vs mild menstrual symptom group) and perimenstrual distress patterns(spasmodic vs congestive menstrual symptom group). The instruments were selected for this study from Interpersonal Support Evaluation List(ISEL) by Cohen and Hoberman(1983), Center for Epidemic Studies Depression(CES-D) by Radloff(1977), and Sex Role Attitude Scale by Yunok Suh(1995), Mother's symptom and sister's symptom measurements by Woods, Mitchell & Lentz(1995), and menstrual effect by Brooks-Gun & Ruble(1980). The major findings of this study are as follows : 1. Of the 283 women, 93 women(32.9%) were assessed to severe perimenstrual symptom group and 190 women(67.1%) were assessed to mild perimenstrual symptom group. Results from the stepwise discriminant analysis showed three factors, such as depression, menstrual effect, and age, significantly related to perimenstrual symptom severity and they explained 20% of the total variance. The linear discriminant equation included three factors related to perimenstrual symptom groups was showed(Z=1.445 depression+0.174 menstrual effect-0.054 age). The cutting score(Z) was 2.809. We classified the severe perimenstrual symptom group by more than the cutting score 2.809 and the mild perimenstrual symptom by less or equal than the cutting score 2.809. The correctedness of posterior probability from discriminant equation was 72% as two perimenstrual symptom group classifications. 2. Of the 264 women, 139 women(52.7%) were assessed to spasmodic perimenstrual distress group and women(47.3%) were assessed to congestive perimenstrual distress group. Results from the stepwise discriminant analysis showed two factors, such as depression, age, significantly related to perimenstrual distress groups and they explained 8% of the total variance. The linear discriminant equation included two factors related to perimenstrual distress group was showed(Z=-0.084 age-0.776 depression). The cutting score(Z) was -3.759. We classified the spasmodic perimenstrual distress group by more than cutting score -3.759 and the congestive perimenstrual distress group by less or equal than cutting score -3.759. The correctedness of posterior probability from discriminant equation was 65% as two perimenstrual distress group classifications.

• 농업생명과학연구
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• 제51권6호
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• pp.137-144
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• 2017

A diverse of recommendation has been made for the structure and management of dairy cows, despite demanding research, the relationship between lactation number and various factors is yet to be established. The present study was aimed to investigate the covariance among lactation number, growth performance, calving interval, and milk production was considered to increase an efficiency of selection schemes and to manage more efficiently Holstein dairy cows that have been raised on small-scale family farms in Republic of Korea. For that purpose, the data were observed from 850 Holstein dairy cows, which a total of 3929 milking, since April 2016 - January 2017. We measured the body weight, height, age, calving interval, and milk production of the each dairy cow. Also, information about the date of lactation, calving interval, and milk production was recorded using an automatic milking system(AMS) with identification numbers. Milk production was calculated per udder quarter in the AMS. Our study results showed the increased average body weight(p>0.05) in 1, 2, 3, and $4^{th}$ lactating dairy cows and afterwards, we noticed the tendency on the average body weight(p<0.05) per lactation progressed. There was no significant difference noticed on height measurement of dairy cows. From the processing data of 850 Holstein dairy cows, the lactation number 1 and 7 had a greater calving interval with significantly lowered milk production, and the lactation number 2, 3, 4, 5, and 6 had significantly lowered the calving interval(p<0.05) with a greater milk production. From our study results, we evidenced that there is a significant relationship between the lactation number, growth performance, calving interval, and milk yield, and the maximum production of milk occurring in the $3^{rd}$ and $4^{th}$ lactation dairy cows. The achieved results from this study can be used by the small-scale farmers to encourage the structure and management of growth performance, calving interval, and milk yield in Holstein dairy cows in Korea.

• Journal of Animal Science and Technology
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• 제61권3호
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• pp.154-162
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• 2019

This experiment was conducted to evaluate the effect of dietary energy and crude protein (CP) levels on reproductive performance, litter performance, milk quality, and blood profiles in gestating sows. A total of 59 multiparous sows (Yorkshire ${\times}$ Landrace) with similar body weights (BW), backfat thickness (BF), and parity were assigned to one of six treatments with 9 or 10 sows per treatment using a $2{\times}3$ factorial arrangement and completely randomized design. The first factor was two levels of dietary metabolizable energy (ME) density (13.40 or 13.82 MJ/kg) and the second factor was three dietary protein levels based from 35 day in gestating phases (10.5%, 12%, and 13.5%). Backfat thickness change in lactating sows decreased linearly as CP level increased (p = 0.03). Increased energy level in the gestating sow diet tended to increase the total number of piglets born (p = 0.07), but piglet weight decreased (p = 0.02). Dietary CP level had a negative effect on colostrum quality. Casein, protein, total solid, and solids-not-fat concentrations decreased linearly and lactose level increased linearly as CP level in the gestating sow diet increased (casein%: p = 0.03; protein%: p = 0.04; lactose%: p = 0.06; total solids: p = 0.03; solid-not-fat: p = 0.03, respectively). However, improving ME by 0.42 MJ/kg had no significant effect on the chemical composition of sow colostrum. There were no significant differences in blood glucose concentration in gestating sows when sows were fed different levels of energy during gestation, but blood glucose increased at 21 day of lactation when energy increased by 0.42 MJ/kg (p = 0.04). Blood urea nitrogen concentration increased linearly when dietary CP levels increased at 110 day in gestation, 24-hours postpartum, and 21 days of lactation (linear, p < 0.05, p < 0.05, and p < 0.05, respectively), and it also increased when dietary energy increased at 110 days of gestation and 24-hours postpartum (p < 0.01, and p < 0.01, respectively). A gestating sow diet containing 13.82 MJ/kg ME and 10.5% CP can improve reproductive performance, litter performance, and colostrum quality.

• Animal Bioscience
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• 제34권1호
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• pp.74-84
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• 2021

Objective: Feed additives that modify rumen fermentation can be used to prevent metabolic disturbances such as acidosis and optimize beef cattle production. The study evaluated the effects of liquid and powdered forms of polyclonal antibody preparation (PAP) against Streptococcus bovis and Fusobacterium necrophorum on rumen fermentation parameters in ruminally cannulated non-lactating dairy cows that were adapted or unadapted to a high concentrate diet. Methods: A double 3×3 Latin square design was used with three PAP treatments (control, powdered, and liquid PAP) and two adaptation protocols (adapted, unadapted; applied to the square). Adapted animals were transitioned for 2 weeks from an all-forage to an 80% concentrate diet, while unadapted animals were switched abruptly. Results: Interactions between sampling time and adaptation were observed; 12 h after feeding, the adapted group had lower ruminal pH and greater total short chain fatty acid concentrations than the unadapted group, while the opposite was observed after 24 h. Acetate:propionate ratio, molar proportion of butyrate and ammonia nitrogen concentration were generally greater in adapted than unadapted cattle up to 36 h after feeding. Adaptation promoted 3.5 times the number of Entodinium protozoa but copy numbers of Streptococcus bovis and Fibrobacter succinogens genes in rumen fluid were not affected. However, neither liquid nor powdered forms of PAP altered rumen acidosis variables in adapted or unadapted animals. Conclusion: Adaptation of cattle to highly fermentable carbohydrate diets promoted a more stable ruminal environment, but PAP was not effective in this study in which no animal experienced acute or sub-acute rumen acidosis.

• 대한지역사회영양학회지
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• 제26권1호
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• pp.48-55
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• 2021

Objectives: This study was conducted to estimate the dietary iodine intake of Koreans by a Total Diet Study (TDS) which provides 'closer-to-real' estimates of exposure to hazardous materials and nutrients through an analysis of table-ready (cooked) samples of foods. Methods: Dietary intake data from 2013-2017 Korea National Health and Nutrition Examination Survey (KNHANES) was used to select representative foods (RFs) for iodine analysis. A total of 115 RFs were selected and 158 'RF × cooking method-combination' pairs were derived by pairing each RF to corresponding cooking method(s) used more frequently. RFs were collected from 9 mega-markets in 9 metropolitan cities nationwide and mixed into composites prior to cooking preparation to a 'table ready' state for iodine analysis by inductively coupled plasma mass spectrometry. Iodine intake of Koreans was estimated based on the food intake data of the 2016-2018 KNHANES. Results: High iodine content was detected in seaweeds such as sea mustard and kelp. The mean iodine intake/capita/day was 418.4 ㎍ and the median value was 129.0 ㎍. Seaweeds contributed to 77.4% of the total iodine intake and the contribution by food item was as follows: sea mustard (44.0%), kelp (20.4%), laver (13.1%), milk (3.9%), egg (3.5%). Compared to the Dietary Reference Intakes for Koreans 2020, the proportion of people with iodine intake exceeding the tolerable upper intake level or below the estimated average requirement was high in the physiologically vulnerable groups (infants, children, pregnant women, and lactating women). Conclusions: The results, drawn from a TDS, are regarded closer to real estimates for iodine intake of Koreans compared with values in existing literature, which were based on a very limited variety of foods. On the other hand, it seems necessary to seek out solutions for the problematic iodine intake among physiologically vulnerable groups through in-depth analyses on food intake data collected with significant scale & quality.

• 한국산학기술학회논문지
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• 제21권12호
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• pp.440-448
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• 2020

본 연구는 제7기 3차년도(2018) 국민건강영양조사 통계 자료 중 건강 설문 조사 결과를 토대로 인구사회학적 특성 및 건강관련 특성이 성인 여성의 모유수유에 미치는 영향요인을 파악하고자 시행되었다. 자료는 가중치를 적용하여 SPSS Win 22.0 프로그램의 복합표본 분석방법을 이용하여 분석하였다. 성인 여성의 모유수유에 영향을 미치는 요인을 알아보기 위하여 복합표본 다중 로지스틱 회귀분석을 한 결과, 연령, 교육 수준, 흡연이 모유수유에 영향을 미치는 것으로 나타났다. 30-39세 연령군인 경우 19-29세 연령군인 경우보다 3.06배, 초등학교 이하의 학력인 경우가 대학 이상의 학력인 경우보다 4.70배, 비흡연자가 흡연자에 비해 2.51배 모유수유율이 높았다. 따라서 30세 미만의 연령의 경우 모유 수유에 대한 교육을 더욱 강화하고 40세 이상의 연령의 경우 모유생산량을 높이고 돌봄 제공서비스를 제공해야 한다. 또한 학력이 높은 경우에는 직장생활 등의 제한된 환경에서의 효율적인 모유수유 방법을 익히고 수유부를 배려하는 사회적 분위기 조성이 필요할 것이다. 뿐만 아니라 여성 흡연자의 금연프로그램에는 임신 및 수유와 관련된 교육이 포함되어야 할 것이다.

• Journal of Nutrition and Health
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• 제54권6호
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• pp.584-593
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• 2021

2020 한국인 영양소 섭취기준에서 탄수화물은 만성질환 예방을 위한 섭취기준으로 총 에너지 섭취량에 대한 탄수화물로부터의 에너지 섭취비율인 에너지적정비율을 1세 이후 모든 연령에서 55-65%로설정하였다. 그리고 2020년에 처음으로 탄수화물의 평균필요량과 권장섭취량을 설정하였다. 인체의 탄수화물 필요량은 뇌에서 하루에 소비되는 포도당량과 케톤체 생성이 나타나지 않는 양을 기준으로 1세 이후 모든 연령에서 1일 100 g을 설정하고, 권장섭취량은 15% 변이계수를 사용하여 두 배의 변이계수를 더한 130 g/일로 설정하였다. 탄수화물의 평균필요량은 최소필요량의 개념이지 에너지원으로서 적절한 섭취량에 대한 개념은 아니므로 탄수화물의 영양소섭취상태를 평가할 경우에는 평균필요량이나 권장섭취량에 비교하는 것은 적절하지 않고, 에너지적정비율을 활용하여 탄수화물로부터의 에너지 섭취비율의 적절성을 평가하는 것이 적절하다. 탄수화물은 에너지 적정비율범위에서 섭취할 때 만성질환의 위험을 낮출 수 있으며, 이를 탄수화물의 양뿐만 아니라 급원도 고려하는 식생활에 활용해야 할 것이다.