Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of Intra-Uterine Lipopolysaccharides Injection on Immunological Response of Uterus in Lactating Holsteins

젖소에 있어서 Lipopolysaccharide의 처리가 면역 반응에 미치는 영향

  • Baek Kwang-Soo (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Park, Soo-Bong (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Park, Seong-Jai (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Lee, Wang-Sik (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Ki, m, Hyeon-Shup (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Jeong, Gyeong-Yong (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Ki, Kwang-Seok (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Jeon Byeong-Soon (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Ah, Byeong-Seog (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Lee, Hyeon-Jun (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Khan M. Ajmal (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Ki, m, Tae-Il (Dairy Cattle Research Division, National Livestock Research Institute, RDA)
  • 백광수 (농촌진흥청 축산연구소 낙농과) ;
  • 박수봉 (농촌진흥청 축산연구소 낙농과) ;
  • 박성재 (농촌진흥청 축산연구소 낙농과) ;
  • 이왕식 (농촌진흥청 축산연구소 낙농과) ;
  • 김현섭 (농촌진흥청 축산연구소 낙농과) ;
  • 정경용 (농촌진흥청 축산연구소 낙농과) ;
  • 기광석 (농촌진흥청 축산연구소 낙농과) ;
  • 전병순 (농촌진흥청 축산연구소 낙농과) ;
  • 안병석 (농촌진흥청 축산연구소 낙농과) ;
  • 이현준 (농촌진흥청 축산연구소 낙농과) ;
  • ;
  • 김태일 (농촌진흥청 축산연구소 낙농과)
  • Published : 2006.09.30
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Abstract

This study was carried cut to determine the immunological response of uterus-induced by Lipopolysaccharides (LPS) in Holstein cows. The LPS isolated from Bacteroids helcogenes and Fusobacterium varium was injected at the rate of 100 ${\mu}g$ with 30 ml of phospahte buffer saline(PBS) in each cow(n=5). Three cows were acted as control. There was no difference in total polymorphonuclear leukocytes(PMNL) concentration in uterine fluid between control and LPS groups at 24, 48 and 72 hrs after LPS treatment. There was significant difference in rate of PMNL between control and LPS groups at 24(41.7% vs 72.1%), 48(41.0% vs 81.6%) and 72 hrs(44.3% vs 79.0%) after LPS treatment. There was no difference in PMNL viability between control and LPS groups at 24, 48 and 72 hrs after LPS treatment. There was significant difference in rate of phagocytic PMNL between control and LPS groups at 48 hr after LPS treatment(1.1% vs 7.7%).

본 연구는 젖소에 있어서 Lipopolysaccharide의 처리가 착유우의 면역 반응에 미치는 영향을 구명하기 위하여 처리구 5두 및 대조구 3두를 공시하여 LPS 처리후 시간의 경과에 따른 PMNL의 비율, PMNL의 생존율 및 탐식 PMNL의 비율 등을 분석하였다. PMNL의 비율에 있어서, 처리전(0시간째)에는 대조구 및 처리구가 각각 41.0% 및 47.2%였으나 24시간째에는 41.7% 및 72.1%, 48시간째에는 41.0% 및 81.6%, 72시간째에는 44.3% 및 79.0%로 24시간째, 48시간째 및 72시간째에 공히 처리구가 대조구에 비하여 유의적(p<0.01)으로 높은 경향을 나타내었다. 대조구와 처리구의 PMNL의 생존율은 처리시간에 따른 차이가 없었다. 탐식 PMNL의 비율에 있어서, 처리전(0시간째) 및 처리 후 24시간째에는 대조구와 처리구간에 유의적인 차이를 나타내지 않았으나 48시간째에는 각각 1.1% 및 7.7%로 처리구가 대조구에 비하여 유의적(p<0.05)으로 높은 경향을 나타내다가 72시간째에는 유의적인 차이를 나타내지 않았다.

Keywords

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