• The Korean Journal of Mycology
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• v.16 no.4
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• pp.242-246
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• 1988

To determine contents of inorganic elements of Laccaria, the carpophores of Laccaria laccata(Fr.) Berk. et Br., Laccaria amethystina(Fr.) Berk. et Br., Laccaria tortilis(S.P. Gray) Cooke and Laccaria vinaceoavellanea Hongo were incinerated and analyzed by atomic absorption spectrophotometer. Total contents of determined inorganic elements(except Cd) were highst in Laccaria tortilis than the other three mushrooms, and Laccaria vinaceoavellanea, Laccaria laccata and Laccaria amethystina in that order. The four species mushrooms contained potassium, iron, zinc, sodium, copper, manganese, and calsium in that order, and the content of potassium was the highst than the other inorganic components. The content of cadmium was Laccaria tortilis, Laccaria laccata, Laccaria vinaceoavellanea and Laccaria amethystina in that order.

• Mycobiology
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• v.48 no.4
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• pp.288-295
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• 2020

Species of Laccaria (Hydnangiaceae, Agaricales, and Basidiomycota) are well-known ectomycorrhizal symbionts of a broad range of hosts. Laccaria species are characterized by brown, orange, or purple colored basidiocarps, and globose or oblong, echinulate and multinucleate basidiospores. While some Laccaria species are easily identified at the species level using only the morphological characteristics, others are hard to distinguish at the species level due to small differences in morphology. Heretofore, ten Laccaria species have been reported in Korea. While studying the fungal diversity in the National Parks of Korea, two new Laccaria species were discovered. Species identification was done based on molecular analyses (ITS, 28S rDNA, rpb2, and tef1), then were confirmed by their corresponding morphologies. The two newly discovered Laccaria species are proposed here as Laccaria macrobasidia and Laccaria griseolilacina. The unique morphological characters of L. macrobasidia that distinguish it from its closely related species are orange-brown colored basidiocarp, long basidia and the absence of cheilocystidia. L. griseolilacina is characterized by a light grayish lavender-colored pileus and the absence of cheilocystidia. Two new species are described and illustrated in the present paper.

• Mycobiology
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• v.49 no.5
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• pp.461-468
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• 2021

The genus Laccaria (Hydnangiaceae, Agaricales) plays an important role in forest ecosystems as an ectomycorrhizal fungus, contributing to nutrient cycles through symbiosis with many types of trees. Though understanding Laccaria diversity and distribution patterns, as well as its association with host plants, is fundamental to constructing a balanced plant diversity and conducting effective forest management, previous studies have not been effective in accurately investigating, as they relied heavily on specimen collection alone. To investigate the true diversity and distribution pattern of Laccaria species and determine their host types, we used four different approaches: specimen-based analysis, open database search (ODS), NGS analysis, and species-specific PCR (SSP). As a result, 14 Laccaria species have been confirmed in Korea. Results regarding the species distribution pattern were different between specimen-based analysis and SSP. However, when both were integrated, the exact distribution pattern of each Laccaria species was determined. In addition, the SSP revealed that many Laccaria species have a wide range of host types. This study shows that using these four different approaches is useful in determining the diversity, distribution, and host of ECM fungi. Furthermore, results obtained for Laccaria will serve as a baseline to help understand the role of ECM fungi in forest management in response to climate change.

• Archives of Pharmacal Research
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• v.5 no.1
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• pp.17-19
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• 1982

To develop potent anitumor polysac-charides from higher hungi of Korea the alcohol precipitated polysaccharides, which were obtained from hot water extracts of shake-cultured mycelia of eight species of basidiomycetes, were subjected to antitumor screening tests, using sarcoma 180 as a tumor cell. When administered i. p, at the dose level of 20mg/kg/day for ten days into the mice which were implanted with $1{times}10^{8}$ cells of sarcoma 180 twenty-four hour before the start of polysaccharide injection, the polysaccharides of Plutcus cervinus ISA-Pc-1004, Pleurotus pulmonarius, Hydnum repandum ISA-Hr-1006, and Laccaria laccata ISA-LI-1008 respectively showed inhibition ratios of 62.0%, 51.7%, 64.8%, and 57.8%. They were selected for further study, while those of Pancillus serotina ISA-Ps-1012, Laccaria amethystina ISA-La 1001, Pholiota adiposa ISA-Pa-1010, and Lepista spISA-Ls-1007 respectively showed inhibition ratios of -9.3%, 25.3%, 27.3% and 33.0%.

• The Korean Journal of Mycology
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• v.28 no.2
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• pp.123-125
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• 2000

To investigate the hemolytic toxicities of the Korean basidiomycetes, the cold-water extracts of 22 wild mushrooms were tested for their hemolytic activities on the erythrocytes of a mouse and a rat. As a result, three species including Collybia confluence, Laccaria vinaceoavellanea and Lenzites betulina showed strong hemolytic activities. Of these, L. vinaceoavellanea retained more than 80% of its hemolytic activity even after it was boiled for 30 minutes, while the other two were inactivited in five minutes.

• The Korean Journal of Mycology
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• v.11 no.1
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• pp.9-13
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• 1983

Ectomycorrhizal fungi in Pinus $rigida{\times}taeda$ and poplar stands (Populus $alba{\times}glandulosa$ and others) were collected in 1981 and 1982. A total of 39 mushrooms representing 15 genera were identified from the pine stand, and the major genera were Amanita, Lactarius, Russula, Laccaria, and Boletus. From the poplar stands 16 mushrooms representing 8 genera were identified and 13 of them were the same found in the pine stand. Amanita, Russula, Laccaria, and Leccinum are the major genera in the poplar stands. A single species of Boletus was found in large number in pine and poplar stands. Lactarius was not observed in the poplar stands, while Leccinum was not found in the pine stand. Cantharellus lutescens which has not been reported in Korea was collected in the pine stand.

• Korean Journal of Plant Resources
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• v.9 no.1
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• pp.31-39
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• 1996

Many higher fungi were collected at MI.Moak Provincial Park (Chonlabuk-Do), Mt.Pangtae(Kangwon-Do) and Byunsan Pennisula National Park from April. 1995 to October, 1995. These higher fungi were identified.According to the results, these species were newly to Korea: Laccaria ohiellsis, Mycef1a clavularis. M.acicula. Marasmius ramealis. M.calopus, Strobilurus esculefltus, Agrocybe sphaleromorpha and Corlinarius fasciatus.

• The Korean Journal of Mycology
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• v.15 no.1
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• pp.48-69
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• 1987

To understand host ranges and host specificity of ectomycorrhizal fungi and fungus specificity of host tree species, higher fungi from pure forest stands of following twelve tree species were collected for six years from 1981 to 1986: Pinus densiflora, P. rigida, P. koraiensis, Picea koraiensis, Larix leptolepis, Abies holophylla, Populus $alba{\times}glandulosa$, Castanea crenata, Quercus aliena, Q. acutissima, Q. mongolica, and Betula platyphylla. A total of 196 ectomycorrhizal species with 8 varieties in 48 genera were identified. Thirty-one species with one variety belonged to Russula, 22 species with 5 varieties to Amanita, 18 species with one variety to Lactarius. Fungi belonging to above three gepera, Laccaria, and Cantharellus had a relatively wide host range. Laccaria laccata was collected under all the 12 tree species, Amanita vaginata group under 11 tree species, Laccaria amethystina and Russula foetens under 10 tree species, Lactarius gerardii and Russula sororia under 9 tree species, and Amanita agglutinata, Cantharellus cibarius, Russula bella, and R. virescens under 8 tree species. Above 10 fungal species may be classified to have a wide host range. Following fungi were collected under 4 to 5 tree species of both conifers and broad-leaved trees: Amanita citrina, Boletus bicolor, B. erythropus, Lactarius piperatus, L. subzonar­ius, and Russula pseudodelica. Above fungi may be classified to have an intermediate host range. Chroogomphus, Gomphidius, Rhizopogon, and Suillus were collected only under Pinaceae and may be classified to have a narrow host range. Particularly Suillus grevillei was collected only under Larix leptolepis, and appeared to have high host specificity. A total of 83 ectomycorrhizal species were collected under Abies holophylla, 66 species under Pinus densiflora, 50 species under Pinus rigida, 49 species under Pinus koraiensis, 46 species under Quercus aliena, and 23 fungal species under Larix leptolepis. Particularly, Larix leptolepis was associated with different fungal flora, with fewer species of Amanita, Lactarius and Russula, and more species of Laccaria. Most host tree species surveyerl in the present study appeared to have low fungus specificity for ectomycorrhizal formation.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.474-479
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• 2010

In this paper, we analyzed the microsatellites in the transcript sequences of the whole Laccaria bicolor genome. Our results revealed that, apart from the triplet repeats, length diversification and richness of the detected microsatellites positively correlated with their repeat motif lengths, which were distinct from the variation trends observed for the transcriptional microsatellites in the genome of higher plants. We also compared the microsatellites detected in the genic regions and in the nongenic regions of the L. bicolor genome. Subsequently, SSR primers were designed for the transcriptional microsatellites in the L. bicolor genome. These SSR primers provide desirable genetic resources to the ectomycorrhizae community, and this study provides deep insight into the characteristics of the micro satellite sequences in the L. bicolor genome.

• The Korean Journal of Mycology
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• v.12 no.1
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• pp.35-43
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• 1984

To produce and characterize antineoplastic constituents in the submerged cultured­mycelia of Laccaria laccata, the mycelia were extracted with distilled water. Purification of the extract was carried out by acetone precipitation, by ion exchange chromatography using DEAE­Sephadex A-50, CM-Sephadex C-25 resins, and by gel filtration chromatography on Sephadex G-200. Each fraction obtained during the purification was examined for antineoplastic activity against sarcoma 180 in ICR mice. As the purification proceeded, the antineoplastic activity was markedly increased. The highly purified Fraction E showed 75% tumor inhibition ratio at a dose of 10mg/kg/day and contained 81% polysaccharide and 4% protein. The antitumor component of Fraction E stimulated an accumulation of peritoneal exudate cells including peritoneal macrophages, and is named laccaran.