• Asian Pacific Journal of Cancer Prevention
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• 제16권2호
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• pp.719-722
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• 2015

Objective: To evaluate the values of 4 tumor markers in serum and ascites and their ascites/serum ratios in the identification and diagnosis of benign and malignant ascites. Materials and Methods: A total of 76 patients were selected as subjects and divided into malignant ascites group (45 cases) and benign ascites group (31 cases). Samples of ascites and serum of all hospitalized patients were collected before treatment. The levels of carcinoembryonic antigen (CEA), alpha fetoprotein (AFP), cancer antigen 125 (CA125) and carbohydrate antigen 19-9 (CA19-9) were detected by chemiluminescence (CLIA). Results: CEA, AFP and CA19-9 in both serum and ascites as well as CA125 in ascites were evidently higher in the malignant ascites group than in the benign ascites group (P<0.01). Malignant ascites was associated with elevated ascites/serum ratios for AFP and CA125 (P<0.01). The areas under receiver operating characteristic (AUROCs) of CEA and CA125 in ascites and the ratios of ascites/serum of AFP, CEA, CA125 and CA19-9 were all >0.7, suggesting certain values, while those of ascites CA19-9 and serum CEA were 0.697 and 0.629 respectively, indicating low accuracy in the identification and diagnosis of benign and malignant ascites. However, the AUROCs of the remaining indexes were <0.5, with no value for identification and diagnosis. Compared with single index, the sensitivity of combined detection increased significantly (P<0.05), in which the combined detection of CEA, CA19-9 and CA125 in ascites as well as the ratio of ascites/serum of CEA, CA19-9, CA125 and AFP had the highest sensitivity (98.4%) but with relevantly low specificity. Both sensitivity and specificity of combined detection should be comprehensively considered so as to choose the most appropriate index. Conclusions: Compared with single index, combined detection of tumor markers in serum and ascites can significantly improve the diagnostic sensitivity and specificity.

• Asian Pacific Journal of Cancer Prevention
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• 제13권4호
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• pp.1693-1697
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• 2012

Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.

• 지구물리
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• 제3권1호
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• pp.1-12
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• 2000

경상분지내 밀양소분지의 건천리 지역에 분포하는 백악기 하양층군의 송내동층, 채약산화산암, 건천리층, 그러고 유천층군 하부인 주사산안산암을 대상으로 지층생성 당시의 자북의 위치와 퇴적시기를 결정하기 위하여 12개 노두에서 106개의 정향시료 채취하여 고자기 연구를 수행하였다. 연구결과 주 자성광물인 적철석과 자철석에 의하여 기록된 이들 지층들의 평균 특성잔류자기 방향은 역전검사 그리고 통계학적으로 99%의 신뢰도를 가지며 습곡검사 통과하였다. 단계적 습곡검사에서는 군집지수 k값의 변화가 90%의 지층경사보정 단계에서 최대를 보여서 이들 지층들의 특성잔류 자기는 지층경사나 습곡작용 이전 즉, 퇴적 동시기이거나 그 직후에 획득된 1차 잔류자기임을 지시한다. 이들의 평균 방향과 고자기극의 위치는 각자 $D=22.9^{\circ},\;I=59.1^{\circ}\;(k=410,\;{\alpha}_{95}=3.0^{\circ},\;N=7sites)$와 $199.6^{\circ}E,\;71.6^{\circ}N\;(K=206.9,\;A_{95}=4.2^{\circ})$이다. 이는 중국대륙 및 경상분지내 다른 동시대 지층들에서 구한 방향들과 오차한계 내에서 동일한 것으로서 연구지역과 이들 지역간에 상대적 변위나 수평회전운동이 거의 없었음을 지시한다. 자기층서학적 견지에서 볼 때, 연구지층들은 백악기 상부 알비안(upper Albian)에서 하부 캄파니안(lower Campanian)의 역자극기까지의 것으로 대비된다. 한편, 채약산화산암과 주사산안산암의 응회암과 및 각력암에서는 분산된 특성잔류자기 방향이 나타났는데 이는 암석들이 잔류자기를 획득한 후에 화산함몰 구조운동에 의하여 재동(reworked)되었음을 의미한다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권13호
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• pp.5155-5160
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• 2014

Aim: To investigate the efficacy and safety of lobaplatin-transcatheter arterial chemoembolization (TACE) combined with radioactive $^{125}I$ seed implantation in treatment of primary hepatocellular carcinoma (HCC). Methods: 75 patients with primary HCC were enrolled in the study, among them 43 receiving lobaplatin-TACE (TACE group) and 32 lobaplatin-TACE combined with $^{125}I$ seed implantation (TACE+$^{125}I$ group). After treatment, the local remission rates and postoperative complications of two groups were compared using the Pearson Chi-square test. Overall survival in the two groups was calculated using Kaplan-Meier survival curves and the differences were tested using Log-rank test. Results: There were 7 cases of complete response (CR), 13 of partial response (PR), 6 of stable disease (SD) and 17 of progressive disease (PD) in the TACE group, with 13 cases of CR, 9 of PR, 5 of SD and 5 of PD in the TACE+$^{125}I$ group. The disease control rates of TACE and TACE+$^{125}I$ group were 60.5% (26/43) and 84.4% (27/32), respectively, with a significant difference between them (P < 0.05). The survival rates at 6, 12 and 18 months in the TACE group were 100.0%, 81.8% and 50.0%, respectively, and those in TACE+$^{125}I$ group were 100.0%, 93.8% and 65.6%. The mean survival times in the TACE and TACE+$^{125}I$ groups were 19.5 and 22.9 months, respectively. There was a significant difference in the overall survival rate between two groups (P < 0.05). No serious complications were encountered in either group. Conclusion: Lobaplatin-TACE combined with $^{125}I$ seed implantation is favorable and safe for treatment of primary HCC.

• Asian Pacific Journal of Cancer Prevention
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• 제13권3호
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• pp.785-789
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• 2012

Background and Purpose: Cisplatin is the most common chemotherapeutic agent for loco-regionally advanced nasopharyngeal carcinoma (NPC); however, toxicity is a limiting factor for some patients. We retrospectively compared the efficacy and toxicity of weekly docetaxel-based and cisplatin-based concurrent chemoradiotherapy in loco-regionally advanced NPC. Methods and Materials: Eighty-four patients with Stage III and IVA-B NPCs, treated between 2007 and 2008, were retrospectively analyzed. Thirty received weekly docetaxel-based concurrent chemotherapy, and 43 were given weekly cisplatin-based concurrent chemotherapy. Radiotherapy was administered using a conventional technique (seven weeks, 2.0 Gy per fraction, total dose 70-74 Gy) with 6-8 Gy boosts for some patients with locally advanced disease. Results: Median follow-up time was 42.3 months (range, 8.6-50.8 months). There were no significant differences in the 3-year loco-regional failure-free survival (85.6% vs. 92.3%; p=0.264), distant failure-free survival (87.0% vs. 92.5%; p=0.171), progression-free survival (85.7% vs. 88.4%; p=0.411) or overall survival (86.5% vs. 92.5%, p=0.298) of patients treated concurrently with docetaxel or cisplatin. Severe toxicity was not common in either group. Conclusions: Weekly docetaxel-based concurrent chemoradiotherapy is potentially effective and has a tolerable toxicity; however, further investigations are required to determine if docetaxel is superior to cisplatin for advanced stage NPC.

• 한국약용작물학회지
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• 제7권4호
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• pp.245-250
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• 1999

현탁배양에 의하여 fusaric acid 저항성인 세포주를 선발하고, 선발된 세포주들의 fusaric acid 저항성, 저항성의 안정성 및 식물체 재분화 능력을 조사하였다. 선발된 20개의 세포주의 저항성 정도는 세포주에 따라 상이하였으나, 일반적으로 fusaric acid 감수성인 세포주보다 fusaric acid가 첨가된 배지에서 높은 캘러스 생장을 보여 높은 저항성을 나타냈으며 RF-9, RF-11, RF-15는 $100\;{\mu}M$에서도 70% 이상의 높은 캘러스 생장을 나타냈다. Fusaric acid가 첨가되지 않은 배지에서 5주 동안 계대 배양한 후, fusaric acid 안정성을 조사한 결과, $10\;{\mu}M$에서는 모두60% 이상의 생장율을 나타냈으며, $100\;{\mu}M$에서는 약 30%에서 80%까지 생장이 억제됨을 보여 농도가 높아질수록 캘러스 생장 억제 정도가 증가함을 알수 있었다. Fusaric acid 저항성 세포주들의 식물체 분화능력은 세포주에 따라 상이하였으며, $50\;{\mu}M$ fusaric acid 농도에서도 13개의 세포주들이 1개 이상의 줄기가 분화되었고 두개의 세포주는 분화가 되지 않았다.

• 한국산림과학회지
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• 제26권1호
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• pp.13-18
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• 1975

본시험(本試驗)은 죽간재적(竹稈材積)에 영향(影響)하는 형질(形質)을 정확(正確)하게 파악(把握)하기 위(爲)하여 계획(計劃)되었다. 그리고 왕대의 수개형질상호간(數個形質相互間)의 표현형상관(表現型相關), 유전상관(遺傳相關) 및 환경상관(環境相關)을 구명(究明)하고져 공분산분석(共分散分析) 되었으며 Dewey and Lu의 편회귀분석법(偏回歸分析法)으로 각형질(各形質)이 죽간재적(竹稈材積)에 미치는 효과(効果) 즉(即) 경로계수(經路係數)를 산출(算出)하였다. 조사형질(調査形質)은 흉고직경(胸高直徑), 죽간고(竹稈高), 지상고(枝上高), 지하경(地下莖), 절수(節數), 근원직경(根元直徑) 및 죽간재적(竹稈材積)이며 각(各) 형질(形質)의 평균치(平均値), 표준오차(標準誤差), 분산(分散) 및 표준편차(標準偏差)는 제(第)1표(表)와 같고 7개형간(個形間)의 표현형(表現型), 유전(遺傳) 및 환경상관(環境相關)을 분산분석(分散分析)한 결과(結果)는 제(第)2표(表)와 같이 계통간(系統間)에는 고도(高度)의 유의성(有意性)이 인정(認定)되었으나 몇몇 반복간(反覆間)에는 유의성(有意性)이 인정(認定)되지 않았다. 각(各) 형질(形質)의 유전력(遺傳力)을 추정(推定)한바 흉고직경(胸高直徑)과 근원직경(根元直徑)은 유전력(遺傳力)이 높고 기타형질(其他形質)의 유전력(遺傳力)은 제(第)4표(表)에서와 같이 보다 낮으며 환경상관(環境相關)은 표현형상관(表現型相關), 유전상관(遺傳相關)보다 전체적(全體的)으로 낮다. 그리고 죽간재적(竹稈材積)에 대(對)하여 근원직경(根元直徑)과 흉고직경(胸高直徑)은 높은 상관관계(相關關係)를 나타내었다. 형질별(形質別) 상관관계(相關關係), 경로계수(經路係數) 및 죽간재적(竹稈材積)에 미치는 직접(直接), 간접효과(間接効果)는 도표(圖表) 1과 제(第)5표(表)에서 볼 수 있으며 결과적(結果的)으로 본시험(本試驗)에서는 죽간재적수량(竹稈材積收量)은 근원직경(根元直徑)과 흉고직경(胸高直徑)에 의(依)하여 좌우(左右)될 수 있다는 것을 알았다.

• Korean Journal of Acupuncture
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• 제21권2호
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• pp.147-159
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• 2004

Objective : This study was designed to estimate the effects of Rainbow Power therapy on neck pain patients by using Visual Analogue Scale(VAS) and pressure algometer. Methods : Rainbow Power therapy group consisted of 18 patients and acupuncture therapy group consisted of 7 patients. The degree of improvement of neck pain was evaluated by VAS and pain pressure threshold(at the points of Jianjing GB21 and Jianwaishu SI14) before treatment, before 3rd treatment and before 5th treatment. Rainbow Power therapy was performed at the points of Jianjing GB21, Zhongfu LU1, Yangxi LI5, Ganshu BL18, Weishu BL21 and Shenshu, BL23. Each points were stimulated with RP-UM103(Rainbow Power therapy instrument) for 20 seconds. The points of acupuncture therapy were Jeonggeun, Jeongjong, Sangbaekn and Hegu LI4 and acupuncture was maintained for 15 minutes. After above therapy dry cupping was performed at the points of Jianjing GB21, Jianwaishu SI14, Gaohuang BL43, Tianzong, SI11 and Bingfeng SI12 for 5 minutes. Results : There was no significant difference between the two groups in VAS, pain threshold of Jianjing GB21 and Jianwaishu SI14 following treatment. In the two groups VAS was significantly decreased. In Rainbow Power therapy group the pain threshold of the two points was significantly increased. But there was no significant increase in acupuncture therapy groups about the pain threshold of Jianwaishu SI14. Conclusions : The effectiveness of Rainbow Power therapy on neck pain was shown through VAS and pressure algometer. These imply that Rainbow Power therapy may be useful for neck pain. Further study is needed about Rainbow Power therapy.

• Journal of Microbiology
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• 제45권1호
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• pp.21-28
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• 2007

In order to search for specific genotypes related to this unique phenotype, we used whole genomic DNA microarray to characterize the genomic diversity of Helicobacter pylori (H. pylori) strains isolated from clinical patients in China. The open reading frame (ORF) fragments on our microarray were generated by PCR using gene-specific primers. Genomic DNA of H. pylori 26695 and J99 were used as templates. Thirty-four H. pylori isolates were obtained from patients in Shanghai. Results were judged based on In(x) transformed and normalized Cy3/Cy5 ratios. Our microarray included 1882 DNA fragments corresponding to 1636 ORFs of both sequenced H. pylori strains. Cluster analysis, revealed two diverse regions in the H. pylori genome that were not present in other isolates. Among the 1636 genes, 1091 (66.7%) were common to all H. pylori strains, representing the functional core of the genome. Most of the genes found in the H. pylori functional core were responsible for metabolism, cellular processes, transcription and biosynthesis of amino acids, functions that are essential to H. pylori's growth and colonization in its host. In contrast, 522 (31.9%) genes were strain-specific genes that were missing from at least one strain of H. pylori. Strain-specific genes primarily included restriction modification system components, transposase genes, hypothetical proteins and outer membrane proteins. These strain-specific genes may aid the bacteria under specific circumstances during their long-term infection in genetically diverse hosts. Our results suggest 34 H. pylori clinical strains have extensive genomic diversity. Core genes and strain-specific genes both play essential roles in H. pylori propagation and pathogenesis. Our microarray experiment may help select relatively significant genes for further research on the pathogenicity of H. pylori and development of a vaccine for H. pylori.

• Asian-Australasian Journal of Animal Sciences
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• 제33권8호
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• pp.1265-1272
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• 2020

Objective: To evaluate the effect of feeding acidified milk on the growth and fecal microbial diversity of dairy calves. Methods: Twenty healthy 3-day-old female Holstein calves with similar body weights were selected and randomly divided into two groups. One group was fed pasteurized milk (PM, Control), while the other was fed acidified milk (AM) ad libitum until weaned (day 60). The experiment lasted until day 180. Results: There was no difference in the nutritional components between PM and AM. The numbers of Escherichia coli and total bacteria in AM were lower than in PM. At 31 to 40 and 41 to 50 days of age, the milk intake of calves fed AM was higher than that of calves fed PM (p<0.05), and the solid feed intake of calves fed AM was higher than that of calves fed PM at 61 to 90 days (p<0.05). The average daily gain of calves fed AM was also higher than that of calves fed PM at 31 to 60, 61 to 180, and 7 to 180 days (p<0.05). The calves fed AM tended to have a lower diarrhea rate than those fed PM (p = 0.059). Bacteroides had the highest abundance in the feces of calves fed AM on day 50, while Ruminococcaceae_UCG_005 had the highest abundance in the feces of calves fed AM on day 90 and calves fed PM on days 50 and 90. At the taxonomic level, the linear discriminant analysis scores of 27 microorganisms in the feces of calves fed AM and PM on days 50 and 90 were higher than 4.0. Conclusion: Feeding AM increased calf average daily gain and affected fecal bacterial diversity.