• Animal Bioscience
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• 제35권7호
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• pp.975-988
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• 2022

Objective: In this study, we aimed to identify long non-coding RNAs (lncRNAs) that play important roles in starvation stress, analyze their functions, and discover potential molecular targets to alleviate starvation stress to provide a theoretical reference for subsequent in-depth research. Methods: We generated a piglet starvation stress animal model. Nine Yorkshire weaned piglets were randomly divided into a long-term starvation stress group (starved for 72 h), short-term starvation stress group (starved for 48 h), and the control group. LncRNA libraries were constructed using high-throughput sequencing of piglet ileums. Results: We obtained 11,792 lncRNAs, among which, 2,500 lncRNAs were novel. In total, 509 differentially expressed (DE)lncRNAs were identified in this study. Target genes of DElncRNAs were predicted via cis and trans interactions, and functional and pathway analyses were performed. Gene ontology functions and Kyoto encyclopedia of genes and genomes analysis revealed that lncRNA-targeted genes mainly participated in metabolic pathways, cellular processes, immune system processes, digestive systems, and transport activities. To reveal the mechanism underlying starvation stress, the interaction network between lncRNAs and their targets was constructed based on 26 DElncRNAs and 72 DEmRNAs. We performed an interaction network analysis of 121 DElncRNA-DEmRNA pairs with a Pearson correlation coefficient greater than 0.99. Conclusion: We found that MSTRG.19894.13, MSTRG.16726.3, and MSTRG.12176.1 might play important roles in starvation stress. This study not only generated a library of enriched lncRNAs in piglets, but its outcomes also provide a strong foundation to screen key lncRNAs involved in starvation stress and a reference for subsequent in-depth research.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제16권7호
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• pp.2131-2153
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• 2022

To achieve accurate detection of tuberculosis (TB) areas in chest radiographs, we design a chest X-ray TB area detection algorithm. The algorithm consists of two stages: the chest X-ray TB classification network (CXTCNet) and the chest X-ray TB area detection network (CXTDNet). CXTCNet is used to judge the presence or absence of TB areas in chest X-ray images, thereby excluding the influence of other lung diseases on the detection of TB areas. It can reduce false positives in the detection network and improve the accuracy of detection results. In CXTCNet, we propose a channel attention mechanism (CAM) module and combine it with DenseNet. This module enables the network to learn more spatial and channel features information about chest X-ray images, thereby improving network performance. CXTDNet is a design based on a sparse object detection algorithm (Sparse R-CNN). A group of fixed learnable proposal boxes and learnable proposal features are using for classification and location. The predictions of the algorithm are output directly without non-maximal suppression post-processing. Furthermore, we use CLAHE to reduce image noise and improve image quality for data preprocessing. Experiments on dataset TBX11K show that the accuracy of the proposed CXTCNet is up to 99.10%, which is better than most current TB classification algorithms. Finally, our proposed chest X-ray TB detection algorithm could achieve AP of 45.35% and AP50 of 74.20%. We also establish a chest X-ray TB dataset with 304 sheets. And experiments on this dataset showed that the accuracy of the diagnosis was comparable to that of radiologists. We hope that our proposed algorithm and established dataset will advance the field of TB detection.

• Nuclear Engineering and Technology
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• 제54권7호
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• pp.2491-2509
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• 2022

As an important device in the nuclear island, the nuclear coolant pump can continuously provide power for medium circulation. The vane is one of the stationary parts in the nuclear coolant pump, which is installed between the impeller and the casing. The shape of the vane plays a significant role in the pump's overall performance and stability which are the important indicators during the safety serve process. Hence, the bionic concept is firstly applied into the design process of the vane to improve the performance of the nuclear coolant pump. Taking the scaled high-performance hydraulic model (on a scale of 1:2.5) of the coolant pump as the reference, a united bionic design approach is proposed for the unique structure of the guide vane of the nuclear coolant pump. Then, a new optimization design platform is established to output the optimal bionic vane. Finally, the comparative results and the corresponding mechanism are analyzed. The conclusions can be gotten as: (1) four parameters are introduced to configure the shape of the bionic blade, the significance of each parameter is herein demonstrated; (2) the optimal bionic vane is successfully obtained by the optimization design platform, the efficiency performance and the head performance of which can be improved by 1.6% and 1.27% respectively; (3) when compared to the original vane, the optimized bionic vane can improve the inner flow characteristics, namely, it can reduce the flow loss and decrease the pressure pulsation amplitude; (4) through the mechanism analysis, it can be found out that the bionic structure can induce the spanwise velocity and the vortices, which can reduce drag and suppress the boundary layer separation.

• Nuclear Engineering and Technology
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• 제54권7호
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• pp.2720-2727
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• 2022

The Generation IV Lead-cooled fast reactor (LFR) take the liquid lead or lead-bismuth eutectic alloy (LBE) as the coolant of the primary cooling circuit. Combined with the natural characteristics of lead alloy and the design features of LFR, the system is the simplest and the number of equipment is the least, which reflects the inherent safety characteristics of LFR. The nuclear main coolant pump (MCP) is the only power component and the only rotating component in the primary circuit of the reactor, so the various operating characteristics of the MCP are directly related to the safety of the nuclear reactor. In this paper, various working conditions that may occur in the normal rotation (positive rotating) of the MCP and the corresponding internal flow characteristics are analyzed and studied, including the normal pump condition, the positive-flow braking condition and the negative-flow braking condition. Since the corrosiveness of LBE is proportional to the fluid velocity, the distribution of flow velocity in the pump channel will be the focus of this study. It is found that under the normal pump condition and positive-flow braking conditions, the high velocity region of the impeller domain appears at the inlet and outlet of the blade. At the same radius, the pressure surface is lower than the back surface, and with the increase of flow rate, the flow separation phenomenon is obvious, and the turbulent kinetic energy distribution in impeller and diffuser domain shows obvious near-wall property. Under the negative-flow braking condition, there is obvious flow separation in the impeller channel.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제16권12호
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• pp.3904-3922
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• 2022

As a research hotspot, pedestrian detection has a wide range of applications in the field of computer vision in recent years. However, current pedestrian detection methods have problems such as insufficient detection accuracy and large models that are not suitable for large-scale deployment. In view of these problems mentioned above, a lightweight pedestrian detection and early warning method using a new model called you only look once (Yolov5) is proposed in this paper, which utilizing advantages of Yolov5s model to achieve accurate and fast pedestrian recognition. In addition, this paper also optimizes the loss function of the batch normalization (BN) layer. After sparsification, pruning and fine-tuning, got a lot of optimization, the size of the model on the edge of the computing power is lower equipment can be deployed. Finally, from the experimental data presented in this paper, under the training of the road pedestrian dataset that we collected and processed independently, the Yolov5s model has certain advantages in terms of precision and other indicators compared with traditional single shot multiBox detector (SSD) model and fast region-convolutional neural network (Fast R-CNN) model. After pruning and lightweight, the size of training model is greatly reduced without a significant reduction in accuracy, and the final precision reaches 87%, while the model size is reduced to 7,723 KB.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제17권8호
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• pp.1976-1995
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• 2023

The anchor-free object detector CenterNet regards the object as a center point and predicts it based on the Gaussian circle region. For each object's center point, CenterNet directly regresses the width and height of the objects and finally gets the boundary range of the objects. However, the critical range of the object's center point can not be accurately limited by using the Gaussian circle region to constrain the prediction region, resulting in many low-quality centers' predicted values. In addition, because of the large difference between the width and height of different objects, directly regressing the width and height will make the model difficult to converge and lose the intrinsic relationship between them, thereby reducing the stability and consistency of accuracy. For these problems, we proposed a center point prediction method based on the Gaussian elliptic region and a size component regression method based on the small solution space. First, we constructed a Gaussian ellipse region that can accurately predict the object's center point. Second, we recode the width and height of the objects, which significantly reduces the regression solution space and improves the convergence speed of the model. Finally, we jointly decode the predicted components, enhancing the internal relationship between the size components and improving the accuracy consistency. Experiments show that when using CenterNet as the improved baseline and Hourglass-104 as the backbone, on the MS COCO dataset, our improved model achieved 44.7%, which is 2.6% higher than the baseline.

• Mycobiology
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• 제50권5호
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• pp.389-398
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• 2022

Endophytic fungi are promising sources for the production of podophyllotoxin-an important anticancer compound, replacing depleted medical plants. In this study, the endophytes associated with Dysosma difformis-an ethnomedicinal plant species were isolated to explore novel sources of podophyllotoxin. Fifty-three endophytic fungi were isolated and identified by morphological observation and ITS-based rDNA sequencing, assigning them to 27 genera in 3 divisions. Fusarium was found the most prevalent genus with a colonization frequency of 11.11%, followed by Trametes (9.26%) and Penicillium (7.41%). Phylogenetic trees were constructed for the endophytic fungi community in two collection sites, Ha Giang and Lai Chau, revealing the adaptation of the species to the specific tissues and habitats. Cytotoxic activity of endophytic fungal extracts was investigated on cancer cell lines such as SK-LU-1, HL-60, and HepG2, demonstrating strong anti-cancer activity of six isolates belonging to Penicillium, Trametes, Purpureocillium, Aspergillus, and Ganoderma with IC₅₀ value of lower than 10 ㎍/mL. The presence of podophyllotoxin was indicated in Penicillium, Trametes, Aspergillus and for the first time in Purpureocillium and Ganoderma via high-performance liquid chromatography, which implied them as a potential source of this anticancer compound.

• Nuclear Engineering and Technology
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• 제54권11호
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• pp.4072-4083
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• 2022

Ultrasonic impact treatment (UIT) is carried out on the Ni-based alloy stainless steel pipe gas tungsten arc welding (GTAW) girth weld, the differences of microstructure, microhardness and shear strength distribution of the joint before and after ultrasonic shock are studied by microhardness test and shear punch test. The results show that after UIT, the plastic deformation layer is formed on the outside surface of the Ni-based alloy overlayer, single-phase austenite and γ type precipitates are formed in the overlayer, and a large number of columnar crystals are formed on the bottom side of the overlayer. The average microhardness of the overlayer increased from 221 H V to 254 H V by 14.9%, the shear strength increased from 696 MPa to 882 MPa with an increase of 26.7% and the transverse average residual stress decreased from 102.71 MPa (tensile stress) to -18.33 MPa (compressive stress), the longitudinal average residual stress decreased from 114.87 MPa (tensile stress) to -84.64 MPa (compressive stress). The fracture surface has been appeared obvious shear lip marks and a few dimples. The element migrates at the fusion boundary between the Ni-based alloy overlayer and the austenitic stainless steel joint, which is leaded to form a local martensite zone and appear hot cracks. The welded joint is cooled by FA solidification mode, which is forming a large number of late and skeleton ferrite phase with an average microhardness of 190 H V and no obvious change in shear strength. The base metal is all austenitic phase with an average microhardness of 206 H V and shear strength of 696 MPa.

• Korean Journal of Acupuncture
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• 제39권1호
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• pp.8-15
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• 2022

Objectives : Saam acupuncture is one of the indigenous therapeutic modalities in traditional Korean medicine. In this study, the neuroprotective effect of Saam acupuncture of kidney tonification was investigated using 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. Methods : Twelve-week-old male C57BL/6 mice were intraperitoneally administered with 30 mg/kg of MPTP at 24-h intervals for 5 days and acupuncture stimulation at LU8, KI7, SP3 and KI3 was performed once a day for 12 days from the first MPTP injection. The pole test and the rotarod test were performed to evaluate motor function, and dopaminergic neuronal survival in the substantia nigra (SN) and striatum was evaluated using tyrosine-hydroxylase immunohistochemistry. Results : MPTP administration caused behavioral impairment and dopaminergic neuronal death in the nigrostriatal pathway. Whereas the Saam acupuncture treatment alleviated the MPTP-induced motor dysfunction and dopaminergic neuronal death in the SN and striatum. Conclusions : Saam acupuncture of kidney tonification can alleviate the MPTP-induced motor dysfunction and dopaminergic neuronal death in the nigrostriatal pathway, suggesting a possible role for acupuncture in the treatment of Parkinson's disease.

• 한국컴퓨터정보학회논문지
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• 제28권7호
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• pp.179-187
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• 2023

소비자 신뢰는 공유 경제 발전에 중요한 역할을 한다. 본 연구는 소비자 신뢰에 영향을 미치는 요인을 중점적으로 다루며, 중국의 자전거 공유 산업에서 선두였던 오포(OFO)의 사례를 살펴보았다. 본 논문은 오포의 내부 경영 문제와 거의 파산상태에 이르기까지 소비자 신뢰의 하락을 분석합니다. 2018년 12월부터 오포가 직면한 "환불의 어려움" 문제는 계속해서 커져 왔으며, 본 연구는 이 상황을 배경으로 신뢰/불신에 영향을 주는 영향 요인들을 살펴보았다. 제품 요인(품질), 플랫폼 요인(결제 보안, 개인 정보 보호, 명성), 사회적 요인(사회 규범, 정부 규제)을 독립변수로 고려하여 소비자 신뢰에 영향을 미치는 요인을 분석합니다. 분석 결과, 공유 자전거에 대한 소비자의 불신이 커지면 전환의도가 높아지며, 회사의 평판과 사회적규범은 신뢰와 불신에 모두 영향을 미치고, 정부규제는 신뢰에 영향을 미친다는 것을 확인하였다. 연구 결과는 공유 경제 플랫폼에 시사하는 바가 있으며 향후 연구를 위한 지침을 제공할 수 있을 것으로 기대된다.