Mycobiology

The Korean Society of Mycology (한국균학회)
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Isolation and Cytotoxic Potency of Endophytic Fungi Associated with Dysosma difformis, a Study for the Novel Resources of Podophyllotoxin

  • Hoa Thi Tran (Institute of Biotechnology, Vietnam Academy of Science and Technology) ;
  • Giang Thu Nguyen (Institute of Biotechnology, Vietnam Academy of Science and Technology) ;
  • Hong Ha Thi Nguyen (Institute of Biotechnology, Vietnam Academy of Science and Technology) ;
  • Huyen Thi Tran (Institute of Biotechnology, Vietnam Academy of Science and Technology) ;
  • Quang Hong Tran (Vietnam Academy of Science and Technology, Graduate University of Science and Technology) ;
  • Quang Ho Tran (Institute of Biotechnology, Vietnam Academy of Science and Technology) ;
  • Ngoc Thi Ninh (Institute of Marine Biochemistry, Vietnam Academy of Science and Technology) ;
  • Phat Tien Do (Institute of Biotechnology, Vietnam Academy of Science and Technology) ;
  • Ha Hoang Chu (Institute of Biotechnology, Vietnam Academy of Science and Technology) ;
  • Ngoc Bich Pham (Institute of Biotechnology, Vietnam Academy of Science and Technology)
  • Received : 2022.06.27
  • Accepted : 2022.09.14
  • Published : 2022.10.31
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Abstract

Endophytic fungi are promising sources for the production of podophyllotoxin-an important anticancer compound, replacing depleted medical plants. In this study, the endophytes associated with Dysosma difformis-an ethnomedicinal plant species were isolated to explore novel sources of podophyllotoxin. Fifty-three endophytic fungi were isolated and identified by morphological observation and ITS-based rDNA sequencing, assigning them to 27 genera in 3 divisions. Fusarium was found the most prevalent genus with a colonization frequency of 11.11%, followed by Trametes (9.26%) and Penicillium (7.41%). Phylogenetic trees were constructed for the endophytic fungi community in two collection sites, Ha Giang and Lai Chau, revealing the adaptation of the species to the specific tissues and habitats. Cytotoxic activity of endophytic fungal extracts was investigated on cancer cell lines such as SK-LU-1, HL-60, and HepG2, demonstrating strong anti-cancer activity of six isolates belonging to Penicillium, Trametes, Purpureocillium, Aspergillus, and Ganoderma with IC50 value of lower than 10 ㎍/mL. The presence of podophyllotoxin was indicated in Penicillium, Trametes, Aspergillus and for the first time in Purpureocillium and Ganoderma via high-performance liquid chromatography, which implied them as a potential source of this anticancer compound.

Keywords

Acknowledgement

The authors are grateful to Bon Ngoc Trinh from the Vietnamese Academy of Forest Sciences and Thanh Huong Thi Nguyen from the Institute of Ecology and Biological Resources for the identification of D. difformis. We also thank Dr. Thao Thi Do for the cytotoxic assay.

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