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Inhibitory Effects of Ethanolic Extracts from Aster glehni on Xanthine Oxidase and Content Determination of Bioactive Components Using HPLC-UV (섬쑥부쟁이 에탄올 추출물의 잔틴산화효소 저해 효능 및 HPLC-UV를 이용한 유효성분의 함량 분석)

  • Kang, Dong Hyeon;Han, Eun Hye;Jin, Changbae;Kim, Hyoung Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.11
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    • pp.1610-1616
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    • 2016
  • This study aimed to establish an optimal extraction process and high performance liquid chromatography-ultraviolet (HPLC-UV) analytical method for determination of 3,5-dicaffeoylquinic acid (3,5-DCQA) as a part of materials standardization for the development of a xanthine oxidase inhibitor as a health functional food. The quantitative determination method of 3,5-DCQA as a marker compound was optimized by HPLC analysis using a Luna RP-18 column, and the correlation coefficient for the calibration curve showed good linearity of more than 0.9999 using a gradient eluent of water (1% acetic acid) and methanol as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 320 nm. The HPLC-UV method was applied successfully to quantification of the marker compound (3,5-DCQA) in Aster glehni extracts after validation of the method with linearity, accuracy, and precision. Ethanolic extracts of A. glehni (AGEs) were evaluated by reflux extraction at 70 and $80^{\circ}C$ with 30, 50, 70, and 80% ethanol for 3, 4, 5, and 6 h, respectively. Among AGEs, 70% AGE at $70^{\circ}C$ showed the highest content of 3,5-DCQA of $52.59{\pm}3.45mg/100g$ A. glehni. Furthermore, AGEs were analyzed for their inhibitory activities on uric acid production by the xanthine/xanthine oxidase system. The 70% AGE at $70^{\circ}C$ showed the most potent inhibitory activity with $IC_{50}$ values of $77.01{\pm}3.13{\sim}89.96{\pm}3.08{\mu}g/mL$. The results suggest that standardization of 3,5-DCQA in AGEs using HPLC-UV analysis would be an acceptable method for the development of health functional foods.

Quality Evaluation of Juanbyeo as Aseptic-packaged Cooked Rice (무균포장밥 가공 적합품종 주안벼의 식미특성)

  • Oh, Sea-Kwan;Kim, Dae-Jung;Cheun, A-Reum;Yoon, Mi-Ra;Hong, Ha-Cheol;Choi, Im-Soo;Oh, Yea-Jin;Oh, Ki-Back;Kim, Yeon-Kyu
    • Korean Journal of Food Science and Technology
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    • v.42 no.6
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    • pp.721-726
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    • 2010
  • The objective of this study was to evaluate several varieties of aseptic-packaged cooked rice including Juanbyeo. Quality evaluations, such as by amylose, setback, toyo values and amylograms, were significantly higher in the Juanbyeo as compared to other samples. In addition, the Juanbyeo aseptic-packeged cooked rice showed significantly higher palatability characteristics and had a greater water absorption ratio than the other samples. For cold cooked rice, the relationship between palatability and texture was significantly high (p<0.01). Ilpumbyeo showed high scores for taste as a hot cooked rice, but as a cold cooked rice taste scores were lower than other samples. In the case of Juanbyeo cold cooked rice flavor was improved. When the Juanbyeo aseptic-packaged cooked rice was reheated in a microwave oven, it had better palatability than other varieties. Thus, the results of this study suggested that Juanbyeo can be effectively utilized as aseptic-packaged cooked rice.

Comparative Studies on Phytochemicals and Bioactive Activities in 24 New Varieties of Red Pepper (신품종 고추 24종의 phytochemicals 분석 및 생리활성 비교)

  • Huang, Ying;So, Yun-Ji;Hwang, Jeong Rok;Yoo, Kyung-Mi;Lee, Ki-Won;Lee, Yong-Jik;Hwang, In Kyeong
    • Korean Journal of Food Science and Technology
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    • v.46 no.4
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    • pp.395-403
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    • 2014
  • This study aimed to investigate the activities of phytochemical and bioactive components from 24 new varieties of red pepper obtained by crossbreeding different Korean red pepper lines. The ASTA (American Spice Trade Association) color values ranged from 116.69 to 249.83 and the total carotenoid content ranged from 3.19 to 6.11 mg/g dried red pepper. The free sugar content ranged from 43.41 to 199.54 mg/g, and the fructose content was found to be higher than the glucose content. The ascorbic acid, total polyphenol, and flavonoid contents were found to be 8.08-11.53 mg/g, 7.93-14.26 mg gallic acid/g, and 4.24-10.07 mg quercetin/g dried pepper, respectively. Overall, the total polyphenol contents of the new varieties were found to be higher than the average content of Korean red peppers. The total capsaicinoid content in the new varieties varied widely from 0.42 to 237.87 mg/100 g dried pepper. The pepper-methanol extract antioxidant activities of 'variety 2, 4, 8, 9' were higher than others. A high correlation was observed between the antioxidant activities and the polyphenol contents (r=0.804, r=0.701).

Verification of wrinkle improvement effect by animal experiment of suture for skin wrinkle improvement by applying CO2 gas and RF radio frequency (CO2 gas와 RF 고주파를 적용한 피부 주름 개선용 봉합사 동물 실험에 따른 주름 개선 효과 검증)

  • Jeong, Jin-Hyoung;Shin, Un-Seop;Song, Mi-Hui;Lee, Sang-Sik
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.13 no.3
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    • pp.226-234
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    • 2020
  • As the average life expectancy of human beings is extended in addition to the entry of the aging society, there is a tendency for the interest in the appearance of men and women in modern society to increase. The most external judgment of human aging is the wrinkles on the facial skin. People are undergoing various procedures to have clean, wrinkled, and resilient healthy skin. Many thread lifting procedures are being implemented because they tend to want simple and effective procedures during the procedure. In this study, in order to improve lifting effect in thread lifting, animal experiments were conducted to confirm the improvement of wrinkles by injecting RF high frequency and CO2 gas into existing PDO suture procedures. The experimental groups consisted of natural aging groups, PDO treatment groups, groups with RF high frequency in PDO procedures, groups with CO2 gas injected into PDO procedures, and groups with CO2 gas and RF injected simultaneously into PDO procedures. The individuals in the natural aging group had an average wrinkle depth of 0.408mm before the procedure, and the average wrinkle depth of the 10th week was 0.68mm. The depth of wrinkles in the PDO treatment group averaged 0.384mm before the procedure, and 0.348mm on the 10th week after the procedure. The average crease depth of pre-procedure objects injected with RF high frequency in PDO was 0.42mm, and the average crease depth for 10 weeks was 0.378mm. The average crease depth of the CO2 gas injected into the PDO was 0.4mm before the procedure, and the average crease depth was reduced to 0.332mm in the 10th week after the procedure. On average, the number of objects injected with CO2 gas and RF high frequency in the PDO procedure decreased to 0.412mm before and 0.338mm in the 10th week after the procedure. The procedure of injecting CO2 gas and RF into the PDO suture showed the highest reduction rate of 17.96%.

Public Park Awareness along with Community Garden Cultivation Participation within an Urban Park (도시공원 내 텃밭 경작 참여 여부에 따른 공원 공공성 인식 연구)

  • Nam Koong, Hee;Kim, Seul-Yea;Kang, Eun-Jee;Kim, Yong-Geun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.43 no.1
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    • pp.120-131
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    • 2015
  • This research aimed at understanding recognition of the park's community garden, public evaluation of it, and user awareness of the public enhancement plan. This was based on the problem that arose in the park community garden as public awareness research along with community garden cultivation participation within the urban park. In order to compare recognition differences along with community garden cultivation participation, the researcher conducted an analysis by dividing research participants into a cultivation participant group and non-participant group. As a result first, the cultivation participant group positively recognized the necessity of the community garden compared to the non-participant group. However, they recognized the community garden as a space for private profit, which threatened the public aspect of the park. Second, as a result of evaluating community garden publicness, the non-participant group which was alienated from community garden use, had a negative opinion about the community garden. Third, as a result of researching awareness of the community garden distribution method and consumption to promote publicness of the community garden, the cultivation participant group recognized the community garden as a space for private profit. Therefore, it is necessary for both groups to be aware of the public value of the community garden among the park users for sustainable management and operation. The significance of this research is the direction in the aspect of users to form, manage and operate the community garden in the urban park without damaging publicness of the park or conflicting between the function of the park and the function of the community garden. Henceforth, the researcher expects that this research can be utilized to maintain publicness of the park in respect of formation of the community garden in the park and management and operation.

EVALUATION ON THE ABRASION RESISTANCE OF A SURFACE SEALANT (레진전색제의 마모저항성에 대한 평가)

  • Kim, Soo-Mee;Han, Sae-Hee;Cho, Young-Gon
    • Restorative Dentistry and Endodontics
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    • v.32 no.3
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    • pp.180-190
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    • 2007
  • The purpose of this study was to evaluate the abrasion resistance of surface penetrating sealant which was applied on a composite resin restoration and to provide proper time to reapply sealant on composite resin surface. Two hundred rectangular specimens, sized $8\times3\times2mm$, were made of Micronew (Bisco, Inc., Schaumburg, IL, U.S.A) and divided into two groups; F group (n = 10) was finished with coarse and medium grit of Sof-Lex discs and BisCoverwas applied B group (n = 190) after finishing with discs. B group was again subdivided into nineteen subgroups From B-1 group to B-18 group were subjected to toothbrush abrasion test using a distilled water-dentifrice slurry and toothbrush heads B-IM group was not subjected to toothbrush abrasion test. Average surface roughness (Ra) of each group was calculated using a surface roughness tester (Surfcorder MSE-1700: Kosaka Laboratory Ltd., Tokyo, Japan) . A representative specimen of each group was examined by FE-SEM (S-4700: Hitachi High Technologies Co., Tokyo, Japan). The data were analysed using cluster analysis, paired t-test, and repeated measure ANOVA. The results of this study were as follows; 1. Ra off group was $0.898{\pm}0.145{\mu}m$ and B-IM group was $0.289{\pm}0.142{\mu}m$. Ra became higher from B-1 group $(0.299{\pm}0.48{\mu}m$ to B-18 group $(0.642{\pm}0.313{\mu}m$. 2. Final cluster center of Ra was $0.361{\mu}m$ in cluster 1 $(B-IM\simB-7)$, $0.511{\mu}m$ in cluster 2 $(B-8\simB-14)$ and $0.624{\mu}m$ in cluster 3 ($(B-15\simB-18)$. There were significant difference among Ra of three clusters. 3 Ra of B-IM group was decreased 210.72% than Ra of F group. Ra of B-8 group and B-15 group was increased 35.49% and 51.35% respectively than Ra of B-IM group. 4. On FE-SEM, B-IM group showed the smoothest resin surface. B-8 group and B-15 group showed vertically shallow scratches , and wide and irregular vertical scratches on composite resin surface respectively. Within a limitation of this study, finished resin surface will be again smooth and glazy if BisCover would be reapplied within 8 to 14 months after applying to resin surface.

A Study on the Status and Management Plan of Naturalized Plant in Area of Scenic Site at Mt. Maisan, Jinan (진안 마이산 명승구역 내 귀화식물 현황 및 관리방안)

  • Rho, Jae-Hyun;Oh, Hyun-Kyung;Han, Sang-Yub;Choi, Yung-Hyun;Kim, Eun-Ok
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.36 no.3
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    • pp.100-114
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    • 2018
  • Nationally designated Cultural Properties 'Scenic site No.12 Maisan Mountain, Jinan' designated areas and some protected areas, and taking into account the dynamics of naturalized plants causing problems, we will restore the original vegetation scenery of Mt. Maisan. The results of this study are as follows. A total of 76 families, 192 genera, 286 taxa, and inland and inhabited areas, 76 and 138 genera and 163 taxa were identified in the areas of Ammaibong. The total number of naturalized plants identified in this study area is 28 taxa total, which corresponds to 7.1% naturalization rate(NR) among the vascular plants of all 395 taxa, and the urbanization index(UI) corresponds to 8.4% of the 333 taxa of Korean naturalized plants. Ecosystem disturbance plants identified in the survey area were Ambrosia artemisiifolia 1 taxa. The naturalized plants controlled and managed by separate anthropogenic vegetation management within the designation and protection area of Maisan scenic place are three species of herbaceous Rumex acetosella, A. artemisiifolia and Festuca arundinacea. It was identified as a breed. Indigofera bungeana and F. arundinacea communities around the stairway and Amorpha fruticosa, I. bungeana, A. artemisiifolia and Amaranthus patulus of the top of Am-Maibong were selected as the first priority sites for exclusion of exotic species in Maisan area and target naturalized plants species to the Ammaibong peak. In addition, R. acetosella community around the temple was suggested to be removed first to preserve endemic species. For the restoration of vegetation, we suggest that Stephanandra incisa, Spiraea blumei, Weigela subsessilis, etc. should be planted after removal of I. bungeana, and F. arundinacea, C. lanceolata, Carex callitrichos var. nana.

Influence of additional etching on shear bond strength of self-etching adhesive system to enamel (부가적인 산부식이 자가산부식 접착제의 법랑질에 대한 전단결합강도에 미치는 영향)

  • Yoo Sun-Jin;Kim Young-Kyung;Park Jeong-Won;Jin Myoung-Uk;Kim Sung-Kyo
    • Restorative Dentistry and Endodontics
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    • v.31 no.4
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    • pp.263-268
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    • 2006
  • Recently, self-etching adhesive system has been introduced to simplify the clinical bonding proce- dures. It is less acidic compared to the phosphoric acid, thus there is doubt whether this system has enough bond strength to enamel. The purpose of this study was to investigate the influence of additional etching on the adhesion of resin composite to enamel. Ninety extracted bovine permanent anterior teeth were used. The labial surfaces of the crown were ground with 600-grit abrasive paper under wet condition. The teeth were randomly divided into six groups of 15 teeth each. Clearfil SE $Bond^{\circledR},\;Adper^{TM}$ Prompt L-Pop and Tyrian $SPE^{TM}$ were used as self-etching primers. Each self-etching primers were applied in both enamel specimens with and without additional etching. For additional etching groups, enamel surface was pretreated with 32% phosphoric acid (UNI-ETCH, Bisco, Inc., Schaumburg, IL. USA). Hybrid resin composite Clearfil AP-X, (Kuraray Co., Ltd., Osaka, Japan) was packed into the mold and light-cured for 40 seconds. Twenty-four hours after storage, the specimens were tested in shear bond strength. The data for each group were subjected to independent t - test at p < 0.01 to make comparisons among the groups. In Clearfil SE $Bond^{\circledR}$, shear bond strength of additional etching group was higher than no additional etching group (p < 0.01). In $Adper^{TM}$ Prompt L-Pop and Tyrian SPE, there were no significant difference between additional etching and non-etching groups (p > 0.01). In conclusion, self-etching adhesive system with weak acid seems to have higher bond strength to enamel with additional etching, while self-etching adhesive system with strong acid seems not.

COLOR DIFFERENCES BETWEEN RESIN COMPOSITES BEFORE- AND AFTER-POLYMERIZATION, AND SHADE GUIDES (복합레진의 광중합 전·후와 shade guide의 색차 비교)

  • Chon, Yi-Ju;Cho, Sung-Shik;Um, Chung-Moon
    • Restorative Dentistry and Endodontics
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    • v.24 no.2
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    • pp.299-309
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    • 1999
  • The composite resin, due to its esthetic qualities, is considered the material of choice for restoration of anterior teeth. With respect to shade control, the direct-placement resin composites offer some distinct advantages over indirect restorative procedures. Visible-light-cured (VLC) composites allow dentists to match existing tooth shades or to create new shades and to evaluate them immediately at the time of restoration placement. Optimal intraoral color control can be achieved if optical changes occurring during application are minimized. An ideal VLC composite, then, would be one which is optically stable throughout the polymerization process. The shade guides of the resin composites are generally made of plastic, rather than the actual composite material, and do not accurately depict the true shade, translucency, or opacity of the resin composite after polymerization. So the numerous problems associated with these shade guides lead to varied and sometimes unpredictable results. The aim of this study was to assess the color changes of current resin composite restorative materials which occur as a result of the polymerization process and to compare the color differences between the shade guides provided with the products and the actual resin composites before- and after-polymerization. The results obtained from this investigation should provide the clinician with information which may aid in improved color match of esthetic restoration. Five light activated, resin-based materials (${\AE}$litefil, Amelogen Universal, Spectrum TPH VeridonFil-Photo, and Z100) and shade guides were used in this study. Three specimens of each material and shade combination were made. Each material was condensed inside a 1.5mm thick metal mold with 10mm diameter and pressed between glass plates. Each material was measured immediately before polymerization, and polymerized with Curing Light XL 3000 (3M Dental products, USA) visible light-activation unit for 60 seconds at each side. The specimens were then polished sequentially on wet sandpaper. Shade guides were ground with polishing stones and rubber points (Shofu) to a thickness of approximately 1.5mm. Color characteristics were performed with a spectrophotometer (CM-3500d, Minolta Co., LTD). A computer-controlled spectrophotometer was used to determine CIELAB coordinates ($L^*$, $a^*$ and $b^*$) of each specimen and shade guide. The CIELAB measurements made it possible to evaluate the amount of the color difference values (${\Delta}E{^*}ab$) of resin composites before the polymerization process and shade guides using the post-polishing color of the composite as a control, CIE standard D65 was used as the light source. The results were as follows. 1. Each of the resin composites evaluated showed significant color changes during light-curing process. All the resin composites evaluated except all the tested shades of 2100 showed unacceptable level of color changes (${\Delta}E{^*}ab$ greater than 3.3) between pre-polymerization and post-polishing state. 2. Color differences between most of the resin composites tested and their corresponding shade guides were acceptable but those between C2 shade of ${\AE}$litefil and IE shade of Amelogen Universal and their respective shade guides exceeded what is acceptable. 3. Comparison of the mean ${\Delta}E{^*}ab$ values of materials revealed that Z100 showed the least overall color change between pre-polymerization and post-polishing state followed by ${\AE}$litefil, VeridonFil-Photo, Spectrum TPH, and Amelogen Universal in the order of increasing change and Amelogen Universal. Spectrum TPH, 2100, VeridonFil-Photo and ${\AE}$litefil for the color differences between actual resin and shade guide. 4. In the clinical environment, the shade guide is the better choice than the shade of the actual resin before polymerization when matching colors. But, it is recommended that custom shade guides be made from resin material itself for better color matching.

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Comparison of Fragrance and Chemical Composition of Essential Oils in Gom-chewi (Ligularia fischeri) and Handaeri Gom-chewi (Ligularia fischeri var. spicifoprmis) (곰취(Ligularia fischeri)와 한대리곰취(Ligularia fischeri var. spicifoprmis) 정유의 향취 및 향기성분 비교)

  • Yeon, Bo-Ram;Cho, Hae Me;Yun, Mi Sun;Jhoo, Jin-Woo;Jung, Ji Wook;Park, Yu Hwa;Kim, Songmun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.12
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    • pp.1758-1763
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    • 2012
  • This study was conducted to compare fragrance and volatile chemicals of essential oils in Gom-chewi (Ligularia fischeri) and Handaeri Gom-chewi (Ligularia fischeri var. spicifoprmis). Essential oils were extracted by steam distillation of leaves of Gom-chewi (GC) and Handaeri Gom-chewi (HGC), after which samples were collected by solid-phase micro extraction and the compositions of the essential oils were analyzed by gas chromatography-mass spectrometry (GC-MS). The yields of the essential oils in GC and HGC were 0.12% and 0.04%, respectively, and the threshold levels of the essential oils in GC and HGC were 0.01% and 0.1%, respectively. There were 19 constituents of the essential oil of Gom-chewi: 14 carbohydrates, 4 alcohols, and 1 acetate, and the major constituents were L-${\beta}$-pinene (36.02%), D-limonene (25.64%), ${\alpha}$-pinene (24.85%) and ${\beta}$-phellandrene (5.39%). In the essential oil of HGC, 25 constituents were identified: 17 carbohydrates, 4 alcohols, 3 acetates, and 1 N-containing compound, and the major constituents of HGC were D-limonene (39.74%), L-${\beta}$-pinene (35.43%) and ${\alpha}$-pinene (11.94%). The minor constituents of HGC were ${\rho}$-cymene, ${\gamma}$-muurolene, ${\gamma}$-cadinene, germacrene D, ingol 12-acetate and butyl 9,12,15-octadecatriene and nimorazole were not identified in the GC essential oil. Overall, the results showed that the fragrance and chemical compositions of essential oils in GC and HGC differed, suggesting that both essential oils could be used for the development of perfumery products.