• Title/Summary/Keyword: LS-1

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Ethanol Production an Immobilized Themotolerant Mutant of Brettanomyces custersii H1-39 from Wood Hydrolyzate Media (목질계 당화액배지로부터 고온내성 변이주 Brettanomyces custersii H1-39의 고정화에 의한 에탄올생산)

  • 박승원;홍영기;김승욱;홍석인
    • Microbiology and Biotechnology Letters
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    • v.28 no.3
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    • pp.172-179
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    • 2000
  • Bretlanomyces C!tstersii Hl-39 mutant was immobilized with various caniers. Immobilized mutant Hl-39 produced more ethanol and showed higher productivity and cell concentration than those of free 81-39 in 3.4% hydrolyzate of wood-chips at different temperatures ($37^{\circ}C$, $40^{\circ}C$ and $43^{\circ}C$). At $37^{\circ}C$, ethanol concentration produced by mutant H1-39 immobilized in Ca-alginate and ARG(l % Ca-alginate, 1.67% bentonite, 0.33% glutaraldehyde) bead were higher than those produced by the other earners (ACG ; 1 % CaHalginate. ] .67% celite R-634 , 0.33% glutaraldehyde, ABP ; 1 % Ca-alginate. 1.67% bentonite, 0.33% pectin. ACP: 1 % Ca-alginate, ] .67% celiLe R-634, 0.33% pecLin). The highest value of productivity(l.23 ) was obtained by using ABG beads. At $40^{\circ}C$, ethanol conccntration and productivity obtained by ABC beads ,>,"ere 15.2 glL and 0.84 gl L.h, respectively, which showed the highest value compared to other carriers. Particularly, productivity of ilmnobilized ceIl was increased up to 90% as compared to that offree cell. On the other hand, ABP(l % Ca-alginate+L67% bentonile+O.33% pectin) beads gave the best resulLs at $43^{\circ}C$ for production of ethanol and productivity, which were 13.8 g!l and 0.77 g/l h, respectively.ively.

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Changes in lip and periornl soft tissue after bracket removal (브라켓 제거에 따른 입술과 주위 연조직의 변화)

  • Lee, Jeong-Sub;Choy, Kwang-Chul;Park, Young-Chel;Kim, Kyung-Ho
    • The korean journal of orthodontics
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    • v.37 no.2 s.121
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    • pp.125-136
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    • 2007
  • Objective: Soft tissue changes due to orthodontic treatment has large individual variation. Therefore continuous evaluation during treatment is required. Patients with fixed orthodontic appliances often wonder if their lip positions will change after the removal of brackets, but only a few studies exist on this topic. The objective of this study was to evaluate the changes of the lips and perioral soft tissue after bracket removal. Methods: The sample used in this study was 19 males and 33 females. Cephalometric X-rays were taken at 3 stages - T1 (before debonding), T2 (just after debonding), T3 (1.5 months after debonding). Results: The lower lip was retruded immediately after debonding (T2-T1), and 1.5 months after debonding (T3-T2). The mean amounts of retrusion from the vertical reference plane (sG perpendicular line) were about 0.38 mm for the upper lip and 0.88 mm for the lower lip. Immediately after debonding, lip retrusion of females was greater than that of males. During the post-debonding period, lower lip of males was retruded more than that of females. Conclusion: Lips are retruded after bracket removal, and there is no gender difference 1.5 months after debonding.

Comparative Analysis of SWAT Generated Streamflow and Stream Water Quality Using Different Spatial Resolution Data (SWAT모형에서 다양한 해상도에 따른 수문-수질 모의결과의 비교분석)

  • Park, Jong-Yoon;Lee, Mi-Seon;Park, Geun-Ae;Kim, Seong-Joon
    • Proceedings of the Korea Water Resources Association Conference
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    • 2008.05a
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    • pp.102-106
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    • 2008
  • This study is to evaluated the impact of varying spatial resolutions of DEM (2 m, 10 m, and 30 m), land use (QuickBird, 1/25,000 and Landsat), and soil data (1/25,000 and 1/50,000) on the uncertainty of Soil and Water Assessment Tool (SWAT) predicted streamflow, sediment, T-N, and T-P transport in a small agricultural watershed ($1.21\;km^2$). SWAT model was adopted and the model was calibrated for a $255.4\;km^2$ watershed using 30 m DEM, Landsat land use, and 1/25,000 soil data. The model was run with the combination of three DEM, land use, and soil map respectively. The SWAT model was calibrated for 2 years (1999-2000) using daily streamflow and monthly water quality (SS, T-N, T-P) records from 1999 to 2000, and verified for another 2 years (2001-2002). The average Nash and Sutcliffe model efficiency was 0.59 for streamflow and the root mean square error were 2.08, 4.30 and 0.70 tons/yr for sediment, T-N and T-P respectively. The hydrological results showed that output uncertainty was biggest by spatial resolution of land use. Streamflow increase the watershed average CN value of QucikBird land use was 0.4 and 1.8 higher than those of 1/25,000 and Landsat land use caused increase of streamflow.

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Genetic polymorphisms of monoamine oxidase(MAO) B and serotonin transporter receptor(5-HTTR) influence variability in neurobehavioral symptoms by n-hexane exposur (n-Hexane 노출에 의한 신경행동학적 증상에 monoamine oxidase(B)와 serotonin transporter receptor 유전자다양성의 영향)

  • Kim, Ki-Woong
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.21 no.2
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    • pp.116-122
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    • 2011
  • Human occupational exposure to n-hexane has been associated with neurobehavioral symptoms such as depression, irritablity, acute irritation symptom, concentration disturbance and fatigue. Effects of monoamine oxidase (MAO) B and serotonin transporter receptor (5-HTTR) polymorphisms on the neurobehavioral symptoms were investigated in 70 male workers from TV and computer monitor manufacturing plants exposed to n-hexane. Neurobehavioral symptoms were assessed through a self-reported questionnaire and ambient level of n-hexane was measured by NIOSH method. Blood and urine were collected from each workers to determine the MAO(B), 5-HTTR and urinary 2,5-hexanedione(2,5-HD). The mean concentration of volatile n-hexane was $18.8{\pm}28.8ppm$ and that of urinary 2,5-HD was $1.07{\pm}1.47mg/g$ creatinine. Statistically significant associations with sexual disturbance were age and smoking. The frequencies of MAO(B) AA, AG and GG were 18.6%, 45.7% and 35.7%, respectively, and the frequencies of 5-HTTR ll, ls and ss genotype were 82.9%, 15.7% and 1.4%, respectively. MAO (B) gene polymorphisms had susceptibility to the neurobehavioral symptoms such as fatigue, concentration disturbance, irritability and acute irritation symptom and 5-HTTR gene polymorphism had susceptibility to the sleep disturbance and acute irritation symptom. On multiple logistic regression analysis for the neurobehavioral symptoms, memory disturbance was significantly associated with smoking(OR=6.752, 95% CI=37.46) and drinking(OR=4.033, 95% CI=1.252-12.98), emotional lability was MAO(B) genotype(OR=0.412, 95% CI=0.170-0.996), fatigue (OR=1.011, 95% CI=1.000-1.021) and acute irritation(OR=0.990, 95% CI=0.981-1.000) were working duration and sexual disturbance were significantly associated with age(OR=1.208, 95% CI=1.042-1.399), ambient n-hexane(OR=1.077, 95% CI=1.005-1.154) and 2,5-HD(OR=0.186, 95% CI=0.041-0.841). This finding implies that the MAO (B) and 5-HTTR polymorphisms may affect susceptibility for specific neurobehavioral symptoms associated with n-hexane exposure in workers.

Preparation and Characterization of PVA/PAM Electrolyte Membranes Containing Silica Compounds for Direct Methanol Fuel Cell Application (실리카 화합물을 함유한 PVA/PAM 전해질 막의 제조 및 특성과 직접메탄올 연료전지로의 응용)

  • Yoon, Seok-Won;Kim, Dae-Hoon;Lee, Byung-Seong;Lee, Bo-Sung;Moon, Go-Young;Byun, Hong-Sik;Rhim, Ji-Won
    • Polymer(Korea)
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    • v.34 no.1
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    • pp.45-51
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    • 2010
  • This study focuses on the investigation of the possibility of the crosslinked poly (vinyl alcohol) membranes with both poly (acrylic acid-co-maleic acid) (PAM) and 3-(trihydroxysilyl)-1-propane-sulfonic acid (THS-PSA) for the direct methanol fuel cell application. In order to characterize the prepared membranes, the water content, the thermal gravimetric analysis, the ion exchange capacity, the ion conductivity and the methanol permeability measurements were carried out and then compared with the existing Nafion 115 membrane. The ion exchange capacity of the resulting membranes showed 1.6~1.8 meq./g membrane which was improved than Nafion 115, 0.91 meq./g membrane. In the case of the proton conductivity, the THS-PSA introduced membranes gave more excellent $0.042{\sim}0.056\;S{\cdot}cm^{-1}$ than Nafion 115, $0.024\;S{\cdot}cm^{-1}$. On the other hand, the methanol permeability was increased more than Nafion 115 for all the range of THA-PSA concentration.

Infiltration and Percolation Characteristics of Water in Agricultural Land Filled with Rock-Dust (암분 매립 농경지 토양의 표면 침투 및 삼투 특성)

  • Hur, S.O.;Jeon, S.H.;Lee, Y.J.;Han, K.H.;Jo, H.R.;Kang, S.S.;Kim, M.S.;Ha, S.G.;Kim, J.G.
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.spc
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    • pp.40-44
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    • 2009
  • This study was carried for the understanding of infiltration and percolation characteristics of water in agricultural land filled with rock-dust (Technosols). The experiment was performed at two sites (A, B), and soil horizons of the sites were classified with 4 layers, respectively. The soil texture of all soil horizons was analyzed with silt loam (SiL) except for the soil texture, which was loamy sand (LS), at the lowest horizon of measurement site A. The bulk densities at each horizon of two soils were mostly over $1.49g{\cdot}cm^{-3}$, which is very higher than $1.25g{\cdot}cm^{-3}$ of typical medium-textured mineral soil, except for the surface of site A measured immediately after tillage. The concentrations of $P_2O_5$ at surface of two soils s were 1962 (A), 1613 (B) $mg{\cdot}kg^{-1}$, respectively. These concentrations are 3.2~6.5 times of $300{\sim}500mg{\cdot}kg^{-1}$, which is the optimum concentration for crop growth. Infiltration rates at surface of the soils were 3.54 (A), 2.85 (B) cm $hr^{-1}$, but percolation rates at soil horizons under the surface were below 0.3 (A), below 0.003 (B) cm $hr^{-1}$. These results would be because the surface soils were managed by tillage and crop planting etc., but soils under surface were formed with structural problems occurred at the formation time of agricultural land accumulated with rock-dust or a compaction by farm machines.

A truncated form of human alpha 1-acid glycoprotein is useful as a molecular tool for insect glycobiology

  • Morokuma, Daisuke;Hino, Masato;Tsuchioka, Miho;Masuda, Akitsu;Mon, Hiroaki;Fujiyama, Kazuhito;Kajiura, Hiroyuki;Kusakabe, Takahiro;Lee, Jae Man
    • International Journal of Industrial Entomology and Biomaterials
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    • v.36 no.1
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    • pp.15-24
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    • 2018
  • N-glycosylation is an important posttranslational modification that results in a variety of biological activities, structural stability, and protein-protein interactions. There are still many mysteries in the structure and function of N-glycans, and detailed elucidation is necessary. Baculovirus expression system (BES) is widely used to produce recombinant glycoproteins, but it is not suitable for clinical use due to differences in N-glycan structure between insects and mammals. It is necessary to develop adequate model glycoproteins for analysis to efficiently alter the insect-type N-glycosylation pathway to human type. The previous research shows the recombinant alpha 1-acid glycoprotein (${\alpha}1AGP$) secreted from silkworm cultured cells or larvae is highly glycosylated and expected to be an excellent research candidate for the glycoprotein analysis expressed by BES. Therefore, we improved the ${\alpha}1AGP$ to be a better model for studying glycosylation. The modified ${\alpha}1AGP$ (${\alpha}1AGP{\Delta}$) recombinant protein was successfully expressed and purified by using BES, however, the expression level in silkworm cultured cells and larvae were lower than that of the ${\alpha}1AGP$. Subsequently, we confirmed the detailed profile of N-glycan on the ${\alpha}1AGP{\Delta}$ by LS/MS analysis the N-glycan structure at each glycosylation site. These results indicated that the recombinant ${\alpha}1AGP{\Delta}$ could be usable as a better model glycoprotein of N-glycosylation research in BES.

Soil CEC for Textural Classes in Korea (우리나라 토양(土壤)의 토성별(土性別) 양(陽)이온 치환용량(置換容量))

  • Hyeon, Geun-Soo;Park, Chang-Seo;Jung, Sug-Jae;Rim, Sang-Kyu;Um, Ki-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.1
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    • pp.10-16
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    • 1991
  • Mean values and the relative contribution of OM and clay to total CEC for soil textural classes were obtained from the analytical results of the typical profiles(sample size : 3,182) which were described by the detailed soil maps througthout Korea with an exception of Jeju island. The results are below. 1. Mean values of the soil CEC were 2.9 for S, 4.7 for LS, 6.7 for SL, 9.0 for L, 10.2 for SiL, 10.7 for CL, 8.6 for SCL, 12.2 for SiCL, 16.1 for SiC, and 17.4me/100gr for C, respectively. 2. The multiple regression equation and partial regression coefficient tended to show that OM and clay had the highly significant effect on CEC. 3. Clay content of the coarse, moderately coarse, and moderately fine soil was 1.10 to 1.89 times as important as OM content whereas OM of the medium and fine soil 1.09 to 2.94 times as important as clay in predicting CEC. 4. Mean values of CEC of the humus and clay in Korean soils were about 62.9 and 24.0me/100gr, respectively.

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ANALYSIS OF FACIAL SOFT-TISSUE CHANGES AFTER MANDIBULAR SAGITTAL SPLIT RAMUS OSTEOTOMY (하악 전돌증 환자의 하악지 시상 골절단술후 경조직 변화에 따른 안모 연조직 변화 분석)

  • Park, Hee-Dae;Kwon, Tae-Geon;Lee, Sang-Han
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.18 no.1
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    • pp.87-108
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    • 1996
  • This study was intended to perform cephalometric analysis of the facial soft tissue profile after surgical correction of skeletal Class III malocclusion after SSRO in 29 patients (Males 12, females 17). Lateral cephalograms were taken in centric occlusion before and immediate, long term after surgeries. 1. Counter-clockwise rotation of mandible was observed after the surgery, average relapses of mandibular set back were 1.23-1.28mm. The net effects of the mandibular set-back after surgeries were 81.7-82.2%. Because these relapse tendencies may reduce the effects of the surgical outcomes, surgeon must consider these net before the surgical treatment planning. 2. The ratio of horizontal changes of hard tissue to soft tissue at lower lip, mentolabial sulcus, pogonion were 72.7-93.7%, 100.3%, 99.1-102.1% respectively. There were little changes at upper lip position anteroposteriorly. 3. The relationship of upper and lower lips were improved after surgery. Lower lip was posteriorly repostioned and upper lip was flattend and elongated in conjunction with deepening of inferior lobial sulcus. But profile of chin was still prominent after surgery. 4. Hard tissue horizontal changes and tissue vertical changes were significantly correlated with each other and there were reverse correlations with hard tissue vertical changes and soft tissue horizontal changes.

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Establishment of Plant Regeneration from Apical Meristem of Sweetpotato (고구마 정단분열조직 유래 식물체 재분화 조건 확립)

  • Lee, Joon-Seol;Ahn, Young-Sup;Chung, Mi-Nam;Kim, Hag-Sin;Jeong, Byeong-Choon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.233-236
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    • 2006
  • This study was conducted to investigate somatic embryogenesis capacity using callus derived from bud meristems in sweetpotato. Shoot apical meristem explants $(height:150{\mu}m;base:\;350{\mu}m)$were cultured on MS medium supplemented with 1 mg/L 2/4-D. Embryogenic callus were observed in five cultivars when their shoot apices were cultured on MS medium supplements with 1 mg/L 2,4-D. After 6 weeks of culture, greater than 80% of the survived explants produced embryogenic calli and the calli gave rise to somatic embryos at frequencies of 72% (Yulmi), 60% (Shinhwangmi), 78% (Geonmi), 70% (KoKei 14), 40% (Sinjami). The regenerated plants developed into whole plantlets after they were transferred onto the fresh hormon-free MS medium of 74% (Yulmi), 82% (Shinhwangmi), 86% (Geonmi), 74% (Kokei 14), 41% (Sinjami) respectively.