• Genomics & Informatics
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• 제6권1호
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• pp.8-13
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• 2008

High-density lipoprotein (HDL) whose primary role is to transport cholesterol from peripheral tissues to the liver, is associated with the incidence of coronary heart disease. We analyzed HDL cholesterol levels in a genetically isolated population of extended Mongolian families. A total of 1002 individuals (54.5% women) from 95 families were enrolled. After genotyping by use of 1000 microsatellite markers, we performed a genome-wide linkage search with variance component analysis. The estimated heritability of HDL cholesterol was 0.45, revealing that HDL cholesterol was under significant genetic influence. We found peak evidence of linkage (LOD score=1.88) for HDL cholesterol level on chromosome 6 (nearest marker D6S1660) and potential evidences for linkage on chromosomes 1, 12 and 19 with the LOD scores of 1.32, 1.44 and 1.14, respectively. These results should pave the way for the discovery of the relevant genes by fine mapping and association analysis.

• Genomics & Informatics
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• 제9권4호
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• pp.143-151
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• 2011

In this study, we propose a novel, intuitive method of constructing an expression quantitative trait (eQT) network that is related to the metabolic syndrome using LOD scores and peak loci for selected eQTs, based on the concept of gene-gene interactions. We selected 49 eQTs that were related to insulin resistance. A variance component linkage analysis was performed to explore the expression loci of each of the eQTs. The linkage peak loci were investigated, and the "support zone" was defined within boundaries of an LOD score of 0.5 from the peak. If one gene was located within the "support zone" of the peak loci for the eQT of another gene, the relationship was considered as a potential "directed causal pathway" from the former to the latter gene. SNP markers under the linkage peaks or within the support zone were searched for in the database to identify the genes at the loci. Two groups of gene networks were formed separately around the genes IRS2 and UGCGL2. The findings indicated evidence of networks between genes that were related to the metabolic syndrome. The use of linkage analysis enabled the construction of directed causal networks. This methodology showed that characterizing and locating eQTs can provide an effective means of constructing a genetic network.

• 한국식품위생안전성학회지
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• 제31권5호
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• pp.311-318
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• 2016

국내 미지정 색소인 azorubine에 대한 우리나라의 공인분석법을 설립하기 위해 제외국의 공인분석법들(유럽연합의 EFSA, 영국의 FSA, 우리나라의 식품의약품안전평가원)이 비교되었다. 재현된 HPLC 방법들 중 FSA의 분석법이 가장 우수한 분석 결과를 나타내어 azorubine분석법으로 최종 선정되었다. Azorubine 분석을 위한 위 세 기관의 전처리 방법은 모두 낮은 회수율을 나타내었다. 따라서 식품의약품안전평가원의 전처리법을 개선한 새로운 전처리 방법이 개발되었다. 최종 선정된 HPLC 분석법과 본 연구를 통해 개발된 새로운 식품 전처리법에 대한 분석법 밸리데이션을 실시하였다. 검증 실험에서 음료류에서 97~103%, 빵 및 과자류에서 95~101%, 기타 식품에서 93~102%의 회수율을 나타내었고, 실험실간 교차검증에서 -1.29~0.26의 z-score를 보여 신뢰할 수 있는 것으로 판단되었다. 이 결과로부터 본 연구에서 확립된 전처리법 및 분석법은 미지정 색소 azorubine이 사용된 식품검사에 활용될 수 있는 분석법으로 확인되었다.

• 한국육종학회지
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• 제41권4호
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• pp.474-481
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• 2009

본 연구에서는 자포니카형인 '낙동'과 통일형인 '삼강' 조합의 DH 집단을 이용하여 식미를 결정하는 특성 중 하나인 호응집성과 미립의 이화학적 특성인 천립중, 장폭비, 아밀로즈 함량, 단백질 함량, 지질 함량 및 전분 함량 간 상관관계를 검정하였다. 또한 호응집성의 QTL을 분석하고, 각 QTL에 속하는 DNA marker와 DH 집단의 호응집성 및 품종별 호응집성 간 관계를 분석하였다. '삼강/낙동' DH 집단의 호응집성 범위는 35~94 mm로 비교적 넓은 범위의 변이를 나타내었고 양친의 범위를 벗어나는 초월분리현상을 나타내었다. '삼강/낙동' DH 집단에서 호응집성은 미립의 이화학적특성 중 아밀로즈 함량과는 연으로 분류된 계통에서만 부의 유의성 있는 상관관계를 나타내었고, 지질 함량과는 중 및 전체 계통에서는 부의 상관관계를 나타내었으나 연으로 분류된 계통과는 정의 상관관계를 나타내었다. 단백질 함량과는 연으로 분류된 계통과는 정의 상관관계, 중과 전체 계통에서는 부의 상관관계를 나타내었으며, 전분 함량에서는 연으로 분류된 계통에서만 호응집성과 부의 상관관계를 나타내었다. 호응집성과 연관된 QTL은 4번(qgc4)과 11번(qgc11) 염색체에서 탐색되었으며 LOD score는 각각 3.1, 2.9를 나타내었고 두 QTL의 설명 가능한 표현형 변이는 23%로 비교적 크게 작용하고 있었다. '삼강/낙동' DH 집단에서 gel의 길이가 긴 상위 10개 계통과 짧은 하위 10개 계통을 대상으로 QTL 연관 DNA marker와 호응집성간의 관계분석에서 S4026과 RM287은 gel의 길이와 연관성이 높은 것으로 나타났다.

• The Plant Pathology Journal
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• 제18권5호
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• pp.266-270
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• 2002

Inbred lines of Chinese cabbage KU-101 (resistant line against Plasmodiophora brassicae race race 6) and CS-113 (susceptible line) were crossed and their progeny lines F$_1$, BC$_1$F$_1$, F$_2$, and F$_3$ were produced for the construction of the genetic linkage map of R brassicae race 6-resistant Brassica campestris ssp. pekinensis genome. Restriction fragment length polymorphism (RFLP) was applied to compare between parents and their f$_2$ progenies with a total of 192 probes and 5 restriction enzymes. The constructed RFLP map covered 1,104 cM with a mean distance between genetic marker of 8.0 cM, and produced 10 linkage groups having 121 genetic loci. The loci of P. brassicae race 6 (CR6)-resistant Brassica genome were determined by interval mapping of quan-titative trait loci (QTL), which resulted from bioassay using the same race of the fungi in P3 population. Resistant loci were estimated in numbers 1 (Gl) and 3 (G3) linkage groups. In the regression test, Gl had a value of4.8 logarithm of odd (LOD) score, while C3 had values of 4.2-7.2. Given these results, the location of the CR6-resistant loci within the Brassica genome map can now be addressed.

• Plant Breeding and Biotechnology
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• 제6권4호
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• pp.454-462
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• 2018

Yield and grade are the key factors that affect production value of peanut. The objective of this study was to identify QTLs for pod yield, hundred-seed weight, and total sound mature kernel (TSMK). A total of 90 recombinant inbred lines, derived from Tamrun OL07 and a breeding line Tx964117, were used as a mapping population and planted in Brownfield and Stephenville, Texas. A genetic map was developed using 1,211 SNP markers based on double digest restriction-site associated DNA sequencing (ddRAD-seq). A total of 10 QTLs were identified above the permutation threshold, three for yield, three for hundred-seed weight and four for TSMK, with LOD score values of 3.7 - 6.9 and phenotypic variance explained of 12.2% - 35.9%. Among those, there were several QTLs that were detected in more than one field experiment. The commonly detected QTLs in this study may be used as potential targets for future breeding program to incorporate yield and grade related traits through molecular breeding.

• Asian-Australasian Journal of Animal Sciences
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• 제22권10호
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• pp.1351-1358
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• 2009

Parentage tests using polymorphic DNA marker are commonly performed to avoid incorrect recording of the parental information of livestock animals, and single-nucleotide polymorphisms (SNPs) are becoming the method of choice. In Japanese Black cattle, parentage tests based on the exclusion method using microsatellite markers are currently conducted; however, an alternative SNP system aimed at parentage tests has recently been developed. In the present study, two types of simulations were conducted using the pedigree data of two subpopulations in the breed (subpopulations of Hyogo and Shimane prefectures) in order to examine the effect of actual genetic and breeding structures. The first simulation (simulation 1) investigated the usefulness of SNPs for excluding a close relative of the true sire; the second one (simulation 2) investigated the accuracy of sire identification tests for multiple full-sib putative sires by a combined method of exclusion and paternity assignment based on the LOD score. The success rates of excluding a single fullsib and sire of the true sires were, respectively, 0.9915 and 0.9852 in Hyogo and 0.9848 and 0.9852 in Shimane, when 50 SNPs with minor allele frequency (MAF: q) of 0.25${\leq}$q${\leq}$0.35 were used in simulation 1. The success rates of sire identification tests based solely on the exclusion method were relatively low in simulation 2. However, assuming that 50 SNPs with MAF of 0.25${\leq}$q${\leq}$0.35 or 0.45${\leq}$q${\leq}$0.5 were available, the total success rates including achievements due to paternity assignment were, respectively, 0.9430 and 0.9681 in Hyogo and 0.8999 and 0.9399 for Shimane, even when each true sire was assumed to compete with 50 full-sibs.

• Asian-Australasian Journal of Animal Sciences
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• 제23권1호
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• pp.7-12
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• 2010

The full-length cDNA of the porcine peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase (PECI) gene encodes a monofunctional peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase. Cloning and sequencing of the porcine PECI cDNA revealed the presence of an 1185-base pair open reading frame predicted to encode a 394-amino acid protein by the 5'rapid amplification of cDNA ends (5'RACE) and EST sequences. The porcine PECI gene was expressed in seven tissues (heart, liver, spleen, lung, kidney, skeletal muscle, fat) which was revealed by reverse transcriptase-polymerase chain reaction (RT-PCR). The porcine PECI was mapped to SSC71/2 p11-13 using the somatic cell hybrid panel (SCHP) and the radiation hybrid panel (RH) (LOD score 12.84). The data showed that PECI was closely linked to marker S0383. A C/T single nucleotide polymorphism in PECI exon 10 (3'UTR) was detected as a PvuII PCR-RFLP. Association analysis in our experimental pig population showed that different genotypes of PECI gene were significantly associated with the Average Backfat thickness (ABF) (p<0.05) and Buttock backfat thickness (p<0.01).

• 한국치위생학회지
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• 제16권2호
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• pp.195-204
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• 2016

Objectives: The purpose of the study is to investigate the knowledge, attitude, and recognition of health insurance coverage in tooth implant among Korean adults. Methods: A self-reported questionnaire was completed by 420 adults over 20 years lod in Seoul and Gyeonggido from October 1 to December 15, 2014. The questionnaire consisted of general characteristics of the subjects, knowledge, attitude, and recognition of health insurance coverage in tooth implant. Results: The mean of knowledge of regular checkup after implant was 3.05 points, and mean of advantage and disadvantage of implant was 3.03 points. The expectation for the implant treatment was 4.18 points and this was the highest score. There was no significant difference between the knowledge and attitude in age and monthly compensation(p>0.05). Only 45.6% of the adults were aware of the health insurance coverage of tooth implant since July, 2014. Health Insurance meeds to be modified in the beneficiaries age(44.6%), and cost(32.7%). The positive aspects of implant care included medical expenses(37.8%), health care beneficiary(29.1%), and oral health promotion(20.9%). Conclusions: Health insurance coverage of tooth implant is very important to enhance the quality of life in the adults because the proper management and implementation of the health insurance in implant will improve the oral health care in life.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.281-281
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• 2022

Rice is a staple crop used by more than 50% of the world's population. However, in rapidly changing climates such as abnormal high temperatures and typhoons, the food security of rice is greatly threatened. Plant factories have the advantage of being able to grow crops regardless of climate change, so they can be a response to climate change. However, in plant factories, crops are grown by placing the culture bed vertically, so shorter crops are more efficient. Therefore, in order to search for genes related to the height of rice, QTL analysis was performed by investigating the plant height of Cheongcheong/Nagdong doubled haploids from 2017 to 2021. Plant height of rice investigated for five years showed a normal distribution, meaning that genes related to rice height are quantitative traits. As a result of QTL analysis, a total of 12 QTLs were detected, and QTLs overlapped for 5 years in RM12285-RM212 on chromosome 1. Also, The QTLs of plant height detected in 2019 has a LOD score of 17.64 in RM12285-RM212 region of chromosome 1. As a result of QTL analysis, 44 height-related genes were searched from the detected chromosomes, and among them, Os01g0757200 in RM 12285-RM212 on chromosome 1 region, named OsGA2ox3q1, were selected as genes related to the height of rice. The relative gene expression level of OsGA2ox3q1 was highly expressed in cultivar with short culm lines, and was low expressed in cultivar with long culm lines. OsGA2ox3q1 can be used to breed semi-dwarf cultivar in rice more efficiently.