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http://dx.doi.org/10.13103/JFHS.2016.31.5.311

Establishment of an Analytical Method for Azorubine, an Undesignated Food Colorant in Korea  

Kim, Min-ji (Advanced Food Safety Research group, Brain Korea 21 Plus, School of Food Science and Technology, Chung-Ang University)
Park, Ju-hee (Advanced Food Safety Research group, Brain Korea 21 Plus, School of Food Science and Technology, Chung-Ang University)
Suh, Hee-Jae (Department of Food Science, Sun Moon University)
Lee, Chan (Advanced Food Safety Research group, Brain Korea 21 Plus, School of Food Science and Technology, Chung-Ang University)
Publication Information
Journal of Food Hygiene and Safety / v.31, no.5, 2016 , pp. 311-318 More about this Journal
Abstract
Azorubine is a synthetic tar color containing azo-bond in the molecular structure. This food colorant has been allowed to be used for beverages, cheese and dried fruits in the European Union and for some food in Australia. Even though it is applicable as a food color in many countries, this compound has not been permitted in Korea so far as a food additive. Thus, this study was performed to establish an analysis method for azorubine in Korea by comparison of three HPLC analysis methods for azorubine and other azo-compounds which are officially used in the European Food Safety Authority (EFSA, EU), the Food Standard Agency (FSA, England) and the National Institute of Food and Drug Safety Evaluation (NIFDS, Korea). The analysis method of the FSA for azorubine showed the best linearity ($r^2=0.999$), limit of detection (LOD, $0.07{\mu}g/mL$), limit of quantification (LOQ, $0.20{\mu}g/mL$), precision (0~0.5%) and accuracy (98.6~100.7%) among tested HPLC methods using a C-18 column and diode array detector (DAD) with ammonium acetate solution and acetonitrile as an eluent solution. Finally selected method of FSA was further verified by inter-day and intra-day experiments with linearity, LOD, LOQ, precision and accuracy. Recovery test showed the recover ratios of 97~103%, 95~101%, and 93~102% in beverages, breads/snacks and other foods, respectively. Inter-laboratory test represented the absolute value of z-score of less than 2 which means satisfactory levels in this test. Selected method of FSA showed reliable analytical results in application test using food samples collected in commercial markets in Europe.
Keywords
food colorant; azorubine; E122; analysis; method validation;
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1 Ansari, R., Delavar, A. F., Aliakbar, A., Mohammad-Khah, A.: Solid-state cu (II) ion-selective electrode based on polyaniline-conducting polymer film doped with copper carmoisine dye complex. Journal of Solid State Electrochemistry, 16(3), 869-875 (2012).   DOI
2 Shukla, S. K., Singh, A. K., Murulana, L. C., Kabanda, M. M., Ebenso, E. E.: Inhibitive effect of azorubine dye on the corrosion of mild steel in hydrochloric acid medium and synergistic iodide additive. International Journal of Electrochemical Science, 7, 5057-5068 (2012).
3 Haigh, R.: Harmonization of legislation on foodstuffs, food additives and contaminants. International Journal of Food Science & Technology, 13(6), 491-509 (1978).   DOI
4 Gupta, V. K., Mittal, A., Malviya, A., Mittal, J.: Adsorption of carmoisine A from wastewater using waste materials-bottom ash and deoiled soya. Journal of Colloid and Interface Science, 335(1), 24-33 (2009).   DOI
5 Smith, J., Hong-Shum, L.: Food additives data book, 2nd Ed. Wiley-backwell, pp. 196-198 (2011).
6 Snehalatha, M., Ravikumar, C., Joe, I. H., Sekar, N., Jayakumar, V.: Spectroscopic analysis and DFT calculations of a food additive carmoisine. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 72(3), 654-662 (2009).   DOI
7 Arocas, A., Varela, P., Gonzalez-Miret, M. L., Salvador, A., Heredia, F., Fiszman, S.: Differences in colour gamut obtained with three synthetic red food colourants compared with three natural ones: PH and heat stability. International Journal of Food Properties, 16(4), 766-777 (2013).   DOI
8 European Commission.: European Parliament and Council Directive 94/36/EC. Official Journal of the European Communities, No. L 237/13 (1994).
9 JECFA: Evaluation of certain food additives and contaminants. World Health Organization Technical Report Series 696, (1983).
10 FDA.: Food and Drug Administration, Compliance Program Guidance Manual, Chapter 03 - Foodborne Biological Hazards. 37 (2008).
11 Bonan, S., Fedrizzi, G., Menotta, S., Elisabetta, C.: Simultaneous determination of synthetic dyes in foodstuffs and beverages by high-performance liquid chromatography coupled with diode-array detector. Dyes and Pigments, 99(1), 36-40 (2013).   DOI
12 FDA.: Background Document for the Food Advisory Committee: Certified Color Additives in Food and Possible Association with Attention Deficit Hyperactivity Disorder in Children, pp. 1-13 (2011).
13 Amin, K., Hameid, H. A., Elsttar, A. A.: Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food and Chemical Toxicology, 48(10), 2994-2999 (2010).   DOI
14 McCann, D., Barrett, A., Cooper, A., Crumpler, D., Dalen, L., Grimshaw, K., Kitchin, E., Lok, K., Porteous, L., Prince, E., Sonuga-Barke, E., O Warner, J., Stevenson, J.: Food additives and hyperactive behaviour in 3-year-old and 8/9-year-old children in the community: A randomised, double-blinded, placebo-controlled trial. The Lancet, 370(9598), 1560-1567 (2007).   DOI
15 Larsen, J. C., Mortensen, A., Hallas-Moller, T. Scientific Opinion on the Re-Evaluation of Azorubine/Carmoisine (E 122) as a Food Additive. EFSA Journal, 7(11), 1332-1372 (2009).   DOI
16 FSA.: Survey of colours in sweets. FSA Imformation Sheets, No. 23/02, April, (2002).
17 Serdar, M., Kne evi , Z.: Simultaneous LC analysis of food dyes in soft drinks. Chromatographia, 70(9-10), 1519-1521 (2009).   DOI
18 National Institute of Food and Drug Safety Evaluation.: Food additives analysis methods. National Institute of Food and Drug Safety Evaluation, Chung-ju, pp. 12-9-1-12-9-2 (2011).
19 Park, S.K., Lee, T.S., Park, S.K.: Method development for the sample preparation and quantitative analysis of synthetic colors in foods. Korean J. Food SCI TECHNOL., 36(6), 893-899 (2004).
20 Minioti, K. S., Sakellariou, C. F., Thomaidis, N. S.: Determination of 13 synthetic food colorants in water-soluble foods by reversed-phase high-performance liquid chromatography coupled with diode-array detector. Analytica Chimica Acta, 583(1), 103-110 (2007).   DOI
21 Sistla, R., Tata, V., Kashyap, Y., Chandrasekar, D., Diwan, P. Development and validation of a reversed-phase HPLC method for the determination of ezetimibe in pharmaceutical dosage forms. Journal of Pharmaceutical and Biomedical Analysis, 39(3), 517-522 (2005).   DOI
22 Feng, F., Zhao, Y., Yong, W., Sun, L., Jiang, G., Chu, X.: Highly sensitive and accurate screening of 40 dyes in soft drinks by liquid chromatography-electrospray tandem mass spectrometry. Journal of Chromatography B, 879(20), 1813-1818 (2011).   DOI
23 ICH Expert Working Group : Validation of analytical procedures: Text and methodology. Q2 (r1), 1 (2005).
24 Eng C. F., Dra R. A., Rui L.: Interlaboratory Comparison of Analytical Results a Measure of Quality Control. The Micro Report, 14(1), 4-7 (2003).
25 Yoshioka, N., Ichihashi, K.: Determination of 40 synthetic food colors in drinks and candies by high-performance liquid chromatography using a short column with photodiode array detection. Talanta, 74(5), 1408-1413 (2008).   DOI
26 ISO: Accuracy (Trueness and Precision) of Measurement Methods and Results-Part, 1: Introduction and basic principles, (2011).
27 Hund, E., Massart, D. L., Smeyers-Verbeke, J.: Inter-laboratory studies in analytical chemistry. Analytica Chimica Acta, 423(2), 145-165 (2000).   DOI
28 Fortuna, T., Ga kowska, D., Socha, R., Juszczak, L.: Red synthetic dyes in traditional sweets. Potravinarstvo: Scientific Journal for Food Industry, 7(Special Issue), 33-36 (2013).
29 Vlase, L., Muntean, D., Cobzac, S. C., Filip, L.: Development and validation of an hplc-uv method for determination of synthetic food colorants. Rev. Roum. Chim, 59(9), 719-725 (2014).
30 Brittain, H.: Valida ao de metodos analíticos nao cromatograficos. Pharm. Technol., Ed. Bras, 4-9 (1998).