• Title/Summary/Keyword: LOCUS

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Molecular Marker Analysis for Resistance of Soybean Cultivars to Soybean Cyst Nematode

  • Chung, Jong-Il;Park, Won-Gyeong;Park, Min-Jung;Ko, Mi-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.4
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    • pp.319-322
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    • 2002
  • Soybean cyst nematode (Heterodera glycines Ichinohe; SCN) is an important soybean pest and the use of resistant cultivars is the effective method to reduce or eliminate SCN damage. However, breeding for SCN resistance is difficult and expensive by the oligogenic nature of the resistance and genetic variability in the pathogen. Fortunately, SCN resistance loci, rhg1 and Rhg4 are generally accepted as a necessity for the development of resistant genotypes using any source of resistance. In this study, resistance of 44 Korean soybean cultivars to SCN was tested using two molecular markers. Seonheukkong and Pokwangkong were the homozygous to rhg1 locus. Seven cultivars were susceptible to SCN based on Satt309 marker linked rhg1 locus. All Korean cultivars estimated in this study were recessive homozygous to Rhg4 locus and were susceptible in the PCR reaction using primer 548/563 linked to the Rhg4 locus conferring resistance to SCN race 3. Among 44 cultivars estimated, seven cultivars were susceptible to SCN in both Satt309 and primer 548/563 markers. Based on both Satt309 and primer 548/563 markers, there is no resistant cultivar to SCN in Korea. Therefore, SCN resistant cultivars need to be developed in the future. These two markers can be used for improving SCN resistant cultivars.

Health Locus of Control and Health-promoting Behaviors among Korean Elementary School Children (학령기 아동의 비만도, 체중조절 건강통제위와 건강증진행위)

  • Jang, Ji-Yeon;Bang, Kyung-Sook
    • Korean Parent-Child Health Journal
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    • v.14 no.1
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    • pp.18-27
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    • 2011
  • Purpose: The purpose of this study was to compare health locus of control with health-promoting behaviors according to elementary school children's degree of obesity, and to analyze correlations between two variables. Methods: Three hundreds and sixty three elementary school children were participated from three elementary schools in Seoul. Multidimensional Health Locus of Control (HLOC) scale and Health Promoting Lifestyle Profile (HPLP) were used. Results: Obese children was 16.8% of all research subjects. Internal HLOC in over weight group was higher than normal weight group (F=3.611, p=.014). Chance HLOC in under weight group was higher than other groups (F=3.553, p=.015). External HLOC in over weight and obesity group was higher than normal weight group (F=3.553, p=.015). Correlations between HLOC in internal and external and health-promoting behaviors were significant (r=.347, p<001; r=.207, p<001). Also, children who did not have siblings and have obese parents showed higher rate of obesity, and lack of sleeping time tend to be related to obesity. Conclusion: External HLOC of obesity children is higher than that of normal weight children. Meanwhile, obese children with higher internal HLOC were good at doing health-promoting behaviors. Therefore, if obese children are trained for internal health control, it can lead to their health-promoting behaviors.

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Creative Problem Solving Style, Self-leadership and Locus of Control in the Korean Engineering College Students (공대생들의 창의적 문제해결유형별 셀프리더십과 통제소재)

  • Ahn, Jeong-Ho;Lim, Jee-Young
    • Journal of Engineering Education Research
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    • v.13 no.4
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    • pp.122-129
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    • 2010
  • This study was conducted to compare the self-leadership strategies and locus of control between the problem-solving styles in the Korean engineering college students. Creative problem-solving styles were identified based on the three dimensions. The results of the two-way analysis of variances indicated the main effects of problem-solving styles; each problem-solving style had unique characteristics in relation to self-leadership strategies and locus of control. There were neither main effects of sex nor interaction effects of problem-solving styles and sex. It would be useful to provide the engineering students with the specialized self-leadership training program based on the problem-solving styles.

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Microsatellite analysis of 20 mulberry varieties preserved in Korea

  • Chan Young, Jeong;Sang Kuk, Kang;Nam-Suk, Kim;Ik Seob, Cha;Seong-Wan, Kim;Jong Woo, Park;Kee-Young, Kim
    • International Journal of Industrial Entomology and Biomaterials
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    • v.45 no.2
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    • pp.49-55
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    • 2022
  • A total of 20 mulberry varieties preserved in Korea were typed for eight polymorphic microsatellite loci. We obtained 6 to 15 alleles per locus with an average value of 10.6, per-locus observed heterozygosity ranging from 0.35 to 1.00, and per-locus polymorphic information content (PIC) ranging from 0.61 to 0.87, indicating that most loci are highly variable. Phylogenetic analysis using the eight microsatellite loci was sufficiently suitable for classifying 20 mulberry varieties preserved in Korea. A total of 160 variety-specific apomorphic alleles were obtained from eight loci discriminated 20 mulberry varieties. These variety-specific alleles from this analysis are expected to be useful for the discrimination of other mulberry varieties. Furthermore, a substantial number of homozygote loci, represented by 60 among 180 alleles in eight loci were found. These results collectively suggest that these microsatellite locus primers are potentially crucial molecular markers for the eventual classification of mulberry varieties that are preserved as hundreds in Korea.

A Case of Single-step Mutations at Two Short Tandem Repeat loci (D13S317 and DXS10148) among Three Generations of a Korean Family

  • Byeong Ju Youn;Kyungmyung Lee;Cho Hee Kim
    • Biomedical Science Letters
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    • v.28 no.4
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    • pp.327-333
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    • 2022
  • The DNA profiling of short tandem repeat (STR) markers is a powerful tool for forensic identification and forensic paternity testing. However, STR loci are susceptible to mutation that cause mismatches between parents and children when paternity is tested. Herein, we examined paternity disputes with 23 autosomal STR loci using two commercial human identification kits and revealed successive mismatches at the D13S317 locus across three generations of a Korean family. Additionally, we investigated 12 X-chromosomal STRs and discovered an inconsistency at the DXS10148 locus between the father and daughter of the same Korean family. Furthermore, we confirmed STR genotypes at the D13S317 and DXS10148 loci of the family using sequencing analysis. Consequently, we identified a successive single-step mutation at the D13S317 locus and one single-step mutation at the DXS10148 locus in three generations of the Korean family. Therefore, this case study may be useful for interpreting and understanding forensic paternity tests.

The influence of the locus of control of middle aged on life satisfaction and preparation for old age among in the times of convergence (융복합시대 중년기성인의 내외통제성이 생활만족도 및 노후준비에 미치는 영향)

  • Lee, Hee-Youn;Jeon, Hye-Seong
    • Journal of Digital Convergence
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    • v.13 no.11
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    • pp.433-440
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    • 2015
  • This paper was to explore the effect of the locus of control on life satisfaction and preparation for old age among middle-aged adults in the time of Convergence. This survey was conducted from September to December 2013, and 284responses were used for the analysis. The data was analyzed by descriptive statistics, Pearson Correlation and Multiple Regression. The research findings could be summarized as the followings. First, the locus of control had significantly positive effect on life satisfaction, Second, the locus of control also had significant effect on preparation for old age among middle-aged adults. Third, life satisfaction between preparation for old age had positive correlations with life satisfaction.

Microsatellite Analysis of the Genetic Diversity and Population Structure in Dairy Goats in Thailand

  • Seilsuth, Somkiat;Seo, Joo Hee;Kong, Hong Sik;Jeon, Gwang Joo
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.3
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    • pp.327-332
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    • 2016
  • The genetic relationships between different populations and breeds of exotic dairy goats in Thailand were studied using 12 microsatellite markers. Blood samples were obtained from 211 goats from Department of Livestock Development breeding and research farms: 29 Anglonubian (AN), 21 Alpine (AP), 23 Jamunapari (JAM), 50 Saanen (SN), and 88 Toggenburg (TG). Five of the 12 microsatellite markers were found to be polymorphic. A mean of 7.40 alleles per locus was found, with a range from 5 (SPS115 and ETH225) to 11 (TGLA122). We found 24, 27, 19, 32, and 24 alleles in the AN, AP, JAM, SN, and TG breeds, respectively; 37 alleles were present in all breeds. The mean number of alleles in each population ranged from 3.2 (ETH225 locus) to 7.6 (TGLA122 locus). Genetic variability within the breeds was moderate as evidenced by the mean expected heterozygosity of 0.539. The average observed heterozygosity across the 5 markers in all breeds was 0.529 with the maximum observed at the BM1818 locus (0.772) and the minimum at the ETH225 locus (0.248). The observed and expected heterozygosity for all breeds for the 5 microsatellite markers ranged from 0.419 to 0.772 and 0.227 to 0.792, respectively. On the basis of their means, the TGLA122 and BM1818 loci were the most suitable markers for distinguishing genetic diversity among the goats. The estimated average $F_{is}$ value for the breeds ranged from -0.044 (ETH225) to 0.180 (SPS115), while the estimated average $F_{st}$ value ranged from 0.021 (SPS115) to 0.104 (ETH10). These results indicated that TGLA122 and BM1818 markers are suitable to be used for aiding conservation and breeding improvement strategies of dairy.

A study of Factors Influencing Health Promoting Behavior in College Students (대학생의 건강증진행위에 영향을 미치는 요인에 관한 연구)

  • Paek, Kyung-Shin
    • Research in Community and Public Health Nursing
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    • v.14 no.2
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    • pp.232-241
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    • 2003
  • Purpose: To investigate the factors influencing health promoting behavior in college students and to provide a basic data for developing an effective health promotion program. Method: The subjects were 711 college students living in Jecheon city and were selected using a convenience sampling method. The instruments used in this study included the Health Promoting Lifestyle Profile developed by Walker et al.(987). Perceived Health Status developed by Lawston et al. (1982). Self-Esteem scale developed by Rosenberg(1965), Self-Efficacy scale developed by Becker et al. (1993), and Health Locus of Control developed by Wallston et a1.(1978). The data were analyzed by descriptive statistics, pearson correlation coefficient, and stepwise multiple regression using SPSS/WIN program. Results: I) The mean score of health promoting behavior was 2.39 point out of 4. In terms of sub-domains of health promoting behavior, self-actualization(2.78) showed the highest mean score, followed by interpersonal support(2.75), stress management(2.38), nutrition(2.11), exercise(2.04), and health responsibility (1.97). 2) The health promoting behavior had significantly positive correlations with self-efficacy, powerful others health locus of control, internal health locus of control, chance health locus of control, and perceived health status. 3) In the relationship between general characteristics and health promoting behavior, health promoting behavior was significantly different by gender(t=2.17, p=.03), and financial status of parents (F=10.79. p= .00). 4) The most powerful predictor of health promoting behavior was self-efficacy. A combination of self-efficacy, self-esteem, powerful others health locus of control, and sex accounted for 40.4% of the total variance in health promoting behavior. Conclusion: The findings of this study showed that health responsibility and exercise were the domains where the college student showed relatively lower scores than other domains, self-efficacy was the most important predictor of health promoting behavior. Therefore, it is suggested that health promoting programs should focus on health responsibility, and exercise. Nursing strategies that can enhance self-efficacy should also be developed in order to promote healthy lifestyles in college students.

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A study on the life style. locus of control and health belief of gastric cancer patients (위암환자의 생활양식, 건강신념 및 건강 통제위)

  • So, Hee-Young;Kim, Hyun-Il
    • Research in Community and Public Health Nursing
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    • v.10 no.2
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    • pp.362-371
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    • 1999
  • This study was conducted to explore the relationship between locus of control and health belief. the life style of gastric cancer patients. The subjects of this study were 40 of the early gastric cancer and 90 of the advanced gastric cancer who first visiting patient to general surgery out patient department of Chungnam University Hospital for operation. The data was collected with structured questionnaire from July. 1998 to Feb. 1999 The tool were Moon's Health Belief Scale and Multiple Locus of Control of Wallston. Wallston, DeVellis. The data was analysed by SAS program using frequency, $X^2$-test, Pearson's correlation coefficient, ANOVA. Scheffe-test, t-test. The results were as follows 1. 30.8% of subjects were early gastric cancer anf 69.2% were advanced gastric cancer. The subject knowing about diagnosis was 83.1%. 16.9% did'nt know about diagnosis. 2. The correlationship between Health belief and Locus of control of subjects was not supported. 3. There was statistically no difference of life style between early and advanced gastric cancer patients. 4. There were statistically significant differences in perceived sensitivity according to weight. educational level. and birth order, in perceived barrier according to educational level. and in perceived severity according to occupation among demographic characteristics of study subjects. From above results. health professional has to educate general population to detect gastric cancer early to improve survival rate because early gastric cancer is high in survival rate, and to prevent recurrence and to maintain continuing healthy status. In the future, gastroscopy also has to expand to detect early. For there was no difference in life style between early and advanced gastric cancer. carcinogen related to diet should be emphasized through education. The perceived benefit among health belief model was not supported in this study therefore further study and comparison between gastric cancer and normal population are needed.

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A Comparative Study on the Relationships of Depression, Fatigue, BMI, and Health Locus of Control between Urban and Rural Female Middle School Students (도시 농촌 간 여중생의 우울, 피로, 체질량지수, 건강통제위 비교)

  • Choi, Hye-Jung
    • Journal of Korean Public Health Nursing
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    • v.22 no.2
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    • pp.224-236
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    • 2008
  • Purpose: The principal objective of this study was to assess the relationships of depression, fatigue, BMI, and health locus of control between urban and rural female middle school students. Methods: The study subjects included 377 girls in randomly-sampled middle schools in urban and rural provinces. The data were collected via self-recorded questionnaires from April 21.- to May 2. 2008, and analyzed using the SPSS Win 12.0 program. Results; 1) The mean score of depression was $10.22{\pm}5.72$ (urban), $10.81{\pm}4.92$ (rural). 2) The mean fatigue score was $50.43{\pm}1.57$ (urban), $51.90{\pm}9.70$ (rural). 3) BMI was classified into 3 groups: leptosomic, normal weight, and obesity groups. The mean BMI score was $18.73{\pm}2.05$ (Seoul), and $20.31{\pm}3.26$ (provincial). 4) The internal locus of control was higher than the external locus of control (urban). 5) Fatigue was correlated with depression (r=.512, p=.000) in both areas. The health locus of control was correlated with Fatigue (r=.153, p=.042) in province. Conclusion: An effective program to reduce the levels of fatigue and depression for middle school girls and to maintain normal weight is required. In particular, the high-risk depression group can be managed through community mental health care center deep interviews, and close observation.

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