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http://dx.doi.org/10.7852/ijie.2022.45.2.49

Microsatellite analysis of 20 mulberry varieties preserved in Korea  

Chan Young, Jeong (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Sang Kuk, Kang (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Nam-Suk, Kim (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Ik Seob, Cha (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Seong-Wan, Kim (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Jong Woo, Park (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Kee-Young, Kim (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.45, no.2, 2022 , pp. 49-55 More about this Journal
Abstract
A total of 20 mulberry varieties preserved in Korea were typed for eight polymorphic microsatellite loci. We obtained 6 to 15 alleles per locus with an average value of 10.6, per-locus observed heterozygosity ranging from 0.35 to 1.00, and per-locus polymorphic information content (PIC) ranging from 0.61 to 0.87, indicating that most loci are highly variable. Phylogenetic analysis using the eight microsatellite loci was sufficiently suitable for classifying 20 mulberry varieties preserved in Korea. A total of 160 variety-specific apomorphic alleles were obtained from eight loci discriminated 20 mulberry varieties. These variety-specific alleles from this analysis are expected to be useful for the discrimination of other mulberry varieties. Furthermore, a substantial number of homozygote loci, represented by 60 among 180 alleles in eight loci were found. These results collectively suggest that these microsatellite locus primers are potentially crucial molecular markers for the eventual classification of mulberry varieties that are preserved as hundreds in Korea.
Keywords
Mulberry; Microsatellite DNA; Allele; Simple sequence repeat;
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