• 제목/요약/키워드: LMV

검색결과 13건 처리시간 0.02초

Characteristics of a Lettuce mosaic virus Isolate Infecting Lettuce in Korea

  • Lim, Seungmo;Zhao, Fumei;Yoo, Ran Hee;Igori, Davaajargal;Lee, Su-Heon;Lim, Hyoun-Sub;Moon, Jae Sun
    • The Plant Pathology Journal
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    • 제30권2호
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    • pp.183-187
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    • 2014
  • Lettuce mosaic virus (LMV) causes disease of plants in the family Asteraceae, especially lettuce crops. LMV isolates have previously been clustered in three main groups, LMV-Yar, LMV-Greek and LMV-RoW. The first two groups, LMV-Yar and LMV-Greek, have similar characteristics such as no seed-borne transmission and non-resistance-breaking. The latter one, LMV-RoW, comprising a large percentage of the LMV isolates contains two large subgroups, LMV-Common and LMV-Most. To date, however, no Korean LMV isolate has been classified and characterized. In this study, LMV-Muju, the Korean LMV isolate, was isolated from lettuce showing pale green and mottle symptoms, and its complete genome sequence was determined. Classification method of LMV isolates based on nucleotide sequence divergence of the NIb-CP junction showed that LMV-Muju was categorized as LMV-Common. LMV-Muju was more similar to LMV-O (LMV-Common subgroup) than to LMV-E (LMV-RoW group but not LMV-Common subgroup) even in the amino acid domains of HC-Pro associated with pathogenicity, and in the CI and VPg regions related to ability to overcome resistance. Taken together, LMV-Muju belongs to the LMV-Common subgroup, and is expected to be a seed-borne, non-resistance-breaking isolate. According to our analysis, all other LMV isolates not previously assigned to a subgroup were also included in the LMV-RoW group.

Spatial Characteristics of Low Meteorological Visibility over Hongkong and Statistical Retrieval from Satellite Data

  • Fei, HUANG;Jun-Ping, QIAN;Zu-Qiang, CUI;Zhi-Hong, ZHENG;Zhi-Jun, WU
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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    • pp.1261-1263
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    • 2003
  • Based on twelve observational stations low meteorological visibility (LMV) data during November 2002 to April 2003, the spatial distribution of LMV over Hongkong area (113.8$^{\circ}$ E-114.4$^{\circ}$ E, 22.1$^{\circ}$ N-22.4$^{\circ}$ N) is studied, using a PCA method. Optical spectrum of NOAA-16 associated with LMV shows that the significant effect factors correlated with LMV in the leading mode are the difference or rate between the visible and near-IR channels and single visible channel. A successful retrieval of LMV is done and a regression equation with a multiple correlation coefficient of 0.67 is obtained.

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십자화과 작물 종자에서 종자전염 세균 및 바이러스 동시 검출을 위한 One-step Multiplex RT-PCR 방법 (One-step Multiplex RT-PCR Method for Simultaneous Detection of Seed Transmissible Bacterium and Virus Occurring on Brassicaceae Crop Seeds)

  • 정규식;소은희
    • 식물병연구
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    • 제17권1호
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    • pp.52-58
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    • 2011
  • 우리나라에서 주로 재배되는 십자화과 작물(상추, 콜라비, 무, 배추, 양배추)의 종자 전염 병원균 중에서 세균성 병원균 Xanthomonns campestris pv. campestris(Xcc)와 바이러스 병원균 Lettuce Mosaic Virus(LMV)를 종자에서 동시검출하기 위한 One-step multiplex RT-PCR을 개발하였다. 각각의 병원균을 특이적으로 증폭시키는 병원균 검출용 프라이머 2종(Xcc-F/R, LMV-F/R)을 primerblast 프로그램을 이용하여 제작하였고 이들 프라이머 세트는 프라이머간 또는 병원균 cDNA간의 간섭없이 특이적으로 타겟 병원균만을 검출하였다. PCR을 이용한 병원균의 검출 최소 민감도는 1 ng이었다. 십자화과 작물중에서 유통 중인 콜라비 10품종, 상추 50품종, 무 20품종, 배추 20품종 그리고 양배추 20품종에 대한 종자 감염 병원균 검출을 위한 One-step multiplex RT-PCR 수행 결과, LMV는 전체 120품종 중에서 39품종에서 검출되었고, Xcc는 2개 품종에서 검출되었다. 그리고 50품종의 상추 종자 시료 중에 8품종의 시료에서 LMV와 Xcc가 동시 검출되었다.

Planarian 모델을 이용한 억간산의 항발작 효과 (Effects of Ukgansan (Yokukansan in Japanese, Yigansan in chinese) on the Locomotor Velocity and Glutamate-Induce Paroxysm in Planarian)

  • 박웅;유두만;소준노
    • KSBB Journal
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    • 제29권1호
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    • pp.67-71
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    • 2014
  • Planaria were recently reported to be a simple and sensitive model to investigate the mechanistic aspect of seizure and to screen potential anticonvulsants. Using planarian model, we analyzed the pharmacological effect of ukgansan (UGS), an oriental herbal medicine containing seven medicinal herbs, on the planarian locomotor velocity (pLMV) and glutamate-induced seizure-like activity (pSLA). To test whether D. japonica is suitable for studying anti-seizure agents, we investigated the effect of glutamate on pLMV and pSLA in D. japonica. In the present study we first confirmed that pSLA in D. japonica was induced by L-glutamate. Glutamate significantly produced pSLA in a dose dependent manner, but did not affect pLMV. These glutamate-induced paroxysms were decreased by antiepileptic drug, topiramate. A similar inhibitory effect on glutamate-induced pSLA was observed after the treatment of UGS. The present results suggest that UGS and its active constituents possess useful substance inhibiting seizure in planarian and that D. japonica provides a convenient model to search active herbs containing anti-seizure activity.

실험 계획법을 이용한 진공 차단기의 동특성 최적화 (Dynamic Responses Optimization of Vacuum Circuit Breaker Using Taghchi Method)

  • 조준연;안길영;김성태;양홍익;김규정
    • 대한기계학회논문집 C: 기술과 교육
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    • 제3권2호
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    • pp.141-148
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    • 2015
  • 본 연구에서는 진공 차단기(Vacuum circuit breaker)의 동적 특성을 모사하기 위해 리커다인을 이용한 다물체 동역학 해석 모델이 개발 되었다. 진공 차단기는 VI(Vacuum interrupter)를 포함한 3 개의 주회로 부와, 구동 메커니즘을 포함한 기구부로 구성된다. 이 해석적 모델의 검증을 위해, 해석 결과와 실측을 통한 실험적 결과를 비교 하였다. 일반적으로, 원활한 사고 전류 차단을 위해서는 0.9~1.1m/s 의 차단속도(Opening velocity)가 필요하다. 차단 속도의 향상을 위해, 다구찌 기법을 이용하여 진공 차단기의 설계 변수 최적화를 수행하였다. 또한 향상된 차단기의 동적 특성을 검증하기 위해, 해석 결과와 개선된 샘플의 실험적 결과를 비교 분석 하였다.

Quadrature VCO as a Subharmonic Mixer

  • Oh, Nam-Jin
    • International journal of advanced smart convergence
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    • 제10권3호
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    • pp.81-88
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    • 2021
  • This paper proposes two types of subharmonic RF receiver front-end (called LMV) where, in a single stage, quadrature voltage-controlled oscillator (QVCO) is stacked on top of a low noise amplifier. Since the QVCO itself plays the role of the single-balanced subharmonic mixer with the dc current reuse technique by stacking, the proposed topology can remove the RF mixer component in the RF front-end and thus reduce the chip size and the power consumption. Another advantage of the proposed topologies is that many challenges of the direct conversion receiver can be easily evaded with the subharmonic mixing in the QVCO itself. The intermediate frequency signal can be directly extracted at the center taps of the two inductors of the QVCO. Using a 65 nm complementary metal oxide semiconductor (CMOS) technology, the proposed subharmonic RF front-ends are designed. Oscillating at around 2.4 GHz band, the proposed subharmonic LMVs are compared in terms of phase noise, voltage conversion gain and double sideband noise figure. The subharmonic LMVs consume about 330 ㎼ dc power from a 1-V supply.

A Decomposition Algorithm for a Local Access Telecommunication Network Design Problem

  • Cho, Geon
    • 한국경영과학회지
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    • 제23권2호
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    • pp.29-46
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    • 1998
  • In this paper, we develop detailed algorithms for implementing the so-called Limited Column Generation procedure for Local Access Telecommunication Network(LATN) design problem. We formulate the problem into a tree-partitioning problem with an exponential number of variables. Its linear programming relaxation has all integral vertices, and can be solved by the Limited Column Generation procedure in just n pivots, where n is the number of nodes in the network. Prior to each pivot. an entering variable is selected by detecting the Locally Most Violated(LMV) reduced cost, which can be obtained by solving a subproblem in pseudo-polynomial time. A critical step in the Limited Column Generation is to find all the LMV reduced costs. As dual variables are updated at each pivot, the reduced costs have to be computed in an on-line fashion. An efficient implementation is developed to execute such a task so that the LATN design problem can be solved in O(n$^2$H), where H is the maximum concentrator capacity. Our computational experiments indicate that our algorithm delivers an outstanding performance. For instance, the LATN design problem with n=150 and H=1000 can be solved in approximately 67 seconds on a SUN SPARC 1000 workstation.

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A decomposition algorithm for local access telecommunication network design problem

  • Cho, Geon
    • 한국경영과학회:학술대회논문집
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    • 대한산업공학회/한국경영과학회 1995년도 춘계공동학술대회논문집; 전남대학교; 28-29 Apr. 1995
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    • pp.54-68
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    • 1995
  • In this paper, we develop detailed algorithms for implementing the so-called Limited Column Generation procedure for Local Access Telecommunication Network (LATN) Design problem. We formulate the problem into a tree-partitioning problem with an exponential number of variables. Its linear programming relaxation has all integral vertices, and can be solved by the Limited Column. Generation procedure in just n pivots, where n is the number of nodes in the network. Prior to each pivot, an entering variable is selected by detecting the Locally Most Violated (LMV) reduced cost, which can be obtained by solving a subproblem in pseudo-polynomial time. A critical step in the Limited Column Generation is to find all the LMV reduced costs. As dual variables are updated at each pivot, the reduced costs have to be computed in an on-line fashion. An efficient implementation is developed to execute such a task so that the LATN Design problem can be solved in O(n$^{2}$H), where H is the maximum concentrator capacity. Our computational experiments indicate that our algorithm delivers an outstanding performance. For instance, the LATN Design problem with n = 150 and H = 1000 can be solved in approximately 67 seconds on a SUN SPARC 1000 workstation.

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Single-balanced Direct Conversion Quadrature Receiver with Self-oscillating LMV

  • Nam-Jin Oh
    • International Journal of Internet, Broadcasting and Communication
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    • 제15권3호
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    • pp.122-128
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    • 2023
  • This paper proposes two kinds of single-balanced direct conversion quadrature receivers using selfoscillating LMVs in which the voltage-controlled oscillator (VCO) itself operates as a mixer while generating an oscillation. The two LMVs are complementary coupled and series coupled to generate the quadrature oscillating signals, respectively. Using a 65 nm CMOS technology, the proposed quadrature receivers are designed and simulated. Oscillating at around 2.4 GHz frequency, the complementary coupled quadrature receiver achieves the phase noise of -28 dBc/Hz at 1KHz offset and -109 dBc/Hz at 1 MHz offset frequency. The other series coupled receiver achieves the phase noise of -31 dBc/Hz at 1KHz offset and -109 dBc/Hz at 1 MHz offset frequency. The simulated voltage conversion gain of the two single-balanced receivers is 37 dB and 45 dB, respectively. The double-sideband noise figure of the two receivers is 5.3 dB at 1 MHz offset. The quadrature receivers consume about 440 μW dc power from a 1.0-V supply.

만성 B형간질환에서 HBV백신 및 항바이러스치료가 간세포암종 발생에 미치는 효과 (Long Term Effects of Lamivudine and Adefovir dipivoxil in Chronic Hepatitis B Patients on the Development of Hepatocellular Carcinoma)

  • 이헌주
    • Journal of Yeungnam Medical Science
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    • 제25권1호
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    • pp.1-18
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    • 2008
  • 1980년대부터 시행된 HBV 감염의 예방 백신접종으로 HBV 감염율이 감소되고 1997년부터 시작된 핵산유사체인 LMV의 항바이러스치료 효과로 인하여 CHB 환자에서 HBV 증식 억압이 가능하게 되면서 10~20여년 동안 HCC 발생율이 현저히 감소된다는 보고가 증가하고 있으며 21세기 전반기에는 1980년대 부터인 백신접종가능 시기 이후의 출생자들이 성인이 되는 시점이 되면 HBV 감염율 감소로 동반된 HCC 발생율의 현저한 감소를 예측하고 있다. 이미 백신 개발 이 전에 또는 백신접종의 기회를 얻지 못하고 이미 영유아 시기에 감염되어 CHB에 이환된 환자들의 간경변증, HCC 등 만성 합병증을 치료하기 위해서는 적극적인 항바이러스치료가 필요하였으나 1980년대 후반에 유일하게 CHB 치료제로 인정된 인터페론은 치료 적응증이나 그 효과에 있었으나 극소수의 제한된 환자에서 제한된 효과만이 입증되었으며 특히 역학적 차이로 인한 바이러스학적, 개체 면역학적 조건에 따라 전혀 고려되지 않는 경우가 많고 수직감염자가 대다수인 우리나라를 포함한 대부분의 고감역지역에서는 우선 선택되지 않는 약제였으며 장기 효과에 대해서는 이십여년이 지난 시점에도 확실하지 않은 상황에서, 1997년부터 극적인 항바이러스 효과를 나타내는 LMV을 투약할 수 있게 되었다. LMV의 HCC 발생 억제효과를 높이기 위해 개선해야 할 점은 필수적인 장기투약에 의해 발생되는 내성 바이러스의 출현이며 이는 간기능뿐 만아니라 HCC 발생의 위험도 높일 수 있다는 보고도 있다. LMV 이후 처방 허가된 ADV, telbivudine(LdT), ETV, CLV, TDF 등도 장기 투약시 내성바이러스 발생이 가능하나 내성 발생을 줄이기 위해서는 적절한 약의 선택과 새로운 약제의 개발과 동시에 치료 원칙의 정립이 필요하다. 그러나 이러한 문제점이 있음에도 불구하고 항바이러스치료로 CHB의 진행 및 만성 합병증 발생의 빈도가 감소되고 있으며 HCC 발생 역시 감소될 것이며, 그 효과를 더 높이기 위해서는 내성변이종 발생 문제를 해결해야 하며 치료대상, 치료기준, 약제의 선택과 방법 및 면밀한 검사관리가 필요하다. 그러나 무엇보다 중요한 것은 일단 HBV에 감염되면 과거감염이건 현재 감염이건 현재의 핵산유사체 제제로 HBV DNA 증식을 억제하더라도 간조직내 cccDNA와 융합된 HBV DNA를 보유할 수 있으며 그 증거로는 HBsAg이 음성인 HCC 환자의 혈청이나 간조직에서 HBV DNA가 발견되는 빈도가 높다는 증거가 무수히 많고, 상황에 따라서는 간질환의 원인이 된다는 보고가 다수 있으므로 가장 완벽하고 확실한 예방법인 HBV 백신 접종이 무엇보다 필수적이며 전세계 대부분의 HCC 발생을 예방할 수 있다. 또한 현재 제시되고 있는 항바이러스제 치료기준은 혈청 간세포괴사치 상승을 조건으로 제한하는데 혈청 ALT치나 HBeAg 상태에 무관하게 간 조직의 상태나 HBV DNA치의 증가와 비례해서 간경변이나 HCC 발생의 위험이 높아진다는 사실이 중요하다.

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