• Title/Summary/Keyword: LMG

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소금의 종류에 따른 새우젓의 이화학적 특성

  • 김미리;오상희
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.04a
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    • pp.108.1-108
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    • 2003
  • 새우젓은 새우와 소금만을 원료로 하여 제조하는데, 최근 수입산 소금의 유입이 급증하고 있어 새우젓에도 이들 수입산 소금의 사용이 늘어가고 있다. 수입산 소금은 수분과 식염함량이 국산 소금과 다를 뿐 아니라 불순물과 무기질 등의 성분 또한 차이가 있다. 본 연구는 소금의 종류가 새우젓에 어떠한 영향을 미치는지 알아보기 위해, 국산천일염, 중국산천일염, 정제염의 세 가지 소금으로 27%의 염농도로 새우젓을 제조한 후 2$0^{\circ}C$ 항온기에서 180일간 숙성 및 발효가 진행되는 동안 이화학적 변화(pH, 염도, 휘발성 염기질소, 아미노태질소, 색상)를 분석하였다. 세 가지 소금의 새우젓 모두 숙성이 진행되면서 단백질 분해 산물인 휘발성 염기질소, 아미노태 질소의 함량이 증가하였는데, 국산 천일염 새우젓이 더 많이 증가하였다. 즉, 숙성 180일 후 휘발성염기질소, 아미노태 질소 함량은 각각 국산천일염 새우젓의 경우 200mg%, 749mg%, 2.3lmg%인 반면, 중국산천일염 새우젓은 67.23mg%, 630mg%, 1.22mg%이었고, 정제염 새우젓은 100.85mg%, 634mg%, 1.76mg%로 나타났다. 지방산패도는 국산천일염 새우젓은 23일(2.450), 중국산천일염 새우젓은 58일(1.771), 정제염 새우젓(2.514)까지 증가한 후 감소하였다. 이상의 결과로 소금의 종류가 새우젓의 숙성에 영향을 미치는 것을 알 수 있었다.

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Plant Regeneration from Cryopreserved Embryogenic Cell Suspension Cultures of Korean Rice (Oryza sativa L.) Cultivars (한국 벼 품종 배발생 현탁배양 세포의 초저온 보존과 식물체 재분화)

  • 김석원;정원중;민성란;배경숙;유장렬
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.2
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    • pp.115-120
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    • 1995
  • A method for cryopreservation of suspension cultured embryogenic cells derived from immature zygotic embryos of rice (Korean cultivars, Donggin-byeo and Taebaeg-byeo) was developed. The highest cell regrowth after storage in liquid nitrogen was obtained when Donggin-byeo cells were cryoprotected with a mixture of 2 M DMSO and 0.4 M sucrose and Taebaeg-byeo cells with a mixture of 0.64 M DMSO and 0.4 M sucrose at frequencies of 88% and 90%, respectively, Pretreatment in a high osmotic medium was not necessary. Upon transfer to $N_{6}$ medium suplemented with lmg/L NAA and 5 mg/L kinetin, the regenerated calli gave rise to numerous somatic embryos which subsequently underwent development into plantlets. Among approximately 100 plantlets, 25% of them were albinos.s.

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Detection of flavonoid compounds by cell culture of Ginkgo biloba L (은행(Ginkgo biloba L.)의 세포배양에 의한 Flavonoid류의 검출)

  • 김광수;백윤웅
    • KSBB Journal
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    • v.11 no.1
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    • pp.1-7
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    • 1996
  • Calli induced from Ginkgo bilha L. were cultured to investigate optimal culture conditions and identify the possibility production of useful compounds. Calli were obtained from leaves and stems of Ginkgo biloba seedlings and embryos on WP medium supplemented with 2mg/$\ell$ NAA and 5mg/$\ell$ kinetin. Chlorophyll-ricked green callus was inducted in MS liquid medium containing 1mg/$\ell$ NAA and 0.1mg/$\ell$ kinetin under light as 3 clones selected with origin. Embryo derived callus showed the highest growth rate. Analysis for flavonoids and their precursor was performed by TLC and EMS. A specific precursor of flavonoid was identified in callus, not in natural leaves. These findings indicate that tissue culture may produce rlavonoids.

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A report of 7 unrecorded bacterial species isolated from several Jeju soil samples in 2016

  • Kim, Ju-Young;Jang, Jun Hwee;Maeng, Soohyun;Kang, Myung-Suk;Kim, Myung Kyum
    • Journal of Species Research
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    • v.7 no.2
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    • pp.151-160
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    • 2018
  • Seven bacterial strains, 15J4M-1, 15J13-8, 16MFM10, 15J1-8, SR1-5-4, 15J13-6, and 15J8-11 assigned to the phylum Actinobacteria, Bacteroidetes, and Firmicutes were isolated from soil samples collected from Jeju, Korea. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strains 15J4M-1, 15J13-8, 16MFM10, 15J1-8, SR1-5-4, 15J13-6, and 15J8-11 were most closely related to Bacillus selenatarsenatis $SF-1^T$ (with 99.4% similarity), Brevibacterium luteolum $CF87^T$ (99.5%), Carnobacterium iners CCUG $62000^T$ (99.6%), Exiguobacterium profundum $10C^T$ (99.3%), Larkinella insperata LMG $22510^T$ (99.3%), Pseudokineococcus lusitanus CECT $7306^T$ (99.4%), and Spirosoma endophyticum $EX36^T$ (99.3%), respectively. This is the first report of these seven species in Korea.

Efficient Plant Regeneration from Shoot Tip and Young Leaf in Rhodiola sachalinensis A. Bor.

  • Chi, Hyung-Joon;Yoon, Jae-Ho;Yang, Deok-Chun;Song, Won-Seob
    • Plant Resources
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    • v.6 no.3
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    • pp.233-241
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    • 2003
  • The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.

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A Novel Kinesin-like Protein, Surhe is Associated with Dorsalization in the Zebrafish Embryos

  • Kim, Eun-Joong;Ro, Hyun-Ju;Huh, Tae-Lin;Lee, Chang-Joong;Choi, Jin-Hee;Rhee, Myung-Chull
    • Animal cells and systems
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    • v.12 no.4
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    • pp.219-230
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    • 2008
  • We are reporting the expression patterns and possible biological functions of a novel Kinesin-like protein, Surhe, in the zebrafish. Homology studies of derived amino acid sequences suggest that Surhe has an amino-terminal kinesin motor domain that is similar to that of the emerging MKLP-1 subfamily [Kim and Endow, 2000] and two coiledcoil domains in a central region. Cellular localization studies in mammalian cells revealed that Surhe protein is located in cytoplasm, suggesting that Surhe may be involved in the intracellular transport. During the developmental process, surhe transcripts are highly expressed in early embryonic stages. Overexpression of the dominant negative form of Surhe significantly down-regulates the dorsalization markers, such as goosecoid, bozozok, and chordin. Taken together, we postulate that Surhe may be involved in dorsalization process as a motor molecule.

High capacity polymer for nickel determination in environmental samples

  • Panahi, Homayon Ahmad;Feizbakhsh, Alireza;Dadjoo, Fatemeh;Moniri, Elham
    • Advances in environmental research
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    • v.2 no.4
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    • pp.309-321
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    • 2013
  • High AA new high capacity sorbent for preconcentration and determination of nickel in environmental samples was synthesized. The sorbent was synthesized by copolymerization of allyl glaycidyl ether / imminodiacetic acid with N,N-dimethylacrylamide as functional monomers in the presence of N,N-bismethylenacryl amid as cross linker and characterized by Fourier transform infra red spectroscopy, elemental analysis, thermogravimetric analysis and scanning electron microscopy. A recovery of 93.6% was obtained for the metal ion with 0.1 M, sulfuric acid as the eluting agent. The sorption capacity of the functionalized sorbent was 55.9 $mgg^{-1}$. The equilibrium sorption data of Ni(II) on polymeric sorbent were analyzed using Langmuir, Freundlich, Temkin and Redlich.Peterson models. Based on equilibrium adsorption data the Langmuir, Freundlich and Temkin constants were determined 0.87 (L mg-1), 25.87 ($mgg^{-1}$) $(Lmg^{-1})^{1/n}$ and 171.4 ($Jmol^{-1}$) respectively at pH 4.5 and $20^{\circ}C$.

Calibrating black hole mass estimators using high quality Keck spectra

  • Park, Da-Woo;Woo, Jong-Hak
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.1
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    • pp.52.1-52.1
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    • 2011
  • Black hole masses of Active Galactic Nuclei (AGN) are one of the most important parameters in AGN physics. Based on the virial assumption, black hole masses can be determined from the product of the width of the broad emission lines and the continuum/line luminosities. Using the Low Resolution Imaging Spectrometer(LRIS) at the Keck telescope, we obtained high quality spectra (S/N~100), covering 2300-5500A in the rest-frame, for a sample of 37 intermediate-luminosity AGN at z~0.4, in order to calibrate various black hole mass estimators based on the Mg II (2798A) and the Hbeta (4861A) emission lines. After subtracting continuum and complex FeII emission under Mg II and Hbeta, we fit the broad emission lines using high order Guass-Hermite models to best constrain the profile and the width of the emission lines. Combining the SDSS spectra covering Halpha emission line with the Keck spectra, we determine a set of 6 black hole masses for each object, based on the line width (MgII, Hbeta, and Halpha) and the luminosity (LMgII, LHbeta, LHalpha, L3000, L5100), and calibrate each black hole mass estimator. We will present uncertainties and limitations of each mass estimator.

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Studies on the Multiplication and Induction of Hybrid Plant in Cremastra appendiculata Use the the Embryo and Tissue Culture (배배양 및 조직배양을 이용한 약난초 (Cremastra appendiculata) 의 증식과 잡종식물의 유도에 관한 연구)

  • 이정석;황백김영준
    • KSBB Journal
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    • v.5 no.1
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    • pp.43-47
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    • 1990
  • It was determined optimal Culture conditions and suitable growth regulators for seed germination, growth of callus, and protoplasts derived from cultured and mesophyll cells in Gremastra appendiculata. Induction of fusion between protoplasts of cultured and mesophyll cells was examined. The best conditions of seed germination and growth of callus were achieved on Hyponex medium contained plant growth regulators(2mg/l 2, 4-D, lmg/l Kinetin). Viability and regeneration of cell wall in protoplasts was determined with fluorescence microscope. Also, fused protoplasts were achieved by using PEG solution between protoplasts of cultured and mesophyll cells.

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Preparation of Ferroelectric $\textrm{SrBi}_{2}\textrm{Ta}_{2}\textrm{O}_{9}$ Thin Films Deposited by Plasma-enhanced Metalorganic Chemical Vapor Deposition (플라즈마를 이용한 유기금속 화학증착법에 의한 강 유전체 $\textrm{SrBi}_{2}\textrm{Ta}_{2}\textrm{O}_{9}$ 박막의 제조)

  • Seong, Nak-Jin;Kim, Nam-Gyeong;Yun, Sun-Gil
    • Korean Journal of Materials Research
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    • v.7 no.2
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    • pp.107-113
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    • 1997
  • $SrBi_{2}Ta_{2}O_{9}(SBT)$ thin films wcre prepared on $Pt/Ti/SiO_{2}/Si$ suhsrrate by pL~snia-enhanced chemical vapor deposition. Sr and Ta huhhling temperatures were kept ,it $120^{\circ}C$ Iron1 X- ray tiiffriict!on. n~icrostruc~ure. and composjrional analysis of SH7' films, respectivels Hi I~ut~t~lmg tempcl.arure was varied SR'I' thin tilrns dcpositcd ar i3i buhbling temperature of $130^{\circ}C$ have dielccrric constanr of 150 anti dissipation factor of 0 02 at IOOkFic. I .eakagc wrrent density of films was ahour $1.0{\times}10^{-8}A/cm^2$ at 20kV/cm. 1.eakage current i11amcrc1istic.s of Sli'l' films nras c.ontrolled by I'oole Frcnkel emission Kenianent polariziit~on and mercivc field oi SR\ulcorner' films annealed at $550^{\circ}C$ were $9{\mu}C/cm^2$ and 70kV/cm, respectively.

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