• Asian-Australasian Journal of Animal Sciences
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• v.5 no.2
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• pp.337-339
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• 1992

Successful induction of lactation in 5 nulliparous repeat breeding cows was achieved by administering diethyl stillboestrol (0.1 mg/kg B.W.) and hydroxyprogesterone caproate (0.25 mg/kg B.W.) for 3 days followed by reserpine (4 mg/day) for 4 days, and dexamethasone (16 mg/day) for one day. The treatment caused cystic ovarian condition in all the animals. Intravaginally progesteronesoaked sponges suppressed but did not eliminate the behavioural estrus. Administration of LHRH ($300{\mu}g$) regressed the cysts in all animals in the first instance but the cysts reappeared in 2 animals. One animal became pregnant and delivered a normal calf.

• Clinical and Experimental Reproductive Medicine
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• v.21 no.1
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• pp.43-48
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• 1994

To evaluate the effectiveness of GnRH agonist for the treatment of uterine myoma as a cause of infertility, fourteen women were recruited to the study. The patients were treated with a delayed-release formulation of D-$Trp^6$-LHRH in biodegradable microcapsules(Decapeptyl-CR), administered intramuscularly at four week intervals for a period of six monthes. The first injection was given on day 21 of the cycle. Serum estradiol levels fell significantly to the mean value of 257.7pgjml 4 weeks after the first injection. Eleven patients in fourteen treated patients had a reduction in the size of uterine myoma as assessed by ultrasonography, two patients had no change of size and one patient had a increase of size. After the first or second injection, all patients became amenorrheic, then resumption of menstruation ocurred at 12 to 14 weeks after the last injection. Common side effects were hot flush, sweating and dyspareunia, whitch were acceptale. In Eleven patients who had a reduction in the size of uterine myoma by treatment with a delayed- release formulation of D-$Trp^6$-LHRH(Decapeptyl-CR), after above treatment with GnRH agonist, then four patients were treated with myomectomy, three patients had pregnancy and full term delivered by Cesarean section. These data suggest that administration of a delayed-release formulation of a GnRH agonist can be a worthwhile and convenient approach to the medical treatment of uterine myoma as a cause of infertility.

• Proceedings of the PSK Conference
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• 2001.04a
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• pp.235.1-235.1
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• 2001

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.62-62
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• 2003

최근 연안 자원이 급격하게 감소하고 있고 새로운 양식 대상종으로 평가되고 있는 말쥐치의 대량 종묘생산을 위해서는 수정난 대량확보와 자어기 먹이생물 구명 등의 문제점이 해결되어야 한다. 본 연구자들은 우선 호르몬 처리에 의한 산란유도, 조기성숙유도에 의한 조기산란유도 및 수정난 확보방법을 개발하고, 위내용물 분석을 통한 자어의 초기먹이생물을 구명하고자 하였다. 산란유도를 위하여 hCG, LHRH-a 및 hCG+LHRH-a를 다양한 농도로 처리하여 실험한 결과 모든 실험구에서 67~76%의 수정률을 나타내었으나, 대량의 성숙란을 얻지는 못하였다. 따라서 본 종의 산란특성이 다회성인 점을 감안한다면 500 g 전후의 친어 200~300마리를 1개의 수조에 수용하여 자연산란된 수정란을 수거하는 것이 좋을 것으로 판단되었다. 평균 450 g 정도의 말쥐치 친어를 12월부터 6개월동안 천천히 수온을 상승시켜 자연산란을 유도한 결과, 자연산란기 (6월)보다 2개월 빠른 4월 15일(17$^{\circ}C$로 가온한 지 15일)에 첫 산란이 이루어졌고 1개월간 지속되었다. 초기 사육환경을 구명하기 위한 실험에서는 일반적으로 해산어 종묘 생산에 이용하는 클로렐라＋rotifer 실험군이 클로렐라 단독, 규조류 단독, 규조＋rotifer, 녹조＋copepods등이 번식된 환경보다 생존율에 있어 월등히 높은 결과를 나타내었다. 위 내용물 분석에 의한 사육기간 중 섭식한 주요 먹이생물종은 부화 후 5일부터 20일째까지는 rotifer egg, 부화 10일째는 rotifer egg와 small rotifer, 부화 20일째는 rotifer, Artemia sp., copepods, 부화 25일째부터는 copepods였다. 초기성장은 부화직후 전장 1.95 mm였던 것이 10일째는 2.96 mm, 20일째는 3.89 mm, 30일째는 12.9 mm, 50일째는 35.1 mm를 나타내었다. 그러나 말쥐치 자ㆍ치어가 성장함에 따라 발생하는 지속적인 폐사와 종묘의 수송시 발생하는 폐사를 줄일 수 있는 방법이 추후에 해결되어야 할 것으로 사료된다.

• Clinical and Experimental Pediatrics
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• v.46 no.4
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• pp.400-403
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• 2003

Congenital adrenal hyperplasia(CAH) is a recognized cause of precocious pseudopuberty. Some children with CAH also develop true precocious puberty with early maturation of the hypothalamic-pituitary-gonadal axis. We review a case of CAH who eventually developed central precocious puberty nine months after initial treatment with corticosteroid. A 3-year-old boy visited complaining of rapid growth, a large penis and frequent penile erections. This patient was diagnosed with CAH with elevated 17-OH progesterone and cortical hypertrophy of adrenal gland on CT scan. His gonadotropin levels were within the normal prepubertal range. Even on treatment with corticosteroid he grew rapidly and had testicular enlargement, pubic hair development and rapid bone maturation. At second admission, his gonadotropin levels were elevated both basally and in response to LHRH stimulation, suggesting that the CAH led to early activation of pubertal gonadotropin secretion(true precocious puberty). He was treated with monthly depot injections of a LHRH analog in addition to the hydrocortisone. His second sexual characteristics regressed gradually and rate of linear growth and bone maturation decreased.

• Korean Journal of Ichthyology
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• v.24 no.2
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• pp.94-100
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• 2012

The spine loach, Cobitis hankugensis endemic to Korea were collected at the Nokdong River, Unbong-eup, Namwon-si, Jeollabuk-do during June 2007 for study its eggs development and early life history. The eggs were obtained by injecting gonadotropin (LHRH-1) to female and were fertilized by dry method in the laboratory condition. The matured eggs were separative, demersal and light yellowish with $1.29{\pm}0.07mm$ in diameter average. Under $23{\sim}25^{\circ}C$, the eggs were hatched in 45~52 hours after fertilization, and just hatching larvae were $4.5{\pm}0.24mm$ in total length. On 5 days after hatching, they were $6.6{\pm}0.13mm$ in total length and their yolk sacs were completely absorbed. From 25 days after hatching, they became the juvenile stage with $9.0{\pm}0.49mm$ in total length. On the 100 days after hatching, their external forms and band patterns were similar to those of adults with average $27.3{\pm}2.82mm$ in total length.

• Korean Journal of Ichthyology
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• v.21 no.1
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• pp.15-22
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• 2009

An artificial breeding method was carried out to investigate development of eggs and the early life history of Iksookimia longicorpa. The eggs of the female were obtained by injecting gonadotropin (LHRH) and fertilizingby the dry method in the laboratory. The mature eggs were separate, demersal, light yellowish, and averaged $1.53{\pm}0.07mm$ in diameter. In water temperature of $23{\sim}25^{\circ}C$, the eggs hatched in 50~72 hours after fertilization, and their lengths reached $5.1{\pm}0.23mm$ in total length. On Day 4 after hatching, they averaged $7.1{\pm}0.47mm$ in total length and their yolk sacs were completely absorbed. From 25 days after hatching, they attained a juvenile stage reaching $12.8{\pm}0.49mm$ in total length. On the 100th dayafter hatching, their band patterns and external form were similar to those of adults, and they averaged $38.5{\pm}2.95mm$ in total length.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.300-301
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• 2000

고급단백질의 수요가 증가함에 따라 양식어류개발의 필요성이 가중되면서 유용어종을 대상으로 계획적이고 안정적인 대량종묘를 확보하기 위해서는 인위적으로 성숙/산란을 조절할 수 있는 방법을 개발하는 것이 우선 과제이다. 이러한 방법에는 적합한 호르몬제를 사용하던지 또는 사육 환경조건을 변화시키는 방법 등이 있는데(Donaldson and Hunter, 1983) 호르몬제를 사용할 경우, 자연산란과 비교해볼 때 난질이 좋지않아 수정률 저하를 초래할 경우가 많다. 지금까지 양식현장에서는 HCG(태반선성선자극호르몬)가 널리 사용되어왔으나, 최근에는 GnRH (gonadotropin hormone-releasing hormone)를 이용한 성숙/배란유도가 성행되고 있으며 이에 대한 투여방법도 및 가지로 연구되고 있다 (Okumura and Sakae, 1993; Leu and Chou, 1996). (중략)

• Proceedings of the Korean Biophysical Society Conference
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• 1996.07a
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• pp.28-28
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• 1996

NMR studies on the structure of the luteinizing hormone releasing hormone (LHRH) in aqueous buffer and trifluoroethanol (TFE)/aqueous buffer (1:1, v/v) solution were performed. The NMR data under these conditions suggested a unique conformation which includes a ${\beta}$-1 turn of the Tyr5-Arg8 segment and an unusual turn of Ser4-Gly6 segment staggered with the ${\beta}$-I turn. (omitted)

• Journal of Embryo Transfer
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• v.24 no.4
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• pp.271-274
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• 2009

To establish a protocol of estrus induction and synchronization in European mouflon, we performed artificial insemination using frozen-thawed semen and exogenous hormones. CIDR was inserted into vaginas of four mouflons for 16 days. A day before removal of CIDR, PG 600 was injected intramuscularly. $PGF_2{\alpha}$ was injected when removing CIDR. Artificial insemination was cervically conducted with injecting LHRH 48 hours after CIDR withdrawal. Even though no pregnancy was confirmed, estrous signs were notified like open cervix, congestion of vaginal wall and discharge of cervical mucus. Further research in the wild sheep would be needed for development of artificial breeding methods and advancing sustainability of domestic zoos.