• The Journal of Internal Korean Medicine
• /
• v.29 no.2
• /
• pp.359-374
• /
• 2008

Objective : Hyperhidrosis is a condition characterized by excessive sweating. Some studies suggest that hyperhidrosis is associated with autonomic nervous system dysfunction. Hyperhidrosis is often accompanied by hypersensitivity, tension, irritability, heat flashes, fatigue, etc. This study was designed to evaluate the correlation between Oriental Medicine diagnosis and the autonomic nervous system function in patients with hyperhidrosis using Heart Rate Variability (HRV) analysis. Methods : 23 palmar and plantar hyperhidrosis patients and 10 systemic hyperhidrosis patients were recruited and evaluated by Oriental Medicine diagnostic questionnaire and HRV analysis. The Oriental Medicine diagnostic questionnaire used a three-dimensional diagnosis that classified patients into Cold or Heat Syndrome, Yin or Yang Syndrome, and Deficiency or Excess Syndrome. Measured indices of HRV used the frequency domain analysis(i.e. TP, VLF, LF, HF and LF/HF ratio). Also, measure indices of HRV adjusted for aging effects were evaluated. Results : Cold/Heat Syndrome was not associated with hyperhidrosis prevalence nor the HRV analysis in hyperhidrosis patients. The Yang Syndrome group(78.8%) was more strongly correlated than the Yin Syndrome group(21.2%), and character of Yang was correlated with the adjusted TP and adjusted LF. The LF/HF ratio in the Excess Syndrome group was significantly higher than the LF/HF ratio in the Deficiency Syndrome group. Conclusion : Hyperhidrosis was not associated with the Cold/Heat Syndrome, but was found to be closely associated with the Yang Syndrome. The LF/HF ratio was significantly higher in the Excess Syndrome group than in the Deficiency Syndrome group in hyperhidrosis patients.

• Journal of Korean Medicine Rehabilitation
• /
• v.23 no.1
• /
• pp.51-64
• /
• 2013

Objectives : This study was performed to evaluate the effect of dry cupping treatment applied to back-shu points on the autonomic nervous system. Methods : Two groups of sympathicotonia and normal with each 30 volunteers were set up for this experiment. The sympathicotonia group was selected by the criterion for sympathicotonia by the questionnaire composed of 11 items. After 10 minutes for environmental adaptation, the first HRV(heart rate variability) test was conducted, and then, dry cupping therapy was applied to back-shu points for 5 minutes to stimulate sympathetic ganglia lying along the spine. The second HRV test was carried out just after the cupping therapy under the same condition and then, the third test was repeated after two hours based on the first test time. Results : 1. In sympathicotonia group, SDNN(standard deviation of all normal R-R intervals), RMSSD(the square root of the mean of the sum of the squares of differences between adjacent normal R-R intervals), Ln(HF)(high frequency power), nmHF(normalized high frequency power) increased and mHR(mean heart rate), nmLF(normalized low frequency power) decreased significantly right after dry cupping therapy which means dry cupping affects on autonomic nervous system. The effect lasts in these items of nmLF, Ln(HF), nmHF, rLHF(rate ratio of LF/HF). 2. In normal group, SDNN, RMSSD increased and mHR decreased significantly right after dry cupping therapy, too. But, Ln(LF)(low frequency power), nmLF, rLHF unexpectedly increased and nmLF, rLHF stay increased up to 2 hours. Conclusions : The results suggest that the dry cupping therapy has effect on the autonomic nervous system. It is effective to stabilize hyper-sympathetic tone of people diagnosed as Sympathicotonia and activate parasympathetic tone.

• Journal of Acupuncture Research
• /
• v.25 no.2
• /
• pp.189-197
• /
• 2008

Objectives : This study was performed in order to investigate the effectiveness of electromyography and the Heart Rate Variability(HRV) test as prognosis factors, and to clarify correlation between Electromyography and the Heart Rate Variability test. Methods : 44 Bell's palsy patients who were graded V on the House-Brackmann scale and underwent HRV and EMG testing were retrospectively reviewed based on medical records. Results from both tests were analyzed via simple linear regression, and bivariate correlation analysis was performed to investigate the correlation between results from the two tests. The severity of the facial palsy at onset and at 2 weeks after treatment were evaluated with the H-B grade and Yanagihara grading system, and was converted into improvement scores. Results : Mean axonal loss according to electromyography showed a statistically significant correlation in predicting peripheral facial palsy improvement(p<0.01). HR, SDNN, TP, LF, HF, VLF, and LF/HF ratio on the Heart Rate Variability test showed no significant correlation in predicting peripheral facial palsy improvement. Mean axonal loss determined by electromyography, and HR, SDNN, TP, LF, HF, VLF, and LF/HF ratio recorded with the Heart Rate Variability test was analyzed with the bivariate correlation analysis method. Mean axonal loss and SDNN showed a statistically significant correlation(p<0.01) Conclusions : The Heart Rate Variability test has no statistical significance in predicting peripheral facial palsy improvement. SDNN has a statistically significant correlation with mean axonal loss as determined by electromyography.

• Bulletin of the Korean Chemical Society
• /
• v.25 no.2
• /
• pp.237-242
• /
• 2004

Recombinant human ferritin homopolymers (rHF and rLF) were successfully produced in the Saccharomyces cerevisiae Y2805, which was transformed with human ferritin H or L-chain genes, respectively. In order to characterize the molecular properties of the recombinant ferritins in relation to mineralization, the proteins were isolated and apoferritins were prepared. The apoferritins were reconstituted with 2000 Fe atoms per protein molecule under various experimental conditions (the concentration of the protein, the buffer concentration of the MOPS buffer, the total volume of the reaction and the reconstitution method). The structure and composition of the iron cores formed in the ferritins were examined using transmission electron microscopy. The recombinant ferritins behaved in a similar manner to other mammalian ferritins in accumulating iron in the core. Proteins of rHF and rLF showed varying reconstitution yields of 37-72% depending on the reaction conditions. In general, the rHF showed higher reconstitution yield than the rLF at the protein concentrations and the reaction volumes we examined. Iron cores with a similar mean particle size were obtained in the rHF, rLF and horse spleen ferritin reconstituted at a protein concentration of 1.0 mg/mL. Electron diffraction of all the three ferritins showed 2-3 diffuse lines, with d-spacings corresponding to those of the mineral ferrihydrite with a limited crystallinity.

• Journal of Oriental Neuropsychiatry
• /
• v.19 no.3
• /
• pp.45-54
• /
• 2008

Objective: To treat psychogenic headache patients, doctors have to amplify on the headache caused by emotional stress to patients, and assist the patients to cope with difficulties. So, we investigated HRV of the headache patients caused by Chiljungsang and would like to apply to the clinical treatment. Method: Our study measured time and frequency domain HRV indicies(5-min resting study) of 123 headache patients caused by emotional stress. Standardized tests of HRV allow a quantitative estimation of autonomic nervous system function. Results & Conclusions: 1. The study classed as aspects of the head pain showed the differences in RMS-SD(square root of mean squared difference of successive NN intervals) band, HF(high frequency) band significantly. 2. The male headache patients showed higher all the indicies except heart rate compared to the female patients, significantly in SDNN(standard deviation of NN interval), TP(total Power), HF band. 3. As the patients grow older, SDNN, RMS-SD band was lower and LF(low frequency) band, LF/HF ratio higher significantly. The beginning age lower, SDNN, RMS-SD band was higher significantly. The duration of the disease longer, LF band, LF/HF ratio was higher significantly.

• Journal of Acupuncture Research
• /
• v.24 no.4
• /
• pp.13-23
• /
• 2007

Objectives : The aim of the present study was to assess the effects of Sa-am acupuncture (Damjeonggyeok) on autonomic nervous system in night nurses by heart rate variability. Methods : Five night nurses were divided into two groups. We checked their HRV once after sleeping for night and 5 times after working for night, 4 times after operation. We operate Sa-am acupuncture (Damjeonggyeok) and sham acupuncture twice by turns for group A and in reverse order for group B. We checked responses of HRV change after operations. Statistical analysis was performed by using the SPSS Win. Ver. 13.0 for analysis about correlation between data, and Baysian analysis using WinBUGS(Ver. 1.4) for comparison between Sa-am acupuncture (Damjeonggyeok) and sham acupuncture. Results : The median overall decrease for difference in responses of low-frequency power in normalized units (LF (NU)) was -16.56 (-28.88, -6.446), the median overall increase for difference in responses to high-frequency power in normalized units (HF (NU)) was 16.25 (8.104, 28.6) and for the median overall decrease for difference in responses to the ratio of low- to high-frequency power (LF/HF) was -1.846 (-3.922, -0.069), 95% credibility intervals being shown in brackets. VAS of stress was correlated with LF (NU) and HF (NU), LF/HF (p<0.0l). Conclusion : Sa-am acupuncture (Damjeonggyeok) might have sympatholytic and parasympathomimetic effects.

• Journal of Oriental Neuropsychiatry
• /
• v.21 no.2
• /
• pp.75-85
• /
• 2010

Objectives : This study was designed to evaluate the correlation between patterns of dyspepsia(acute dyspepsia, functional dyspepsia, dyspepsia with the organic disease) and autonomic nerve system dysfunction using HRV analysis. Methods : The patient group consisted of 88 patients diagnosed as a dyspepsia (Acute dyspepsia group-35 patients, Functional dyspepsia group-28 patients, Dyspepsia with the organic disease group-28 patients) who visited in the Oriental Medical Hospital of Dong-eui University from 2005.3.OO to 2008.8.OO. And the control group consisted of 33 patients diagnosed as a normal state of stomach during the same period. We checked HRV of the 4 groups over 5 minutes and compared the HRV index between groups. Results : 1. HF, LF, VLF and TP were significantly lower in the acute dyspepsia, functional dyspepsia and organic dyspepsia patient group than in the control group. 2. HF, LF, VLF and TP were higher in the acute dyspepsia patient group than in the organic dyspepsia patient group, but the differences were not statistically significant. 3. HF, LF, VLF and TP were higher in the acute dyspepsia patient group than in the functional dyspepsia patient group, but the differences were not statistically significant. 4. HF, LF, VLF and TP were lower in the functional dyspepsia patient group than in the organic dyspepsia patient group, but the differences were not statistically significant. Conclusions : Compared to the control group, all of the dyspepsia patient groups showed the tendency that the overall activity of the autonomic nervous system and the activity of sympathetic nerves decreased. Although there was no significant difference in the suppression of the autonomic nervous system, chronic dyspepsia patient group was lower than acute dyspepsia patient group, functional dyspepsia patient group was lower than the organic dyspepsia patient group in HRV.

• Journal of the Ergonomics Society of Korea
• /
• v.31 no.6
• /
• pp.785-791
• /
• 2012

Objective: The aim of this study was to investigate the correlation between 3D visual fatigue and physiological measures by canonical correlation analysis enabling to categorical correlation. Background: Few studies have been conducted to investigate the physiological mechanism underlying the visual fatigue caused by processing 3D information which may make the cognitive mechanism overloaded. However, even the previous studies lack validation in terms of the correlation between physiological variables and the visual fatigue. Method: 9 Female and 6 male subjects with a mean age of $22.53{\pm}2.55$ voluntarily participated in this experiment. All participants were asked to report how they felt about their health sate at after viewing 3D. In addition, Low & Hybrid measurement test(Event Related Potential, Steady-state Visual Evoked Potential) and for evaluating cognitive fatigue before and after viewing 3D were performed. The physiological signal were measured with subjective fatigue evaluation before and after in watching the 3D content. For this study suggesting categorical correlation, all measures were categorized into three sets such as included Visual Fatigue set(response time, subjective evaluation), Autonomic Nervous System set(PPG frequency, PPG amplitude, HF/LF ratio), Central Nervous System set(ERP amplitude P4, O1, O2, ERP latency P4, O1, O2, SSVEP S/N ratio P4, O1, O2). Then the correlation of three variables sets, canonical correlation analysis was conducted. Results: The results showed a significant correlation between visual fatigue and physiological measures. However, different variables of visual fatigue were highly correlated to respective HF/LF ratio and to ERP latency(O2). Conclusion: Response time was highly correlated to ERP latency(O2) while the subjective evaluation was to HF/LF ratio. Application: This study may provide the most significant variables for the quantitative evaluation of visual fatigue using HF/LF ratio and ERP latency based human performance and subjective fatigue.

• Journal of Korean Society of Forest Science
• /
• v.97 no.6
• /
• pp.634-640
• /
• 2008

The purpose of this study is to clarify the physiological effects of Shinrin-yoku (taking in the atmosphere of the forest of forest bathing). The subjects were twelve male university students ($21.6{\pm}2.1$ years old). On the first day, six subjects were sent to a forest area, and the others to a city area. On the second day, the subjects were sent to the opposite areas for a cross check. The subjects were seated on chairs and viewing the landscapes of their given areas for 15 minutes. HRV (Heart Rate Variability) and diastolic blood pressure were used as physiological indices. Diastolic blood pressure and LF/(LF+HF) components of HRV in the forest area were significantly lower than in the city area. And HF power components of HRV in the forest settings were significantly enhanced than in city settings. In conclusion, the results of the physiological measurements show that Shinrin-yoku was able to effectively relax people in both mind and body.

• Korean Journal of Oriental Medicine
• /
• v.13 no.3
• /
• pp.97-103
• /
• 2007

The object of this study is to evaluate confounding effect of sleeping behavior on heart rate variability (HRV) in healthy volunteers during a randomized clinical trial. From 6 healthy volunteer, we acquired circadian electrocardiography(ECG) with a Holter monitoring device(DigiTrak Plus, Philips). The compatible analytical program, Zymed, was used for calculation of RR intervals of 24h ECG signal. Then, we generated HRV signals and calculated 9 HRV parameters in time domain and frequency domain for every 30 minute with Matlab 6.x software. We also encoded participants' activities as follows; stable condition(1), sleeping behavior(2), eating behavior(3), and undefined condition(4). Using SPSS 12.0, we tested if there was any difference between HRV parameters of day-time and night-time and between those of a subject in stable condition and in sleeping behavior. As a result, one of the most important parameter-LF/HF ratio, which indicates the autonomic balance between sympathetic and parasympathetic nervous systems, as same the other parameters, showed large inter-individual variations in circadian profiles. And, the mean LF/HF ratio of each participant was different between in stable condition and in sleeping behavior (p<.05). Consequently, the effect of sleeping behavior must be considered when one or more of HRV parameters, especially LF/HF ratio, are used as assessment index in clinical studies.