• Molecules and Cells
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• 제38권5호
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• pp.457-465
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• 2015

The 15-kDa selenoprotein (Sep15) is a selenoprotein residing in the lumen of the endoplasmic reticulum (ER) and implicated in quality control of protein folding. Herein, we established an inducible RNAi cell line that targets Sep15 mRNA in Chang liver cells. RNAi-induced Sep15 deficiency led to inhibition of cell proliferation, whereas cell growth was resumed after removal of the knockdown inducer. Sep15-deficient cells were arrested at the G1 phase by upregulating p21 and p27, and these cells were also characterized by ER stress. In addition, Sep15 deficiency led to the relocation of focal adhesions to the periphery of the cell basement and to the decrease of the migratory and invasive ability. All these changes were reversible depending on Sep15 status. Rescuing the knockdown state by expressing a silent mutant Sep15 mRNA that is resistant to siRNA also reversed the phenotypic changes. Our results suggest that SEP15 plays important roles in the regulation of the G1 phase during the cell cycle as well as in cell motility in Chang liver cells, and that this selenoprotein offers a novel functional link between the cell cycle and cell motility.

• 한국수산과학회지
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• 제56권6호
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• pp.878-888
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• 2023

Growth responses of Chlorella vulgaris exposed to different light intensities and wavelengths of light-emitting diodes (LEDs) were investigated. C. vulgaris was cultured under red LED (650 nm), blue LED (450 nm), green LED (520 nm), and fluorescent lamps (three wavelengths, control). The maximum growth rates (µ_max) of C. vulgaris were highest under the blue LED, followed by the red LED, green LED, and fluorescent lamps. The low compensation photon flux density (I₀) and low half-saturation constants (K_s) were observed in C. vulgaris cultured under the red LED, indicating that high C. vulgaris growth is closely related to the low light intensity of the red LED suggesting that the red LED can be useful for the biomass production of C. vulgaris. Furthermore, it was observed that under the blue LED during the stationary phase, there was an increase in useful bioactive substances, such as proteins and lipids, which are beneficial for biomass production. In conclusion, the red LED is an economical light source that can enhance cell density, and the blue LED is effective in promoting valuable intracellular substances.

• 한국전기전자재료학회논문지
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• 제28권2호
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• pp.131-136
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• 2015

We develop the 60 W class hybrid solar LED street lamp controller. The controller is providing power via an inverter in the day with the least solar cell and battery and charging the battery for the ESS, acts as a power failure, the built-in battery. Rated output of the fabricated LED street lamp is 300 W or greater battery capacity 300 Wh, discharge time 10 hr, LED street lamp efficiency showed a very high level of light efficiency by about 127 lm/W. In addition, as a result of light distribution pattern according to the distance and the light intensity measurement will be able to ensure a very high quality, show the constant brightness in the distance from the road lights 6 m is about 35~40 lux in uniformity ratio. The proposed hybrid solar LED street lamp system showed a high energy capacity of approximately 1.5 to 152.7% power generation efficiency than typical conventional solar street lamp.

• Molecules and Cells
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• 제42권11호
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• pp.755-762
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• 2019

Despite decades of research into colorectal cancer (CRC), there is an ongoing need for treatments that are more effective and safer than those currently available. Lactic acid bacteria (LAB) show beneficial effects in the context of several diseases, including CRC, and are generally regarded as safe. Here, we isolated a Lactobacillus rhamnosus (LR)-derived therapeutic protein, p8, which suppressed CRC proliferation. We found that p8 translocated specifically to the cytosol of DLD-1 cells. Moreover, p8 down-regulated expression of Cyclin B1 and Cdk1, both of which are required for cell cycle progression. We confirmed that p8 exerted strong anti-proliferative activity in a mouse CRC xenograft model. Intraperitoneal injection of recombinant p8 (r-p8) led to a significant reduction (up to 59%) in tumor mass when compared with controls. In recent years, bacterial drug delivery systems (DDSs) have proven to be effective therapeutic agents for acute colitis. Therefore, we aimed to use such systems, particularly LAB, to generate the valuable therapeutic proteins to treat CRC. To this end, we developed a gene expression cassette capable of inducing secretion of large amounts of p8 protein from Pediococcus pentosaceus SL4 (PP). We then confirmed that this protein (PP-p8) exerted anti-proliferative activity in a mouse CRC xenograft model. Oral administration of PP-p8 DDS led to a marked reduction in tumor mass (up to 64%) compared with controls. The PP-p8 DDS using LAB described herein has advantages over other therapeutics; these advantages include improved safety (the protein is a probiotic), cost-free purification, and specific targeting of CRC cells.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2012년도 춘계학술대회
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• pp.743-744
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• 2012

다기능성 복합센서의 모듈로 구성된 신호처리시스템에서 사용자 정보 및 상황을 실시간 인지하여 조명환경 및 생활환경을 분석할 수 있는 LED 디지털제어 융합기술이 주목을 받고 있다. LED 조명은 고효율, 장수명, 친환경적 장점과 나아가 LED 조명과 통신의 융합이 가능하며, 백열등 및 형광등을 대체할 차세대 일반조명으로 각광받고 있다. 제안된 시스템은 태양광을 이용한 지능형 LED 제어 시스템으로서, 사용자 예측 정보/상황 연계형 조명제어 기술 및 이에 따른 절감기술에 관한 연구이다. 또한 방전 전류의 10%를 주위의 환경에 따라서 보조 유색 LED를 적절히 점등함으로서 감성조명을 구현하고자 한다.

• 해양환경안전학회지
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• 제29권1호
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• pp.36-43
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• 2023

본 연구는 Tetraselmis suecica와 T. tetrathele의 영양물질 증진효과를 가져올 수 있는 배양시스템 구축을 위하여, 발광다이오드(LED)의 파장별(청색; 450 nm, 황색; 590 nm, 적색; 630 nm) 탄수화물, 단백질, 지질 함량을 측정하였다. 두 종 모두 단백질 비율(42~69%)이 가장 높았으며, 생장속도가 낮았던 황색파장에서 탄수화물, 단백질, 지질의 높은 함량을 보였고, 생장속도가 가장 높았던 적색파장에서는 낮은 함량을 보였다. 이러한 결과는 세포 분열 속도의 감소로 인해 단백질 합성과 함께 세포의 화학적 조성과 효소 활동에 변화를 주어 지질과 탄수화물 함량이 증가한 것으로 생각된다. 따라서, T. suecica와 T. tetrathele의 유용한 생화학적 물질의 증대를 위해 대수생장기 초기와 중기는 적색 LED 그리고 대수생장기 후기에는 황색 LED를 주사하는 2단계 LED 배양을 제안하였다.

• 디지털융복합연구
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• 제14권5호
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• pp.325-331
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• 2016

야간에 가로등이 없는 지방 국도 또는 우천시에 과속 방지턱이 보이질 않아 과속방지턱에 거의 근접하여 급 브레이크를 밟는 경우가 종종 발생하여 사고가 발생한다. 또한, 논 또는 밭과 인접한 지방 국도의 경우 농작물의 피해를 줄이고자 가로등 설치를 못하는 실정이다. 이 경우 야간에 사람들이 길을 다니는 경우 특히 검은색 계열의 옷을 입고 다니는 경우 사람의 존재를 운전자가 인식하지 못해 잦은 사고가 발생한다. 이 경우, 파장이 긴 색깔의 LED 가로등을 설치하면 농작물의 피해를 주지 않고 사람의 존재만 구별할 수 있어 교통사고를 줄일 수 있다. 따라서, 본 논문에서는 이를 위하여 과속방지턱에 LED를 부착하여 점등함로써 사전의 속도를 줄여 사고를 줄일 수 있고 또한 농작물에 피해를 주지 않으면서 인사 교통사고를 줄일 수 있는 태양광 및 압전소자를 이용한 절전형 LED 자동점등 지방국도(논로) 교통사고 방지 및 과속방지턱 겸용 가로등을 개발하였다. 태양광 및 압전소자를 이용 전력을 축척하여 LED를 점등하는 방식으로 조도센서를 사용하지 않고 태양광 소자의 에너지 축적 여부를 이용하여 LED를 ON/OFF한다. 또한 압전소자도 이용함으로써 여름철 장마에 태양광에 의한 축적이 부족한 경우를 대비할 수 있고, 또한 저전력 설계를 구현하여 최소 3일 이상 무충전시에도 정상 작동하도록 구현하였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.97-97
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• 2010

We demonstrate the hybrid polymer-quantum dot based multi-functional device (Organic bistable devices, Light-emitting diode, and Photovoltaic cell) with a single active-layer structure consisting of CdSe/ZnS semiconductor quantum-dots (QDs) dispersed in a poly N-vinylcarbazole (PVK) and 1,3,5-tirs- (N-phenylbenzimidazol-2-yl) benzene (TPBi) fabricated on indium-tin-oxide (ITO)/glass substrate by using a simple spin coating technique. The multi-functionality of the device as Organic bistable device (OBD), Light Emitting Diode (LED), and Photovoltaic cell can be successfully achieved by adding an electron transport layer (ETL) TPBi to OBD for attaining the functions of LED and Photovoltaic cell in which the lowest unoccupied molecular orbital (LUMO) level of TPBi is positioned at the energy level between the conduction band of CdSe/ZnS and LiF/Al electrode (band-gap engineering). Through transmission electron microscopy (TEM) study, the active layer of the device has a p-i-n structure of a consolidated core-shell structure in which semiconductor QDs are uniformly and isotropically adsorbed on the surface of a p-type polymer core and the n-type small molecular organic materials surround the semiconductor QDs.

• 한국축산식품학회지
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• 제42권1호
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• pp.142-152
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• 2022

The present study was aimed at examining the antibacterial effects of nonthermal decontamination processes, which are equivalent to thermal treatment, to ensure microbiological safety of raw ground chicken. Escherichia coli or Salmonella were inoculated into 25 g of raw ground chicken samples. The raw ground chicken samples were non-treated or treated with high hydrostatic pressure (HHP) at 500 MPa (1-7 min), light-emitting diode (LED) irradiation at 405 nm wavelength (30-120 min), and heat at 70℃, 90℃ (1-60 min), and 121℃ (1-15 min). E. coli and Salmonella cell counts were enumerated after treatments. Moreover, the color parameters of treated raw ground chicken were analyzed. HHP treatment reduced E. coli and Salmonella cell counts by more than 5 Log CFU/g and more than 6 Log CFU/g after 7 min and 1 min, respectively; these effects were equivalent to those of thermal treatment. However, LED irradiation reduced Salmonella cell counts by only 0.9 Log CFU/g after 90 min of treatment, and it did not reduce E. coli cell counts for 90 min. Compared with those of the non-treated samples, the ΔE (total color difference) values of the samples treated with HHP were high, whereas the ΔE values of the samples treated with LED irradiation were low (1.93-2.98). These results indicate that despite color change by HHP treatment, HHP treatment at 500 MPa could be used as a non-thermal decontamination process equivalent to thermal treatment.

• 대한의생명과학회지
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• 제16권4호
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• pp.365-376
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• 2010

The purpose of this study was to clarify the effect of light emitting diode (LED) irradiation on healing of impaired wound and alteration of mast cells in experimental diabetic rats. Twenty-four male Sprague-Dawley rats were divided into four groups: excision (Ex), excision-LED irradiation (Ex-LED), diabetes + excision (DM) and diabetes + excision + LED irradiation (DM-LED). Diabetes was induced in rats by streptozotocin (STZ) injection (70 mg/kg, single dose) and 6 mm punch excision wounds were created on the back after shaving hair. The LED-irradiated rats were treated to a daily dose of $5\;J/cm^2$ LED (630 nm) light for 11 days after surgery, and were killed at day 1, 3, 7 and 11. The lesion and adjacent skin tissues were excised, fixed with 10% buffered formalin and embedded with paraffin. For evaluation of wound healing, hematoxylin-eosin (HE) and Masson trichrome staining were performed. Mast cells (MCs) were stained with toluidine blue (pH 0.5) and quantified using a computerized image analysis system. The proliferation activity of keratinocyte in skin tissues was analyzed on sections immunostained with proliferative cell nuclear antigen (PCNA). The results showed that wound healing rate, collagen density and neo-epidermis length, number of PCNA-positive cells, fibroblasts and mast cells were significantly higher in the LED-irradiated rats than in the DM and Ex rats throughout the periods of experiment. Exceptionally, the number of MCs was significantly lower at day 11 compared with day 7 after surgery in the all groups. These findings suggest that the LED irradiation may promote the tissue repair process by accelerating keratinocyte and fibroblast proliferation and collagen production in normal rats as well as in diabetic rats, and MCs may play an important role at an early stage of skin wound healing in normal and diabetic rats.