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High Frequency Bulblet Formation in Scale and Stem Thin Cell Layer Explant Cultures of Lilium Oriental Hybrids (오리엔탈 백합의 인편 및 줄기의 박판 세포층 절편으로부터 고빈도 자구형성)

  • Oh, Seung-Cheol;Chung, Myung-Hee;Kim, Suk-Won;Liu, Jang-Ryol
    • Journal of Plant Biotechnology
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    • v.30 no.3
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    • pp.251-255
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    • 2003
  • An efficient system for in vitro bulblet formation of Lilium oriental hybrids(cvs). Casa Blanca and Siberia is described. Transverse thin cell layer(tTCL)(1mm thick) explants of 'Casa Blanca' formed bulblets at a frequency of 97.7% when cultured on Murashige and Skoog (MS) medium supplemented with 1mg/L 2,4 dichlorophenoxyacetic acid(2,4-D) (On average 15.6 bulblets were formed per explant). The frequency of bulblet formation was drastically reduced when the explant ghickness was thinner than 1 mm. Explants from the outermost layer of bulb scale produced greater frequency of bulblet formation than middle or innermost layer. Among auxins supplemented to culture medium at 1 mg/L, 2,4-D led to greater frequency of bulblet formation on explants than dicamba, picamba, or phenylacetic acid(PAA). tTCL explants from the middle region of the outermost layer bulb scale yielded greater frequency of bulblet formation than the upper or lower region. tTCL stem explants of 'Siberia' formed bulblets at a frequency of 95.3% when cultured on MS medium with 1 mg/L 2,4-D(On average 9.1 bulblets were formed per explant). The system estabilished in this study will be useful for in vitro rapid propagation and genetic transformation of Lilium Oriental hybrids.

Effect of Planting Density, Growing Medium and Nutrient Solution Strength on Growth and Development of Lily in Box Culture (나리의 상자재배시 재식밀도, 배지 및 양액농도가 생육에 미치는 영향)

  • Chae, Soo Cheon
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.1
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    • pp.36-43
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    • 2008
  • This purpose of this study was to examine the effect of planting density, growing medium and strength of a nutrient solution (National Horticultural Research Institute's nutrient solution: HRI's) on the growth and development of Oriental hybrid lily 'Le Reve' in a box cultivation. The planting density with 14, 18 and 22 bulbs had sprouting one day earlier than other treatments. Planting density of 22 bulbs flowered first, while six bulbs flowered the last, indicating that higher planting densities led earlier flowering. The increasing planting density increased stem length of cut flowers. On the other hand, cut flower quality was improved when the planting density was lower. The incidence of physiological disorders such as blasting was more frequent in planting density of 22, 18, and 14, indicating that higher planting densities caused higher incidences of physiological disorders. All planting densities except 22 bulbs displayed superior results in width, weight, number, and scale weight of the bulbs. Greater planting densities led to inferior bulb enlargement and an increased decomposition rate. pH decreased in all treatments after the bulb enlargement and decreased more as the planting density increased. Contents of P, K, Ca, and Mg increased, while contents of K and Ca decreased, as the planting density increased. The rice hull+coir (1:1, v/v) treatment was better than others, but did not show that much of a difference. Moreover, in bulbs enlargement after cut flower harvest, lily medium and perlite+peat moss treatments showed superior results, and decomposition rate was the greatest in the rice hull+coir (1:1, v/v) treatment. In the HRI's solution strength treatment from the period of flower bud emergence to flower harvest, higher solution strengths gave better cut flower quality in terns of length, weight, and number of flowers. The non-treated control and one third strength of a HRI's solution hastened flowering, indicating that lower strengths led to earlier flowering. According to the results of leaf analysis as affected by solution strength during the flower harvest, absorption rates of N and K were greater when the strength was higher, and Ca and Mg showed the same tendency. On the other hand, the absorption rate of P was the lowest in all treatments.

Investigations on Lighting Environment of Living Room and Lighting Use According to the Behavior in Large Apartment Houses (대형 공동주택 거실 조명환경과 행위별 조명사용에 관한 조사연구)

  • Kim, Hyun-Ji;Kim, Hoon
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.26 no.3
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    • pp.1-8
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    • 2012
  • This study investigated current status of lighting environment, residents' awareness of lighting and lighting use according to behavior in the living room of apartment to determine current lighting trends and their related problems. The living rooms of newly-built apartment units(133~165[$m^2$] and larger than 166[$m^2$] within the past five years) were examined. The results are summarized as follows. 1. Resident satisfaction with current living room lighting was the highest in 'illumination' and the lowest was 'energy savings' regardless of the size of living room. 2. This kind of ceiling lighting layout, 133~165[$m^2$] unit used central lighting and larger than 166[$m^2$] units used central lighting and cove lighting. Regarding light sources, 133~165[$m^2$] linear FL, halogen, larger than 166[$m^2$] bulb-type FL are widely used. LED are now being found in the living room corridor and kitchen walls of newly-built apartment houses. 3. Watching TV and reading books were commonly behaved in the living room. Conversations between family, having desserts, resting, housekeeping, and entertaining were usually done weekdays, while having desserts and conversations between family were done weekends. Use of lighting as main behaviors is properly and differently required due to the distinct uses of lighting as residents' behaviors or their life style.

Study on Applicability of CGS Method based on Field Experiments and Cavity Expansion Theory (현장시험과 공동팽창이론을 통한 CGS 공법의 적용성 평가)

  • Jung, Hyun-Seok;Seo, Seok-Hyun;Choi, Hangseok;Lee, Hyobum
    • Journal of the Korean GEO-environmental Society
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    • v.20 no.2
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    • pp.19-28
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    • 2019
  • Grounds of the western coast of the Korean Peninsular are mostly composed of soft and cohesive soils, and it is necessary to carry out soil improvement before construction. The CGS (Compaction Grouting System) method has been commonly applied for the purpose of not only improving soft ground but also serving as the pile foundation of a bridge. In this paper, the CGS method was applied to the Incheon International Airport facility site, which consists of reclaimed landfill and soft clay soil, so as to evaluate the applicability of this soil improvement method to soft clay ground formations. Futhermore, results of construction were intensively studied along with a series of field experiments and theoretical consideration. The cone penetration tests were performed to assess the ground improvement effect of the CGS method. Consequently, the application of CGS method led to an increase in soil strength enough to be used as the pile foundation to support the bridge at the site. In addition, the size of the upper grout-bulb was estimated by adopting the cavity expansion theory and compared with that of actual grout bulb exhumed in the field. Therefore, it is proved that the cavity expansion theory can be utilized to predict and evaluate the improvement of soft ground.

Investigation of Physiological and Yield Responses to Temperature Increases in Northern-ecotype Garlic (Allium sativum L. ) 'Uiseong' in Temperature Gradient Tunnels (한지형 마늘 '의성'의 온도구배하우스내 온도상승에 따른 생육 및 생리장해 조사)

  • Byung-Hyuk Kim;Min-Seon Choi;Chun Hwan Kim;Minji Shin;Seong Eun Lee;Kyung Hwan Moon;Hyun-Hee Han
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.4
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    • pp.276-283
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    • 2023
  • Garlic (Allium sativum L.) is one of the most important vegetables used in various foods in Korea and many countries. The growth of garlic is influenced by various abiotic factors such as cultivation temperature, humidity, minimum temperature duration, and photoperiod. This study investigated the effects of increasing temperatures on the plant growth of the northern- ecotype garlic 'Uiseong' in a temperature gradient tunnel. As a result, temperature increase led to decreases in the bulb diameter, weight, and clove pieces of garlic. The rise of cultivation temperature increased the occurrence rate of incomplete bolting in the Northern-ecotype garlic 'Uiseong', resulting in decreases in productivity and a decrease in the yield of marketable garlic, indicating that temperature increases affect the development of garlic bulb formation. The findings of this study are expected to contribute as foundational data for understanding the growth responses of the northern-ecotype 'Uiseong' to increasing cultivation temperatures. The results of this study can be used to develop designing garlic growth models. In addition, the results of this study can improve understanding the interaction between increased temperature and garlic growth.

Flowering Control by Using Red Light of Chrysanthemum (적색광을 이용한 국화의 개화조절)

  • Hong, Seung-Chang;Kwon, Soon-Ik;Kim, Min-Kyeong;Chae, Mi-Jin;Jung, Goo-Bok;So, Kyu-Ho
    • Korean Journal of Environmental Agriculture
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    • v.32 no.2
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    • pp.123-127
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    • 2013
  • BACKGROUND: The incandescent bulb and compact fluorescent lamp are widely using as a light sources for daylength extension of chrysanthemum. But, these light sources consume a lot of electricity and have short longevity. A light-emitting diode (LED) is a semi conductor light source. LEDs have many advantages over incandescent light sources including lower energy consumption, longer lifetime. In this study, we investigated the intensity of red light to control flowering of chrysanthemum (Dendranthema grandiflorum cv. "Shinma") by using LEDs. METHODS AND RESULTS: The red (660 nm) and far-red (730 nm) light were irradiated subsequently to investigate photo-reversible flowering responses of chrysanthemum. The flowering of chrysanthemum was inhibited by night interruption with red light but subsequently irradiated far-red light induced the flowering of chrysanthemum. This photoreversibility, reversion of the inductive effect of a brief red light pulse by a subsequent far-red light pulse, is a property of photo responses regulated by the plant photoreceptor phytochrome B. Four different intensity of red light of 0.7, 1.4, 2.1, and $2.8{\mu}mol/m^2/s$ (PAR) were irradiated at growth room in order to determine the threshold for floral inhibition of chrysanthemum. Over $1.4{\mu}mol/m^2/s$ of the red lights irradiated chrysanthemums were not flowered. The plant length, fresh weight, number of leaves, and leaf area of chrysanthemum irradiated with red light were increased by 17%, 36%, 11%, and 48%, respectively, compared to those of compact fluorescent lamp. CONCLUSION(S): The red light and subsequential far-red light showed that the photoreversibility on flowering of chrysanthemum. The red light ($1.4{\mu}mol/m^2/s$ of red LEDs) and white light (50 Lux of compact fluorescent lamp) have the same effect on inhibition of flowering in chrysanthemum. Additionally, the red light increased the plant height and dry weight of chrysanthemum.

Odorant Receptors Containing Conserved Amino Acid Sequences in Transmembrane Domain 7 Display Distinct Expression Patterns in Mammalian Tissues

  • Ryu, Sang Eun;Shim, Tammy;Yi, Ju-Yeon;Kim, So Yeun;Park, Sun Hwa;Kim, Sung Won;Ronnett, Gabriele V.;Moon, Cheil
    • Molecules and Cells
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    • v.40 no.12
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    • pp.954-965
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    • 2017
  • Mammalian genomes are well established, and highly conserved regions within odorant receptors that are unique from other G-protein coupled receptors have been identified. Numerous functional studies have focused on specific conserved amino acids motifs; however, not all conserved motifs have been sufficiently characterized. Here, we identified a highly conserved 18 amino acid sequence motif within transmembrane domain seven (CAS-TM7) which was identified by aligning odorant receptor sequences. Next, we investigated the expression pattern and distribution of this conserved amino acid motif among a broad range of odorant receptors. To examine the localization of odorant receptor proteins, we used a sequence-specific peptide antibody against CAS-TM7 which is specific to odorant receptors across species. The specificity of this peptide antibody in recognizing odorant receptors has been confirmed in a heterologous in vitro system and a rat-based in vivo system. The CAS-TM7 odorant receptors localized with distinct patterns at each region of the olfactory epithelium; septum, endoturbinate and ectoturbinate. To our great interests, we found that the CAS-TM7 odorant receptors are primarily localized to the dorsal region of the olfactory bulb, coinciding with olfactory epithelium-based patterns. Also, these odorant receptors were ectopically expressed in the various non-olfactory tissues in an evolutionary constrained manner between human and rats. This study has characterized the expression patterns of odorant receptors containing particular amino acid motif in transmembrane domain 7, and which led to an intriguing possibility that the conserved motif of odorant receptors can play critical roles in other physiological functions as well as olfaction.

The Verification of Photoplethysmography Using Green Light that Influenced by Ambient Light (녹색광을 이용한 반사형 광용적맥파측정기의 주변광 간섭시 신호측정)

  • Chang, K.Y.;Ko, H.C.;Lee, J.J.;Yoon, Young Ro
    • Journal of Biomedical Engineering Research
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    • v.35 no.5
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    • pp.125-131
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    • 2014
  • The purpose of this study is to verify the utility of reflected photoplethysmography sensor using two green light emitting diodes that influenced by ambient light. Recently it has been studied that green light emitting diode is suitable for light source of reflected photoplethysmography sensor at low temperature and high temperature. Another study showed that, green light is better for monitoring heart rate during motion than led light. However, it has a bad characteristic about ambient light noise. To verify the utility of reflected photoplethysmography sensor using green light emitting diode, this study measures the photoplethysmography signal that is distorted by ambient light and will propose a solution. This study has two parts of research method. One is measurement system that composed sensor and board. The sensor is made up PE-foam and Non-woven fabric for flexible sensor. The photoplethysmography signal is measured by measurement board that composed high-pass filter, low-pass filter and amplifier. Ambient light source is light bulb and white light emitting diode that has three steps brightness. Photoplethysmography signal is measured with lead II electrocardiography signal at the same time and it is measured at the finger and radial artery for 1 minute, 1000 Hz sampling rate. The lead II electrocardiography signal is a standard signal for heart rate and photoplethysmography signal that measured at the finger is a standard signal for waveform. The test is repeated 3 times using three sensor. The data is processed by MATLAB to verify the utility by comparing the correlation coefficient score and heart rate. The photoplethysmography sensor using two green light emitting diodes is shown better utility than using one green light emitting diode and red light emitting diode at the ambient light. The waveform and heart rate that measured by two green light emitting diodes are more identical than others. The amount of electricity used is less than red light emitting diode and error peak detectability factor is the lowest.

Effect of Photoperiod Considering Animal Welfare for Growth Ability and Blood Properties in Broiler Chicks (동물복지를 고려한 점등시간이 육계 병아리의 성장 능력 및 혈액 성상에 미치는 영향)

  • Kim, Hee-Jin;Son, Jiseon;Jeon, Jin-Joo;Kim, Hyun-Soo;Kang, Hwan-Ku;Lee, Woo-Do;Yun, Yeon-Seo;Hong, Eui-Chul
    • Korean Journal of Poultry Science
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    • v.49 no.3
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    • pp.139-144
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    • 2022
  • This study was conducted to investigate the effect of three different photoperiods on growth performance, blood properties, and stress indicators in broiler chicks between 1-7 days after hatching. Two hundred and fifty-two 1-day-old male broiler chicks (57.0±0.12 g) were divided into three treatments, with 4 replicates per treatment and 22 birds per replicate subjected to three different photoperiods of 24L, 22L/2D and 18L/6D. A light-emitting diode bulb served as the light source, with an illuminance of 30 lx. As an experimental diet, a commercial feed based on a corn-soybean meal, with 22% CP and 3,150 kcal/kg ME diet, and water were fed ad libitum. Body weight gain, feed conversion ratio, and liver weight ratio showed a statistically significant difference between the 18L/6D and 24L treatments (P<0.05), but with no significant difference between the 22L/2D treatment and either the 24L or 18L/6D treatment. The breast meat ratio was 5.59% in the 18L/6D treatment group, which was lower than that of other treatment groups (P<0.05). The triglyceride levels were highest (P<0.05) in the 18L/6D treatment among treatments, but alanine aminotransferase levels were significantly higher (P<0.05) in the 22L/2D treatment than in the 24L treatment. Levels of cytokines, i.e., Interleukin-6 and Tumor Necrosis Factor-α did not show a significant difference among the treatments, but corticosterone content was significantly higher (P<0.05) in the 24L treatment than in the 18L/6D treatment. In conclusion, 22 hours of lighting is appropriate between 1~7 days after hatching, considering growth performance and the overall health of broiler chicks.