• Title/Summary/Keyword: LC

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Amorphopallus Paeoniifolius in Greenhouse Environment: Leaf Cycle (인공환경 생육조건에서의 Amorphopallus paeoniifolius: Leaf Cycle)

  • Ahn, TaiHyeon;Goh, YeoBin;Bae, JunKyu;Lee, JeongHo;Lee, KiCheol
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.48-48
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    • 2019
  • Amorphophallus paeoniifolius (Dennst.) Nicolson는 영명으로 Elephant yam 또는 Whitespot giant arum 으로 불리는 천남성과(Araceae) 식물로 동아시아, 뉴기니, 오세아니아, 마다가스카르 등지에서 자생하고 있으며 숲의 가장자리 또는 2차림에서 발견 된다. 다년생 식물로 덩이줄기는 어두운 갈색의 둥근모양으로 직경 20~25 cm로 자라고 수염뿌리가 사방으로 달린다. IUCN 적색목록(Red List)에 관심대상 종(LC: Least Concern)에 속하는 식물이다. 현지인들은 마(Dioscorea Polystachya Turcz.)처럼 채소로 먹기도 하며 약용으로 쓰이기도 하는데 복부장애, 소화불량, 천식, 기관지염, 빈혈 등에 약효가 있다. Amorphophallus paeoniifolius은 $25^{\circ}C{\sim}35^{\circ}C$, 연강수량 1,000~1,500 mm에서 잘 자란다. 가운데 눈이 올라오면서 생장을 시작하고 며칠이 지나서야 잎 또는 꽃으로 자라는지 알 수 있다. 잎의 생육주기는 잎눈이 생장을 시작하면 잎자루 끝에 소엽과 소잎자루가 접힌 채로 올라온다. 소엽들이 펴지면서 완전한 모습을 갖추는데 30일 이상의 생장기간을 갖는다. 잎의 형태는 우상복엽(pinnate compound leaf)으로 우산처럼 보이는 잎 하나로 광합성을 한다. 잎은 최대 높이 2.5~3 m, 너비 3 m까지 자라며, 잎자루의 색은 녹색과 청색으로 얼룩덜룩한 무늬가 있다. 인공환경 조건에서 Amorphophallus paeoniifolius 생활사 중 잎의 주기를 연구하기 위해서 광, 온도 등의 지상부 환경은 열대 및 아열대 식물의 자생지와 유사하게 조성하였고, 지하부 환경은 인공 배합토를 사용하여 조성하였다. 평균온도는 $25{\sim}28^{\circ}C$, 겨울철 최저 $16^{\circ}C$, 여름철 최고 $33^{\circ}C$를 유지 관리 하였다. 자동 환경제어시스템으로 온도 및 환기를 유지 관리하고 필요에 따라 수동제어 관리를 병행하여 조절하였다. Amorphophallus paeoniifolius는 잎을 먼저 생성하고 광합성으로 생산된 영양물질을 덩이뿌리에 저장을 하고 그 영양물질을 이용하여 꽃을 피우는 생육 특성을 지닌다. 실험에 사용된 공시 식물은 2018년 12월 미얀마에서 생체(덩이줄기) 형태로 도입되었다. 화분에 식재 후 약 5개월이 지난 시점에서 잎의 생장이 시작되었다. 2019년 7월 29일 기준으로 높이 80 cm, 너비 60 cm의 크기로 성장하였으며, 생육환경에 따라 3~5개월 뒤 잎이 지고 나면 다시 덩이줄기로 되돌아갈 것으로 판단된다. 하지만 지금까지 이 식물에 알려진 정보는 인공환경에서의 연구가 아닌 자연환경에서의 연구결과이기 때문에 인공적인 온실 환경에서 자란 Amorphophallus paeoniifolius는 잎의 주기는 더 오래 갈 수도 있으며 꽃의 주기 또한 느리거나 빠를 수 있다. 잎의 생장주기(Leaf Cycle)시 잎자루가 낮과 밤의 방향을 달리 하여 자라는 것이 관찰되었다. 이는 광합성을 위해서 잎자루의 방향을 햇빛 방향으로 돌리는 것으로 판단된다. Amorphophallus paeoniifolius를 실내 조경 식물 또는 식 약용의 소재식물로 활용하기 위해서는 꽃의 생장주기 등 추가적인 모니터링과 연구가 필요하다.

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Effects of Heavy Metal Concentrations on Seed Germination and Rhizome Development of Campanula takesimana Nakai (중금속 농도가 섬초롱꽃 종자 발아 및 유근 발달에 미치는 영향)

  • Kim, Tae Hyun;Kim, Do Hyun;Im, Hyeon Jeong;Choi, Kyu Seung;Kim, Sang Geun;Song, Chi Hyeon;Oh, Beom Seok;Kim, Yang Su;Song, Ki Seon;Won, Chang O
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.39-39
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    • 2019
  • 섬초롱꽃은 울릉군에 한정적으로 분포하는 여러해살이풀이다. 산림청에서는 약관심종(LC) 등급의 희귀식물이자 특산식물로 분류하고 있으며, 꽃의 관상가치가 특히 높아 화단용 소재, 절화용, 화분재배 등으로 흔히 이용되고 있다. 따라서 본 연구는 섬초롱꽃의 대량증식 시, 중금속이 미칠 수 있는 영향에 대해 파악하고자 수행하였다. 실험에 사용한 중금속은 카드뮴(Cd), 철(Fe) 두 종류이며, 두 종류 모두 1% 한천(agar)배지에 10, 25, 50, 100, 200 mg/L 농도로 녹여 사용하였다. 발아율 및 유근 생장을 위해 종자 및 유묘를 치상 후, $25^{\circ}C$ 항온 생장상에서 발아율 및 유근 발달을 관찰하였다. 발아율 측정결과, 아무처리도하지 않은 1% 한천(agar) 배지에서 $62.7{\pm}7.4%$로 가장 높았고 중금속 농도가 높아질수록 감소하였다. 카드뮴(Cd) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 $17.3{\pm}1.3$, $2.7{\pm}1.3$, $1.3{\pm}1.3$, 0, 0%로 나타났다. 한편, 철(Fe) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 $46.7{\pm}4.8$, $24.0{\pm}2.3$, $36.0{\pm}6.9$, $20.0{\pm}2.3$, 0%로 나타났다. 유근의 평균 생장 길이(mm)는 아무처리도 하지 않은 1% 한천(agar) 배지에서 19.6 mm로 가장 높았고, 중금속 농도가 높아질수록 낮게 나타났다. 카드뮴(Cd) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 4.6 mm, 1.9 mm, 0.7 mm, 0.9 mm, 0 mm 생장한 것으로 나타났다. 한편, 철(Fe) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 15.9 mm, 8.6 mm, 8.1 mm, 0.9 mm, 0 mm 생장한 것으로 나타났다. 실험결과를 종합해 볼 때, 섬초롱꽃 종자 발아 및 유근 생장의 억제는 카드뮴(Cd) 처리구에서 높게 나타났고, 철(Fe) 처리구에서는 비교적 영향을 덜 미치는 것으로 나타났다. 특히 카드뮴은 10 mg/L 농도만 처리하더라도 약 3.6~4.2배의 발아율 생장율 감소가 나타나, 섬초롱꽃 대량증식 시, 치명적일 것으로 판단된다. 본 실험의 결과는 섬초롱꽃 종자를 이용한 대량증식 및 유묘재배에 있어, 토양을 선택하는데 중요한 기초자료로서 활용될 것으로 기대된다.

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Deciphering the role of a membrane-targeting domain in assisting endosomal and autophagic membrane localization of a RavZ protein catalytic domain

  • Park, Jui-Hee;Lee, Seung-Hwan;Park, Sang-Won;Jun, Yong-Woo;Kim, Kunhyung;Jeon, Pureum;Kim, Myungjin;Lee, Jin-A;Jang, Deok-Jin
    • BMB Reports
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    • v.54 no.2
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    • pp.118-123
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    • 2021
  • The bacterial effector protein RavZ from a pathogen can impair autophagy in the host by delipidating the mammalian autophagy-related gene 8 (mATG8)-phosphatidylethanolamine (PE) on autophagic membranes. In RavZ, the membrane-targeting (MT) domain is an essential function. However, the molecular mechanism of this domain in regulating the intracellular localization of RavZ in cells is unclear. In this study, we found that the fusion of the green fluorescent protein (GFP) to the MT domain of RavZ (GFP-MT) resulted in localization primarily to the cytosol and nucleus, whereas the GFP-fused duplicated-MT domain (GFP-2xMT) localized to Rab5- or Rab7-positive endosomes. Similarly, GFP fusion to the catalytic domain (CA) of RavZ (GFP-CA) resulted in localization primarily to the cytosol and nucleus, even in autophagy-induced cells. However, by adding the MT domain to GFP-CA (GFP-CA-MT), the cooperation of MT and CA led to localization on the Rab5-positive endosomal membranes in a wortmannin-sensitive manner under nutrient-rich conditions, and to autophagic membranes in autophagy-induced cells. In autophagic membranes, GFP-CA-MT delipidated overexpressed or endogenous mATG8-PE. Furthermore, GFP-CA△α3-MT, an α3 helix deletion within the CA domain, failed to localize to the endosomal or autophagic membranes and could not delipidate overexpressed mATG8-PE. Thus, the CA or MT domain alone is insufficient for stable membrane localization in cells, but the cooperation of MT and CA leads to localization to the endosomal and autophagic membranes. In autophagic membranes, the CA domain can delipidate mATG8-PE without requiring substrate recognition mediated by LC3-interacting region (LIR) motifs.

Uptake and Distribution of Bisphenol A and Its Metabolites in Lettuce Grown in Sandy Loam and Loam Soil

  • Cho, Il Kyu;Jeon, Yong-Bae;Oh, Young Goun;Rahman, Md. Musfiqur;Kim, Won-Il;Lee, Young-Deuk
    • Korean Journal of Environmental Agriculture
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    • v.39 no.4
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    • pp.375-383
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    • 2020
  • BACKGROUND: Bisphenol A (BPA) is a chemical widely used in polycarbonate plastics, epoxy resins. BPA is an endocrine disruptor. Residue of BPA in agricultural environments is a major concern. The objective of this study was to understand the characteristics of the uptake and distribution of BPA and its metabolites introduced into the agricultural environment to crops, and to use it as basic data for further research on reduction of BPA in agricultural products. METHODS AND RESULTS: This study established the analysis method of BPA and its metabolites in soil and crops, and estimated the intake of BPA and its metabolites from lettuce (Lactuca sativa) grown in sandy loam and loam soil, which are representative soils in Korea. The two major metabolites of BPA were 4-hydroxyacetophenone (4-HAP) and 4-hydroxybenzoic acid (4-HBA). BPA, 4-HAP and 4-HBA have been analyzed by using liquid chromatography tandem mass spectrometry (LC-MS/MS). These substances were detected in sandy loam and loam soil, indicating that certain portions of BPA were converted to 4-HAP and 4-HBA in the soil; however, it was observed that only 4-HBA migrated to lettuce through the roots into crops. CONCLUSION: The uptake residues showed the BPA and 4-HAP were not detected in lettuces grown on sandy loam (SL) and loam (L) soil treatments that were applied with of 10 ng/g, 50 ng/kg and 500 ng/g of BPA. However, the 4-HBA was detected at the level of 7 ng/g and 11 ng/g in the lettuce grown in sandy loam and loam soil that were treated with the 500 ng/g of BPA, respectively, while the 8 ng/g of 4-HBA was measured in the lettuce cultivated in the loam that was treated with 100 ng/g of BPA. This result presents that the BPA persisting in the soil of the pot was absorbed through the lettuce roots and then distributed in the lettuce leaves at the converted form of 4-HBA, what is the oxidative metabolite of BPA.

A Case Report of Korean Medicine Treatment of Non-small Cell Lung Cancer with Pleural Metastasis Following Targeted Chemotherapy with Afatinib (Afatinib 표적항암치료를 시행 중인 흉막 전이된 비소세포폐암 환자의 한방치료 증례보고)

  • Kim, Kyun Ha;Kang, Hee-kyung;Kim, So-yeon;Han, Chang-woo;Park, Seong-ha;Yun, Young-ju;Lee, In;Kwon, Jung-nam;Hong, Jin-woo;Choi, Jun-Yong
    • The Journal of Internal Korean Medicine
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    • v.41 no.6
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    • pp.1255-1264
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    • 2020
  • Objective: The purpose of this study was to report the effect of Korean medicine on a patient with non-small cell lung cancer with pleural metastasis who had been treated with afatinib. Method: A 61-year old female patient with non-small cell lung cancer with pleural metastasis was treated with acupuncture and herbal medicines, including Yijung-tang, Haengso-tang, Samchulkunbi-tang, Paeamju-bang (Feiai zhu fang), to control various symptoms caused by afatinib. The degree of pain was assessed by a numeric rating scale (NRS) and the quality of life was determined with the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire, Core 30 (EORTC QLQ-C30) and the EORTC 13-item lung cancer-specific module (EORTC LC-13). Results: After receiving acupuncture and herbal medicines, the patient showed improvement in her back and chest pain, according to the NRS score. Similarly, the Korean medicinal treatments significantly relieved her nausea, vomiting, diarrhea, hemoptysis, and alopecia. However, the EORTC QLQ-C30 assessment suggested that Korean medicinal treatments did not significantly improve the global health status of this patient. Conclusion: Korean medicine could be useful in relieving some of the symptoms occurring after conventional afatinib treatments.

Cellular-protective effects of Nardotidis seu Sulculii Concha Extract against oxidative stress (산화적 스트레스에 대한 석결명의 세포 보호 효과)

  • Kim, Kwang Yeon;Lee, Seung Jin;Jee, Seon Young;Bae, Su Jin;Song, Yu Rim;Yun, Un-Jung;Bak, Seonbeen;Song, Jong Kuk;Son, Tae Jin;Son, Jae-Dong;Kim, Woo Hyun;Yang, Ju Hye;Park, Sun Dong;Kim, Sang Chan;Kim, Young Woo;Park, Kwang-Il
    • Herbal Formula Science
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    • v.29 no.2
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    • pp.71-80
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    • 2021
  • Objectives : This study investigated cellular-protective effects of Nardotidis seu Sulculii Concha water extract (NSCE) against oxidative stress induced by arachidonic acid (AA)+iron or tert-butylhydroperoxide (tBHP). Methods : In vitro, MTT assay was assessed for cell viability, and immunoblotting analysis was performed to detect expression of AMP-activated kinase (AMPK) signaling pathway and autophagy related proteins. In vivo, mice were orally administrated with the aqueous extract of NSCE of 500 mg/kg for 3 days, and then injected with CCl4 0.5 mg/kg body weight to induce acute damage. The level of liver damage was measured by serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) analysis. Results : Treatment with NSCE inhibited cell death induced by AA+iron and tBHP. NSCE induced the phosphorylation of AMPK, and this compound also induced the phosphorylation of LKB1, an upstream kinase of AMPK, and Acetyl-CoA carboxylase (ACC), a primary downstream target of AMPK. NSCE increased the protein levels of autophagic markers (LC3II and beclin-1) and decreased the phosphorylation of mammalian target of rapamycin (mTOR) and simultaneously increased the phosphorylation of unc-51-like kinase-1 (ULK-1) in time-dependent manner. Conclusions : NSCE has the ability 1) to protect cells against oxidative stress induced by AA+iron or tBHP. NSCE 2) to activate AMP-activated protein kinase (AMPK), and 3) to regulate autophagy, an important regulator in cell survival.

Gintonin facilitates brain delivery of donepezil, a therapeutic drug for Alzheimer disease, through lysophosphatidic acid 1/3 and vascular endothelial growth factor receptors

  • Choi, Sun-Hye;Lee, Na-Eun;Cho, Hee-Jung;Lee, Ra Mi;Rhim, Hyewhon;Kim, Hyoung-Chun;Han, Mun;Lee, Eun-Hee;Park, Juyoung;Kim, Jeong Nam;Kim, Byung Joo;Nah, Seung-Yeol
    • Journal of Ginseng Research
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    • v.45 no.2
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    • pp.264-272
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    • 2021
  • Background: Gintonin is a ginseng-derived exogenous G-protein-coupled lysophosphatidic acid (LPA) receptor ligand, which exhibits in vitro and in vivo functions against Alzheimer disease (AD) through lysophosphatidic acid 1/3 receptors. A recent study demonstrated that systemic treatment with gintonin enhances paracellular permeability of the blood-brain barrier (BBB) through the LPA1/3 receptor. However, little is known about whether gintonin can enhance brain delivery of donepezil (DPZ) (Aricept), which is a representative cognition-improving drug used in AD clinics. In the present study, we examined whether systemic administration of gintonin can stimulate brain delivery of DPZ. Methods: We administered gintonin and DPZ alone or coadministered gintonin with DPZ intravenously or orally to rats. Then we collected the cerebral spinal fluid (CSF) and serum and determined the DPZ concentration through liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Results: Intravenous, but not oral, coadministration of gintonin with DPZ increased the CSF concentration of DPZ in a concentration- and time-dependent manner. Gintonin-mediated enhancement of brain delivery of DPZ was blocked by Ki16425, a LPA1/3 receptor antagonist. Coadministration of vascular endothelial growth factor (VEGF) + gintonin with DPZ similarly increased CSF DPZ concentration. However, gintonin-mediated enhancement of brain delivery of DPZ was blocked by axitinip, a VEGF receptor antagonist. Mannitol, a BBB disrupting agent that increases the BBB permeability, enhanced gintonin-mediated enhancement of brain delivery of DPZ. Conclusions: We found that intravenous, but not oral, coadministration of gintonin facilitates brain delivery of DPZ from plasma via LPA1/3 and VEGF receptors. Gintonin is a potential candidate as a ginseng-derived novel agent for the brain delivery of DPZ for treatment of patients with AD.

Rapid separation of Capsicum annuum L. leaf extract using automated HPLC/SPE/HPLC coupling system (Sepbox system) and identification of α-glucosidase inhibitory active substances (자동화 HPLC/SPE/HPLC 시스템(Sepbox system)을 활용한 고추 잎 (leaf of Capsicum annuum L.) 추출물 분리 및 α-glucosidase 억제 활성 물질 탐색)

  • Kim, Min-Seon;Jin, Jong Beom;Lee, Jung Hwan;An, Hye Suck;Pan, Cheol-Ho;Park, Jin-Soo
    • Journal of Applied Biological Chemistry
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    • v.64 no.1
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    • pp.25-32
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    • 2021
  • Phytochemicals include plant-derived natural products that promote and improve the human metabolism and physiological activity, and there is a lot of research to find the value of the molecules is in progress. Likewise, we obtained 288 fractions of Capsicum annuum L. extract in less than 20 h using HPLC/SPE/HPLC coupling experiment through Sepbox system, an effective separation system to search for active substances in natural resources and ensure efficacy and reliability. Therefore, this experiment allowed rapid identification of biologically active molecules from the extract compared to traditional separation processes. Of the above fractions, eight fractions showed the α-glucosidase inhibitory (AGI) activity and subsequent LC-MS analysis revealed one of the active molecules as luteolin 7-O-glucoside. In addition, we proved the increase in AGI activity according to deglycosylation of flavonoid glycoside. Therefore, this study suggests that the Sepbox system can quickly separate and identify active components from plant extract, and is an effective technique for finding new active substances.

Status of Groundwater Potential Mapping Research Using GIS and Machine Learning (GIS와 기계학습을 이용한 지하수 가능성도 작성 연구 현황)

  • Lee, Saro;Fetemeh, Rezaie
    • Korean Journal of Remote Sensing
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    • v.36 no.6_1
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    • pp.1277-1290
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    • 2020
  • Water resources which is formed of surface and groundwater, are considered as one of the pivotal natural resources worldwide. Since last century, the rapid population growth as well as accelerated industrialization and explosive urbanization lead to boost demand for groundwater for domestic, industrial and agricultural use. In fact, better management of groundwater can play crucial role in sustainable development; therefore, determining accurate location of groundwater based groundwater potential mapping is indispensable. In recent years, integration of machine learning techniques, Geographical Information System (GIS) and Remote Sensing (RS) are popular and effective methods employed for groundwater potential mapping. For determining the status of the integrated approach, a systematic review of 94 directly relevant papers were carried out over the six previous years (2015-2020). According to the literature review, the number of studies published annually increased rapidly over time. The total study area spanned 15 countries, and 85.1% of studies focused on Iran, India, China, South Korea, and Iraq. 20 variables were found to be frequently involved in groundwater potential investigations, of which 9 factors are almost always present namely slope, lithology (geology), land use/land cover (LU/LC), drainage/river density, altitude (elevation), topographic wetness index (TWI), distance from river, rainfall, and aspect. The data integration was carried random forest, support vector machine and boost regression tree among the machine learning techniques. Our study shows that for optimal results, groundwater mapping must be used as a tool to complement field work, rather than a low-cost substitute. Consequently, more study should be conducted to enhance the generalization and precision of groundwater potential map.

Analysis of antioxidant and anti-inflammatory effects of Mongolian wild lingonberry and blueberry, and identification of their bioactive compounds (몽골 야생 링곤베리와 블루베리의 항산화, 항염증 효과 및 생리활성 물질 분석 연구)

  • Lee, Hye Ju;Naranbulag, Batdorj;Jeong, Seung Jin;Seo, Chan;Lee, Sang-Gil
    • Korean Journal of Food Science and Technology
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    • v.54 no.2
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    • pp.147-154
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    • 2022
  • The Mongolian lingonberry and blueberry are two essential food sources found in Mongolia. This study investigated the antioxidant and anti-inflammatory effects of methanol extracts from Mongolian lingonberry (LBE) and blueberry (BBE). Compared to the LBE, the BBE showed higher total phenolic, flavonoid, and anthocyanin contents, as well as antioxidant capacities. The LBE and BBE inhibited the mRNA expression of pro-inflammatory genes, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and cyclooxygenase (COX-2) in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. In addition, the LBE and BBE inhibited NADPH oxidase-2 (Nox2) mRNA expression, indicating that they have cellular antioxidant capacities. Anthocyanin derivatives of the LBE and BBE were analyzed using LC-QTOF/MS. Six anthocyanins were identified in the BBE, while one was detected in the LBE. Our findings demonstrate that the anthocyanin-rich LBE and BBE could be used as functional food sources in Mongolia.