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http://dx.doi.org/10.3839/jabc.2021.005

Rapid separation of Capsicum annuum L. leaf extract using automated HPLC/SPE/HPLC coupling system (Sepbox system) and identification of α-glucosidase inhibitory active substances  

Kim, Min-Seon (Natural Product Informatics Research Center, Korea Institute of Science and Technology)
Jin, Jong Beom (Natural Product Informatics Research Center, Korea Institute of Science and Technology)
Lee, Jung Hwan (Natural Product Informatics Research Center, Korea Institute of Science and Technology)
An, Hye Suck (Marine Biology Research Division, National Marine Biodiversity Institute of Korea)
Pan, Cheol-Ho (Natural Product Informatics Research Center, Korea Institute of Science and Technology)
Park, Jin-Soo (Natural Product Informatics Research Center, Korea Institute of Science and Technology)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.1, 2021 , pp. 25-32 More about this Journal
Abstract
Phytochemicals include plant-derived natural products that promote and improve the human metabolism and physiological activity, and there is a lot of research to find the value of the molecules is in progress. Likewise, we obtained 288 fractions of Capsicum annuum L. extract in less than 20 h using HPLC/SPE/HPLC coupling experiment through Sepbox system, an effective separation system to search for active substances in natural resources and ensure efficacy and reliability. Therefore, this experiment allowed rapid identification of biologically active molecules from the extract compared to traditional separation processes. Of the above fractions, eight fractions showed the α-glucosidase inhibitory (AGI) activity and subsequent LC-MS analysis revealed one of the active molecules as luteolin 7-O-glucoside. In addition, we proved the increase in AGI activity according to deglycosylation of flavonoid glycoside. Therefore, this study suggests that the Sepbox system can quickly separate and identify active components from plant extract, and is an effective technique for finding new active substances.
Keywords
${\alpha}$-glucosidase inhibitory activity; Capsicum annuum L.; Deglycosylation; Sepbox system;
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Times Cited By KSCI : 1  (Citation Analysis)
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