• Asian-Australasian Journal of Animal Sciences
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• v.26 no.7
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• pp.971-980
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• 2013

Dried stoned olive pomace (DSOP) was administered to dairy water buffaloes, and their productive performance and milk composition were analysed. Sixteen pluriparous lactating buffaloes were divided into two uniform groups (control and experimental), taking into consideration the following parameters: milk production (2,192 and 2,102 kg) and duration of lactation (254 and 252 d) of the previous year, distance from calving (51 and 43 d), milk production (9.71 and 10.18 kg/d), body condition score (BCS) (6.44 and 6.31) and weight (617 and 653 kg) at the beginning of the trial. Both diets had the same formulation: second cut alfalfa hay 20%, corn silage 42%, concentrate 38% but the two concentrates differed in their formulation, the experimental one contained 15.50% of DSOP as fed. The employed DSOP showed high amounts of secoiridoids, such as 3,4-dihydroxyphenylethanol (3,4-DHPEA) (1.2 g/kg DM), 3,4-dihydroxyphenylethanol-elenolic acid di-aldehyde (3,4-DHPEA-EDA) (12.6 g/kg DM), p-hydroxyphenylethanol-elenolic acid di-aldehyde (p-HPEA-EDA) (5.6 g/kg DM) and lignans, which are known to be powerful bioactive compounds. The control diet had an energy-protein content of 0.86 Milk FU/kg DM and 143.3 g/kg DM of crude protein, whereas the experimental diet of 0.87 Milk FU/kg DM and 146.6 g/kg DM of crude protein. Each animal of the two groups received 17 kg DM/d and each buffalo of the experimental group, by way of the concentrate, ingested 1.05 kg DM/d of DSOP. The trial lasted 40 days. No significant difference was found between the BCS (6.41 and 6.53), live weight (625.93 and 662.50 kg) and milk production (9.69 and 10.08 kg/d) of the two groups, as was the case for fat, protein, lactose, pH and coagulating parameters of the two milks. The milk fat of the experimental group had a significantly higher content of total tocopherols (10.45 vs $8.60{\mu}g/g$, p<0.01) and retinol (3.17 vs $2.54{\mu}g/g$, p<0.01). The content of the reactive substances with tiobarbituric acid (TBARs) was significantly lower in the milk fat of the experimental group (12.09 vs $15.05{\mu}g$ MDA/g, p<0.01). The acid content of the milk fat of the experimental group had a significantly higher content (p<0.05) of C18:0 and of $C18:3{\omega}6$. LC-MS/MS analysis showed the presence of 3,4-DHPEA ($36.0{\mu}g/L$) in the milk of the DSOP-fed buffaloes, while other phenols were not found. DSOP, in the quantity utilized, can be used in the feeding of the lactating buffalo; the dietetic-nutritional characteristics of the milk are improved due to a greater contribution of tocopherols, retinol and the presence of hydroxytyrosol.

• Journal of Mushroom
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• v.12 no.4
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• pp.357-362
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• 2014

Pathogenicity of Steinernema carpocapsae GSN1 strain was evaluated against Morophagoides moriutii larvae. In Petridish tests, insect mortality by the nematode was dose dependent, which increased with dose from 5 to 160 infective juveniles(IJs)/larva. Pathogenicity against fourth-instar larvae was higher than the rate of corresponding second- and third-instar larvae, showing 100% insect mortality with the dose of 40 IJs/$4^{th}$ instar larva and 80 IJs/$2^{nd}$ or $3^{rd}$ instar larvae. Lethal concentration values at 50% ($LC_{50}$) of S. carpocapsae GSN1 strain were 4.2 IJs/$2^{nd}$ instar larva; 8.5 IJs/$3^{rd}$ instar larva; and 2.3 IJs/$4^{th}$ instar larva, respectively. The number of nematodes established in M. moriutii larvae after infection increased in the increment of dose and insect developmental stage. The highest number of nematodes was harvested from fourth instar larvae of M. moriutii at a dose of 160 IJs per larva, showing 22.5 nematodes per insect larva. Nematode reproductive capacity was related to insect developmental stage, showing 6,335 IJs/$2^{nd}$ instar larva, 21,660 IJs/$3^{rd}$ instar larvae, and 88,700 IJs/$4^{th}$ instar larvae.

• Journal of the korean academy of Pediatric Dentistry
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• v.36 no.3
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• pp.427-432
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• 2009

The aim of this study was to compare the shear-peel strength and the fracture site of 5 commercially available orthodontic band cements. One hundred molar bands were cemented to extracted human 3rd molars. The specimens were prepared in accordance with the manufacturer's instructions for each cement. After storage in a humidor at $37^{\circ}C$ for 24 hours, the shear debonding force was assessed for each specimen using an universal testing machine with crosshead speed of 2 mm/minute. Maximal failure stress was converted to mean shear-peel strength, MPa. The predominant site of band failure was recorded visually for all specimens as either at the band/cement or cement/enamel interface. Mean shear-peel strength of Ormco was the highest(2.44${\pm}$0.57), followed by Fuji $Ortho^{TM}$(2.24${\pm}$0.50), $Ketac-Cem^{TM}$(2.10${\pm}$0.57), 3M $Unitek^{TM}$(1.82${\pm}$0.43), $Band-Lok^{TM}$(1.73${\pm}$0.28). There were statistically significant differences between Ormco and $Band-Lok^{TM}$, Ormco and 3M $Unitek^{TM}$, and Fuji $Ortho^{TM}$ and $Band-Lok^{TM}$(p<0.05). The predominant site of bonding failure for bands cemented with the Ormco was at the band/cement interface, whereas bands cemented with Ultra $Band-Lok^{TM}$ failed predominantly at the enamel/cement interface. There was no significant difference among the other cements(Fuji $Ortho^{TM}$, 3M $Unitek^{TM}$, $Ketac-Cem^{TM}$).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.3
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• pp.496-504
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• 1997

This study was carried out to estimate toxic effects of phenol on survival and metabolism of the abalone juvenile, Haliotis discus hannai. The experiment was conducted by renewal bioassay procedure with different salinities at $20^{\circ}C$. The $LC_{50}$ of the juvenile exposed to phenol in the range of 0.5 and $100mg/\ell\;was\;34.3\~6.5mg/\ell\;at\;2.4\%_{\circ}\;and\;52.2\~9.3m/\ell\;at\;32\%_{\circ}$ salinity with exposure time from 24 hours to 96 hours. $LT_{50}$ was remarkablely reduced with increase of phenol conentration and decrease of salinity. Lethal toxicity or phenol was higher at low salinity than at high salinity. Therefore, salinity is likely to be one of factor to increase phenol toxicity. The oxygen consumption of the juvenile was reduced with increase of phenol concentration and with decrease of salinity. In spite of phenol toxicity, the oxygen consumption of the juvenile exposed to phenol of low concentration was high and similar as compared with that of control group. Survival rates of the abalone kept in phenol-free sea water after exposure to phenol concentration of 5, 10 and $20mg/\ell$ for 96 hours were reduced with decrease of salinity. Durations required to recover the normal metabolic rate of the juvenile, which was exposed to phenol concentration of 5, 10 and $20mg/\ell$ for 96 hours, were made longer with increasing phenol concentration. In the case of the juvenile exposed to sublethal concentration of phenol for 15 days, it were elongated as compared with that of the abalone exposed to phenol concentration caused acute toxicity. The result of this experiment indicated that relatively low concentration of phenol can impact on the abalone juvenile in marine ecosystem.

• Korean Journal of Plant Resources
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• v.36 no.2
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• pp.107-121
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• 2023

This study analyzed the polyphenol, flavonoid contents, antioxidant activity, anti-aging activity and phenol component contents of Saccharina japonica (SJ), Costaria costata (CC) extracts with hot water, 95% methanol, 95% prethanol for investigating possible utilization of SJ and CC extracts. The result revealed that the SJ and CC methanol extracts showed the highest polyphenol and flavonoid contents, 4.63 mg TAN/g, and 4.19 mg QUE/g respectively. Also, the SJ and CC methanol extracts showed higher antioxidant activity than prethanol and hot water extracts, whereas the ABTS radical scavenging activities were the highest in prethanol extracts (IC₅₀ = 15.4, 10.3 ㎍/µL). In anti-aging activity for evaluating the anti-wrinkle activity and skin whitening activity, the CC methanol extracts had high collagenase inhibitory activity (88.3%), and the SJ prethanol extracts showed higher elastase inhibitory activity (19.0%) compared to other extracts. Then the tyrosinase inhibitory activity was significantly higher in the SJ and CC methanol extracts (41.8, 30.3%, respectively), whereas prethanol extracts were the lowest. To identify the phenol component contents of SJ and CC extracts, 4-hydroxybenzoic acid, naringenin, naringin and nicotinic acid were measured using LC-MS/MS. As a result, the phenol contents were the highest in SJ methanol extract (4-hydroxybenzoic acid), SJ and CC prethanol extract (naringin and naringenin) and CC prethanol extract (nicotinic acid). Lastly, the antioxidant activity of SJ and CC showed high correlations with polyphenol and flavonoid contents (R = -0.946~0.883). These results suggest that prethanol or methanol extracts of SJ and CC have higher antioxidant activities, anti-aging activity and the potential to be used as material for health functional food and cosmetics.

• Journal of Nutrition and Health
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• v.43 no.6
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• pp.588-596
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• 2010

Most nutrients taken by pregnant women are secreted into their breast milk. Food contains choline together with betaine, and in human body choline is oxidized to betaine which transfer methyl group. The aim of the study was to estimate the concentrations of choline and betaine in breast milk of Korean lactating women and the choline and betaine intakes of their infants. Total choline, free choline and betaine concentrations in breast milk of some lactating women living in Daejon Metropolitan city were analyzed every month by using HPLC-MS and enzymatic method during the first five months. Total choline concentrations of breast milks were 157.64 mg/L (1.52 mmol/L), 157.83 mg/L (1.52 mmol/L), 165.99 mg/L (1.60 mmol/L), 153.67 mg/L (1.48 mmol/L), 145.05 mg/L (1.39 mmol/L) by month after delivery for five months. The concentrations of total choline and free choline in breast milks were not significantly changed for the five months while the betaine concentrations gradually decreased. Daily intake of total choline of the infants appears to be adequate for the infant's requirement according to the US DRI; 124.6 mg/d, 120.9 mg/d, 126.5 mg/d 104.1 mg/d from 2nd to 5th month after birth. Free choline and betaine intakes of the infants were not significantly changed during the four months except showing decrease in betaine intake per kg body weight. Choline intakes of the infants more correlated with choline concentrations of the breast milks (r = 0.982, p = 0.000) than intake amount of the breast milk (r = 0.414, p = 0.028). These results suggest that the choline intake of Korean breast-fed infants appears to be adequate and the intake could be affected by the choline concentration of the breast milk.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.314-334
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• 2013

This study explored an efficient modified Quick, Easy, Cheap, Effective, Rugged and safe (QuEChERS) method combined with liquid chromatography-electrospray ionization with tandem mass spectrometric detection for the analysis of residues of 76 pesticides in brown rice, barley and corn including acidic sulfonylurea herbicides. Formic acid (1%) acid in acetonitrile and dispersive solid phase extractions used for extraction of pesticides and clean-up of the extract respectively. Two fortified spikes at 50 and 200 ng $g^{-1}$ levels were performed for recovery test. Mean recoveries of majority of pesticides at two spike levels ranged from 73.2 to 132.2, 80.9 to 136.8, 66.6 to 143.5 for brown rice, barley and corn respectively with standard error (CV) less than 10%. Good linearity of calibration curves were achieved with $R^2$ > 0.9907 within the observed concentration ranged. The modified method also provided satisfactory results for sulfonylurea herbicides. The method was applied to the determination of residues of target pesticides in real samples. A total of 26 pesticides in 36 out of 98 tasted samples were observed. The highest concentration was observed for tricyclazole at 1.17 mg $kg^{-1}$ in brown rice. This pesticide in two brown rice samples exceeded their MRLs regulated for rice in republic of Korea. Except tricyclazole none of the observed pesticides' concentration was higher than their MRLs. The results reveal that the method is effectively applicable to routine analysis of residues of target pesticides in brown rice, barley and corn.

• Journal of Nutrition and Health
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• v.53 no.3
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• pp.231-243
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• 2020

Purpose: To determine the metabolic influence of the traditional Korean diet (K-diet), which has been regarded as a healthy diet, we investigated the profile of urine organic acids that are intermediates of various types of metabolism including energy metabolism. Methods: Ten women aged 50-60 years were recruited and randomly divided into 2 diet groups, K-diet and control diet, the latter of which is a Westernized Korean diet that is commonly consumed by Koreans nowadays. Before and after the 2-week intervention, 46 urine organic acids were determined using LC/MS/MS, along with clinical parameters. Results: The average concentrations of succinate (4.14 ± 0.84 ㎍/mg creatinine vs. 1.49 ± 0.11, p = 0.0346) and hydroxymethylglutarate (3.67 ± 0.36 ㎍/mg creatinine vs. 2.97 ± 0.29, p = 0.0466), both of which are intermediates of energy metabolism, decreased in the K-diet group after the 2-week intervention, but these were not observed in the control diet group. In particular, the average concentration of succinate in the K-diet group was lower than that in the control group (3.33 ± 0.56 ㎍/mg creatinine vs. 1.49 ± 0.11, p = 0.0284) after 2 weeks. The concentrations of two tryptophan metabolites, 5-hydroxyindolacetate (3.72 ± 0.22 ㎍/mg creatinine vs. 3.14 ± 0.21, p = 0.0183) and indican (76.99 ± 8.35 ㎍/mg creatinine vs. 37.89 ± 10.06, p = 0.0205) also decreased only in the K-diet group. After the 2-week intervention, the concentration of kynurenate, another tryptophan metabolite, was lower in the K-diet group than that in the control diet group (3.96 ± 0.51 ㎍/mg creatinine vs. 2.90 ± 0.22, p = 0.0356). Interestingly, the urine level of kynurenate was positively correlated with BMI (r = 0.61424, p = 0.0003) and total cholesterol (r = 0.46979, p = 0.0088), which decreased only in the K-diet group (239.40 ± 15.14 mg/dL vs. 198.20 ± 13.25, p = 0.0163). Conclusion: The K-diet alters the urinary excretion of organic acids involved in energy metabolism and tryptophan metabolism, suggesting the influence of the K-diet on these types of metabolism. Urine organic acids changed by the K-diet may serve as biomarkers in future studies.

• Korean journal of applied entomology
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• v.10 no.2
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• pp.109-116
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• 1971

The study involved determination of resistance levels of spider mites to Metasystox, Folidol and C-8514 using slide dip technique: a feid trial of alternative acaricides using an O/P resistant strain. 1. Resistant strains of two-spotted spider mite( Tetranychus urticae Koch) were collected from Taegu, Kyungsan, Chunchun, Yesan, Suwon, Osan and Chungju, and kept in the glasshouse through the experiments. Resistant strains of European red mite (Panonychus ulmi Koch) were collected from Chungju, Taegu, Yesan, Chunchun, Suwon and Whasung, and Wonju susceptible was collected. Immediately after collection, tests were applied. All strains except susceptible populations had regularly been sprayed with Organo-phosphates including Parathion (or Folidol), Metasystox, Phenkapton and Trithion for more than the ten Bast years; and Organo-chlorines such as Kelthane and C-8514 more than five years. Comparisons of the resistant strains and susceptible strains at the $LC_50$ values are as follows: a. With Metasystox, resistance levels of the Chungju, Taegu, Yesan, Chunchun and Suwon resistant strains of P. ulmi were respectively, 96,52,4,3 and 2, times as resistant as the Wonju susceptible strain. b. With Folidol, resistance levels of the Chungju, Chunchun, Yesan, Whasung and Suwon strains of P. ulmi showed respectively, 126, 48, 33, 30 and 6-fold resistance levels over the susceptible strain. c. With C-8514, resistance levels of the Taegu, Suwon, Whasung and Chungju strains of P. ulmi were 42, 31, 30 and 20 times greater than the susceptible strain, respectively. d. With Metasystox, resistance levels of the Taegu, Chunchun, Yesan and Suwon, strains of T urticae were respectively, 32,29,25 and 17 times as resistant as the susceptible strain. e. With C-8514, resistance levels of the Taegu, Chungju and Kyungsan strains of T. urticae showed respectively, 59, 29 and 19-fold resistance levels over the Osan strain. 2. Field trials were carried out to assess the toxicities of eleven alternative materials which would be used for control of O/P resistant strain of Panonychus ulmi. The acaricide groups represented were 2 Organo-chlorines (Kelthane MF and Prethylen), 1 carbamate (Furadan), 1 nitrophenyl (Morocide), 1 carbonate (Morestan), 1 tin(Plictran), 1 fluorine (Nissol), 2 mixtures (Fundal and Banmite) and Organo-phosphate (Phenkapton). Of all acaricides tested Kelthane, Plictran, Fundal and Morestan were the most effective; fol]owed by Banmite, Furadan, Prethylen and Nissol. Sumite and Morocide were intermediate, but Phenkapton(Organo-phosphate) was very poor. In other words, a first application of the above outstanding materials gave very high degrees of control of O/P resistant population of European red mite for about 7 to 12 days. However, the results indicate that secondary application would sometimes be necessary. There was no foliage damage to apple trees by any of the acaricides tested of the concentrations used.

• Journal of Food Hygiene and Safety
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• v.31 no.3
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• pp.186-193
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• 2016

An analytical method was developed for the determination of oxathiapiprolin in agricultural commodities. Oxathiapiprolin is a new oomycide (fungicide of piperidinyl thiazole isoxazoline class) which controls downy mildew in cucurbits caused by Pseudoperonospora cubensis (oomycete plant pathogen). Agricultural commodities were extracted with acetonitrile and partitioned with dichloromethane to remove the interference, adjusting pH between 9 and 10 by 1 N sodium hydroxide. After purification by silica SPE cartridge to clean up the interference of organic compounds, they were finally quantified by HPLC-UVD (high performance liquid chromatograph ultraviolet detector) using a wavelength at 260 nm and confirmed by LC-MS (liquid chromatograph mass spectrometer) in electro-spray ionization positive ion mode. The standard calibration curve was linear with coefficients of determination ($r^2$) 1.00 over the calibration ranges (0.025-2.5 mg/L). Recoveries were ranged between 86.7 to 112.7%, with relative standard deviations less than 10% at three concentration levels (LOQ, 10LOQ, and 50LOQ) performing five replicates. The overall results were determined and estimated according to the CODEX guidelines (CAC/GL40). The proposed method for determination of oxathiapiprolin residues in agricultural commodities can be used as an official method.