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http://dx.doi.org/10.14480/JM.2014.12.4.357

Pathogenicity bioassay of entomopathogenic nematode, Steinernema carpocapsae GSN1 strain (Nematoda: Steinernematidae) on the shiitake fungus moth, Morophagoides moriutii (Lepidoptera: Tineidae)  

Kim, Hyeong Hwan (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Dong Hwan (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA)
Cho, Myung Rae (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA)
Yang, Chang Yeol (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA)
Kang, Taek Jun (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA)
Jeon, Sung Wook (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA)
Song, Jin Sun (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, RDA)
Jung, Young Hak (Division of Applied Life Science (BK21+)/Institute of Agriculture and Life Sciences, Gyeongsang National University)
Park, Hae Woong (World Institute of Kimchi, An Annex of Korea Food Research Institute)
Park, Chung Gyoo (Division of Applied Life Science (BK21+)/Institute of Agriculture and Life Sciences, Gyeongsang National University)
Choo, Ho Yul (Division of Applied Life Science (BK21+)/Institute of Agriculture and Life Sciences, Gyeongsang National University)
Publication Information
Journal of Mushroom / v.12, no.4, 2014 , pp. 357-362 More about this Journal
Abstract
Pathogenicity of Steinernema carpocapsae GSN1 strain was evaluated against Morophagoides moriutii larvae. In Petridish tests, insect mortality by the nematode was dose dependent, which increased with dose from 5 to 160 infective juveniles(IJs)/larva. Pathogenicity against fourth-instar larvae was higher than the rate of corresponding second- and third-instar larvae, showing 100% insect mortality with the dose of 40 IJs/$4^{th}$ instar larva and 80 IJs/$2^{nd}$ or $3^{rd}$ instar larvae. Lethal concentration values at 50% ($LC_{50}$) of S. carpocapsae GSN1 strain were 4.2 IJs/$2^{nd}$ instar larva; 8.5 IJs/$3^{rd}$ instar larva; and 2.3 IJs/$4^{th}$ instar larva, respectively. The number of nematodes established in M. moriutii larvae after infection increased in the increment of dose and insect developmental stage. The highest number of nematodes was harvested from fourth instar larvae of M. moriutii at a dose of 160 IJs per larva, showing 22.5 nematodes per insect larva. Nematode reproductive capacity was related to insect developmental stage, showing 6,335 IJs/$2^{nd}$ instar larva, 21,660 IJs/$3^{rd}$ instar larvae, and 88,700 IJs/$4^{th}$ instar larvae.
Keywords
Morophagoides moriutii; mortality; Pathogenicity; Shiitake; Steinernema carpocapsae;
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Times Cited By KSCI : 7  (Citation Analysis)
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