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Generation and Characterization of Monoclonal Antibodies against Human Interferon-lambda1

  • Hong, Seung-Ho;Kim, Jung-Sik;Park, Sun
    • IMMUNE NETWORK
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    • 제8권1호
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    • pp.7-12
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    • 2008
  • Background: Members belonging to the interferon-lambda (IFN-${\lambda}$) family exert protective action against viral infection; however, the mechanisms of their action have remained elusive. To study IFN-${\lambda}$ biology, such as endocytosis of IFN-${\lambda}$, we produced monoclonal antibodies (Abs) against human IFN-${\lambda}$ and examined their usefulness. Methods: We purified recombinant human IFN-${\lambda}$1 expressed in Escherichia coli by using affinity columns. Then, we generated hybridoma cells by fusing myeloma cells with splenocytes from IFN-${\lambda}$1-immunized mice. For evaluating the neutralizing activity of the monoclonal Abs against IFN-${\lambda}$1, we performed RT-PCR for the MxA transcript. In order to study the binding activity of IFN-${\lambda}$ and the monoclonal Ab complex on HepG2 cells, we labeled the monoclonal Ab with rhodamine and determined the fluorescence intensity. Results: Four hybridoma clones secreting Abs specific to IFN-${\lambda}$1 were generated and designated as HL1, HL2, HL3, and HL4. All the Abs reacted with IFN-${\lambda}$1 in the denatured form as well as in the native form. Abs produced by HL1, HL3, and HL4 did not neutralize the induction of the MxA gene by IFN-${\lambda}$1. We also demonstrated the binding of the HL1 monoclonal anbitody and IFN-${\lambda}$ complex on HepG2 cells. Conclusion: Monoclonal Abs against IFN-${\lambda}$1 were produced. These Abs can be used to study the cellular binding and internalization of IFN-${\lambda}$.

이소프로판올-물 混合溶媒中의 리튬, 나트륨 및 칼륨 鹽化沕 溶液의 電導度 (Electric Conductance of Dilute Solutions of Lithium, Sodium and Potassium Chloride in Isopropanol_Water Mixtures)

  • 조병린;이영자;김재빈
    • 대한화학회지
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    • 제20권4호
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    • pp.260-265
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    • 1976
  • 여러 組成의 isopropanol-물 混合溶媒中에서 LiCl, NaCl 및 KCl의 稀薄溶液의 電導導를 測定하였다. 그結果, 濃度에 따르는 當量電鍍度의 변화가 Debye-Huckel-Onsager 식에 대해서 좋은 一致를 보였다. 또한 溶媒中의 isopropanol 組成이 증가함에 따라 極限當量電導度${\Lambda}_0의 값이 급격히 減少하며 이것은 溶媒의 粘度增加 및 誘導常數의 감소로부터 예측되는 바와 같다. 그리고 isopropanol의 몰分率이 0.0, 0.1 및 0.2인 溶媒中에서는 {\Lambda}_0가 {\Lambda}_{0,LiCl} < {\Lambda}_{0,NaCl} < {\Lambda}_{0,KCl}의 順으로 증가하고 있으나, 0.3 몰分率 isopropanol-물中에서는 {\Lambda}_{0,LiCl} < {\Lambda}_{0,KCl} < {\Lambda}_{0,NaCl}의 順으로 바뀌고 있다. 또한 Walden product {\Lambda}_{0{\eta}0}$는 0.1몰分率 isopropanol-물中에서 最大値를 보여 주었다.

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비정질 As-Ge-Se-S 박막에서의 Ag 도핑에 따른 회절효율 특성 연구 (The characteristic of diffraction efficiency depending on Ag doping on film of amorphous AsGeSeS)

  • 이기남;여철호;신경;정홍배
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2004년도 하계학술대회 논문집 Vol.5 No.2
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    • pp.1066-1069
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    • 2004
  • This paper shows the characteristic of diffraction efficiency on film of amorphous AsGeSeS according to Ag doping. We compare amorhous AsGeSeS with the characteristics of AsGeSeS/Ag (10nm) and AsGeSeS/Ag (20m). We made film such as $\lambda$, $\lambda/2$, $\lambda/4$, $\lambda/8on$ the basis of 633nm, which is wavelength of recording laser(He-Ne). The highest diffraction efficiency on AsGeSeS film is $\lambda/4$, followed by $\lambda/2$, $\lambda$, $\lambda/8$. In the case of Ag 10nm, it is followed by $\lambda/4$, $\lambda/2$, $\lambda$, $\lambda/8$. On the occasion of Ag 20nm, it seems that the highest is $\lambda/2$, followed by $\lambda$, $\lambda/4$, $\lambda/8$. In other words, it appears that the highest point on AsGeSeS(158nm) single layer is 0.09%, the one of AsGeSeS(158nm)/Ag(10nm) is 1.6%, and the point of AsGeSeS(316nm)/Ag(20nm) is3.2%. Comparing the highest point in each case, we conclude that there is a distinctive increase in diffraction efficiency according the effect on Ag doping.

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대기압 Ar 직류 글로우 방전에서 인가전압의 파형특성에 따른 광원효율 분석 (The Analysis of Light Emissions on Ar DC Glow Discharge under the Atmosphere Pressure)

  • 소순열
    • 전기전자학회논문지
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    • 제23권3호
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    • pp.865-872
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    • 2019
  • 대기압에서의 Ar 직류 글로우방전 현상을 1차원 유체모델을 통하여 시뮬레이션을 수행하였다. Ar 기체방전의 여기입자들로부터 방출되는 광원으로서 4가지(${\lambda}_{128nm}$, ${\lambda}_{727nm}$, ${\lambda}_{912nm}$ 그리고 ${\lambda}_{966nm}$) 파장의 빛을 검토하였다. 그 결과, 사인형태의 전원 전압을 인가하였을 경우, ${\lambda}_{128nm}$${\lambda}_{727nm}$의 광원이 직류 전압파형을 인가하였을 때보다 거의 2배에 가까운 빛을 방출하는 것을 확인하였다. 또한, 전원전압의 스위칭 시간이 짧을수록, ${\lambda}_{128nm}$${\lambda}_{727nm}$의 방출이 더 많아지는 것을 알 수 있었다. 본 연구에서는 하전입자에 의한 줄 열과 소모 전력에 관해서도 논의하였다.

ONE-PARAMETER GROUPS OF BOEHMIANS

  • Nemzer, Dennis
    • 대한수학회보
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    • 제44권3호
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    • pp.419-428
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    • 2007
  • The space of periodic Boehmians with $\Delta$-convergence is a complete topological algebra which is not locally convex. A family of Boehmians $\{T_\lambda\}$ such that $T_0$ is the identity and $T_{\lambda_1+\lambda_2}=T_\lambda_1*T_\lambda_2$ for all real numbers $\lambda_1$ and $\lambda_2$ is called a one-parameter group of Boehmians. We show that if $\{T_\lambda\}$ is strongly continuous at zero, then $\{T_\lambda\}$ has an exponential representation. We also obtain some results concerning the infinitesimal generator for $\{T_\lambda\}$.

Some Notes on the Fourier Series of an Almost Periodic Weakly Stationary Process

  • You, Hi-Se
    • Journal of the Korean Statistical Society
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    • 제3권1호
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    • pp.13-16
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    • 1974
  • In my former paper [3] I defined an almost periodicity of weakly sationary random processes (a.p.w.s.p.) and presented some basic results of it. In this paper I shall present some notes on the Fourier series of an a.p.w.s.p., resulting from [3]. All the conditions at the introduction of [3] are assumed to hold without repreating them here. The essential facts are as follows : The weakly stationary process $X(t,\omega), t\in(-\infty,\infty), \omega\in\Omega$, defined on a probability space $(\Omega,a,P)$, has a spectral representation $$X(t,\omega)=\int_{-\infty}^{infty}{e^{it\lambda\xi}(d\lambda,\omega)},$$ where $\xi(\lambda)$ is a random measure. Then, the continuous covariance $\rho(\mu) = E(X(t+u) X(t))$ has the form $$\rho(u)=\int_{-\infty}^{infty}{e^{iu\lambda}F(d\lambda)},$$ $E$\mid$\xi(\lambda+0)-\xi(\lambda-0)$\mid$^2 = F(\lambda+0) - F(\lambda-0) \lambda\in(-\infty,\infty)$, assumimg that $\rho(u)$ is a uniformly almost periodic function.

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루푸스 환자의 면역글로불린 λ 경쇄 레파토리 분석 (Analysis of Immunoglobulin λ Light Chain Repertoire in Systemic Lupus Erythematosus)

  • 장지은;이지수
    • IMMUNE NETWORK
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    • 제3권3호
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    • pp.227-234
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    • 2003
  • Background: Immunoglobulin (Ig) light chain repertoire has been implicated as a critical determinant in regulation of autoreactive B cells and production of pathogenic anti-DNA antibodies in systemic lupus erythematosus (SLE). We analyzed the impact of Ig ${\lambda}$ chain repertoire on development of autoimmunity in patients with SLE. Methods: We obtained genomic DNA from individual peripheral CD19+ B cells of 3 untreated active SLE patients, and amplified $V{\lambda}$ rearrangements from each single cell by polymerase chain reaction. Results: A total number of 208 $V{\lambda}J{\lambda}$ rearrangements were analyzed. Analyzed sequences included 158 productive rearrangements and 50 nonproductive rearrangements. The differences in $V{\lambda}$ gene usage in the productive and nonproductive repertoire of SLE patients were found compared to the non-autoimmune individuals. $V{\lambda}$ gene, 9A was significantly overrepresented in nonproducative repertoire of SLE patients (P=0.016). In the productive repertoire, $V{\lambda}$ genes, 3L and 1E were found more often in the SLE patients (P=0.001, P=0.043). When the productive and the nonproductive repertoires were compared, 9A was found significantly less in the productive repertoire in the SLE patients (P=0.000). There were no significant differences in the $J{\lambda}$ gene usage between SLE patients and non-autoimmune individuals, but $J{\lambda}2/3$ gene was the most frequently used in SLE, whereas $J{\lambda}7$ gene was the most frequently used in the normal subjects. In the productive SLE $V{\lambda}$ repertoire, 9.4% of the total sequences employed identical CDR3. It was particularly striking to find 7 identical versions of the 1G-$J{\lambda}2/3$ $V{\lambda}J{\lambda}$ rearrangements from one patient and 3 of the same sequence from another patient. Notably, identical $V{\lambda}$ junctions in the SLE patients utilized significantly more homologous joining compared to $V{\lambda}$ junctions of the normal adults (P=0.044). Conclusion: These data demonstrate regulation of ${\lambda}$ light chain expression in the SLE patients by selection of unique $V{\lambda}$ genes. Also, biased selection and clonal expansion of particular $V{\lambda}$ rearrangements are apparent in the SLE ${\lambda}$ repertoire.

ON A CLASS OF CONSTACYCLIC CODES OF LENGTH 2ps OVER $\frac{\mathbb{F}_{p^m}[u]}{{\langle}u^a{\rangle}}$

  • Dinh, Hai Q.;Nguyen, Bac Trong;Sriboonchitta, Songsak
    • 대한수학회보
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    • 제55권4호
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    • pp.1189-1208
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    • 2018
  • The aim of this paper is to study the class of ${\Lambda}$-constacyclic codes of length $2p^s$ over the finite commutative chain ring ${\mathcal{R}}_a=\frac{{\mathbb{F}_{p^m}}[u]}{{\langle}u^a{\rangle}}={\mathbb{F}}_{p^m}+u{\mathbb{F}}_{p^m}+{\cdots}+u^{a-1}{\mathbb{F}}_{p^m}$, for all units ${\Lambda}$ of ${\mathcal{R}}_a$ that have the form ${\Lambda}={\Lambda}_0+u{\Lambda}_1+{\cdots}+u^{a-1}{\Lambda}_{a-1}$, where ${\Lambda}_0,{\Lambda}_1,{\cdots},{\Lambda}_{a-1}{\in}{\mathbb{F}}_{p^m}$, ${\Lambda}_0{\neq}0$, ${\Lambda}_1{\neq}0$. The algebraic structure of all ${\Lambda}$-constacyclic codes of length $2p^s$ over ${\mathcal{R}}_a$ and their duals are established. As an application, this structure is used to determine the Rosenbloom-Tsfasman (RT) distance and weight distributions of all such codes. Among such constacyclic codes, the unique MDS code with respect to the RT distance is obtained.