• Korean Journal of Plant Resources
• /
• v.22 no.6
• /
• pp.571-583
• /
• 2009

Lam Dong Province of Vietnam has an exceptional diversity of orchids and herbs. At least 920 herb species are widely mentioned in Vietnamese traditional medicinal literature and over 239 Lam Dong wild orchid species are used for attention under the Conservation Vietnamese wild orchids projects. In collaboration with Department of Plant Biotechnology, Dongguk University - South Korea working under supported ODA funds from Korea government (MIFAFF), we will consider how to collecting and preserving these plants in situ or conservating in vitro as a genetic resource.

• Journal of the Korean Society for Precision Engineering
• /
• v.33 no.2
• /
• pp.101-107
• /
• 2016

Laser-Assisted machining (LAM) is a new method for processing hard-to-cut materials. However, curved shapes are difficult to predict the preheating effect of by LAM because heat sources are changed by moving laser module. So, it is necessary to study the preheating effect of the laser heat source irradiated on a 3-dimensionally shaped workpiece, such as a NURBS shaped workpiece. In this study, thermal analysis and preheating experiment of the LAM for the NURBS shaped workpiece are performed. Also, two machining methods are proposed to avoid interference of laser module and cutting tool. The results of the analysis can be applied to various shaped workpieces by LAM.

• Journal of the Korean Society for Precision Engineering
• /
• v.32 no.2
• /
• pp.173-178
• /
• 2015

Laser-assisted machining (LAM) is an effective machining method for processing difficult-to-cut materials. Prediction and estimation of preheating effect of the LAM is difficult because of moving heat source. So it is necessary to study the preheating effect of the laser heat source irradiated on the curved surfaces of workpieces of various shape. In this paper, thermal analysis of the LAM for 3-dimentional workpiece with cylindrical shape was performed. The results of this analysis can be applied to obtain the optimal preheating method and path for LAM of 3-dimensional workpiece.

• Applied Biological Chemistry
• /
• v.36 no.3
• /
• pp.158-162
• /
• 1993

A bilirubin oxidase produced by Penicillium sp. strain LAM 91-89 was purified and partially characterized. The enzyme was purified about 70 folds from culture broth by ethanol precipitation, first and second Sephadex G-200 column chromatography with overall yield of 12%. The molecular weight of the enzyme was estimated to be 53,000 dalton by SDS-PAGE. The optimum pH and temperature was 8.5 and $40^{\circ}C$, respectively. The enzyme was stable in the pH range $6{\sim}10$ and below $40^{\circ}C$. Activity of the enzyme was increased by the addition of $Mg^{2+}$ but was gretly inhibited by $Ag^+,\;Hg^{2+},\;Mn^{2+},\;Pb^{2+}$, iodoacetate, p-chloromercurobenzoic acid and sodium dodecyl sulfate. Among various substrates, bilirubin was favorably reacted and $K_m$ value for bilirubin was $6.67\;{\mu}mole$.

• Horticultural Science & Technology
• /
• v.30 no.3
• /
• pp.338-341
• /
• 2012

Campanula, native to Korea, is potentially an attractive potted or bedding plant. Experiments were conducted to produce a $F_1$ hybrid plant for a reduced plant height, 'Jiknyeo', by a conventional cross breeding between C. punctata Lam. and C. punctata Lam. var. rubriflora Mak. The crossing was carried out in July 2001 and the seeds were harvested in October 2001. Seeds were stored for two months at $5^{\circ}C$ for vernalization, and then were sown in March 2002. The $F_1$ hybrid have a purple flower color, and decreased plant height, leaf length, leaf width, and peduncle length. Each flower maintained freshness for a period of 20 days on the average. The whole flowering period of a plant was four months. The plant has flowering characteristics of an indeterminate inflorescence, prefers low humidity during the whole cultivation period, and requires low temperature in winter for flower bud differentiation.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2013.05a
• /
• pp.1147-1148
• /
• 2013

• Proceedings of the Safety Management and Science Conference
• /
• 2005.11a
• /
• pp.427-449
• /
• 2005

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.36 no.1
• /
• pp.8-13
• /
• 2007

This study was conducted to investigate the effect of mycelia of Lentinus edoes mushroom-cultured Astragalus membranaceus Bunge (LAM) on proliferation of cancer cell lines (Hep3B, MCF-7 and HeLa), sarcoma 180 (S-180), and anti-allergy. In an anti-cancer test using Hep3B (hepatic cancer cell), MCF-7 (breast cancer cell) and HeLa (uterine cancer cell), LAM extract showed higher antiproliferating effect than that of Astragalus membranaceus Bunge (AM) extract. In an anti-cancer testing using Hep3B cells and MCF-7 cells, LAM extract showed growth-inhibitory effect of 65.23% at 3 mg/mL and 69.23% at 5 mg/mL, respectively. In an anti-cancer testing using HeLa cells, LAM extract showed growth-inhibitory effect of 42.01% at 5 mg/mL. In addition, LAM showed the tumor suppressive effect in mice injected with S-180 cells. The growth-inhibitory rates against tumor cells were 47% for LAM and 37% for AM. LAM inhibited histamine release from rat peritoneal mast cells activated by compound 48/80. These results suggest that Lentinus edodes mushroom-cultured herb has an antiproliferating effect against cancer cell lines (Hep3B, MCF-7 and HeLa), S-180 tumor and will be beneficial in the treatment of allergic reaction.

• Journal of Chest Surgery
• /
• v.37 no.12
• /
• pp.1015-1018
• /
• 2004

Lymphangioleiomyomatosis (LAM) is a rare disease in women in childbearing age leading to progressive respiratory failure. LAM is characterized by an abnormal harmartomatous proliferation of smooth muscle cells surrounding the blood vessels, lymphatics and airways in the lung. This proliferation leads to airway obstruction, cystic alveolar change and lymphatic obstruction. Patients present with dyspnea, pneumothorax, cough, chest pain, hemoptysis, and chylous effusion. Although lung transplantation is the only therapeutic modality in end-stage LAM with respiratory failure, there has not been any report of successful treatment in Korea. We report one case of successful left single lung transplantation in a 40 year old woman suffering from end-stage LAM.

• Microbiology and Biotechnology Letters
• /
• v.23 no.5
• /
• pp.506-512
• /
• 1995

A cell aggregation factor produced by Aspergillus sp. LAM 94-142 was purified and partially characterized. The factor was purified about 15 folds from culture broth by IRA 420 and IRC 120 treatment, 1% NaCl added acetone precipitation, and Sepharose 4B column chromatography with overall yield of 48%. It was heteropolysaccharide consisted of mannose, arabinose, and glucose with a molar ratio, 31:17:2, and its molecular weight was estimated to be about 900,000 daltons by Sepharodse 4B gel filtration method. The optimum pH and temperature was 8 and 40$\circ$C, respectively. The factor was stable in pH range of 3-9 and at 100$\circ$C for 90 min. The cell aggregation activity of the factor was inhibited by the addition of Hg$^{2+}$, Fe$^{2+}$, Cu$^{2+}$, and some polypeptides such as milk casein or hemoglobin. The factor aggregated Bacillus subtilis, B. macerans, B. turingiensis, E. coli, Peudomonas aeruginosa, P. fluorescens, P. malophilia, and weakly aggregated Staphylococcus sp., Sarcina lutea, P. putida and Cryptococcus neoformnans, but it didn't aggregate various strains of Candida sp. and Saccharomyces sp.