• Title/Summary/Keyword: L2CS

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Turbulent Properties in the Orion A and ρ Ophiuchus molecular clouds: Observations and preliminary results

  • Yun, Hyeong-Sik;Lee, Jeong-Eun;Choi, Yunhee;Lee, Seokho;Baek, Giseon;Lee, Yong-Hee;Choi, Minho;Kang, Hyunwoo;Tatematsu, Ken'ichi;Offner, Stella S.R.;Gaches, Brandt A.L.;Heyer, Mark H.;Evans, Neal J. II;Yang, Yao-Lun
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.2
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    • pp.79.3-80
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    • 2017
  • Molecular clouds are the sites of stellar birth. Turbulence is a natural phenomenon in molecular clouds, which largely determines the density and velocity fields. Additionally turbulent energy dissipation can affect the gas kinetic temperature via shocks. Turbulence thus controls the mode and tempo of star formation. However, despite its important role in star formation, the properties of turbulence remain poorly understood. As part of the Taeduk Radio Astronomy Observatory (TRAO) Key Science Program (KSP), "Mapping turbulent properties of star-forming molecular clouds down to the sonic scale (PI: Jeong-Eun Lee)", we have been mapping two star-forming clouds, the Orion A and the ${\rho}$ Ophiuchus molecular clouds in 3 sets of lines (13CO 1-0/C18O 1-0, HCN 1-0/HCO+ 1-0, and CS 2-1/N2H+ 1-0) using the TRAO 14-m telescope. We apply a Principal Component Analysis (PCA), which is an useful tool to represent turbulent power spectrum. We will present the preliminary results of our TRAO KSP toward two regions: OMC 1-4 in the Orion A cloud, and L1688 in the ${\rho}$ Ophiuchus cloud.

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Comparison of Two Vitrification Methods of In Vitro Development Oocytes Collected from Porcine Antral Follicles Using Open Pulled Straw (OPS) Techniques

  • An, Mihyun;Hong, Daewuk;Son, Dongsoo;Seok, Hobong
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.84-84
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    • 2002
  • The advantages of the OPS techniques(Vajta G et al, Mol Reprod Dev 51: 53-58,1998) give 1) high survival rates of various types of eggs, 2) quick and simple process, 3) inexpensive equipment and reduced chilling injury. The efficiency of IVM/IVF technique in the porcine species is relatively lower than that obtained in other species such as ruminants. Two experiments were designed to investigate the effects of in-vitro fertilization of porcine oocytes matures using different OPS protocol for chilling and warming of vitrification. Porcine oocytes from ovaries collected at abattoir were matured for 44 hours in TCM199 Earle's salt supplemental with pyruvate, pff, L-cysteine, hormones and gentamycin. Oocytes were denuded and fertilized with frozen boar semen by common method. Porcine embryos produced routinely by in-vitro culture system of NCSU23 medium. The vitrification and the warming were conducted by OPS method with the glass micropipette instead of straw vessels and modified the protocol of G.Vajta(1999). In Exp 1, Chilling/Warming:Holding Medium(HM)+EG+DMSO/HM +sucrose Medium(SM) at 39$^{\circ}C$ warm stage. In Exp 2, : PBS+CS+EG+Ficoll+ Trehalose/PBS+Trehalose at 25$^{\circ}C$ stage. Filling, freezing, packing, thawing out and further culturing were performed to follow the basic protocol of G Vajta. During IVM-lVC and post-warming, fertilization parameter and developmental potential were compared to and statistically analysed. It was not significantly different from Exp 1 and Exp 2 but 25$^{\circ}C$ of stage was slightly higher on the morula/blastocyst forming rate and better atmosphere for worker than that at 39$^{\circ}C$ stage.

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Anti-oxidant, Anti-inflammatory, and Wound Healing Activities of Selaginella tamariscina Leaf Extract (부처손 잎 추출물의 항산화, 항염 및 피부재생 효능)

  • Kim, Yu-Jin;Lee, Ji-An
    • Journal of Convergence for Information Technology
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    • v.11 no.4
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    • pp.194-202
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    • 2021
  • The leaves of Selaginella tamariscina were used for the treatment of many diseases in traditional medicine. In the study, antioxidant, anti-inflammatory, and wound healing activities of the hot-water extract(STW) and 80%ethanol extract(STE) obtained from S. tamariscina were evaluated. As a result, the polyphenol content of STW and STE were 38.108±0.766 mg/g and 17.927±1.064 mg/g, respectively. The DPPH and ABTS radical scavenging activities with the IC50 values of the STW were over 2 times lower than that of the STE. In the MTT assay, RAW264.7 cell viability of two extracts was decreased by about 6% at 1 mg/mL, whereas for HaCaT cell viability increased by 18% at 50 ㎍/mL. In addition, STW and STE suppressed the production of nitric oxide(NO), Tumor-necrosis(TNF)-��. COX-2 and PGE2 in lipopolysaccharide(LPS) induced RAW264.7 cells. Furthermore, the STE showed wound healing effect through the promotion of skin cell migration in TNF-�� stimulated human keratinocytes. These results indicated that the STW and STE have the potential to be used as a new cosmetic active ingredients in skin care.

Expression of gus and gfp Genes in Ggrlic (Allium sativum L.) Cells Following Particle Bombardment Transformation

  • Lacorte, Cristiano;Barros, Daniella
    • Journal of Plant Biotechnology
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    • v.2 no.3
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    • pp.135-142
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    • 2000
  • The activity of promoter sequences was evaluated in garlic cells using the $\beta$-glucuronidase (GUS) gene as a reporter. Histochemical GUS assay indicated transient GUS activity in leaf, callus and root cells 48 hours after particle bombardment transformation. Quantitative fluorometric assays in extracts of transformed leaves demonstrated that the CsVMV promoter induced the highest level of gene expression, which was, on average, ten fold the level induced by CaMV35S and by the Arabidopsis Act2 promoters and two fold the level expression observed with a construct containing a double CaMV35S plus the untranslated leader sequence from AMV. No activity or very low levels were observed when cells were transformed with plasmids rontaining the typical monocot promoters, Actl, from rice or the Ubi-1, from maize. The green fluorescent protein (GFP) was also tested as a marker gene for garlic transformation. Intense fluorescence was observed in leaf, callus and root cells transformed with a construct containing the gfp gene under control of the CaMV35 Promoter. No fluorescence was detected when the gfp was under control of the Ubi-1 promoter.

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Antioxidant and Anti-inflammatory activity of Silvetia siliquosa extract (Silvetia siliquosa 추출물의 항산화 및 항염효과)

  • Kim, Kyoung-Sook;Kim, Sook-hee
    • Journal of Convergence for Information Technology
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    • v.11 no.8
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    • pp.232-239
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    • 2021
  • In this study, the antioxidant and anti-inflammatory properties of Silvetia siliquosa extracts were identified. Antioxidant experiments included polyphenol concentration measurements, flavonoid concentration measurements, DPPH experiments, ABTS experiment NO experiments, and FRAP experiments. For polyphenols, 54.85 ± 2.79 mg/g was shown. Flavonoids showed 18.70 ± 5.26 mg/g. The DPPH experiment showed an antioxidant function of 3.950 mg ascorbic acid/g extract, the ABTS experiment showed an antioxidant function of 7.418 mg ascorbic acid/g extract, and the NO experiment showed an antioxidant function of 6.056 mg ascorbic acid/g extract. In FRAP, 1 mg of the moxibustion extract showed a reduction of 3.633 ± 0.280 ㎍ of ascorbic acid. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions against inflammation induced by LPS. In cytotoxicity experiments, Silvetia siliquosa extracts showed a cell survival rate of more than 80% at all concentrations, and an inflammatory inhibition of 26.94±0.52% at a concentration of 100 ㎍/mL. These results indicate that Silvetia siliquosa extract is available as an anti-inflammatory cosmetic material.

Antioxidant and Anti-inflammatory activity of Sargassum patens extract (쌍발이모자반(Sargassum patens) 추출물의 항산화 및 항염효과)

  • Kim, Sook-hee
    • Journal of Convergence for Information Technology
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    • v.11 no.7
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    • pp.264-271
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    • 2021
  • In this study, the antioxidant and anti-inflammatory properties of Sargassum patens extracts were identified. Antioxidant experiments included polyphenol concentration measurements, flavonoid concentration measurements, DPPH experiments, ABTS experiment NO experiments, and FRAP experiments. For polyphenols, 18.99±0.69 mg/g was shown. Flavonoids showed 11.89±1.16 mg/g. The DPPH experiment showed an antioxidant function of 19.78 mg ascorbic acid/g extract, the ABTS experiment showed an antioxidant function of 63.64 mg ascorbic acid/g extract, and the NO experiment showed an antioxidant function of 7.966 mg ascorbic acid/g extract. In FRAP, 1 mg of the moxibustion extract showed a reduction of 2.089 ㎍ of ascorbic acid. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions against inflammation induced by LPS. In cytotoxicity experiments, Sargassum patens extracts showed a cell survival rate of more than 80% at all concentrations, and an inflammatory inhibition of 30.64±0.23% at a concentration of 100 ㎍/mL. These results indicate that Sargassum patens extract is available as an anti-inflammatory cosmetic material.

The Effects of Misutkaru Water Drink on Relieving Constipation and Physiological Measurements: A Preliminary Study for the Development of Convergent Misutkaru Water Drink Intake Program in Patients with Hemodialysis (혈액 투석 환자의 변비 완화 및 생리적 지표 향상을 위한 융합형 미숫가루 음용 섭취 프로그램 개발 예비 조사)

  • Shin, Na-yeon;Park, Hyo-sun
    • Journal of Convergence for Information Technology
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    • v.9 no.5
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    • pp.40-47
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    • 2019
  • The purpose of this study aimed to develop the misutkaru water drink intake program that was possible to compare the effects of misutkaru drink on relieving constipation and physiological measurementson relieving constipation of the patients with hemodialysis. This study was non-equivalent control group pretest-posttest design with 42 patients in a general hospital in S city, Korea. During the experiment, the participants had to drink misutkaru water in the morning and past the 7 days after intake as constipation assessment scale, potassium and phosphorus were measured. The data were examine using t-test, Fisher's exact test, paired t-test. After misutkaru drinking intervention, constipation (z=-2.66, p=.002) decreased significantly improvement. This suggests that misutkaru water drinking intervention improved constipation in patients with hemodialysis.

Evaluation of Clinical Usefulness of Gamma Glutamyl Transferase as a Surrogate Marker for Metabolic Syndrome in Non Obese Adult Men (비만하지 않은 성인 남성에서 대사증후군의 대리 표지자로서 감마 글루타밀 전이효소의 임상적 유용성 평가)

  • Shin, Kyung-A;Kim, Eun Jae
    • Journal of Convergence for Information Technology
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    • v.10 no.12
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    • pp.146-155
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    • 2020
  • This study was to evaluate the usefulness of gamma glutamyl transferase (GGT) as a surrogate marker predicting metabolic syndrome. 7,155 non obese men over the age of 20 were studied as subjects. The criteria for diagnosing MetS were the National Cholesterol Education Program - Third Adult Treatment Panel (NCEP-ATP III). The risk of developing MetS according to GGT was conducted logistic regression analysis, and the ROC (receiver operating characteristic) curve was obtained to confirm GGT ability to predict the risk of MetS. Regardless of age and body mass index, MetS had a 7.09 times higher risk of onset in the fourth quartile than in the first quartile of GGT (p<0.001). The AUC (area under the curve) of GGT for the diagnosis of MetS was 0.715, and the cutoff value of GGT was 40.0 U/L, the sensitivity was 65.0%, and the specificity was 70.2%. Therefore, GGT is considered to be a useful diagnostic index for diagnosing MetS.

Anti-inflammatory and Antioxidant Effects of Hot Water Extracts from Kaempferia Galanga L (삼내자 열수추출물의 항산화 및 항염 효과)

  • Chan, Ching Yuen Venus;Lee, Ji-An
    • Journal of Convergence for Information Technology
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    • v.9 no.6
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    • pp.218-226
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    • 2019
  • In this study, we investigated the possibility of Kaempferia Galanga(KG) hot water extract on the antioxidant, cytotoxic and anti-inflammatory efficacy as a cosmetic ingredient. Antioxidant effects were evaluated based on DPPH and ABTS radical scavenging activity, FRAP assay, and total polyphenol contents. The MTT assay was used to confirm the cell toxicity in mouse macrophage RAW264.7 cells. Anti-inflammatory effects were also investigated in LPS-induced RAW264.7 cells by measuring secretion of NO, $TNF-{\alpha}$ and iNOS, $TNF-{\alpha}$ mRNA expression level. As a result, DPPH and ABTS radical scavenging activities were increased in a concentration-dependent manner. The ferric reducing antioxidant power(FRAP) was the highest at 5 mg/mL as 24.5 uM. The measurements of total polyphenol content was $1.28{\pm}0.064mg\;GAE/g$. The cytotoxicity of the KG extract results showed no cytotoxicity at concentration of 0.625 to 2.5 mg/mL. In addition, the extract of KG significantly suppressed the LPS-induced nitrite, $TNF-{\alpha}$ secretion and the mRNA expression of iNOS, $TNF-{\alpha}$ in RAW264.7 cells. Taken together, these data suggest that the KG hot water extracts can be used as a safe and functional cosmetic raw material.

Quality Characteristics of Sulgidduk Added with Cabbage Powder (양배추 분말을 첨가한 설기떡의 품질 특성)

  • Yang, Mi-Ok
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.5
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    • pp.729-735
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    • 2009
  • The objective of this study was to evaluate the quality characteristics of Sulgidduk added with cabbage powder. The moisture contents of Sulgidduk added with cabbage powder ranged from 37.2 to 33.7% and were significantly lower than those of the control. As the amount of cabbage powder increased, the L-value was reduced, while the a- and b-values were increased. In terms of textural characteristics, Hardness, Adhesiveness, Springiness, Cohesiveness, Gumminess, and Chewiness decreased with greater concentrations of cabbage powder. Sensory evaluation revealed that the favorite degree of color, flavor and taste received high increasing scores with increasing addition amounts of cabbage powder, while the Chewiness did not differ significantly (p<0.05) among the samples. The moistness found to be highest in the control, while it decreased as the amount of cabbage powder increased. The overall acceptance was highest as (6.00) for the Sulgidduk (CS6) that contained 6% of the cabbage powder. Overall, the results of this study indicate that adding 6% cabbage powder to Sulgidduk was produced the optimal results, and provided good quality characteristics and high overall acceptance.

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