• 한국구조물진단유지관리공학회 논문집
• /
• 제6권2호
• /
• pp.121-126
• /
• 2002

Due to few earthquakes in our country, one generally has thought to be safe from earthquakes. However, severe earthquakes occurred in Dangsan and Hyogohyeon which one had regarded as the zone that had not been risky for earthquakes, so that so many people died and a lot of buildings and bridges were destroyed. This event surprised our country and we undertook preparation for earthquakes on the full scale. The concept of seismic design was induced in the country which was poor in it for the scarcity of recognition and insufficiency of funds. Recently, many specialists are enforcing the provisions of seismic design. Therefore, this study introduces the method which combines PC-LEADeR( design program for L.R.B.) with LUSAS(linear elastic analysis) and performs the seismic isolation design more elaborately and simply. It verifies the propriety of that method, and it also examine the factors that affect the response of the bridges. Seismic isolation design for bridge using L.R.B. provides both economical efficiency and superior seismic performance. Second, the results between by the method proposed and by time history analysis have 20% error at the maximum. That is, the method proposed very appropriate.

• 대한환경공학회지
• /
• 제36권11호
• /
• pp.764-770
• /
• 2014

본 연구는 electron impact-gas chromatography/mass spectrometer (EI-GC/MS)를 이용하여 N-nitrosamines 분석하는 방법의 분리성, 정성 및 정량적 유효성을 평가하였다. 극미량의 검출을 위해 시료전처리는 자동고상추출과 질소농축과정을 거쳐 수행하였다. 표준시료를 전처리 없이 EI-GC/MS (SIM)와 EI-GC/MS/MS (MRM)를 이용하여 직접 분석한 결과, 두 방법 모두 유사한 감응도를 보였다. 반면, 전처리한 표준시료를 EI-GC/MS로 분석한 경우 불순물 피크에 의한 간섭영향에 의해 낮은 ng/L 수준의 정량은 어려운 것으로 나타났다. 8종의 N-nitrosamines에 대한 EI-GC/MS/MS 분석결과, 방법검출한계 및 정량한계는 각각 0.76~2.09 ng/L, 2.41~6.65 ng/L 수준으로 기존 분석방법에 비하여 낮게 나타났다. 첨가농도 10, 20, 100 ng/L에 대한 실험에서 정밀도(1.2~13.6%)와 정확도(80.4~121.8%) 모두 만족하였으며, 검량선의 직선성에 대한 결정계수($R^2$)도 0.999 이상이었다. 환경시료에 대한 대체표준물질(NDPA-$d_{14}$)의 회수율도 86.2~122.3%을 나타내어, 본 연구에서 평가된 방법으로 N-nitrosamines의 정밀분석이 가능함을 검증하였다.

• 대한환경공학회지
• /
• 제37권5호
• /
• pp.262-268
• /
• 2015

본 연구는 on-line micro extraction by packed sorbent (on-line MEPS)와 시료다량주입장치인 programmed temperature vaporizer (PTV) injector를 결합한 가스크로마토그래피 질량분석 시스템을 이용하여 유기인계 농약인 methyldemetone-S, diazinon, fenitrothion, parathion, phentoate, O-ethyl O-(4-nitrophenyl) phenylphosphonothioate (EPN)과 카바이트계인 carbaryl에 대한 동시 수질분석법을 확립하였다. MEPS 내 sorbent는 polystyrene divinylbenzene (PDVB) 재질을 이용하였다. 시료전처리시 추출용매 종류, pH, 추출용매량 및 시료주입왕복횟수가 분석에 미치는 영향과 정도보증(QA/QC), 그리고 환경시료에 대한 각 물질의 회수율을 평가하였다. 추출용매는 acetone과 dichloromethane을 80 : 20 (v/v)으로 혼합하여 사용하였고, 내부표준물질과 황산을 첨가한 시료 1 mL를 대상으로 추출용매량 $30{\mu}L$, 시료주입왕복횟수를 7회로 추출하여 분석조건을 최적화하였다. pH가 낮을수록 Diazinon의 분석감응도는 감소한 반면 carbaryl의 분석감응도는 증가하였다. 정도보증결과 항목별 방법검출한계 및 정량한계는 각각 $0.02{\sim}0.18{\mu}g/L$, $0.08{\sim}0.59{\mu}g/L$로 낮았으며, 농도범위 $0.5{\sim}5.0{\mu}g/L$ 수준에서 정밀도와 정확도 범위는 각각 1.5~11.5%, 83.3~129.8%로 나타났다. 환경시료 중 carbaryl을 제외한 모든 항목의 회수율은 75.7~129.3%로 적합하였다. Carbaryl에 대한 회수율은 수돗물, 지하수, 하천수 분석에서 적합한 범위를 나타냈으나, 하수처리방류수에서는 200% 이상으로 만족하지 못하였다.

• Toxicological Research
• /
• 제29권4호
• /
• pp.293-298
• /
• 2013

A method of detecting lead was developed using square wave anodic stripping voltammetry (SWASV) with DNA-carbon nanotube paste electrode (CNTPE). The results indicated a sensitive oxidation peak current of lead on the DNA-CNTPE. The curves were obtained within a concentration range of 50 $ngL^{-1}-20mgL^{-1}$ with preconcentration time of 100, 200, and 400 sec at the concentration of $mgL^{-1}$, ${\mu}gL^{-1}$, and $ngL^{-1}$, respectively. The observed relative standard deviation was 0.101% (n = 12) in the lead concentration of 30.0 ${\mu}gL^{-1}$ under optimum conditions. The low detection limit (S/N) was pegged at 8 $ngL^{-1}$ ($2.6{\times}10^{-8}M$). Results showed that the developed method can be used in real-time assay in vivo without requiring any pretreatment and pharmaceutical samples, and food samples, as well as other materials requiring water source contamination analyses.

• 전기전자학회논문지
• /
• 제20권2호
• /
• pp.152-162
• /
• 2016

전기 저항률 단층촬영법(ERT)은 표면 전극으로부터 측정된 전압을 사용하여 물체 내부의 임피던스 분포를 영상화하는 기술이다. ERT 역문제는 비정치성(ill-posedness)이 매우 심하여 영상복원의 수렴성을 확보하기 위해 조정방법이 사용된다. 사용된 조정방법에 따라 영상복원 성능이 달라지므로 상황에 따라 보다 강건한 영상 복원 성능을 얻기 위해, 서로 다른 영상복원 특성을 나타내는 L1-norm 조정방법과 Total Variation (TV) 조정방법의 두 개의 모드가 상호작용하는 상호작용 이중-모드 조정방법을 제안하였다. 제안한 이중-모드 조정방법은 실제 상황에 따라 달라지는 모드 확률을 계산하고 이에 근거하여 적합한 모드를 선택하거나 두 개의 모드를 결합한다. 모의실험을 수행하여 제안된 기법의 영상 복원 성능을 평가한 결과 비교적 양호한 성능을 나타내었다.

• 한국지반환경공학회 논문집
• /
• 제20권9호
• /
• pp.13-19
• /
• 2019

Dynamic earth pressure is considered an important parameter in the design of embedded structures. In current engineering design simplified methods developed either for yielding or non-yielding structures are utilized to predict resultant dynamic pressure. The applicability of these equations to embedded structures have not yet been reported. In this study we perform a suite of equivalent linear time history analysis for a range of embedded structure configurations. Numerically calculated dynamic pressure is shown to depend on the flexibility ratio (F), aspect ratio (L/H) of the embedded structure, and ground motion. Increase in L/H and intensity increases the magnitude of dynamic pressure. An increase in F decreases the dynamic pressure. Overall, the trends highlight the need for development of new method that accounts for F and L/H to calculate the dynamic pressure for the performance-based design of embedded structures.

• 대한화학회지
• /
• 제44권2호
• /
• pp.95-101
• /
• 2000

2-hydroxy-3-hydroxymethy1-5-methylbenzaldehyde(HHMMB)와 ethylenediamine 혹은 1,3-dia-minopropane의 축합반응에 의해 6-배위 칸막이 리간드, $H_4L[A]$와 $H_4L[B]$를 각각 얻은 후에 거대비고리 리간드 착물, $Cu(H_2L[A]).H_2O$, $Cu(H_2L[B]).H_2O$, CuFe(L[A]($NO_3$).$4H_2O$, CuFe(L[B])($NO_3$).$4H_2O$, [$CuGd(H_2L[A])(NO_3)_2](NO_3).2CH_3OH$, [CuGd($H_2L$[B])($NO_3)_2$]($NO_3).2CH_3OH를 합성하였다. 2,6-diformyl-p-clesol (DFPC)로부터 합성한 Ln-거대고리([20]DOTA)착물,[Ln([20]DOTA)($NO_3)(H_2O)$]($NO_3$)2.$xH_2O${Ln(III)=Pr, Sm, Gd, Dy를 methanol 용매에 2일 정도 정치함으로써 [Ln([20]DOTA)($NO3$)($CH_3OH)]^{2+}$을 얻을 수 있다. 이 lanthanide-[20]DOTA착물에서 배위된 $CH_3OH$이 주게원자가 N 혹은 O인 두자리 보조리간드, $L_a$(=o-phenylenediamine,1,10-phenanthroline, ethylenediamine,oxalic acid, malonic acid, acethyl-acetone에 의해 치환될 때 평형상수(K)를 $25^{\circ}C\;{\mu}=0.1M\;NaClO_4$ 에서 분광학적 방법으로 결정하였다. 보조리간드의 $pK_a$는 o-Phenylenediamine < 1,10-ptlenanthroline < ehylenediamine,oxalic acid < malonic acid < acethylacetone이며, 이때 평형상수, K는 ethylenediamine < 1,10-phenanthroline < o-phenylenediamine,acethylacetone < malonic acid < oxalic acid 경향으로 변하였다.

• Bulletin of the Korean Chemical Society
• /
• 제30권1호
• /
• pp.61-66
• /
• 2009

An analytical method based on solid-phase extraction and gas chromatography / mass spectrometry has been developed for measurement of acaricides (amitraz, bromopropylate, coumaphos, cymiazole, and tetradifon) in honey sample. In the stability test of acaricides in honey, amitraz underwent a rapid degradation into 2,4-dimethylaniline (DMA), 2,4-dimethylphenylformamide (DMPF), and N-(2,4-dimethylphenyl)-N'-methylformamidine (DMPMF), whileas other acaricides were found to be stable even for over three months. Extraction of five acaricides from 5g of honey sample was carried out by liquid-liquid extraction using 20mL of ethylacetate. For purification, Florisil-SPE cartridge with elution of 5mL of n-hexane/ acetone (55:45, v/v) was found to remove interferences effectively. Quantification was performed using gas chromatography / mass spectrometry in the selected ion monitoring mode. Spiking experiments were carried out to determine the recovery, precision, and limits of detection (LODs) of the method. The overall recovery values from honey spiked at 0.02 and 0.20 ${\mu}g/g$ levels, respectively, were found to be greater than 75% for all acaricides. The method detection limits for acaricides were ranged from 0.1 to 3 ppb. The developed method in this study was applied for the monitoring of acaricides in honey products collected from urban markets in Korea.

• Structural Engineering and Mechanics
• /
• 제20권4호
• /
• pp.421-434
• /
• 2005

In this paper, the finite element method is applied to investigate the effect of the lateral boundary in homogenous soil on the seismic response of a superstructure. Some influencing factors are presented and discussed, and several parameters are identified to be important for conducting soil-structure interaction experiments on shaking tables. Numerical results show that the cross-section width L, thickness H, wave propagation velocity and lateral boundaries of soil layer have certain influences on the computational accuracy. The dimensionless parameter L/H is the most significant one among the influencing factors. In other words, a greater depth of soil layer near the foundation should be considered in shaking table tests as the thickness of the soil layer increases, which can be regarded as a linear relationship approximately. It is also found that the wave propagation velocity in soil layer affects the numerical accuracy and it is suggested to consider a greater depth of the soil layer as the wave propagation velocity increases. A numerical study on a soil-structure experimental model with a rubber ring surrounding the soil on a shaking table is also conducted. It is found the rubber ring has great effect on the soil-structure interaction experiments on shaking table. The experimental precision can be improved by reasonably choosing the elastic parameter and width of the rubber ring.

• 대한수의학회지
• /
• 제33권3호
• /
• pp.393-406
• /
• 1993

Antibacterial substances produced by Lactobacillus casei subsp. and Streptococcus faecium were examined for its antibacterial effects against some pathogenic bacteria. They were partially purified with ammonium sulfate precipitation, methanol-acetone extraction, G-50 gel filtration and examined its characteristics. When L casei subsp. and Str faecium were cultivated in MRS broth, stationary phase of L casei is until 24 hours and Str faecium is 20 hours. pH change of the cultured medium was both decreased after 12 hours and then constant at pH 4.5~4.6 after 28 hours. MRS broth culture fluids of L casei subsp. and Str faecium appeared the antibacterial effects by the spot-on-the-lawn method against ETEC, Sal pullorum and Sta aureus. Culture filtrates of L casei subsp. and Str faecium also appeared the antibacterial effects by the disc diffusion method. Culture filtrates of L casei sub. rhamnosus 7469 produced 0.032M of lactic acid and 0.01M of acetic acid. Str faecium 27273 also produced 0.027M of lactic acid and 0.01M of acetic acid. Protein concentrations of culture filtrates produced by L casei sub rhamnosus 7469 and Str faecium 27273 was $495{\mu}g/m{\ell}$ and $594{\mu}g/m{\ell}$, respectively. Antibacterial substances which are partially purified by ammonum sulfate precipitation, methanol-acetone extraction and G-50 gel filtration inhibit the growth of ETEC, Sal pullorum and Sta aureus. Characteristics of purified antibacterial substances was examined. Its molecular weight was about 31Kd, stabilized at $100^{\circ}C/20min.$ and some of proteolytic enzyme treatment.