Browse > Article
http://dx.doi.org/10.5487/TR.2013.29.4.293

Real-time Assay of Toxic Lead in In Vivo Living Plant Tissue  

Ly, SuwYoung (Biosensor Research Institute in Seoul National University of Science and Technology)
Kim, Nack Joo (Dept. of Fine Chemistry, Seoul National Univ. of Science and Technology)
Youn, Minsang (Advanced Scientific Research Group in Shinil High School)
Kim, Yongwook (Advanced Scientific Research Group in Shinil High School)
Sung, Yeolmin (Advanced Scientific Research Group in Shinil High School)
Kim, Dohoon (Advanced Scientific Research Group in Shinil High School)
Chung, Tackhyun (Advanced Scientific Research Group in Shinil High School)
Publication Information
Toxicological Research / v.29, no.4, 2013 , pp. 293-298 More about this Journal
Abstract
A method of detecting lead was developed using square wave anodic stripping voltammetry (SWASV) with DNA-carbon nanotube paste electrode (CNTPE). The results indicated a sensitive oxidation peak current of lead on the DNA-CNTPE. The curves were obtained within a concentration range of 50 $ngL^{-1}-20mgL^{-1}$ with preconcentration time of 100, 200, and 400 sec at the concentration of $mgL^{-1}$, ${\mu}gL^{-1}$, and $ngL^{-1}$, respectively. The observed relative standard deviation was 0.101% (n = 12) in the lead concentration of 30.0 ${\mu}gL^{-1}$ under optimum conditions. The low detection limit (S/N) was pegged at 8 $ngL^{-1}$ ($2.6{\times}10^{-8}M$). Results showed that the developed method can be used in real-time assay in vivo without requiring any pretreatment and pharmaceutical samples, and food samples, as well as other materials requiring water source contamination analyses.
Keywords
DNA-CNTPE; Lead anodic stripping voltammetry; Low concentration; In vivo; Plant; Tap water;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Dorman, R.V. and Freeman, E.J. (2002) Lead dependent effects on arachidonic acid accumulation and the proliferation of vascular smooth muscle. J. Biochem. Mol. Toxicol., 16, 245-253.   DOI   ScienceOn
2 Karakaya, A.E., Ozcagli, E., Ertas, N. and Sardas, S. (2005) Assessment of abnormal DNA repair responses and geno-toxic effects in lead exposed workers. Am. J. Ind. Med., 47, 358-363.   DOI   ScienceOn
3 Cervantes, M.C., David, J.T., Loyd, D.R., Salinas, J.A. and Delville, Y. (2005) Lead exposure alters the development of agonistic behavior in golden hamsters. Dev. Psychobiol., 47, 158-165.   DOI   ScienceOn
4 Sanna, E., Liguori, A., Palmas, L., Soro, M.R. and Floris, G. (2003) Blood and hair lead levels in boys and girls living in two Sardinian towns at different risks of lead pollution. Ecotoxicol. Environ. Saf., 55, 293-299.   DOI   ScienceOn
5 Yokel, J. and Delistraty, D.A. (2003) Arsenic, lead, and other trace elements in soils contaminated with pesticide residues at the hanford site (USA). Environ. Toxicol., 18, 104-114.   DOI   ScienceOn
6 Slobozhanina, E.I., Kozlova, N.M., Lukyanenko, L.M., Oleksiuk, O.B., Gabbianelli, R., Fedeli, D., Caulini, G.C. and Falcioni, G. (2005) Lead-induced changes in human erythrocytes and lymphocytes. J. Appl. Toxicol., 25, 109-114.   DOI   ScienceOn
7 Kozelka, P.B., Sanudo-Wilhelmy, S., Flegal, A.R. and Bruland, K.W. (1997) Physico-chemical speciation of lead in south San Francisco bay. Estuarine Coastal Shelf Sci., 44, 649-658.   DOI   ScienceOn
8 Scarponi, G., Barbante, C., Turetta, C., Gambaro, A. and Cescon, P. (1997) Chemical contamination of antarctic snow: The case of lead. Microchem. J., 55, 24-32.   DOI   ScienceOn
9 Liu, W. and Lee, H.K. (1999) Simultaneous analysis of lead, mercury and selenium species by capillary electrophoresis with combined ethylenediaminetetraacetic acid complexation and field-amplified stacking injection. Electrophoresis, 20, 2475-2483.   DOI
10 Yang, J.L., Wang, L.C., Chang, C.Y. and Liu, T.Y. (1999) Singlet oxygen is the major species participating in the induction of DNA strand breakage and 8-hydroxydeoxyguanosine adduct by lead acetate. Environ. Mol. Mutagen., 33, 194-201.   DOI
11 Chen, S.M. and Chen, S.V. (2003) The interaction of watersoluble iron porphyrins with DNA films and the electrocatalytic properties for inorganic and organic nitro compounds. Electrochim. Acta, 48, 4049-4060.   DOI   ScienceOn
12 De Donato, A. and Gutz, I.G.R. (2005) Fast mapping of gunshot residues by batch injection analysis with anodic stripping voltammetry of lead at the hanging mercury drop electrode. Electroanalysis, 17, 105-112.   DOI   ScienceOn
13 Tsai, Y.C., Davis, J., Compton, R.G., Ito, S. and Ono, N. (2001) Polypyrrole coated mercury film electrodes for sono-ASV analysis of cadmium and lead. Electroanalysis, 13, 7-12.   DOI
14 Casado, M., Daunert, S. and Valiente, M. (2001) Lead-selective electrode based on a quinaldic acid derivative. Electroanalysis. 13, 54-60.   DOI   ScienceOn
15 Angelone, L.M., Potthast, A., Segonne, F., Iwaki, S., Belliveau, J.W. and Bonmassar, G. (2004) Metallic electrodes and leads in simultaneous EEG-MRI: specific absorption rate (SAR) simulation studies. Bioelectromagnetics, 25, 285-295.   DOI   ScienceOn
16 Degefa, T.H., Chandravanshi, B.S. and Alemu, H. (1999) Differential pulse anodic stripping voltammetric determination of lead(II) with N-p-chlorophenylcinnamo-hydroxamic acid modified carbon paste electrode. Electroanalysis, 11, 1305-1311.   DOI
17 Honeychurch, K.C., Hart, J.P. and Cowell, D.C. (2000) Voltammetric behavior and trace determination of lead at a mercury-free screen-printed carbon electrode. Electroanalysis, 12, 171-177.   DOI
18 Wang, J., Lu, J., Hocevar, S.B. and Ogorevc, B. (2001) Bismuth-coated screen-printed electrodes for stripping voltammetric measurements of trace lead. Electroanalysis, 13, 13-16.   DOI
19 da Silva, S.M. (1998) Determination of lead in the absence of supporting electrolyte using carbon fiber ultramicroelectrode without mercury film. Electroanalysis, 10, 722-725.   DOI   ScienceOn
20 Wang, J., Liu, G. and Merkoi, A. (2003) Particle-based detection of DNA hybridization using electrochemical stripping measurements of an iron tracer. Anal. Chim. Acta, 482, 149-155.   DOI   ScienceOn
21 de los Santos Alvarez, P., de los Santos Alvarez, N., Lobo Castanon, M.L., Miranda Ordieres, A.J. and Tunon Blanco, P. (2006) Amplified label-free electrocatalytic detection of DNA in the presence of calcium ions. Biosens. Bioelectron., 21, 1507-1512.   DOI   ScienceOn
22 Gil Ede, S., Serrano, S.H., Ferreira, E.I. and Kubota, L.T. (2002) Electrochemical evaluation of rhodium dimer-DNA interactions. J. Pharm. Biomed. Anal., 29, 579-584.   DOI   ScienceOn
23 Wang, J. and Musameh, M. (2004) Electrochemical detection of trace insulin at carbon-nanotube-modified electrodes. Anal. Chim. Acta, 511, 33-36.   DOI   ScienceOn
24 Ariza, M.E. and Williams, M.V. (1999) Lead and mercury mutagenesis: Type of mutation dependent upon metal concentration. J. Biochem. Mol. Toxicol., 13, 107-112.   DOI
25 Roscoe, R.J., Gittleman, J.L., Deddens, J.A., Petersen, M.R. and Halperin, W.E. (1999) Blood lead levels among children of lead-exposed workers: A meta-analysis. Am. J. Ind. Med., 36, 475-481.   DOI
26 Di Nezio, M.S., Palomeque, M.E. and Fernandez Band, B.S. (2004) A sensitive spectrophotometric method for lead determination by flow injection analysis with on-line preconcentration. Talanta, 63, 405-409.   DOI   ScienceOn
27 Silbergeld, E.K., Waalkes, M. and Rice, J.M. (2000) Lead as a carcinogen: experimental evidence and mechanisms of action. Am. J. Ind. Med., 38, 316-323.   DOI
28 Heo, Y., Lee, W.T. and Lawrence, D.A. (1998) Differential effects of lead and cAMP on development and activities of Th1- and Th2-lymphocytes. Toxicol. Sci., 43, 172-185.