• Title/Summary/Keyword: L2 Trigger

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Event Trigger Generator for Gravitational-Wave Data based on Hilbert-Huang Transform

  • Son, Edwin J.;Chu, Hyoungseok;Kim, Young-Min;Kim, Hwansun;Oh, John J.;Oh, Sang Hoon;Blackburn, Lindy;Hayama, Kazuhiro;Robinet, Florent
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.2
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    • pp.55.4-56
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    • 2015
  • The Hilbert-Huang Transform (HHT) is composed of the Empirical Mode Decomposition (EMD) and the Hilbert Spectral Analysis (HSA). The EMD decomposes any time series data into a small number of components called the Intrinsic Mode Functions (IMFs), compared to the Discrete Fourier Transform which decomposes a data into a large number of harmonic functions. Each IMF has varying amplitude and frequency with respect to time, which can be obtained by HSA. The time resolution of the modes in HHT is the same as that of the given time series, while in the Wavelet Transform, Constant Q Transform and Short-Time Fourier Transform, there is a tradeoff between the resolutions in frequency and time. Based on the time-dependent amplitudes of IMFs, we develop an Event Trigger Generator and demonstrate its efficiency by applying it to gravitational-wave data.

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Effects of IAA on the Elongation and Cell Wall Glycosidase Activities in Excised Rape (Brassica napus L. cv. Yongdang) Hypocotyl Segments (유채 하배축 분절의 신장과 세포벽 분해효소의 활성에 미치는 IAA의 효과)

  • Jun, Sung-Soo
    • Journal of Plant Biology
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    • v.27 no.2
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    • pp.43-50
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    • 1984
  • Effects of IAA on the elongation and cell wall hlysocidase activities were investigated in excised rape (Brassica napus L. cv. Yongdang) hypocotyl segments. IAA promoted the elongation of rape hypocotyl segments. In rape hypocotyls, the first 10-mm segments from the hook exhibited maximal elongation and the capacity of elongation was gradually decreased with increasing distance of each 10-mm from the hook. A good correlation has been obtained between the magnitude of endogenous growth and the activities of $\alpha$, $\beta$-glucosidase and $\alpha$, $\beta$-galactosidase. However, exogenous application of IAA did not seem to enhance the tissue with IAA resulted in acidification of the incubation medium. From these data, we can conclude that IAA seems to enhance elongation of the tissue segments, at least in part, by releasing hydrogen ion into cell wall, some of which may participate in the cell wall extension process, but does not seem to trigger the activation of $\alpha$, $\beta$-glucosidase and $\alpha$, $\beta$-galactosidase.

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Action Spectra of Apoptosis Induction and Reproductive Cell Death in L5178Y cells in UV-B Region

  • Mizuho Aoki;Yoshiya Furusawa;Higashi, Sho-ichi;Masakatsu Watanabe
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.454-456
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    • 2002
  • It is important to determine the action spectrum of UV-B radiation contained in the sunlight to estimate the risk of skin cancer. We have investigated action spectra for induction of apoptosis and reproductive cell death in L5178Y cells using the Okazaki Large Spectrograph at NIBB. L5178Y cells were exposed to light at different wavelengths in UV-B or UV-A region. Frequencies of apoptosis induction and reproductive cell death were determined by counting cells with chromatin condensation, and by the colony formation assay, respectively. The measured sensitivity spectra for the two end-points were in very good agreement. Sensitivity decreased steeply with increase of wavelength in UV-B region and remains nearly constant in UV-A region. The action spectra were also slightly steeper than that for the minimum erythematic dose (MED), but very similar to the light absorption spectrum of DNA in UV-B region. On the other hand, the spectra for both endpoints were similar to MED spectrum but not DNA spectrum in the UV-A region. Also different time-course and morphological difference of apoptosis were found between UV-B (long time, fragmentation) and UV-A (short time, shrinkage) region. These results suggest that DNA damage induced by UV-B light triggers apoptosis and reproductive cell death, but other damaged targets (membrane, protein and so on) trigger these effects in UV-A region.

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The Effect of Trigonella foenum-graceum L. (Fenugreek) Towards Collagen Type I Alpha 1 (COL1A1) and Collagen Type III Alpha 1 (COL3A1) on Postmenopausal Woman's Fibroblast

  • Yusharyahya, Shannaz Nadia;Bramono, Kusmarinah;Sutanto, Natalia Rania;Kusuma, Indra
    • Natural Product Sciences
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    • v.25 no.3
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    • pp.208-214
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    • 2019
  • Trigonella foenum-graceum L. (fenugreek) is a phytoestrogen, a nonsteroidal organic chemical compound from plants which has similar mechanism of action to sex hormone estradiol-$17{\beta}$. This study aims to assess the effectivity of fenugreek seeds extract on collagen type I alpha 1 (COL1A1) and collagen type III alpha 1 (COL3A1) which are both decreased in aging skin and become worsen after menopause. This in vitro experimental study used old human dermal fibroblast from leftover tissue of blepharoplasty on a postmenopausal woman (old HDF). As a control of the fenugreek's ability to trigger collagen production, we used fibroblast from preputium (young HDF). Subsequent to fibroblast isolation and culture, toxicity test was conducted on both old and young HDF by measuring cell viability on fenugreek extract with the concentration of 5 mg/mL to $1.2{\mu}g/mL$ which will be tested on both HDF to examine COL1A1 and COL3A1 using ELISA, compared to no treatment and 5 nM estradiol. Old HDF showed a 4 times slower proliferation compared to young HDF (p<0.05). Toxicity test revealed fenugreek concentration of $0.5-2{\mu}g/mL$ was non-toxic to both old and young HDF. The most significant fenugreek concentration to increase COL1A1 and COL3A1 secretion was $2{\mu}g/mL$ (p<0.05).

Link-layer Assisted Seamless Media Streaming over Mobile IP-enabled Wireless LAN (Mobile IP 지원 무선 랜 상에서 링크 계층의 지원을 통한 연속적인 미디어 스트리밍)

  • Lee, Chul-Ho;Lee, Dong-Wook;Kim, Jong-Won
    • Journal of KIISE:Computing Practices and Letters
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    • v.15 no.9
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    • pp.626-636
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    • 2009
  • In Mobile IP-enabled wireless LAN (WLAN), packet flows are corrupted due to the handoff of a mobile node (MN) at the link and network layers, which results in burst packet losses and can cause temporary buffer underflow in a streaming client at the MN. This transient behavior hurts time-sensitive streaming media applications severely. Among many suggestions to address this handoff problem, few studies are concerned with empirical issues regarding the practical validation of handoff options on the time-sensitive streaming media applications. In this paper, targeting seamless streaming over Mobile IP-enabled WLAN, we introduce a seamless media streaming framework that estimates accurate pre-buffering level to compensate the handoff latency. In addition, we propose a link-layer (L2) assisted seamless media streaming system as a preliminary version of this framework. The proposed system is designed to reduce the handoff latency and to overcome the playback disruption from an implementation viewpoint. A packet buffering and forwarding mechanism with L2 trigger is implemented to reduce the handoff latency and to eliminate burst packet losses generated during the handoff. A pre-buffering adjustment is also performed to compensate the handoff latency. The experimental results show that the proposed approach eliminates packet losses during the handoff and thus verify the feasibility of seamless media streaming over Mobile IP-enabled WLAN.

Immunoinformatics studies and design of a novel multi-epitope peptide vaccine against Toxoplasma gondii based on calcium-dependent protein kinases antigens through an in-silico analysis

  • Ali Dalir Ghaffari;Fardin Rahimi
    • Clinical and Experimental Vaccine Research
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    • v.13 no.2
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    • pp.146-154
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    • 2024
  • Purpose: Infection by the intracellular apicomplexan parasite Toxoplasma gondii has serious clinical consequences in humans and veterinarians around the world. Although about a third of the world's population is infected with T. gondii, there is still no effective vaccine against this disease. The aim of this study was to develop and evaluate a multimeric vaccine against T. gondii using the proteins calcium-dependent protein kinase (CDPK)1, CDPK2, CDPK3, and CDPK5. Materials and Methods: Top-ranked major histocompatibility complex (MHC)-I and MHC-II binding as well as shared, immunodominant linear B-cell epitopes were predicted and linked using appropriate linkers. Moreover, the 50S ribosomal protein L7/L12 (adjuvant) was mixed with the construct's N-terminal to increase the immunogenicity. Then, the vaccine's physicochemical characteristics, antigenicity, allergenicity, secondary and tertiary structure were predicted. Results: The finally-engineered chimeric vaccine had a length of 680 amino acids with a molecular weight of 74.66 kDa. Analyses of immunogenicity, allergenicity, and multiple physiochemical parameters indicated that the constructed vaccine candidate was soluble, non-allergenic, and immunogenic, making it compatible with humans and hence, a potentially viable and safe vaccine candidate against T. gondii parasite. Conclusion: In silico, the vaccine construct was able to trigger primary immune responses. However, further laboratory studies are needed to confirm its effectiveness and safety.

The Study on Apoptosis and Expression of Fas, Fas-ligand, Bax, and Bcl-2 in Human Fragmented Embryos (분절화된 인간 배아에서 세포자연사와 Fas, Fas-ligand, Bax, Bcl-2 발현에 관한 연구)

  • Kim, Jong-Sik;Kim, Myoung-Shin;Yang, Hyun-Won;Yu, Chai-Hyeock;Yoon, Yong-Dal;Bae, In-Ha;Jung, Byeong-Jun;Song, Hyun-Jin
    • Clinical and Experimental Reproductive Medicine
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    • v.29 no.3
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    • pp.167-178
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    • 2002
  • Objective : The present study was performed to investigate whether apoptosis occur in human embryos by annexin staining and detect the expression of Fas, Fas-ligand (FasL), Bax, and Bcl-2 in human fragmented embryos derived from IVF-ET by immunofluorescence and Western blot analysis. Materials and Methods: Using annexin staining, immunofluorescence and Western blot analysis on normal and fragmented embryos, we were able to detect apoptotsis and apoptotic gene products in fragmented embryos. Result: Phosphatidylserine (PS) translocation, the marker for apoptosis, were detected frequently in fragmented embryos. Bcl-2 and Bax protein were detected in both fragmented and non-fragmented embryos. When fragmented embryos compared to normal embryos, immunofluorescent intensity of Bcl-2 tended to be lower in fragmented embryos. Bax gene expression increased in the fragmented embryos compared to the normal embryos. This result supports a model in which the molar ratio of Bcl-2 to Bax determines whether apoptosis induced or inhibited in human embryo. Fas was highly expressed in human preimplantation embryos but not FasL. It suggests that embryo may undergo apoptosis by binding with FasL produced by follicular or immune cells. Conclusion: The over expression of Bax and Fas will trigger apoptosis to lead embryo fragmentation and change embryo to be nonviable.

The Relationship of the L-type $Ca^{2+}$ Channel on the Depolarization-and Depletion of SR $Ca^{2+}$ -induced Smooth Muscle Contraction and Intracellular $Ca^{2+}$ Mobilization (탈분극과 근장그물 내 $Ca^{2+}$ 고갈-유도 평활근의 수축 및 세포 내 $Ca^{2+}$ 변동에 관여하는 L-형 $Ca^{2+}$ 통로의 상관성)

  • Kim, Jung-Hwan
    • The Journal of Korean Physical Therapy
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    • v.19 no.5
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    • pp.65-76
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    • 2007
  • Purpose: It is generally accepted that smooth muscle contraction is triggered by intracellular $Ca^{2+}$ ($[Ca^{2+}]_i$) released from intracellular $Ca^{2+}$ stores such as sarcoplasmic teticulum (SR) and from the extracellular space. The increased $[Ca^{2+}]^i$ can phosphorylate the 20,000 dalton myosin light chain $(MLC_{20})$ by activating MLC kinase (MLCK), and this initiates smooth muscle contraction. In addition to the $[Ca^{2+}]_i$MACK-tension pathway, a number of intracellular signal molecules, including mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and others, play important roles in the regulation of smooth muscle contraction. However, the mechanisms regulating contraction of depletion of SR $Ca^{2+}$ in mouse gastric smooth muscle strips is not still clear. Methods: To investigate the rotes of $Ca^{2+}$ influx and SR $Ca^{2+}$ release channel on gastric motility, isometric contraction and $[Ca^{2+}]_i$ were examined in mouse gastric smooth muscle strips. Results: High KCl, ryanodine, an activator of $Ca^{2+-}$induced $Ca^{2+}$ release channel, and cyclopiazonic acid (CPA), an inhibitor of SR $Ca^{2+-}$ATPase evoked a sustained increase in muscle contraction and $[Ca^{2+}]_i$. These increases induced by high KCl, ryanodine, and CPA were partially blocked by application of verapamil ($10{\mu}M$), a L-type $Ca^{2+}$ channel inhibitor. Additionally, in $Ca^{2+-}$free solution (1 mM EGTA), ryanodine and CPA had no effect contraction and $[Ca^{2+}]_i$ in fundic muscle strips. Conclusion: These results that extracellular $Ca^{2+}$ influx and depletion of SR trigger $Ca^{2+}$ influx through verapamil-sensitive $Ca^{2+}$ channel, and extracellular and SR $Ca^{2+}$ store may functionally involve in the subcellular $Ca^{2+}$ mobilization in mouse gastric muscle.

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A hierarchical Xcast++ mechanism for multicast services in mobile communication environment (이동 통신망 환경에서 멀티캐스트를 제공하기 위한 계층적 Xcast++ 기법)

  • Kim Tae-Soo;Lee Kwang-Hui
    • Journal of Internet Computing and Services
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    • v.6 no.3
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    • pp.55-70
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    • 2005
  • In order to provide mobile hosts with multicast service in mobile communication environment, we proposed a multicast mechanism named HXcast++ which is an extended version of the existing Xcast++ with hierarchical architecture, We assured that mobile hosts could get multicast service through an optimal path regardless of their location by making DR(Designated Router) join a group on behalf of the mobile hosts, In this present research we introduced hierarchical architecture in order to reduce the maintenance cost resulting from frequent handoff. We also proposed a GMA (Group Management Agent) based group management mechanism which enables the mobile hosts to join the group without waiting for a new IGMP Membership Query. A fast handoff method with L2 Mobile Trigger was, in this work, employed in order to reduce the amount of the packet loss which occurs as a result of the handoff, We also managed to curtail the packet loss caused by the latency of the group join by using a buffering and forward mechanism.

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Effect of Coptidis Rhizoma Extract on Cytokine Production of Mouse Macrophages (황연(黃連) 추출물이 대식세포의 면역단백질 생성에 미치는 영향)

  • Kim, Bok-Kee;Han, Hyo-Sang;Lee, Young-Jong
    • Herbal Formula Science
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    • v.21 no.2
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    • pp.81-89
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    • 2013
  • Objectives : The purpose of this study is to observe the effect of Coptidis Rhizoma (CCE-extract of C. chinensis Rhizome) in induction of immune protein on mouse macrophages. Methods : To analyze cytokines interleukin(IL)-$1{\alpha}$, IL-3, IL-9, IL-12p40, IL-13, IL-17, Monocyte Chemoattractant Protein(MCP)-1 induced by macrophages, mouse macrophages were incubated with CCE and was measured. Results : IL-$1{\alpha}$ measurement, CCE showed significant inhibition only at concentration level of 200 ${\mu}g/mL$. IL-3, MCP-1 measurement, CCE showed significant inhibition only at concentration level of 100, 200 ${\mu}g/mL$. IL-9 measurement, CCE showed significant inhibition only at concentration level of 50 ${\mu}g/mL$. IL-13 measurement, CCE showed significant inhibition only at concentration level of 50, 100, 200 ${\mu}g/mL$. The IL-12p40, IL-17 levels indicated no changes at 25, 50, 100, 200 ${\mu}g/mL$ on mouse macrophages. Conclusions : CCE did not significantly increased inflammatory cytokines IL-$1{\alpha}$, IL-3, IL-9, IL-12p40, IL-13, IL-17, Monocyte Chemoattractant Protein(MCP)-1 on mouse macrophages. It was verified CCE does not trigger cytokine related hypersensitivity reaction of organism or exacerbation of acute/chronic inflammatory disease.