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The Study on Apoptosis and Expression of Fas, Fas-ligand, Bax, and Bcl-2 in Human Fragmented Embryos  

Kim, Jong-Sik (Infertility Research Laboratory, Seoul Women's Hospital)
Kim, Myoung-Shin (Infertility Research Laboratory, Seoul Women's Hospital)
Yang, Hyun-Won (Department of OB/GYN, Eulji University School of Medicine)
Yu, Chai-Hyeock (Department of Life Science, College of Natural Sciences, Inha University)
Yoon, Yong-Dal (Department of Life Science, College of Natural Sciences, Hanyang University)
Bae, In-Ha (Department of Biology, College of Natural Sciences, Sungsin Women's University)
Jung, Byeong-Jun (Infertility Clinic, Seoul Women's Hospital)
Song, Hyun-Jin (Infertility Clinic, Seoul Women's Hospital)
Publication Information
Clinical and Experimental Reproductive Medicine / v.29, no.3, 2002 , pp. 167-178 More about this Journal
Abstract
Objective : The present study was performed to investigate whether apoptosis occur in human embryos by annexin staining and detect the expression of Fas, Fas-ligand (FasL), Bax, and Bcl-2 in human fragmented embryos derived from IVF-ET by immunofluorescence and Western blot analysis. Materials and Methods: Using annexin staining, immunofluorescence and Western blot analysis on normal and fragmented embryos, we were able to detect apoptotsis and apoptotic gene products in fragmented embryos. Result: Phosphatidylserine (PS) translocation, the marker for apoptosis, were detected frequently in fragmented embryos. Bcl-2 and Bax protein were detected in both fragmented and non-fragmented embryos. When fragmented embryos compared to normal embryos, immunofluorescent intensity of Bcl-2 tended to be lower in fragmented embryos. Bax gene expression increased in the fragmented embryos compared to the normal embryos. This result supports a model in which the molar ratio of Bcl-2 to Bax determines whether apoptosis induced or inhibited in human embryo. Fas was highly expressed in human preimplantation embryos but not FasL. It suggests that embryo may undergo apoptosis by binding with FasL produced by follicular or immune cells. Conclusion: The over expression of Bax and Fas will trigger apoptosis to lead embryo fragmentation and change embryo to be nonviable.
Keywords
Apoptosis; Fas; Fas-ligand (FasL); Bax; Bcl-2; Human fragmented embryos;
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