• 한국초지조사료학회지
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• 제43권1호
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• pp.56-61
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• 2023

The germination process is critical for plant growth and development and it is largely affected by environmental stress, especially salinity. Recently, hydrogen sulfide (H₂S) is well known to act as a signaling molecule in a defense mechanism against stress conditions but poorly understood regulating seed germination. In this study, the effects of NaHS (the H₂S donor) pretreatment on various biochemical (hydrogen peroxide (H₂O₂) content and amylase and protease activity) and physiological properties (germination rate) during seed germination of oilseed rape (Brassica napus L. cv. Mosa) were examined under salt stress. The seed germination and seedling growth of oilseed rape were inhibited by NaCl treatment but it was alleviated by NaHS pretreatment. The NaCl treatment increased H₂O₂ content leading to oxidative stress, but NaHS pre-treatments maintained much lower levels of H₂O₂ in germinating seeds under salt stress. Amylase activity, a starch degradation enzyme, significantly increased over 2-fold in control, NaHS pretreatment, and NaHS pretreatment under NaCl during seed germination compared to NaCl treatment. Protease activity was highly induced in NaHS-pretreated seeds compared to NaCl treatment, accompanied by a decrease in protein content. These results indicate that NaHS pretreatment could improve seed germination under salt stress conditions by decreasing H₂O₂ accumulation and activating the degradation of protein and starch to support seedling growth.

• Journal of Korean Biological Nursing Science
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• 제25권1호
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• pp.32-42
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• 2023

Purpose: This study aimed to investigate the association between obesity and high-sensitivity C-reactive protein (hs-CRP) levels in Korean adults without cardiovascular disease (CVD). Methods: The subjects were 3,634 adults, and data were extracted from the seventh Korea National Health and Nutrition Examination Survey (KNHANES VII-3). A complex sampling design analysis was applied to reflect the stratified and clustered weights. The data were analyzed using the complex sample Rao-Scott chi-square test and multiple logistic regression analysis (in SPSS for Windows version 26.0). Obesity, according to body mass index (BMI), was defined as obesity (BMI = 25-29.9 kg/m²), high obesity (BMI = 30-34.9 kg/m²), and super-high obesity (BMI ≥ 35 kg/m²), and abdominal obesity (AO) was defined as a waist circumference (WC) ≥ 90 cm in males and WC ≥ 85 cm in females. Results: The odds ratios for moderate CVD risk (hs-CRP; 1-3 mg/dL) were 2.21, 4.16, and 7.13 in the obesity, high obesity, and super-high obesity groups, respectively, compared to the normal BMI group. The odds ratio for moderate CVD risk was 2.18 in males with AO and 1.88 in females with AO. The odds ratios for high CVD risk (hs-CRP > 3 mg/L) were 4.40 and 17.55 in the high obesity and super-high obesity groups, respectively, compared to the normal BMI group. Conclusion: This study provides evidence that early detection and prevention programs for CVD should include obesity-related interventions aiming to modulate hs-CRP.

• Weed & Turfgrass Science
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• 제7권3호
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• pp.259-268
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• 2018

본 연구는 부식산과 효모균(Saccharomyces cerevisiae) 함유 액비(LHS)의 시비에 따른 잔디의 생육과 품질의 변화를 확인하기 위해 잔디의 가시적 품질, 엽록소 함량, 예지물량, 양분 함량 및 흡수량을 조사하였다. 처리구는 대조구(CF), LHS 1,000배 처리구(HS-1), LHS 500배 처리구(HS-2) 및 LHS 250배 처리구(HS-3)로 구분되었다. LHS 처리 후 토양 pH는 감소하여 약산성을 나타내었다. LHS 처리구와 대조구를 비교하였을 때, 가시적 품질, 엽록소 함량 및 양분 함량 등은 통계적으로 유의적인 차이를 나타내지 않았고, 잔디 예지물량이 증가되었고, 인 흡수량은 HS-2 처리구에서 각각 22%와 33%씩 증가하였다. 이 결과들을 종합해 볼 때, 크리핑 벤트그래스에서 부식산과 효모균 함유 액비의 시비는 500배 희석액 처리 시 인의 흡수를 촉진하고, 잔디의 지상부 생장을 증가시키는 것을 확인 할 수 있었다.

• 분석과학
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• 제17권6호
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• pp.443-453
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• 2004

톨루엔은 환각성 유기용매로서 청소년에 의하여 남용되는 본드류에서 주성분으로 사용되고 있다. 본 연구에서는 Headspace 가스크로마토그래프 방법에 의한 톨루엔 남용자들의 혈액, 소변, 타액에서 톨루엔을 분석하는 방법을 확립하기 위하여 측정 방법의 유효화와 측정에 관련된 불확도를 추정하였다. 혈액, 소변, 타액 시료 중에서 검출한계는 타액에서 $0.01{\mu}g/mL$로, 정량한계는 혈액에서 $0.1{\mu}g/mL$로 가장 높았다 (N=5). 각 생체시료에 대한 유효화의 다른 주요 인자들인 직선성, 분석농도범위, 정확도, 정밀도, 회수율 등도 측정하였다. 톨루엔 $1{\mu}g/mL$의 농도에서 혈액, 소변, 타액 시료의 톨루엔 분석방법에 대한 불확도는 각각 0.13, 0.09, $0.17{\mu}g/mL$로 추정되었고, 추정된 불확도의 성분들 중에서 낮은 농도에서는 검량선에 대한 불확도가 가장 큰 비중을 차지하고 있었다.

• Journal of Microbiology and Biotechnology
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• 제30권1호
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• pp.136-145
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• 2020

Chlorella sp. HS2, which previously showed excellent performance in phototrophic cultivation and has tolerance for wide ranges of salinity, pH, and temperature, was cultivated heterotrophically. However, this conventional medium has been newly optimized based on a composition analysis using elemental analysis and ICP-OES. In addition, in order to maintain a favorable dissolved oxygen level, stepwise elevation of revolutions per minute was adopted. These optimizations led to 40 and 13% increases in the biomass and lipid productivity, respectively (7.0 and 2.25 g l^-1d^-1 each). To increase the lipid content even further, 12 h heat shock at 50℃ was applied and this enhanced the biomass and lipid productivity up to 4 and 17% respectively (7.3 and 2.64 g l^-1d^-1, each) relative to the optimized conditions above, and the values were 17 and 14% higher than ordinary lipid-accumulating N-limitation (6.2 and 2.31 g l^-1d^-1). On this basis, heat shock was successfully adopted in novel Chlorella sp. HS2 cultivation as a lipid inducer for the first time. Considering its fast and cost-effective characteristics, heat shock will enhance the overall microalgal biofuel production process.

• 한국축산식품학회지
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• 제36권4호
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• pp.469-475
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• 2016

This study examined the relationship between NaCl sensitivity and stress response of Listeria monocytogenes. Nine strains of L. monocytogenes (NCCP10805, NCCP10806, NCCP10807, NCCP10808, NCCP10809, NCCP10810, NCCP10811, NCCP10920 and NCCP 10943) were exposed to 0%, 1%, 2% and 4% NaCl, and then incubated at 60℃ for 60 min to select strains that were heat-sensitized (HS) and non-sensitized (NS) by NaCl exposure. After heat challenge, L. monocytogenes strains were categorized as HS (NCCP 10805, NCCP10806, NCCP10807, NCCP10810, NCCP10811 and NCCP10920) or NS (NCCP10808, NCCP10809 and NCCP10943). Total mRNA was extracted from a HS strain (NCCP10811) and two NS strains (NCCP10808 and NCCP10809), and then cDNA was prepared to analyze the expression of genes (inlA, inlB, opuC, betL, gbuB, osmC and ctc) that may be altered in response to NaCl stress, by qRT-PCR. The expression levels of two invasion-related genes (inlA and inlB) and two stress response genes (opuC and ctc) were increased (p<0.05) in NS strains after NaCl exposure in an NaCl concentration-dependent manner. However, only betL expression was increased (p<0.05) in the HS strains. These results indicate that the effect of NaCl on heat sensitization of L. monocytogenes is strain dependent and that opuC and ctc may prevent NS L. monocytogenes strains from being heat sensitized by NaCl. Moreover, NaCl also increases the expression of invasion-related genes (inlA and inlB).

• The Korean Journal of Pain
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• 제32권3호
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• pp.196-205
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• 2019

Background: Hypertonic saline (HS) injections for decompressive neuroplasty (DN) can cause pain. We assessed whether a continuous infusion of HS through an infusion pump would reduce injection-related pain compared with repeated bolus administrations. Methods: Fifty patients scheduled for DN were randomized to either the bolus injection or the continuous infusion group. After appropriately placing the epidural catheter, 4 mL of 5% NaCl was injected as four boluses of 1 mL each at 15-minute intervals or infused over 1 hour using an infusion pump. The severity of pain induced by HS injection, as measured by the 11-point numerical rating scale (NRS), was the primary outcome. The severity of low back or lower extremity pain, as measured by the 11-point NRS and Oswestry Disability Index (ODI), 3 months following the procedure, was the secondary outcome. Results: Data from 21 patients in the bolus group and 23 in the continuous infusion group were analyzed. No statistically significant difference in injection-related pain was identified between the two groups during the initial HS administration (P = 0.846). However, there was a statistically significant reduction in injection-related pain in the continuous infusion group compared to the bolus injection group from the second assessment onwards (P = 0.001, < 0.001, and < 0.001, respectively). No significant between-group differences in the NRS and ODI scores 3 months post-procedure were noted (P = 0.614 and 0.949, respectively). Conclusions: Our study suggests that administering HS through a continuous infusion is a useful modality for reducing HS injection-related pain during DN.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.246.1-246.1
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• 2002

We studied the effects of ursodeoxycholic acid (UDCA) and its synthetic derivatives. HS-l030 and HS-1183. and chenodeoxycholic acid (CDCA) and its synthetic derivatives, HS-1199 and HS-1200. on the human colon adenocarcinoma cell line. HT -29 (p53 mutant type). The effects on cell viability and growth were assessed by MTT assay and cell growth study. While UDCA and CDCA exhibited no significant effect, their novel derivatives inhibited the proliferation of HT-29 cell line in a concentration- and time-dependent manners. (omitted)

• 대한환경공학회지
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• 제27권3호
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• pp.240-246
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• 2005

오존 및 입상활성탄 공정으로 구성된 고도정수처리시설을 대상으로 공정유입수인 모래여과수를 포함하는 각각의 공정수로부터 휴믹물질(HS; humic substances)을 분리 및 추출하여 작용기 분포, 화학적 조성, FT-IR(Fourier transform infrared) 그리고 $^1H$-NMR(proton nuclear magnetic resonance) 스펙트럼을 분석하여 구조 및 화학적 특성을 평가하였다. 오존처리에 이어 입상활성탄 흡착처리를 거치면서 휴믹성분(humic fraction)의 농도분포는 36.3%에서 24.2%로 단계적으로 감소하였으며, 모래여과수가 활성탄 흡착칼럼으로 직접 유입되는 공정의 경우 36.3%에서 25.0%로 감소하는 것으로 나타났다. 전오존처리 이후 활성탄 흡착처리시 HS 중 페놀기(phenolic groups)와 카르복실기(carboxylic groups)의 농도분포는 38.4%에서 23.5%로 그리고 61.6%에서 43.3%로 각각 감소하였으며, 전오존처리하지 않은 경우 38.4%에서 24.0%로 그리고 61.6%에서 44.9%로 각각 감소하였다. 정수처리를 거치면서 HS의 카르복실기에 대한 페놀기의 비율에 따라 HS 분자구조 중 O/C 몰비가 감소하거나 또는 증가하는 결과를 나타내었다.

• 대한본초학회지
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• 제28권1호
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• pp.9-14
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• 2013

Objectives： Bletillae Rhizoma, the roots of Bletilla striata, is used to restrain the leakage of blood and stop bleeding. It can cure the sores, ulcers, and chapped skin. This study was designed to investigate the collagen metabolism, elastase and tyrosinase activity of Bletillae Rhizoma extract (BR). Methods : The effects of BR on type I procollagen production and collagenase activity in human normal fibroblasts Hs68 after UVB (312 nm) irradiation were measured by ELISA method. The elastase activity, tyrosinase activity, and L-DOPA oxidation after treatment of BR were measured as well. Results : In the present study, the collagen production (type I procollagen) was significantly increased to $15.7{\pm}1.8$ ng/ml at a concentration of BR 100 ${\mu}g/ml$ in UVB damaged Hs68 cells. The increased collagenase activity after UVB damage was significantly recovered to $42.7{\pm}0.7%$, $54.5{\pm}3.5%$, and $38.4{\pm}0.9%$ by BR 10, 30, and 100 ${\mu}g/ml$. The activities of BR 10 mg/ml on tyrosinase activity was significantly reduced to $45.1{\pm}8.4%$ as well. However, there were no significant effects on the elastase activity and the L-DOPA oxidation. Conclusion : BR showed the promoting effects of collagen synthesis and inhibitory effects of collagenase activity in Hs68, human normal fibroblast cells. And these could be thought to have the anti-wrinkle effects and whitening effects in vitro. These results suggest that BR may have potential as an anti-aging ingredient in cosmetic treatment.