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Evaluation for the simultaneous Removal of Organic Matters and Nutrients by the RBC and tapered Aeration Processes with Bacillus sp. for the high Strength of Dairy Wastewater (바실러스 미생물을 이용한 고농도 유가공 폐수처리에 있어서 유기물질과 영양염류의 동시제거에 대한 평가)

  • Lee, Sang-Ho
    • Journal of Korean Society of Water and Wastewater
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    • v.24 no.2
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    • pp.195-202
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    • 2010
  • The evaluation of organic matters and nutrients removal was investigated for the synthetic wastewater and the high strength of dairy wastewater. Two different systems were performed for this research. System A composing of a single RBC with tapered aeration was fed with the synthetic wastewater for 74 days with 173L/day of influent, 200% of internal return and 100% of sludge return for the period 1 and 2. The feed conditions were maintained 346L/day of influent, 50% of internal return and sludge return for the period 3. The dairy wastewater was introduced to evaluate treatment efficiency for system B composing of dual RBCs and tapered aeration tanks for 50 days of experimental run time, in which hydraulic rates were maintained at the constant ratios of 346L/day, 50% of internal return and 50% of sludge return. The spiral string media made of nylon fibre was attached by Bacillus sp. in RBC for both systems. The specific area of string media was $1.4m^2$/m and biomass was maintained at the concentrations of 23g/m. The synthetic wastewater was supplied by 1,800mg/L of glucose, 500mg/L of $NH_4Cl$, and 500mg/L of $KH_2PO_4$ to system A. The dairy wastewater was supplied to system B from dairy production plant. The average influent concentrations were 1,334mg/L of BOD, 2,014mg/L of CODcr, 160mg/L of T-N, and 12mg/L of T-P in system A. The average influent concentrations of parameters were 1,006 mg/L for BOD, 1,875mg/L for $COD_{cr}$, 51.6mg/L for T-N and 8.9mg/L for T-P in system B. Results indicated that removal efficiencies of BOD and $COD_{cr}$ were more than 90% however, the removal efficiency of T-N was 87%, and that of T-P was 82% for system A. Removal efficiencies were 98.5% of BOD, 91.3% of nitrogen and 89% of phosphorus for system B. The removal efficiencies of organic matters, T-N and T-P were higher in system B than in system A. The effluent quality issued by the stringent national legislations for the discharge of the high strength of dairy products wastewater can be improved using sequential RBCsand tapered aeration reactors rather than a single RBC and tapered aeration reactors with Bacillus sp.

Baby-Step 2k-ary Adult-Step Algorithm for Symmetric-Key Decryption (대칭키 해독을 위한 아기걸음 2k-ary 성인걸음 알고리즘)

  • Lee, Sang-Un
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.15 no.2
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    • pp.23-29
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    • 2015
  • The baby-step giant-step algorithm seeks b in a discrete logarithm problem when a,c,p of $a^b{\equiv}c$(mod p) are already given. It does so by dividing p by m block of $m={\lceil}{\sqrt{p}}{\rceil}$ length and letting one giant walk straight toward $a^0$ with constant m strides in search for b. In this paper, I basically reduce $m={\lceil}{\sqrt{p}}{\rceil}$ to p/l, $a^l$ > p and replace a giant with an adult who is designed to walk straight with constant l strides. I also extend the algorithm to allow $2^k$ adults to walk simultaneously. As a consequence, the proposed algorithm quarters the execution time of the basic adult-walk method when applied to $2^k$, (k=2) in the range of $1{\leq}b{\leq}p-1$. In conclusion, the proposed algorithm greatly shorten the step number of baby-step giant-step.

Measurement of Lactoferrin, IgA, IgG1, IgG2, Antibacterial Activity, and Lactic Acid Bacterial Growth in Holstein Colostrum (Holstein 초유 중 Lactoferrin, IgA, IgG1, IgG2 정량과 미생물의 성장에 미치는 영향)

  • Renchinthand, Gereltuya;Bae, Hyoung-Churl;Nam, Myoung-Soo
    • Food Science of Animal Resources
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    • v.27 no.4
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    • pp.522-530
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    • 2007
  • This experiment was carried out to measure the content of lactoferrin, IgA, $IgG_1,\;IgG_2$, in Holstein colostrum, and to test the effect of it's colostrum on the antibacterial activity to pathogenic bacteria and the growth stimulation of lactic acid bacteria. Colostrum was collected at the first, second, and third day after parturition in summer and winter season. The levels of lactoferrin, IgA, $IgG_1,\;and\;IgG_2$ in Holstein cow colostrum were 0.30 mg/mL, 0.37 mg/mL, 4.00 mg/mL, 0.37 mg/mL, respectively, on the first day of the summer season whereas they were 1.16 mg/mL, 2.60 mg/mL, 13.35 mg/mL, 1.30 mg/mL on the first day of the winter season, postpartum. Heat treated ($65^{\circ}C$ for 30 min) or non-treated colostrum showed antibacterial activity toward Escherichia coli. The growth of commercial mixed strains (Bifidobacterium longum, Lactobacillus acidophilus, Streptococcus themophilus), L. acidophilus, L. casei, L. bulgaricus, and L. lactis subsp. cremoris were improved in first, second and third day colostrum compared to normal milk. Commercial miked strains (B. longum, L. acidophilus S. themophilus) lowered the pH to 4.97-5.22 and 4.89 while increasing the titratable acidity to 0.75-0.88% and 0.70% in colostrum and normal milk, respectively. However, L. casei, L. bulgaricus, L. lactis subsp. cremoris lowered the pH to 5.96-6.47 and 6.5-6.8 while increasing the titratable acidity to 0.29-0.48% and 0.20-0.25% in colostrum and normal milk, respectively.

Crystal Structure of Three-Dimensional Nickel(II) Tetraaza Macrocyclic Complex Linked by Hydrogen-Bonds (수소 결합에 의한 이차원의 Nickel(II) Tetraaza 거대 고리 착물 결합구조)

  • Park, Ki-Young;Choo, Geum-Hong;Suh, Il-Hwan
    • Korean Journal of Crystallography
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    • v.13 no.1
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    • pp.12-16
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    • 2002
  • The complex [Ni(L)](BDC)·4H₂O (1) (L = 3,14-dimethyl-2,6,13,17-tetraazatricyclo[16,4,O/sup 1.18/,O/sup 7.12/] docosane; BDC = 1,3-benzenedicarboxylate) has been synthesized and characterized by X-ray crystallography. Compound 1 crystallizes in the orthorhombic space group Pcnb, with a = 8.764(2) , b = 17.687(2) , c = 19.475(1) , V = 3018.7(8) ³, Z = 4, R₁, (wR₂) for 2148 observed reflections of [1>2σ(I) was 0.0822 (0.2236). Compound 1 is interconnected to give a three-dimensional network through weak hydrogen-bonding interactions.

Synthesis of (${\pm}$)-cis-8-amino-l-2,3,4,4a,5,10b-hexahydrothiazolo[4,5-f]indeno [1,2-b][1,4]oxazine ((${\pm}$)-cis-8-Amino-2,3,4,4a,5,10b-hexahydrothiazolo[4,5-f]indeno [1,2-b][1,4]oxazine의 합성)

  • Ma, Eun-Sook
    • YAKHAK HOEJI
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    • v.52 no.6
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    • pp.488-493
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    • 2008
  • 2-Aminothiazole ring as a bioisoster of catechol in dopamine has provided with good oral availability and lipophilic property. 2-Aminoindan, is a rigid form of dopamine, was evaluated as a dopamine D3 agonist with low neurotoxicity. Dopamine D3 agonist was evaluated as selective for the treatment of Parkinson's disease. In order to develop a novel dopamine D3 agonist, we tried to synthesize the aminothiazoloindenoxazine derivative that is a hybrid structure of aminoindenoxazine and thiazole ring. cis-2-Amino-1-indanol (2) was synthesized from 1,2-indandione-2-oxime by catalytic hydrogenation and it was treated with chloroacetyl chloride and NaH in benzene solution to give (${\pm}$)-cis-4,4a,5,9b-tetrahydroindeno[1,2-b][1,4]oxazin-3(2H)-one (6). Nitration of 6 by the mixed acid gave 8-nitro compound (7) and the carbonyl group of 7 was reduced with $LiAlH_4$ to afford compound (8). 8 was reduced to form (${\pm}$)-cis-8-amino-2,3,4,4a,5,9b-hexahydroindeno[1,2-b][1,4]oxazine (9) and finally it was cyclized with KSCN in glacial acetic acid to yield (${\pm}$)-cis-8-amino-2,3,4,4a,5,10b-hexahydrothiazolo[4,5-f]indeno[1,2-b][1,4]oxazine (10).

The Design of Miniature Microstrip Fractal Patch Antenna Using L-shaped Feeding Structure. (L-형급전구조를 이용한 소형 마이크로스트립 Fractal 패치 안테나 설계)

  • Park, Chang-Hyun;Yoon, Chi-Mu;Kim, Kab-Ki
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2007.10a
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    • pp.863-867
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    • 2007
  • In this paper, We have designed miniature microstrip patch antenna based on fractal structure using L-shaped feeding structure for 4G mobile communication applications. Miniature antenna has achieved by the presence of unusual fundamental resonant mode which we call "crossed-diagonal"(CD)current. Using CST Microwave Studio 5.0, patch antenna was designed. The simulated input return loss showed the bandwidth of 1.2647[GHz]($2.944{\sim}4.209GHz$), 35.4% below -10dB. The gain of E, H-plane was achieved 8.3dBi and 8.4dBi respectively. And beamwidth of 3dB in the E, H-plane was $40.6^{\circ}$ and $81.6^{\circ}$, respectively.

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Performance of analysis UWB system using MIMO-OFDM and frequency diversity (STBC-OFDM과 주파수 다이버시티를 적용한 UWB 시스템이 성능분석)

  • Choi, Jung-Hun;Han, Tae-Young;Kim, Nam
    • Proceedings of the Korea Contents Association Conference
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    • 2004.05a
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    • pp.423-428
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    • 2004
  • In this paper, STBC (Space Time Block Code) is applied to the WB system and frequency repeated diversity is used to get the 4-th order space time diversity gain. The performance of が STDB-OFDM system is analyzed by computer simulation. As a result of simulation, proposed W system can reduce the complexity that is introduced by increasing number of transmit antenna and show the same performance of 4 antennas with only using 2. Proposed system shows the enhancement of 7.1 dB compared to the general UWB OFDM and 1.9 dB compared to UWB STBC-OFDM.

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A Study on Stage Classification of Eight Constitution Questionnaire (팔체질 진단을 위한 단계별 설문지 개발 연구)

  • Lee, Joo-Ho;Kim, Min-Yong;Kim, Hee-Ju;Shin, Young-Sup;Oh, Hwan-Sup;Park, Young-Bae;Park, Young-Jae
    • The Journal of the Society of Korean Medicine Diagnostics
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    • v.16 no.2
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    • pp.59-70
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    • 2012
  • Objectives : Pulse diagnosis by Expert is the only way to classify 8 Constitutions so the study to supplement classifying method by the questionnaire has developed and modified and ECM-32 System has designed in 2010. But analyzing with Decision tree had many nodes and 32 important questions omitted while processing the data. So this study was to classify the 8 constitution patients into 2 groups first and analyze its characters in consecutive order. Methods : The participants of this study were 1027 patients who classified into one of the 8 constitutions according to pulse diagnosis and answered 251 questionnaires in 2010. They were divided into sympathetic nerve acceleration constitution and parasympathetic nerve acceleration constitution and analyzed with decision tree. Results : The reponses of the questionnaire were analyzed with 4 methods of 5 scales interval method from 0 to 5, Na, Low(1,2), Medium(3), High(4,5), average value, Y/N dichotomy. Average Value had no significance. 1. From the 5 scale interval method 6 questionnaires with 7 nodes (F5e, B1d, F7f, F2a, F1b, C4L) were significant. The accuracy was 92.5%. 2. From L, M, H method 7 questionnaires with 7 nodes(F5e, B1d, F7f, F1a, B1c, C4L, P3d) were significant. The accuracy was 92.5%. 3. From Y/N dichotomy 9 questionnaires with 9 nodes( F5e, B1d, F7f, F1a, B1c, C4L, B1b, P1i, B2a) were significant. The accuracy was 93.18%. Conclusions : Based on this study, Yes or No dichotomy method was most significant and categorized among the 4 methods. Unlike previous studies which used interval scale method only, Y/N dichotomy method was more statistically significant with the questionnaire to supplement the method of pulse diagnosis. For further study by analyzing decision tree method in consecutive order, the patients can be divided into 8 Constitutions with higher significance with less questionnaires.

Changes of Oxidative Enzymes and Fatty Acid Composition of Bifidobacterium adolescentis and B. longum under Anaerobic and Aerated Conditions. (산소의 Stress에 따른 Bifidobacterium adolescentis와 Bifidobacterium longum의 산화효소의 활성과 세포 지방산 조성의 변화)

  • 신순영;박종현
    • Microbiology and Biotechnology Letters
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    • v.26 no.1
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    • pp.7-14
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    • 1998
  • To study the oxygen tolerance mechanism of bifidobacteria, we have studied the growth of cells, the activities of the enzymes which were related with oxygen, such as catalase, superoxide dismutase(SOD), NADH oxidase, and NADH peroxidase, and cellular fatty acid compositions of Bifidobacterium adolescentis and B. longum under anaerobic and aerated (microaerobic and aerobic) conditions. B. longum grew relatively well under the microaerobic conditions, whereas the growth of B. adolescentis was inhibited under the same aerated conditions. B. adolescentis had extremely low level of NADH oxidative enzymes while B. longum had the relatively high level of NADH oxidative enzymes, whose activities were dramatically increased from 3.7 to 11.4 times by microaerobic condition but not in B. adolescentis. The activity of SOD was unexpectedly high in B. adolescentis compared with in B. longum under anaerobic and aerated conditions. The activities of catalase were not detected in all samples tested in this study. We also found that normal $C_{l6:0}$ and $C_{18:1}$ were the major fatty acids in B. adolescentis and B. longum under anaerobic and aerated conditions. 2.2-14.1% $C_{l9:0}$ cyclo fatty acid was detected only in B. longum and the fatty acid was increased by the addition of the aeration. The $C_{l9:0}$ cyclic fatty acid was identified as a cis 9, 10-methylene octadecanoic acid, which was different from lactobacillic acid in the cyclized site. 6.6%-24.6% of dimethyl acetals (DMA) which came from plasmalogen were observed in the B. adolescentis and B. longum grown under anaerobic condition, and the components were notably decreased in the cells grown under the aerated conditions. It is believed that NADH oxidative enzymes play an important role to detoxify oxygen metabolites of Bifidobacteriurn spp. under anaerobic and microaerobic conditions. Independently from oxidative enzymes, it seems that oxygen stress may induce the change of the level of cellular fatty acids showing an increase of $C_{l9:0}$ cyclo in B. longum and a decrease of $C_{l8:1}$ of plasmalogen in B. longum and B. adolescentis to adapt in environment.

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Production of Bacillus anthracis Protective Antigen by Improvement of Culture Condition and Purification Methods (배양조건과 정제방법 개선을 통한 탄저균 방어항원의 생산)

  • 김성주;조기승;최영길;채영규
    • Korean Journal of Microbiology
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    • v.37 no.1
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    • pp.21-27
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    • 2001
  • Recently many investigators have devoted considerable attention to the production and purification of PA for antigens, and the preparation of new synthetic medium (RM medium) have solved to increase the yields of the PA but, the low sensitivity of the PA to detect B. anthracis infections has remained as a problem to be solved. This study was undertaken to evaluate the yields of the PA from culture filtrates of B. anthracis Sterne $34F_2$ strain in modified RM medium in which 10 g/l of $NaHCO_3$ and 10g/l of glucose were replaced by 8 g/l and 5 g/l, and the first purification step of PA from culture broth was used hydroxyapatite. The PA was purified by hydroxyapatite column chromatography, DEAE-Sepharose CL-4B column chromatography and Toyo-pearl gel filtration chromatography. The yield of PA from the modified RM medium, 8.6 mg/l.

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