• 제목/요약/키워드: L1

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다아체 형성에 의한 Philodendron Wend-imbe의 대량번식 (Micropropagation of Philodendron wend-imbe through Adventitious Multi-bud Cluster Formation)

  • 한봉희;예병우;구대회;유희주
    • Journal of Plant Biotechnology
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    • 제31권2호
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    • pp.115-119
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    • 2004
  • 본 실험은 Philodendron wend-imbe를 기내배양하여 일시에 균일한 식물체를 대량생산하기 위하여 실시하였다. Philodendron의 경정에서 다아체 형성은 BA 5.0-10.0mg/L 또는 TDZ 0.05-0.1 mg/L가 첨가된 MS 배지에서 양호하였다. 형성된 다아체 절편체 (5-7mm)를 BA 5.0 mg/L와 sucrose 20g/L가 첨가된 MS 배지에서 배양한 결과, 신초 및 다아체의 증식이 매우 양호하였으며, 신초기부에서 callus 발생이 억제되었다 다아체 절편체에서 신초의 발생 및 발근은 IBA 1.0-2.0mg/L 또는 NAA 0.1 mg/L가 첨가된 배지가 효과적이었으며, 발근된 신초의 순화는 perlite와 peat moss의 1:1 혼합용토 또는 peat moss가 적합하였다.

Screening of Antiviral Medicinal Plants against Avian Influenza Virus H1N1 for Food Safety

  • Lee, Jang-Hyun;Van, Nguyen Dinh; Ma, Jin-Yeul;Kim, Young-Bong;Kim, Soo-Ki;Paik, Hyun-Dong
    • 한국축산식품학회지
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    • 제30권2호
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    • pp.345-350
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    • 2010
  • Various extracts from 30 medicinal plants were evaluated for their antiviral activity against influenza virus A/Puerto Rico/8/34 (H1N1) and cytotoxicity in MDCK cell culture. The plant material (30 g) was extracted with methanol (300 mL) at room temperature for 24 h, after which the methanolic extracts were filtered, evaporated, and subsequently lyophilized. Evaluation of the potential antiviral activity was conducted by a viral replication inhibition test. Among these medicinal plants, Tussilago farfara, Brassica juncea, Prunus armeniaca, Astragalus membranaceus, Patrinia villosa, and Citrus unshiu showed marked antiviral activity against influenza virus A/H1N1 at concentrations ranging from 0.15625 mg/mL to 1.25 mg/mL, 0.3125 mg/mL to 10 mg/mL, 5 mg/mL to 10 mg/mL, 0.625 mg/mL to 10 mg/mL, 0.625 mg/mL to 10 mg/mL, and 0.3125 mg/mL to 5 mg/mL, respectively. The extracts of Tussilago farfara showed cytotoxicity at concentrations greater than 2.5 mg/mL, whereas the other five main extracts showed no cytotoxicity at concentrations of 10 mg/mL. Taken together, the present results indicated that methanolic extracts of the six main plants might be useful for the treatment of influenza virus H1N1.

Formation of Cadmium(II) Nitrate Complexes with Macrocycles

  • Ho-Doo Kim;Hak-Jin Jung;Oh-Jin Jung
    • Bulletin of the Korean Chemical Society
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    • 제14권5호
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    • pp.561-567
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    • 1993
  • The twelve macrocycle (L) complexes of cadmium(II) nitrate have been synthesized: $CdL(NO_3)_2$. All the complexes have been indentified by elemental analysis, electric conductivity measurements, IR and NMR spectroscopic techniques. The molar electric conductivities of the complexes in water and acetonitrile solvent were in the range of 236.8-296.1 $cm^2{\cdot}mol^{-1}{\cdot}ohm^{-1}$ at 25$^{\circ}$C. The characteristic peaks of macrocycles affected from Cd(II) were shifted to lower frequencies as compared with uncomplexed macrocycles. A complex with 1,4,8,11-tetrakis(methylacetato)-1,4,8,11-tetraaza cyclodecane (L4) exhibited two characteristic bands such as strong stretching (1646 $cm^{-1})$, and weaker symmetric stretching band (1384 $cm^{-1})$. NMR studies indicated that all nitrogen donor atoms of macrocycles have greater affinity to cadmium(II) metal ion than do the oxygen atoms. The $^{13}$C-resonance lines of methylene groups neighboring the donor atom such as N and S were shifted to a direction of high magnetic field and the order of chemical shifts were $L_1 < L_2 < L_3 < L_6 < L_4$. Also the chemical shifts values were larger than those of methylene groups bridgeheaded in side-armed groups. This result seems due to not only the strong interaction of Cd(Ⅱ) with nitrogen donors according to the HSAB theory, but weak interaction of Cd(Ⅱ) and COO- ions or sulfur which is enhanced by the flexible methylene spacing group in side-armed groups. Thus, each additional gem-methyl pairs of L_3, L_4\;and\; L_6$ macrocycles relative to $L_1, L_2,\;and\;L_5$ leads to an large enhancement in Cd(II) affinity. ^{13}C$-NMR spectrum of the complex with $L_{12}$ (1,5,9,13-tetracyclothiacyclohexadecane-3,11-diol) reveals the presence of two sets of three resonance lines, and intensities of the each resonance line have the ratio of 1 : 2 : 2. This molecular conformation is predicted as structure of tetragonal complex to be formed by coordinating two sulfur atoms and the other two sulfur atoms which is affected by OH-groups.

GPS L1/L2, GLONASS 수신기용 다중 대역 단일 패치 안테나 (A Single Layer Multi Band Microstrip Patch Antenna for GPS L1/L2, GLONASS Receiver Applications)

  • 김지혜;김미숙;김종성;손석보;김영백
    • 한국전자파학회논문지
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    • 제22권10호
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    • pp.990-998
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    • 2011
  • 본 논문에서는 GPS L2/L1, GLONASS 통합 수신기용 다중 대역 단일 패치 안테나를 설계 및 제작하였다. 설계된 안테나는 이중 급전 방식이고, 슬롯을 가진 단일 마이크로스트립 패치와 임피던스 매칭 회로로 구성되어 있다. 설계하고자 하는 안테나의 주요 목표 규격은 동작 주파수 대역인 GPS L2(1,227.6 MHz)/L1(1,575.42 MHz), GLONASS(1,602 MHz)에서 정재파비(VSWR: Voltage Standing Wave Ratio) 2.0 이하, 우선회 원형 편파(RHCP: Right-Hand Circularly Polarization) 특성, 30 dB 이상의 능동형 안테나 이득, 3 dB 이하의 축비이며, 시험을 통하여 주요 규격이 만족됨을 확인하였다.

경남 거창분지 정창리 뢰스-고토양 연속층의 퇴적물 특성과 편년 (Sedimentary Characteristics and Chronology of Loess-paleosol Sequence in Jeongjang-ri, Geochang basin, Gyeongnam Province)

  • 황상일;강창혁;윤순옥
    • 대한지리학회지
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    • 제46권1호
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    • pp.1-19
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    • 2011
  • 대자율 및 입도 분석, OSL 연대측정, 주원소, 희토류 및 미량원소 분석을 통해 거창 정장리 뢰스-고토양의 물리, 화학적 특징을 파악하였다. 거창 뢰스-고토양 연속층의 입도 특성은 하안단구를 형성하는 하천퇴적층 그리고 중국 뢰스고원과는 차이를 보인다. 뢰스-고토양 층서 가운데 L1과 L1L1은 MIS 2, L1S1은 MIS 3, L1L2는 MIS 4, S1은 MIS 5, L2는 MIS 6에 대비되므로 거창단면이 위치하는 하안단구는 MIS 7에 형성된 것으로 판단된다. 다만 MIS 3 시기에 남부지방이 중부지방보다 더 온난습윤하였을 가능성이 있다. 주원소와 희토류 원소, 미량원소 조성은 거창의 뢰스-고토양이 중국 뢰스고원보다 풍화가 더 많이 진행되었으며 중국 뢰스고원에서 기원하였음을 지시한다. 이것은 한반도의 다른 지역에서 이루어진 연구 결과와 조화된다.

Effects of Elicitors on Seedling Growth, Total Polyphenol and Chlorophyll Content and Antioxidant Activity of Barley (Hordeum vulgare L.)

  • Salitxay, Timnoy;Phouthaxay, Phonesavanh;Pang, Yeoun-Gyu;Yeong, Yu-Chi;Adhikari, Prakash Babu;Park, Cheol-Ho
    • 한국작물학회지
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    • 제61권1호
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    • pp.33-40
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    • 2016
  • This study is focused on the evaluation of growth parameters, total polyphenol content (TPC), chlorophyll content as well as the DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging activity of young barley seedling (YBS) affected by elicitation. Salicylic acid (SA), methyl jasmonate (MJ), amino acid liquid fertilizer (ALF) and microbial metabolism activator (MMA) were used. Elicitation was conducted for two times and various concentrations were used in this study. The result revealed that, MJ 1 ml/L treated-YBS gave the longest seedling length of 1.33 cm, followed by the ones treated with SA 1.38 mg/L and ALF 2 ml/L, respectively. ALF 3 ml/L treatment gave the highest fresh weight of 10 seedlings, followed by MJ 5 ml/L and SA 13.8 mg/L treatment with 1.56 g, 1.55 g and 1.53 g respectively. SA 138.12 mg/L elicitor treated-YBS gave the highest Chl a, Chl b content of $8.57{\mu}g/mg$ and $3.83{\mu}g/mg$, respectively while the highest carotenoid content was found in MJ ml/L treatment with $1.62{\mu}g/mg$. Among elicitor treated-YBS, SA showed better TPC. The highest TPC was found in SA 1.38 mg/L treatment with 18.82 mg/g TAE. Likewise, SA 1.38 mg/L showed the highest DPPH free radical scavenging activity among all the treatments. However, the lowest TPC was found in ALF 1ml/L treated-YBS with 9.46 mg/g TAE, which was even lower than the control (14.31 mg/g TAE).

수직 등온 평행 평판에서 상호작용 유동이 대류 열전달에 미치는 영향 (The Effect of the Interactive Flow on Convective Heat Transfer from two Vertical Isothermal Parallel Plates)

  • 김상영;정한식;권순석
    • 대한기계학회논문집
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    • 제16권4호
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    • pp.765-774
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    • 1992
  • 본 연구에서는 수직 등온 평행평판의 혼합대류 열전달에 대하여 두 평판의 길 이가 다른 경우를 고찰하고자 Reynolds수, Grashof수, 평판간격을 변수로 하여 수치해 석 하였다.

Zymomonas mobilis와 Gluconobacter suboxydans를 이용한 돼지감자로부터 D-sorbitol 및 L-sorbose 생성에 관한 연구 (Production of D-sorbitol and L-sorbose from Jerusalem artichoke by Zymomonas mobilis and Gluconobacter sMboxpydans)

  • 전억한;김원극
    • KSBB Journal
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    • 제8권1호
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    • pp.10-16
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    • 1993
  • The use of Jerusalem artichoke containing $\beta$-1, 2-fructose oligomer for the production of D-sorbitol and L-sorbose has been studied. The employment of inulinase(0.398%, v/v) for the hydrolysis of 40% (v/w) Jerusalem artichoke juice resulted in 36.7g/1 of glucose and 85.3g/1 of fructose at $50^{\circ}C$. These sugars were utilized as substrates for D-sorbitol and L-sorbose production. Coimmobilization of inulinase and permeabilized cells of Zymomonas mobilis in the mixture of chitin (5%, w/e) and x-carrageenan(4%, w/v) resulted in the production of 30.2g/1 of D-sorbitol by using inulin as a substrate. The process of L-sorbose production from D-sorbitol by Gluconobacter suboxydans was optimized with respect to the substrate concentration, level of dissolved oxygen and glucosic and concentration. Gluconlc acid produced by Zymomonas mobilis from glucose was found to inhibit Gluconobacter suboxtans in conversion of D-sorbitol to L-sorbose. In view of removing such inhibitory effect by gluconic acid, mutants were selected by the NTG (N-methyl-N'-N'-nitro-N-nitrosoguanidlne) treated method. Mutants selected by NTG mutagenesis showed no inhibitory effects of gluconic acrid against L-sorbone production when its concentration increased up to 100g/1. A mutant produced 40.1g/l of L-sorbose in the medium containing 100g/l D-sorbitol and 100g/l-gluconic acid. This result is consider able when compared with L-sorbose concentration (21.7g/1) obtained from the fermentation with wild type strain of Gluconobacter suboxnians.

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파 지하부로부터 분리된 2종의 Diosgenin 배당체의 구조 (The Structures of Two Diosgenin Glycosides Isolated from the Subterranean Parts of Allium fistuiosum)

  • Jung, Keun-Young;Do, Jae-Chul;Son, Kun-Ho
    • 한국식품영양과학회지
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    • 제22권3호
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    • pp.313-316
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    • 1993
  • 파 지하부로부터 분리된 2종의 diosgenin 배당체의 구조를 그의 분광학적 data와 화학적 실험 등의 방법으로 결정하였다. 그 결과, 이 두화합물의 구조는 diosgenin 3-O-$\alpha$-L-rhamnopyranosyl (1$\longrightarrow$2)-[$\alpha$-L-rhamnopyranosyl (1$\longrightarrow$4)]-$\beta$-D-glucopyranoside (1, dioscin)과 diosgenin 3-O-$\alpha$-L-rhamnopyranosyl (1$\longrightarrow$4)-$\alpha$-L-rhamnopyranosyl (1$\longrightarrow$4)]- [$\alpha$-L-rhamnopyranosyl (1$\longrightarrow$2]-$\beta$-D-glucopyranoside (2, saponin P-d)로서 기지 물질이나, saponin P-d를 부분가수분해하여 얻은 prosapogenin 중 compound 4는 문헌 미기재의 물질이다.

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Reactivity of RhCp* Complexes Containing Labile Ligands toward Potential Linking Ligands Containing Terminal Thiophene or Furan Rings:Preparation and Structures of [Cp*Rh(L1)Cl2], [Cp*Rh(η2-NO3)(L1)](OTf),and {[Rh(L2)] · (OTf)}[L1 = 1,2-Bis((thiophen-2-yl)methylene)hydrazine); L2 = 1,2-Bis((furan-2-yl)methylene)hydrazine]

  • Lee, Kyung-Eun;Lee, Soon-W.
    • Bulletin of the Korean Chemical Society
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    • 제31권12호
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    • pp.3600-3604
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    • 2010
  • Rhodium(III)-$Cp^*$ complexes containing labile ligands, $[Cp^*RhCl_2]_2$, [$Cp^*Rh({\eta}^2-NO_3)$(OTf)], and $[Cp^*Rh(OH_2)_3](OTf)_2$, reacted with potential linking ligands [$L^1$ = (2-thiophene)-CH=N-N=CH-(2-thiophene); $L^2$ = (2-furan)-CH=N-N=CH-(2-furan)] to give two molecular compounds, [$Cp^*Rh(L^1)Cl_2$] (1) and [$Cp^*Rh({\eta}^2-NO_3)(L^1)$]$(OTf){\cdot}CH_2Cl_2$ ($2{\cdot}CH_2Cl_2$), and one 1-dimensioanl coordination polymer, $\{[Rh(L^2)]{\cdot}(OTf)}_{\infty}$ (3). Whereas one imine nitrogen atom within the ligand is coordinated to the Rh metal in compounds 1 and 2, both nitrogen atoms are bound to two neighboring Rh metals in compound 3 to lead to a 1-D chain polymer.