• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.522-529
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• 1998

This experiment was conducted to study the effects of lactic acid bacteria (LAB) inoculation either alone or in combination with cell wall degrading enzymes on the fermentation characteristics and chemical compositions of Rhodesgrass silage. Over to 1 kg of fresh Rhodesgrass sample a treatment of inoculant LAB with or without addition of an enzyme of Acremoniumcellulase (A) or Meicelase (M) or a mixture of both enzymes (AM) was applied. The treatments were control untreated, LAB-treated (application rate $1.0{\times}10^5cfu/g$ fresh sample), LAB+A 0.005%, LAB+A 0.01%, LAB+A 0.02%, LAB+M 0.005%, LAB+M 0.01%, LAB+M 0.02 %, LAB+AM 0.005%, LAB+AM 0.01%, and LAB+AM 0.02%. The sample was ensiled into 2-L vinyl bottle silo, with 9 silages of each treatment were made. Three silages of each treatment were incubated at 20, 30 and $40^{\circ}C$ for 2-months of storage period. All silages were well preserved with their fermentation quality has low pH values (3.91-4.26) and high lactic acid concentrations (4.11-9.89 %DM). No differences were found in fermentation quality and chemical composition of the control untreated silage as compared to the LAB-treated silage. Combined treatment of LAB+cellulases improved the fermentation quality of silages measured in terms of lower (p < 0.01) pH values and higher (p < 0.05) lactic concentrations than those of LAB-treated silages. Increasing amount of cellulase addition resulted in decrease (p < 0.05) of pH value and increase (p < 0.05) of lactic acid concentration. LAB + cellulase treatments (all cellulase types) reduced (p < 0.01) NDF, ADF and in vitro dry matter digestibility of silages compared with the control untreated silages. The fermentation quality and the rate of cell wall reduction were higher (p < 0.01) in the silages treated with LAB + cellulase A than in the silages treated with either LAB+cellulase M or LAB + cellulase AM. Incubation temperature of $40^{\circ}C$ was likely to be more appropriate environment for stimulating the fermentation of Rhodesgrass silages than those of 20 and $30^{\circ}C$.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8253-8258
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• 2016

Elevated levels of soluble CD30 (sCD30) are linked with various T-cell neoplasms. However, the relationship between sCD30 levels and the development of adult T-cell leukemia (ATL) in human T-cell leukemia virus type 1 (HTLV-1) carriers remains to be clarified. We here investigated whether plasma sCD30 is associated with risk of ATL in a nested case-control study within a cohort of HTLV-1 carriers. We compared sCD30 levels between 11 cases (i.e., HTLV-1 carriers who later progressed to ATL) and 22 age-, sex- and institution-matched control HTLV-1 carriers (i.e., those with no progression). The sCD30 concentration at baseline was significantly higher in cases than in controls (median 65.8, range 27.2-134.5 U/mL vs. median 22.2, range 8.4-63.1 U/mL, P=0.001). In the univariate logistic regression analysis, a higher sCD30 (${\geq}30.2U/mL$) was significantly associated with ATL development (odds ratio 7.88 and the 95% confidence intervals 1.35-45.8, P = 0.02). Among cases, sCD30 concentration tended to increase at the time of diagnosis of aggressive-type ATL, but the concentration was stable in those developing the smoldering-type. This suggests that sCD30 may serve as a predictive marker for the onset of aggressive-type ATL in HTLV-1 carriers.

• 한국식품영양학회지
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• 제8권4호
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• pp.275-279
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• 1995

A bacterium L-10 which produce mush of glutamic acid was Isolated from soil and identified as the genus Pserdomonas. The maximal glutamic acid production was obtained when the strain was cultured at 3$0^{\circ}C$ for 30 hrs in the optimal medium containing 5% glucose, 0.5% each of urea and yeast extract, 0.1% K2HP04, 0.02% MgSO4.7H20, 0.3% (NH, )rHP04, 0.5ug/l biotin and Initial pH 7.0, and then final glutamic acid production under the above conditions was 1.2mg/ml of cell cultures.

• 한국식품저장유통학회지
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• 제19권1호
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• pp.138-143
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• 2012

늙은 호박의 물 및 70% 에탄올 추출물에 대한 세포의 독성 및 지방생성에 미치는 영향을 조사하였다. 세포 생존율 측정에서 늙은 호박 추출물이 0.5-5 mg/mL의 농도일 때 물 및 에탄올 추출물 모두 세포의 생장에 영향을 미치지 않는 것으로 나타나 세포에 대한 독성이 없다고 판단되었다. 3T3-L1 지방세포의 지방축적에 미치는 영향을 1 mg/mL 농도로 조사하였을 때 에탄올 추출물은 지방축적을 저해 하였으나 물 추출물은 저해력이 없는 것으로 나타났다. 따라서 0.5-5 mg/mL로 농도를 달리하여 조사한 결과 두 추출물 모두 농도 의존적으로 지방축적을 저해하는 것으로 나타났으며 물 추출물과 에탄올 추출물 농도가 5 mg/mL 일 때 각가 85.22% 75.35%의 지방축적을 보였다. 따라서 본 실험에서는 늙은 호박의 물 및 70% 에탄올 추출물이 3T3-L1 지방세포의 분화를 억제하였으나 다른 많은 천연물에 비해 다소 높은 농도에서 효과가 나타났다. 또한 총 폴리페놀 함량조사 결과, 물 추출물에서 최대 $46.54{\pm}0.02$ mg을 함유한 것으로 나타나 늙은 호박은 제약 원료로는 제한점이 있으나 식품으로 꾸준히 섭취 할 경우 항비만 소재로서의 가능성이 있을 것으로 생각된다.

• 한국식물병리학회지
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• 제3권2호
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• pp.124-130
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• 1987

수도 도열병균(Pyricularia oryzae)의 균사체로부터 다량의 원형질체의 분리 및 세포벽 재생을 하기 위하여 필요한 몇가지 조건을 선발하였다. 나출을 위하여 기본용액으로 0.02M potassium phosphate buffer와 pH 5.2, 0.6M KCl로 삼투압을 조절하였고 분해효소는 ml당 각각 20mg Cellulase R-10, 5mg Macerozyme $-10, 10mg Driselase를 사용하였는데 각각의 단독처리구보다 3가지 효소의 복합처리구에서 원형질체 나출 정도가 우수하였다. 또한 선발된 복합효소액에 2일간 액체배양된 어린 균사체를 3시간, $30^{\circ}C$ 항온기에서 진탕했을 때 가장 많은 원형질체가 분리되었다. 원형질체로부터 세포벽의 재생은 순수 정제한 원형질체를 0.2M potassium phosphate와 0.6M KCl을 삼투압을 조절한 감자한철배지에 접종시켜 가장 높은 재생율을 얻을 수 있었다.

• 한국식품위생안전성학회지
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• 제38권6호
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• pp.544-550
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• 2023

치주질환의 원인균인 Porphyromonas gingivlais (P. gingivalis)의 병인인자중 하나인 gingipain 효소에 대한 억제활성을 갖는 유산균을 개발하고자 본 연구실에 보관중인 30여종의 유산균 배양상등액의 활성을 검색하였다. 그 결과 발효식품에서 분리, 동정한 Weisiella cibaria SPM402와 Lactobacillus paracasei SMP412가 P. gingivalis에 대한 항균효과, gingipain 유전자 발현억제 및 gingipain 효소활성 억제능이 확인되었다. W. cibaria SPM402 의 배양 상등액 WC402는 10 mg/mL에서 kgp 유전자는 0.71±0.02배 발현되었고, rgpB 유전자는 0.71±0.14배 발현되 약간의 발현억제 효과가 확인되었다. 반면 L. paracasei SMP412의 배양상등액 LP412는 10 mg/mL 농도에서 kgp 유전자는 0.19±0.08배, rgpA 유전자는 0.09±0.02배, rgpB 유전자는 0.24±0.03배 발현되어 LP412의 뛰어난 gingipain 유전자 발현억제 효과를 확인하였다. 10 mg/mL농도에서, Kgp 효소 활성은 WC 402에 의해 78.65±3.58% (CAG), 82.45±1.22% (CFG), LP412에 의해 80.71±2.11% (CAG), 85.81±0.05% (CFG) 억제되었다. 동일한 농도에서 Rgp 활성은 WC402에 의해 78.6±1.01% (CAG), 86.78±0.47% (CFG), LP412에 의해 82.93 ±1.26% (CAG), 88.81±0.36% (CFG) 억제되었다. 이상의 결과들을 통해 WC402와 LP412는 P. gingivalis에 대한 항균효과 뿐 아니라 병인인자 gingipain을 효과적으로 억제하는 기능성 유산균임을 확인하였다.

• 한국식품영양과학회지
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• 제25권4호
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• pp.708-714
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• 1996

호기성의 Rhizopus japonicus와 혐기성 Zymomonas mobilis로 구성된 혼합고정화 배양계(R-Z계)를 제조하고, 생전분으로부터 에탄올 생산에 응용하였다. R. japonicus를 고정화배양하므로서 액체배양에서 보다 2배 높은 glucose량을 얻었다. R-Z계의 에탄올 생산량은 1.67g/L(Yp/s, 0.094)이 었지만, 배양 24시간째부터 산소공급을 억제한 R-Z 24계에서는 6.54g/L(Yp/s, 0.38)의 에탄올을 얻어 대조구의 약 4배를 향상시켰다. 회분배양에서는 5%의 기질 농도가 적당하였으며, 생산된 에탄올은 15.02g/L(Yp/s, 0.36)이었다. 2% 기질을 5회 첨가한 유가배양에서는 2%기질의 회분배양과 동등한 수율인 0.38을 얻었다.

• 상하수도학회지
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• 제30권4호
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• pp.381-389
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• 2016

Jeju Eoseungsaeng lake which is a main water resource of Eoseungsaeng water treatment plant($Q=15,000m^3/d$) experienced high chromaticity(40 CU) and pH(9.46) in 2013. This could decline customer's confidence on drinking water quality unless proper identification and removal of chromaticity were implemented. To find cause of chromaticity, water monitoring on various water parameters including TOC, algal cell count, Chl-a, turbidity, SS, conductivity, etc. were implemented. Iron and manganese were excluded from the cause of chromaticity due to its low concentration (i.e., < 0.02 mg/L). Correlation among water parameters showed that relationship between algal cell count and chromaticity was the highest(R=0.43), which suggested that presumably the main reason of chromaticity occurrence in Jeju Eoseungsaeng lake was algae.

• Archives of Pharmacal Research
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• 제27권4호
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• pp.436-441
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• 2004

SJ compounds (SJ8002 and related compounds) are a group of novel anticancer agents (Cho, Chung, Lee, Kwon, Kang, Joo, and Oh. PCT/KR02/00392). To explore the anticancer mechanism of these compounds, we examined the effect of SJ8002 on microtubules of six human cell lines. At a high concentration ($2\;{\mu}g/mL$), SJ8002 effectively disrupted microtubules of the six cell lines within 1 h. At lower concentrations ($0.05\~1.0\;{\mu}g/mL$), the antimicrotubule activity of SJ8002 varied defending on cell lines. The inhibition of in vitro polymerization of pure tubulin by SJ8002 suggested that SJ8002 acts on free tubulin, inhibits the polymerization of tubulin dimer into microtubules, and hence induces the depolymerization of microtubules.

• Plant Resources
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• 제7권1호
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• pp.1-6
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• 2004

In vitro proliferation system was achieved by using nodal segment excised from greenhouse grown juvenile stock plants of Alnus japonica. Stem explants were cultured on MS medium supplemented with different plant growth regulators of cytokinin and/or their combinations. The most effective cytokinin source was the combination of zeatin 2.0 mg/L and TDZ 0.05 mg/L producing the average number of shoots (16.8 $\pm$ 3.6). In addition, healthy roots were formed after small clumps of shoots were transferred to half strength of MS medium containing IBA 0.02 mg/L with optimal rooting capacity. Soil acclimatization was successfully conducted in cell tray containing artificially mixed soil with 92 % survival rate.