Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Microtubule Inhibitory Effects of Various SJ Compounds on Tissue Culture Cells

  • Lee Jong Han (Department of Biology and the Institute of Life Sciences and Biotechnology Yonsei University) ;
  • Kang Dong Wook (Central Research institute, Samjin Pharm. Co., Ltd.) ;
  • Kwon Ho Suk (Central Research institute, Samjin Pharm. Co., Ltd.) ;
  • Lee Sun Hwan (Central Research institute, Samjin Pharm. Co., Ltd.) ;
  • Park Si Kyung (Central Research institute, Samjin Pharm. Co., Ltd.) ;
  • Chung Sun Gan (Central Research institute, Samjin Pharm. Co., Ltd.) ;
  • Chon Eui Hwan (Central Research institute, Samjin Pharm. Co., Ltd.) ;
  • Paik Soon Young (Department of Microbiology The Catholic University) ;
  • Lee Joo Hun (Department of Biology and the Institute of Life Sciences and Biotechnology Yonsei University)
  • Published : 2004.04.01
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Abstract

SJ compounds (SJ8002 and related compounds) are a group of novel anticancer agents (Cho, Chung, Lee, Kwon, Kang, Joo, and Oh. PCT/KR02/00392). To explore the anticancer mechanism of these compounds, we examined the effect of SJ8002 on microtubules of six human cell lines. At a high concentration ($2\;{\mu}g/mL$), SJ8002 effectively disrupted microtubules of the six cell lines within 1 h. At lower concentrations ($0.05\~1.0\;{\mu}g/mL$), the antimicrotubule activity of SJ8002 varied defending on cell lines. The inhibition of in vitro polymerization of pure tubulin by SJ8002 suggested that SJ8002 acts on free tubulin, inhibits the polymerization of tubulin dimer into microtubules, and hence induces the depolymerization of microtubules.

Keywords

References

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