• Title/Summary/Keyword: L-glutamic acid

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Properties of Kimchi Fermented with GABA-Producing Lactic Acid Bacteria as a Starter

  • Lee, Kang Wook;Shim, Jae Min;Yao, Zhuang;Kim, Jeong A;Kim, Jeong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.28 no.4
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    • pp.534-541
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    • 2018
  • Kimchi (a traditional Korean fermented vegetable) was prepared with a starter, Lactobacillus zymae GU240 producing ${\gamma}$-aminobutyric acid (GABA), and one precursor of GABA (glutamic acid, glutamic acid monosodium salt (MSG), or kelp extract). L. zymae GU240, an isolate from kimchi, can grow at 7% NaCl and low temperature. Five different kimchi samples were fermented for 20 weeks at $-1^{\circ}C$. Kimchi with starter alone could not produce GABA. The GABA content was highest in kimchi with co-inoculation of the starter and MSG (1% (w/w)). Kimchi co-inoculated with the starter and kelp extract powder (3% (w/w)) had the second highest GABA content. Addition of glutamic acid powder (1% (w/w)) caused a reduction in the pH level of kimchi and growth inhibition of lactic acid bacteria and yeasts. Kimchi samples with MSG or kelp extract showed improvement of sensory evaluation scores. The results demonstrate the possibility to produce kimchi with improved functionality and taste by using L. zymae GU240 as a starter along with a suitable precursor such as MSG or kelp extract.

Preparation of Onion Hydrolysates with Enzyme (효소에 의한 양파가수분해액의 제조)

  • 서형주;정수현;손종연;손흥수;조원대;마상조
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.5
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    • pp.786-790
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    • 1996
  • The changes in chemical constituents of onion in Korea and the degree of hydrolysis in onion by-drolysates by treatment of viscozyme L, celluclast 1.5L, pectinex and cereflo were investigated. Proximate compositions of onion in Korea were as follows; The contents of moisture, protein, lipid, carbohydrate and ash were 89~90%, 1.8~1.9%, 0.4~0.5%, 7.1~8.5% and 0.3~0.5%, respectively. Major free sugars were identified to be glucose, fructose and sucrose and major free amino acids were as cysteine, histidine, arginine and glutamic acid. The degree of hydrolysis by treatment of viscozyme L, celluclast 1.5L, pectinex and cereflo was 69%, 71%, 80% and 62%, respectively. During the enzymatic hydrolysis, free sugar and total sugar content increased however, pyruvic acid content decreased significantly.

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On the Production of L-Glautamic Acid from Acetate by Some Microbes (빙초산을 탄소원으로 한 글루타민산 생성에 관한 연구)

  • 유영진;박계인;김기주;한덕봉;김요성
    • Korean Journal of Microbiology
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    • v.11 no.2
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    • pp.59-62
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    • 1973
  • Bacterium strain, K-173-10, which was isolated from waste soil of Korean brewing factories, could grow on acetate as the sole carbone source and accumulate a considerable amount of L-glutamic acid (24g/l) in the liguid culture medium. This strain was named by Brevibacterium ammoniagenes sp. by the standard method of taxonomy procedures given in the Manual of Microbiogical Methods.

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Comparison of the Chemical Components of Buckwheat Seed and Sprout (메밀종자와 메밀나물의 화학적 성분비교)

  • Kim, Youn-Sun;Kim, Jong-Goon;Lee, Young-Sook;Kang, Il-Jun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.1
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    • pp.81-86
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    • 2005
  • The chemical components of buckwheat seed and sprout were compared for predicting the usefulness of buckwheat sprout as food materials. The buckwheat sprout was harvested and lyophilized after germination for 7 days. Crude protein, lipid and ash contents of buckwheat sprout were 20.8, 1.3 and 2.6% in dry basis, respectively. Major amino acids of buckwheat sprout were glutamic acid (2,764 mg/l00 g) and aspartic acid (1,698 mg/l00 g). The contents of tryptophan, alanine, tyrosine and histidine of buckwheat sprout were about 1.7 to 1.9 times higher than those of buckwheat seed. Major fatty acids of buckwheat sprout were linoleic acid (45.9%) and oleic acid (18.4%). The contents of stearic acid (18:0) and oleic acid (18:1) were decreased by about 21% and 50%, whereas those of linoleic acid (18:2) and linolenic acid (18:3) were increased by 1.3 and 5.4 times, respectively after germination for 7 days. The mineral contents of buckwheat sprout were 152.0 mg/l00 g for Ca, 9.9 mg/l00 g for Zn, 485.0 mg/l00 g for Mg and 5.4 mg/l00 g for Fe. Vitamin A, C and E contents of buckwheat sprout were 1,180 IU/100 g, 203 mg/l00 g and 32.1 mg/l00 g in dry basis, respectively. Especially, the content of $\alpha$ -tocopherol was increased by 27.5 times as compared to that of buckwheat seed. The rutin content of buckwheat sprout was 343.67 mg/l00 g, which was about 18 times higher than that of buckwheat seed.

Effects of Ultrasonic Treatment Time on the Quality and Palatability of fried Chicken Meat (초음파 처리시간이 튀김 계육의 품질 및 기호성에 미치는 영향)

  • 남주현;송형익;박충균;박성하;김도완;문윤희;정인철
    • Food Science of Animal Resources
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    • v.22 no.2
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    • pp.115-121
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    • 2002
  • This study was carried out to investigate the effect of ultrasonic treatmeat time on the quality and palatability of fried chicken meat. Moisture content, U-10 treatment (chicken meat treated by ultrasonification for 10 minutes) of breast and leg were lower than those of the control, protein contents were not different between samples. Fat content was higher with increasing ultrasonic treated time. Frying loss of ultrasonic treated breast and leg were lower than those of the control, water holding capacity of ultrasonic treated breast and leg were higher than those of the control with increasing ultrasonic treated time. Rheological textures between control and ultrasonic treated samples were not different. L* value(lightness) between control and ultrasonic treated samples were not different, but L* values of breast were higher than those of the leg. And a* value(redness) was not effect by ultrasonic treatment, but a* value of leg were higher than those of the breast. Amino acid composition has included many glutamic acid, aspartic acid, lysine, leucine and arginine. Oleic acid and linoleic acid occupied beyond 50% of fatty acid composition. And taste, texture, juiciness and palatability improved with increasing ultrasonic treated time.

Physicochemical Properties of the Mung Bean (Phaseolus aureus L.) as Biohealth Functional Substance (기능성 소재로서 녹두(Phaseolus aureus L.)의 이화학적 특성)

  • Lee, Young-Geun;Bark, Si-Woo;Kim, Han-Soo
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.4
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    • pp.1096-1107
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    • 2019
  • This study on the physicochemical property of physiological activity substance in mung bean (Phaseolus aureus L.) was performed for the use as an functional food materialization. The proximate composition in the vacuum freeze dried mung bean was carbohydrate 57.20±0.29%, crude protein 26.40±0.69%, moisture 9.90±0.16%, crude ash 3.54±0.43%, and crude fat 2.96±0.26%, respectively. The vitamin content of mung bean was vitamin B5 0.62±0.013 mg/100 g, vitamin E 0.17±0.001 mg/100 g, vitamin B1 0.13±0.016 mg/100 g, and β-carotene 87.37±0.754 ㎍ RE/100 g, respectively. The mineral content of mung bean was potassium (K) 12,428.55±147.55 mg kg-1, magnesium (Mg) 2,053.32±14.13 mg kg-1, calcium (Ca) 1,966.40±14.53 mg kg-1, sodium (Na) 1,063.99±7.75 mg kg-1, iron (Fe) 63.77±0.98 mg kg-1, and manganese (Mn) 14.67±0.22 mg kg-1. The compositions of fatty acid were saturated fatty acid 29.23±0.03%, monoenes 20.30±0.04%, and polyenes 50.46±0.06%. Protein bound amino acid content of mung bean was 21.75±0.24 g%. And major amino acids were glutamic acid 3.93±0.03 g%, aspartic acid 2.68±0.03 g%, respectively. The composition of free amino acid of mung bean was 336.77±8.66 mg%, and major free amino acids were arginine, glutamic acid, asparagine, and aspartic acid. As a results of these experiment, Mung bean could be used a natural resouce and functional biohealth food substance.

Analysis of Community Level Physiological Profiles in the Rhizosphere of Brassica rapa subsp. pekinensis (Brassica rapa subsp. pekinensis 근권 서식 미생물의 기질이용 활성 조사)

  • Jung, Se-Ra;Kim, Seung-Bum
    • Korean Journal of Environmental Biology
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    • v.26 no.1
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    • pp.42-46
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    • 2008
  • The community size of culturable heterotrophic bacteria and community level physiological profiles (CLPP) in the rhizosphere of Brassica rapa subsp. pekinensis (Chinese cabbage) were analyzed in two different sites. The average community size of culturable heterotrophic bacteria ranged between $2.65\times10^6CFU\;g^{-1}$ soil (Suwon) and $3.75\times10^6CFU\;g^{-1}$ soil (Yesan), whereas those of bulk soils ranged between $2.45\times10^6CFU\;g^{-1}$ soil (Suwon) and $2.97\times10^6CFU\;g^{-1}$ soil (Yesan). The average functional richness of Suwon rhizoshpere was 90.8, whereas that of Yesan rhizosphere was 154.1. High level of correlation was found between the community size and functional richness. The most actively utilized substrates in both rhizospheres were adonitol, L-asparagine, D-gluconic acid, L-glutamic acid and D-galacturonic acid. Clear differences were seen in the utilization patterns between the two sites. Differences were also observed for the patterns of bulk soils between the two sites, although D-raffinose and D-mannose were found as the commonly utilized substrates.

Chemical Compounds and Biological Activity of Phellinus baumii (상황버섯의 화학성분 및 생리활성 효과)

  • Shon, Mi-Yae;Seo, Kwon-Il;Choi, Sun-Young;Sung, Nak-Ju;Lee, Sang-Won;Park, Seok-Kyu
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.5
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    • pp.524-529
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    • 2006
  • Chemical compounds, hydrogen peroxide and nitrite-scavenging activities of Phellinus baumii (PB) were investigated to expand the utilization of PB as functional food material. Total mineral contents of PB was 534.3 mg% and potassium was the highest content being 224 mg%. Total and reducing sugars were 56.2% and 9.8%, respectively The contents of free amino acids (FAAs) were in a range of $16.9{\sim}765.5mg%$ with the major FAAs of phenylalanine, aspartic acid, glutamic acid, leucine, serine and valine. The contents of total phenolic compounds in methanol and hot water extracts of PB were 33.3 and 20.7 mg/100 mL, respectively and were higher than those of other solvent extracts. Hydrogen peroxide-scavenging activity (80%) of methanol extract at $10{\mu}g/mL$ for 30 min was similar to tocopherol (83.1%) as control. Nitrite-scavenging activity of extracts of methanol and hot water at 500 mg/mL and pH 1.2 were 57.3% and 51.8%, respectively and then their effects were increased by lowering pH. The present results showed that the methanol and water extracts of Phellinus baumii exhibited strong hydrogen peroxide and nitrite-scavenging activities.

Effects of Molecular Weights on the Physico-pharmaceutical Properties of Poly-L-glutamic acid-cytarabine Conjugates

  • Kim, Chong-Kook;Kwon, Kyoung-Ae;Jeong, Eun-Ju;Lee, Myung-Gull
    • Archives of Pharmacal Research
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    • v.12 no.2
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    • pp.88-93
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    • 1989
  • In order to obtain some informations about the effect of molecular weight on the release rate of drug from drug carrier, two types of poly-L-glutamic acid (PLGA)-cytarabine (ara-C) conjugates, PLGA-ara-C:I and PLGA-ara-C:II, were synthesized using two types of PLGA having different average molecular weight, 43,000 and 77,800, respectively. The PLGA-ara-C conjugates were synthesized by mixed anhydride method and found to be covalently linked. Both types of conjugates charged negatively at biological pH. The pH-dependent release rate of ara-C was observed in both cases, and the release rate was accelerated in basic, acidic conditions (the k values were 0.015 $day^{-1}$ at pH 7.0, 0.024 $day^{-1}$ at pH 5.0, and 0.059 $day^{-1}$ at pH 9.0 in the case of PLGA-ara-C:I) and in the presence of pretense. The time required for the release of 16.5% of ara-C from PLGA-ara-C:I were 8 hr and 144 hr in the presence and absence of protease, respectively. Although both types of conjugates showed similar drug substitution ratio, they showed different release rates. Between the two types of conjugates, PLGA-ara-C:II showed the faster release rate (0.030 vs 0.042 $day^{-1}$ in pH 7.4 phosphate buffer solution at $37^{\circ}C$) and the smaller activation energy for the release of drug (12.5 vs 7.7 Kcal/mol) than PLGA-ara-C:I. The characteristic effect of molecular weight on the release rates of PLGA-ara-C conjugates suggests that the drug release rate might be effectively controlled over a prolonged period of time by the combined use of the different types of PLGA-ara-C conjugates having different molecular weights.

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Quality Characteristics of Soybean Curd Mixed with Freeze Dried Onion Powder (동결건조 양파분말을 첨가한 두부의 품질특성)

  • Kang, Nan-Suk;Kim, Jun-Han;Kim, Jong-Kuk
    • Food Science and Preservation
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    • v.14 no.1
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    • pp.47-53
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    • 2007
  • Soybean curd was mixed with onion powder to develop new foods, and changes in quality characteristics were investigated. The moisture content of onion soybean curd rose as the proportion of onion powder increased Whiteness (as measured by the L value) was high in soybean cud admixed with 0.1% (w/v) onion powder, Redness (the a value) was not significantly altered (the readings were 1.03-1.54) on addition of various onion powder concentrations. Yellowness (the b value) was similarly unaffected (readings 13.00-13.93) when various levels of onion powderwere added. Free sugar analysis showed that glucose was high in soybean curd (67.22 g/100 g) admixed with 0.1% (w/v) onion powder, The main organic acid was tartaric acid, and control organic acids included citric and oxalic acids at high levels. The major free amino acids were L-arginine, ${\gamma}-Amino-n-butyric$ acid, L-histidine, L-glutamic acid, L-serine, L-tyrosine and L-threonine, and amino acid content were high in soybean curd admixed with 0.2% (w/v) onion powder. Major phenolic compounds of onion soybean curd were quercitrin, protocatechuic acid and caffeic acid. The hardness of onion soybean curd was similar to that of the control when onion powder was added to 0.1% or 0.2% (w/v), and decreasedmore onion powder was added. Organoleptic qualities dropped as onion powder levels increased. In summary, onion powder addition to soybean curd is optimal at the 0.2% (w/v) level..