Analysis of Community Level Physiological Profiles in the Rhizosphere of Brassica rapa subsp. pekinensis

Brassica rapa subsp. pekinensis 근권 서식 미생물의 기질이용 활성 조사

  • Jung, Se-Ra (Graduate School of Public Health and Biotechnology, Chungnam National University) ;
  • Kim, Seung-Bum (Department of Biological Sciences, Chungnam National University)
  • 정세라 (충남대학교 보건바이오산업대학원 보건바이오산업학과) ;
  • 김승범 (충남대학교 생명과학부 생명과학과)
  • Published : 2008.03.30

Abstract

The community size of culturable heterotrophic bacteria and community level physiological profiles (CLPP) in the rhizosphere of Brassica rapa subsp. pekinensis (Chinese cabbage) were analyzed in two different sites. The average community size of culturable heterotrophic bacteria ranged between $2.65\times10^6CFU\;g^{-1}$ soil (Suwon) and $3.75\times10^6CFU\;g^{-1}$ soil (Yesan), whereas those of bulk soils ranged between $2.45\times10^6CFU\;g^{-1}$ soil (Suwon) and $2.97\times10^6CFU\;g^{-1}$ soil (Yesan). The average functional richness of Suwon rhizoshpere was 90.8, whereas that of Yesan rhizosphere was 154.1. High level of correlation was found between the community size and functional richness. The most actively utilized substrates in both rhizospheres were adonitol, L-asparagine, D-gluconic acid, L-glutamic acid and D-galacturonic acid. Clear differences were seen in the utilization patterns between the two sites. Differences were also observed for the patterns of bulk soils between the two sites, although D-raffinose and D-mannose were found as the commonly utilized substrates.

Brassica rapa subsp. pekinensis (배추) 근권에 서식하는 종속영양세균의 군집 밀도 및 군집수준 생리활성을 2곳의 지역에서 각각 측정하였다. 근권의 종속영양세균군집 평균 밀도는 수원이 $2.65\times10^6CFU\;g^{-1}$ soil, 예산이 $3.75\times10^6CFU\;g^{-1}$ soil로 나타났고, 토양은 수원이 $2.45\times10^6CFU\;g^{-1}$ soil, 예산이 $2.97\times10^6CFU\;g^{-1}$ soil로 각각 나타났다. 평균 기능풍부도(functional richness)는 수원이 90.8, 예산이 154.1로 각각 나타났다. 군집 밀도와 기능 풍부도 사이에는 밀접한 상관관계가 보였다. 양쪽 근권에서 가장 활발하게 분해된 기질은 adonitol, L-asparagine, D-gluconic acid, L-glutamic acid와 D-galacturonic acid 등이었으나, 수원과 예산 근권에서의 기질 분해 양상은 뚜렷한 차이를 보였다. 한편 두 곳의 토양 역시 어느 정도 차이를 보였으나, D-raffinose 및 D-mannose는 공통적으로 잘 분해되는 기질로 나타났다.

Keywords

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