• Title/Summary/Keyword: L-ME

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Effect of Methyl Jasmonate on Ethylene Production in Mungbean Hypocotyls and Leaf Segments (녹두 하배축과 잎에서의 에틸렌 생성에 대한 Methyl Jasmonate의 효과)

  • 이규승
    • Journal of Plant Biology
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    • v.37 no.4
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    • pp.445-452
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    • 1994
  • Effects of methyl jasmonate (MeJA) on ethylene production in mungbean (Phaseolus radiatus L.) hypocotyl and leaf segments were studied. Ethylene production in mungbean hypocotyl segments was decreased in proportion to MeJA concentrations and $450\;\mu\textrm{M}$ of MeJA showed 50% inhibitory effect. This inhibitory effect appeared after 3 h of incubation period and continued for 24 h. Inhibition of ethylene production by MeJA was due to the decrease in 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase activity. However, MeJA treatment had no effect on ACC content and ACC synthase activity. MeJA also inhibited auxin-induced ethylene production in hypocotyls. To investigate the mechanisms of the inhibitory effect of MeJA on the auxin-induced ethylene production, ACC synthase and ACC oxidase activity were examined after MeJA treatment. MeJA decreased the ACC content and ACC synthase activity as weD as ACC oxidase activity in the auxin-treated tissue. These results suggest that the inhibition of MeJA on auxin-induced ethylene production is not due to the direct inhibitory effect of MeJA on the ACC synthase, but to the inhibition of the ability of IAA to promote the synthesis of ACC synthase. In contrast, ethylene production from the detached mungbean leaves was stimulated by MeJA. The rate of ethylene production increased approximately 65% over the control after 12 h of incubation period by $4.5\;\mu\textrm{M}$ MeJA. When MeJA was applied to detached leaves along with IAA, the effect of MeJA appeared to be additive. In an effort to elucidate mechanisms of MeJA action on auxin-induced ethylene production in the leaf tissue, enzyme activities of ACC synthase and ACC oxidase were examined. MeJA stimulated ACC oxidase activity but did not affect ACC synthase activity in leaf tissue. Together, these results suggest that MeJA plays different roles in the ethylene production in the different mungbean tissues.issues.

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A Study on the Forms of the Baroque Costume -Under the rein of Louis XIV- (Baroque 시대의 복장 형태에 관한 연구 -Louis XIV 세 시대를 중심으로-)

  • 김옥진
    • Journal of the Korean Home Economics Association
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    • v.18 no.4
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    • pp.1-11
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    • 1980
  • A la suite de notre essal " Etude sur le Costume Byzantin", o nous avions d cel le probl me du costume au niveau de l'art pur qui r pond un besoin primitif consistant ext rioriser et concr tiser les id es dans l'esprit humain, nous avons essay cette fois de consid rer le m me probl me, c'est- -dire le probl me de composition de formes des costumes, travers ceux de Louis XIV et la classe des nobles, relativement l'arri re-plans de la soci t . Le style de costumes baroques consiste pritncipalement embellir l' tre de roi, pare la vie de palais et composer les costumes de la classe des nobles pour accentuer leurs luxe, majest , l gance, dignit etc., tant bas sur r gime monarchique et mercantilisme,en les faisant avec le brocart, le velours et la soierie de couleurs somptueuses, enjoliv s de broderie, ruban et dentelle.

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Comparison of Parameter Estimation Methods in A Kappa Distribution

  • Jeong, Bo-Yoon;Park, Jeong-Soo
    • 한국데이터정보과학회:학술대회논문집
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    • 2006.04a
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    • pp.163-169
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    • 2006
  • This paper deals with the comparison of parameter estimation methods in a 3-parameter Kappa distribution which is sometimes used in flood frequency analysis. The method of moment estimation(MME), L-moment estimation(L-ME), and maximum likelihood estimation(MLE) are applied to estimate three parameters. The performance of these methods are compared by Monte-carlo simulations. Especially for computing MME and L-ME, ike dimensional nonlinear equations are simplied to one dimensional equation which is calculated by the Newton-Raphson iteration under constraint. Based on the criterion of the mean squared error, the L-ME is recommended to use for small sample size $(n\leq100)$ while MLE is good for large sample size.

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Local Expression of $Mel_{la}$ and Effect of Melatonin on Expression of PLP-A Gene in the Rat Placenta (흰쥐 태반에서의 $Mel_{la}$ 유전자 발현과 멜라토닌이 PLP-A 유전자 발현에 미치는 영향)

  • Shin, Chang-Sook;Lee, Chae-Kwan;Kang, Han-Seung;Kim, Haekwon;Yoon, Yong-Dal;Moon, Deog-Hwan;Kang, Sung-Goo
    • Development and Reproduction
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    • v.5 no.2
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    • pp.181-187
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    • 2001
  • Seasonal changes and circadian rhythm of plasma prolactin(PRL) concentration in mammals are mediated by melatonin. Pinealectomy or denervation of the pineal gland produces an increase in plasma PRL level. In the rat placenta several members of the PRL family gene are expressed during the late pregnancy. However, the full spectrum of their expression mechanisms and regulatory factors are not elucidated yet. Present study aimed to investigate the local expression of the melatonin receptor la(Me $l_{la}$ ) gene and the effect of melatonin on expression of prolactin-like protein A(PLP-A), a member of the PRL-family gene in the rat placenta. According to the RT-PCR, northern blot and in situ hybridization experiments, Me $l_{la}$ gene was locally expressed in the rat placenta, Me $l_{la}$ mRNA was localized mainly in the placental junctional and labyrinth zones. Interestingly, junctional zone of the placenta showed strong expression of Me $l_{la}$ at daytime(16:00) than at nighttime(22:00). Melatonin agonist, chlorornelatonin decreased the PLP-A mRNA levels in the rat placenta. These results suggest that melatonin coupled with Me $l_{la}$ , may act as a regulation factor that mediates the expression of the PLP-A gene in the rat placenta.

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Antiradical Capacities of Perilla, Sesame and Sunflower Oil

  • Hong, Sun-Hee;Kim, Mi-Jin;Oh, Chan-Ho;Yoon, Suk-Hoo;Song, Yeong-Ok
    • Preventive Nutrition and Food Science
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    • v.15 no.1
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    • pp.51-56
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    • 2010
  • The aim of this study is to examine the radical scavenging activity of perilla and sesame oil that Koreans traditionally consume. For DPPH radical scavenging activity, oil and its hexane/70% methanol extracts (ME) are used and for superoxide and hydroxyl radical scavenging activities, ME are used. Unrefined perilla oil, sesame oil, and refined sunflower oil are used. The yields for ME of perilla, sesame and sunflower oil are 0.57, 0.61, and 0.30%, respectively, and the amounts of phenolic compounds in ME of corresponding oil are 18.77, 88.64 and $0.05\;{\mu}g$ tannic acid/mg, respectively. $IC_{50}$ for DPPH scavenging activity of perilla, sesame and sunflower oil are 2.12, 1.91, and 3.35 mg/mL, respectively and those for ME of corresponding oils are 0.42, 0.07, and 43.11 mg/mL, respectively. In DPPH assay, the solvent used for oil sample is iso-octane and that for ME is methanol. Superoxide anion scavenging activity of ME of perilla, sesame and sunflower oil tested at 1 mg/mL concentration are 21.10, 13.25, and 3.14%, respectively. Hydroxyl radical scavenging activities of those samples tested at 1 mg/mL concentration are 86.08, 93.30, and 93.17%, respectively. In summary, the refining process seems to remove the phenolic compound during oil processing. Antiradical substances in perilla and sesame oils responsible for scavenging DPPH radicals are present in the methanol fraction, while the antiradical substances in the sunflower oil are in the lipid fraction. DPPH scavenging activity of ME of sesame oil is significantly higher than that of perilla oil (p<0.05). However, superoxide anion scavenging capacity of ME of perilla oils was found to be greater than that of both sesame and sunflower oils (p<0.05).

Effect of Explant and Cultivars on the Adventitious Shoot Differentiation by Invitro Culture of Narcissus (배양재료와 품종이 수선의 기내배양시 부정아 형성에 미치는 영향)

  • 정향영;한봉희
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.2
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    • pp.103-106
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    • 1997
  • In order to establish a micropropagation system of Naricissus, the ability of bulblet regeneration among propagation materials was compared, and the adequate growth regulators and concentrations for each cultivar were investigated. The inorganic components were also assayed in the parts of propagation materials. In propagation materials, scape with based plates showed hightest rate of bulblet formation and rapid growth of formed bulblets in vitro, comparing to other parts of it. In comparing of varieties, 'Dutch Master' and 'Golden Harvest' showed a high ability for bulblet regeneration. The ability of bulblet regeneration was most favorable in the medium, supplemented with 5.0 mg/L BA and 2.5 mg/L NAA in 'Dutch Master', and 5.0 mg/L BA and 1.0mg/L NAA in 'Golden Harvest', respectively. In inorganic component analysis of propagation materials, the White part of scape contained 1.18 mg/L$P_2O_5$, 2.57 me Ca, 0.94 me Mg and 3.20 mg/L total N. It showed higher levels in concentration of inorganic components as compared to those of the other part of scape. In addition, leaves and yellow part of scape contained significantly high levels of Ca and Mg while scales bulb showed considerably low levels in all inorganic compounds.

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Effect of multi-enzymes supplementation on growth performance, meat quality, ileal digestibility, digestive enzyme activity and caecal microbiota in broilers fed low-metabolizable energy diet

  • Yaqoob, Muhammad Umar;Yousaf, Muhammad;Iftikhar, Mubashir;Hassan, Safdar;Wang, Geng;Imran, Safdar;Zahid, Muhammad Umer;Iqbal, Waqar;Wang, Minqi
    • Animal Bioscience
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    • v.35 no.7
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    • pp.1059-1068
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    • 2022
  • Objective: This study was conducted to evaluate the effect of using low energy diet with multi-enzymes supplementation on different biological parameters in broilers. Methods: Three hundred Arbor Acres broiler chicks were randomly divided into three groups (Cont, standard metabolizable energy(ME); L-ME, ME reduced by 50 kcal/kg without enzyme; and L-ME-MES, L-ME diet was supplemented with multi-enzymes) with five replicates per group (20 chicks per replicate) at the start of second week. Grower and finisher diets were formulated according to breed specific guide and offered with free access in respective phase (two weeks for grower [8 to 21 d]; two weeks for finisher [22 to 35 d]). External marker method was used to measure the nutrient digestibility. After feeding trial, fifteen birds (one bird per replicate) were selected randomly and slaughtered for samples collection. Results: The results exhibited no effect (p>0.05) of dietary treatments on all parameters of growth performance, carcass traits, relative weight of internal organs except bursa and overall parameters of thigh meat quality. Relative weight of bursa was significantly (p<0.05) higher in L-ME than control. Multi-enzymes supplementation in low-ME diet significantly (p<0.05) improved the breast meat pH 24 h, digestibility of crude protein, duodenum weight and length, jejunal morphology, counts of Lactobacillus spp. and Bifidobacterium spp., lipase and protease activities than control. Jejunum length was increased in both L-ME and L-ME-MES treatments than that of the control (p<0.05). Breast meat cooking loss and color lightness was lower in L-ME (p<0.05) than control. Conclusion: It can therefore be concluded that broilers could be reared on low energy diet with supplementation of multi-enzymes without compromising the growth performance. In addition, it is beneficial for other biological parameters of broilers.

Effects of Meretrix Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity

  • Leem, Kang-Hyun
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.3
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    • pp.528-532
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    • 2011
  • This study was designed to investigate the collagen metabolism and tyrosinase activity of Meretrix extracts (ME). The effect of ME on type I procollagen production and collagenase activity in human normal fibroblasts HS68 after UVB (312 nm) irradiation was measured by ELISA method. The tyrosinase activity after treatment of ME was measured as well. Type I procollagen production was recovered by ME in UVB damaged HS68 cells. The increased collagenase activity after UVB damage was significantly recovered by ME. The tyrosinase activity and L-DOPA oxidation were significantly reduced as well. However, the effects on tyrosinase activity and L-DOPA oxidation were not powerful enough to be used as whitening agents. ME showed the anti-wrinkle effects and some whitening effects in vitro. These results suggest that ME may be a useful drug as an anti-wrinkle treatments.

Comparisons of Collection 5 and 6 Aqua MODIS07_L2 air and Dew Temperature Products with Ground-Based Observation Dataset (Collection 5와 Collection 6 Aqua MODIS07_L2 기온과 이슬점온도 산출물간의 비교 및 지상 관측 자료와의 비교)

  • Jang, Keunchang;Kang, Sinkyu;Hong, Suk Young
    • Korean Journal of Remote Sensing
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    • v.30 no.5
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    • pp.571-586
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    • 2014
  • Moderate Resolution Imaging Spectroradiometer (MODIS) provides air temperature (Tair) and dew point temperature (Tdew) profiles at a spatial resolution of 5 km. New Collection 6 (C006) MODIS07_L2 atmospheric profile product has been produced since 2012. The Collection 6 algorithm has several modifications from the previous Collection 5 (C005) algorithm. This study evaluated reliabilities of two alternative datasets of surface-level Tair and Tdew derived from C005 and C006 Aqua MODIS07_L2 (MYD07_L2) products using ground measured temperatures from 77 National Weather Stations (NWS). Saturated and actual vapor pressures were calculated using MYD07_L2 Tair and Tdew. The C006 Tair showed lower mean error (ME, -0.76 K) and root mean square error (RMSE, 3.34 K) than the C005 Tair (ME = -1.89 K, RMSE = 4.06 K). In contrasts, ME and RMSE of C006 Tdew were higher than those (ME = -0.39 K, RMSE = 5.65 K) of C005 product. Application of ambient lapse rate for Tair showed appreciable improvements of estimation accuracy for both of C005 and C006, though this modification slightly increased errors in C006 Tdew. The C006 products provided better estimation of vapor pressure datasets than the C005-derived vapor pressure. Our results indicate that, except for Tdew, C006 MYD07_L2 product showed better reliability for the region of South Korea than the C005 products.

Effects of added chlorides and their concentrations on growth and nitrate content in leaf lettuce(Lactuca sativa L.) in hydroponics.

  • Lee, Guang-Jae;Shin, Hyun-Man;Kim, Ki-Sik;Lee, Cheol-Hee;Kim, Jin-Han
    • Plant Resources
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    • v.2 no.1
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    • pp.26-30
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    • 1999
  • Nitrate in edible vegetables is converted to nitrite by nitrate reductase(NR) and/or bacteria in intestines. Nitrite and amino, in the intestine of some animals and human, bind to form nitrosamine, which is toxic and known as carcinogen. This study was carried out to examine the effect of added chlorides and their concentrations on growth, yield and nitrate content in leaf lettuce plants in hydroponics. Seeds of lettuce cv, "Samsunjokchukmyon" were planted on April 29, and seedlings were planted on June 2, and were cultured until July 5 in 1998. KCI and CaCl₂ were used as chloride source and their concentrations were 1, 2 and 4 me/L, respectively, in the lettuce standard nutrient solution for National Horticultural Research Institute(NHRI). Completely randomized design with 3 replications was used. Nitrate content and NR activity were measured 2 and 5 weeks after planting(WAP). The obtained results were summarized as the follows : Leaf weight per plant was difference from harvest dates and treatments, but total leaf weight was not significantly different among treatments. Number of leaves was higher in KCI 2 me/L, CaCl₂1 me/L and control at 2 WAP than the others, and was higher in KCI 1 me/L, and control at 3 WAP than others, and was higher in control at 5 WAP. Total number of harvested leaves was the highest in control with 14, which followed by KCI 2 me/L and CaCl₂1 me/L. Nitrate content was decreased by addition of chloride in nutrient solution. Nitrate content in the 3rd and 9th leaves was significantly decreased. NR activity was higher in control and CaCl₂ addition treatments, while KCI addition treatments reduced NR activity. However, no direct relationship with nitrate was observed. Growth characteristics such leaf length and leaf width were not significantly influenced by chloride addition.

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