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유도 당기기.후리기 훈련 및 측정 장비 개발 (The Instrumental Development for Pulling.Reaping Training & Measuring in Judo)

  • 김의환;최은수;남덕현;김정섭;정재욱;김태완
    • 한국운동역학회지
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    • 제18권1호
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    • pp.213-226
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    • 2008
  • 본 연구의 목적은 유도의 기본 기술인 당기기, 후리기 훈련 및 측정이 가능한 사람-받기(파트너) 역할을 대신할 수 있는 장비인 유도 인형(doll-uke, 장비-받기)을 개발하는데 있다. 기본 방향은 사람-받기와 최대한 유사한 구조와 기능을 갖도록 하고, 절차는 [기본 모형 설계-제작-평가(1)-모형(2) 평가(2)-1차 제작-보완-2차 제작-보완-평가(3)-완성(최종 제작)- 데이터 취득 장치 연결-완성]의 단계를 거쳤다. 평가는 사람-받기와 장비-받기를 상대로 하는 경우, 당기기 밧다리후리기 동작에 대한 역학적 유사성을 위해 각 변인의 상대수준차를 계산하였으며, 선수들과 지도자의 설문 평가 단계를 거쳤다. 개발된 장비-받기의 구조는 크게 유도인형(doll-uke)과 힘 측정시스템(doll-uke fore system)이다. 밧다리후리기 발휘, 시 사람을 대신한 장비-받기의 역학적 변인들에서 재현율이 관찰되었으며, 동작 패턴이 사람-받기의 유사함과 설문 평가에서도 유용성이 인정되었다. 사람-받기를 대신할 훈련 및 측정 장비인 유도 인형(doll-uke)을 이용함으로서 당기기 밀기 후리기 등의 기술 강화는 물론, 동작 시 발휘되는 힘의 정량적 데이터를 용이하게 얻어 선수들에게 피드백을 제공해줌으로써, 정성적 정량적 분석을 통한 훈련 현장의 과학화, 경기력 향상을 기대할 수 있겠다.

반추동물 신증의 조기진단을 위한 뇨효소 측정법 1. 효소활성도 측정을 위한 적합한 조건과 정상범위에 관하여 (Measurement of Urine Enzymes for the Early Diagnosis of Nephrosis in Ruminants 1. Optimal Conditions for Measurement of Enzyme Activities and Normal Ranges)

  • 이창우;이경갑
    • 한국임상수의학회지
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    • 제6권2호
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    • pp.291-305
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    • 1989
  • Present experiment was performed to establish the optimal reaction conditions for measurement of urinary gamma-glutamyltranspeptidase(${\gamma}$-GTP), N-acetyl-${\beta}$-D-glucosaminidase (AGS) and alanine aminopeptidase(AAP) activities in bovine and to investigate in vitro stability of the enzymes, within-run imprecision of the methods, and normal ranges. 1. The optimal wavelength for measurement of ${\gamma}$-GTP activity was 545nm. 2. The optimal pH of Tris-HCI buffer containing glycylglycine for measurement of urinary ${\gamma}$-GTP activity was 7.6~7.8(37$^{\circ}C$). 3. Coefficient of variance for within-run imprecision of urinary ${\gamma}$-GTP activity ranged from 4.8 to 7.2% and there was no significant difference among replications, 4. The optimal wavelength for measurement of urinary AGS activity was 405nm. 5. The optimal pH of citrate buffer for measurement urinary of AGS activity was 4.0(37$^{\circ}C$). 6. Coefficient of variance for within-run imprecision of urinary AGS activity ranged from 3.9 to 6.1% and there was no significant difference among replications. 7. The optimal wavelength for measurement of urinary AAP activity was 400nm. 8. The optimal pH of phosphate buffer for measurement of urinary AAP was 7.8. 9. Coefficient of variance for within-run imprecision of urinary AAP activity ranged from 2.5 to 4.8% and there was no significant difference among replications. 10. ${\gamma}$-GTP and AGS activities were increased significantly by gel-filtration. 11. Turbidity interfered with measurement of urinary AAP activity in bovine unless the specimen was gel-filterated. 12. Preservation of the specimen at 5$^{\circ}C$ or -20$^{\circ}C$ did not affect the AGS activity at least for 7 days after collection. 13. Preservation of the specimen at 5$^{\circ}C$ or 20$^{\circ}C$ did not affect the ${\gamma}$-GTP and AAP activities statistically, but some individual specimens revealed fluctuation during preservation. 14. ${\gamma}$-GTP, AGS and AAP activities revealed fluctuation by the tine of the day when the specimen was collected. 15. The normal ranges of urinary ${\gamma}$ -GTP, AGS and AAP activities were 6.60${\pm}$3.26(2.36-14.50), 1.31 ${\pm}$ 0.81(0.33-3.78), and 1.73 ${\pm}$ 0.55(0.77-3.03)U/l. respectively.

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아메리카잎굴파리 (Liriomyza trifolii)의 외부기생봉 굴파리좀벌 (Diglyphus isaea)의 생태적 특성 및 저장 조건 구명 (Ecological Characteristics and Storage Condition of Diglyphus isaea(Walker) (Hymenoptera: Eulophidae), an Ectoparasite of Liriomyza trifolii(Burgess) (Diptera: Agromyzidae))

  • 김정환;변영웅;김용헌;김황용
    • 한국응용곤충학회지
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    • 제46권1호
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    • pp.43-49
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    • 2007
  • 아메리카잎굴파리 외부기생봉인 굴파리좀벌의 온도별 알, 유충, 번데기 발육기간과 성충의 산란수, 기주체액 흡즙수, 성충수명, 저온 저장력 등을 조사하였다. 굴파리좀벌의 알기간, 유충기간, 번데기 기간은 $25^{\circ}C$에서 각각 1.3일, 4.5일, 6.3일이었다. 성충의 산란수, 기주체액 흡즙수, 성충수명은 $25^{\circ}C$에서 각각 305.3마리, 657.0마리, 25.1일이었다. 아메리카잎굴파리 유충 영기별 굴파리좀벌의 산란선호성과 흡즙선호성을 조사한 결과 각각 90.2%와 82.8%가 3령 유충에 집중되었다. 굴파리좀벌의 저온저장에 따른 50% 생존기간은 꿀물 20%희석 용액을 제공할 경우 $5^{\circ}C,\; 10^{\circ}C,\;15^{\circ}C$에서 각각 180일, 150일, 90일이었다. 굴파리좀벌 저온저장 후 산란수는 저장기간 30일, 70일, 140일에서 각각 190.8개, 104.0개, 89.4개였다.

Dry Matter Digestion Kinetics of Two Varieties of Barley Grain Sown with Different Seeding and Nitrogen Fertilization Rates in Four Different Sites Across Canada

  • Cleary, L.J.;Van Herk, F.;Gibb, D.J.;McAllister, T.A.;Chaves, A.V.
    • Asian-Australasian Journal of Animal Sciences
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    • 제24권7호
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    • pp.965-973
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    • 2011
  • Our objective was to determine the differences in the rate and extent of dry matter digestion between barley subjected to differing agronomic variables. Two malting barley varieties, Copeland and Metcalfe were seeded at rates of 200 and 400 plants/$m^2$. Each of these varieties received nitrogen fertilizer at rates of 0, 30, 60 and 120 kg/ha, resulting in a total of 20 different barley grain samples. Samples were ground through a 6mm screen and approximately 3 g of each weighed into 50 ${\mu}m$ Dacron bags and sealed. The bags were incubated in three ruminally cannulated Holstein cattle for periods of 0, 3, 6 and 24 h. Using the data obtained from these incubations, rates of digestion were able to be predicted. The soluble fraction ranged from 0.229-0.327, the slowly degradable fraction ranged from 0.461-0.656, and the undegradable fraction ranged from 0.038-0.299. The rates of digestion ranged from 0.127-0.165 $h^{-1}$ and the effective degradability ranged from 0.527-0.757. At the Canora location, the Copeland samples which received 120 kg/ha of nitrogen fertilizer had a significantly lower (p = 0.013) soluble fraction than the rest of the samples at that location. A significant interaction (p = 0.009) was seen between the seeding rate and nitrogen fertilizer application with samples from the Canora location, as well as significant differences (p = 0.029) between nitrogen application rates in samples from the Indian head location. The rate of digestion of samples from the Indian head location differed (p = 0.020) between the two seeding rates, with samples seeded at 200 seed/$m^2$ having a slightly higher rate of degradation. Differences in the effective degradability were seen between the different nitrogen application rates with samples from both the Canora and Indian head locations, as well as an (p = 0.004) interaction between the seeding rate and nitrogen fertilizer application rate. Although there was not a clear correlation between the different variables, both nitrogen application and seeding rate did have a significant effect on the rates and extent of digestion across each of the four locations.

광어(Paralichtys olivaceus)의 스쿠치카감염증(感染症) -스쿠치카섬모충(纖毛蟲)의 배양성상(培養性状).약제감수성(藥劑感受性).병원성(病源性)-

  • 길수 수;일향진일;오 명주;생도삼내자;목촌교구;삼 립성;야촌철일;회면량남
    • 한국어병학회지
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    • 제6권2호
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    • pp.205-218
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    • 1993
  • On the development of hirame(Paratichtys olivaceus) culture, outbreak of scuticociliata infection was reported to cause severe damage in Japan. To establish effective measures for isolation and cultivation of this ciliate, we tried to culture this pathogenic ciliate using medium for bacteria and fish cell lines in vitro. Scuticociliata from the brain tissues of infected fish was aseptically inoculated to CHSE-214 cells cultured in MEM-10 without antibiotic. Scuticociliata grew well and the number of ciliate reached $10^6\;cells/ml$ at temperatures of $15^{\circ}C$ to $20^{\circ}C$ for 10d. The number of ciliate cultured in the cell lines is 10 times higher than the numbers cultured in the liquid medium alone. This ciliata could be cloned by dilution method. Scuticociliata isolated could grow well on 42 different cell lines that were established from marine fish, warm freshwater fish, and salmonids. This ciliate could be preserved in liquid nitrogen for more than 6 months. Subsequently, we observed the optimal temperature and salinity for growth, and tested the sensitivities of this organism to formaldehyde, flagyl(Metronidazole), Ekuteshin(Combination compound of sulfamonometoxin and ormethoprim), and ozonixation. Optimal temperature for growth was $25^{\circ}C$ and salinity was 1.0 to 1.5%. Washed scuticociliata was killed by formaldehyde at the concentration of 50ppm for 10min, but was not completely killed even at a high concentration of 400ppm for 20min in MEM-5. Flagyl and Ekuteshin can inhibit the growth of scuticociliata at the concentration of 1,000 and $100{\mu}g/ml$ in MEM-10, respectively. More than 99% of this scuticociliata could be killed by ozonization at a dose equivalent to $1.0mg/\ell$ oxidant for 30sec in sea water. Isolated scuticociliata showed the pathogenicity to the cultured hirame by artificial infection(I. P. injection, $10^5\;cells$/fish). The number of scuticociliata in the water could be counted by most probable number(MPN) method using tissue culture, and the minimum detectable number was $1.8\;cells/\ell$. The number in the reservoir tank for water supply to the culture tank was 110 cells/l. After cleaning by elimination of the sediments from of the reservoir tank and disinfected with formaldehyde, number of scuticociliata decreased and was counted less than $1.8\;cells/\ell$ and infection rate of cultured hirame was decreased.

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주요(主要) 밭잡초(雜草) 종자(種子)의 발아(發芽) 및 출아(出芽)에 관(關)한 연구(硏究) II. 복토(覆土) 심도(深度), 산도(酸度), 시비(施肥)가 잡초종자(雜草種子)의 출아(出芽)에 미치는 영향(影響) (Germination and Emergence of Major Upland Weeds II. Effects of Soil Depth, pH and Fertilization on Emergence of Weeds)

  • 우인식
    • 한국잡초학회지
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    • 제11권3호
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    • pp.224-228
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    • 1991
  • 주요 밭잡초(雜草)의 복토심도(覆土深度), 산도(酸度), 시비(施肥)가 출아(出芽)에 미치는 영향(影響)을 알기위해 시험(試驗)을 수행(遂行)한 결과(結果)는 l. 밭토양(土壤)의 토심별(土心別) 잡초종자(雜草種子)의 분포(分布)는 10cm까지 58%가 존재(存在)하였고 40-45cm층(層)에도 2%가 분포(分布)하였으며 2. 복토(覆土)깊이에 따른 출아(出芽)에서 냉이는 표면(表面) 파종(播種)에서, 털비름, 개비름, 쇠비름, 명아주, 까마중은 3cm까지, 왕바랭이, 피는 7cm까지 출아(出芽)하였으며 강아지풀과 피는 7cm깊이에서도 각각(各各) 26, 40%의 높은 출아율(出芽率)을 보였다. 3. 공시(供試)된 잡초(雜草) 종자(種子)의 출아(出芽)는 pH 2.0-3.0의 탄산성(彈酸性)에서는 출아(出芽)가 저조했으나 피, 강아지풀, 명아주는 출아(出芽)했으며 pH 3.5-4.0 이상(以上)에서는 출아(出芽)에 큰 영향(影響)이 없었던 것으로 사료(思料)된다. 4. 토성간(土性間) 출아(出芽)는 토양(土壤)에서 왕바랭이, 냉이, 털비름, 개비름, 명아주, 피, 까아중, 강아지풀이 좋았으며 사양토(砂壤土)에서는 쇠비름, 바랭이가 좋았으나 시비(施肥) 유무간(有無間)에는 출아(出芽)에 영향(影響)을 주지 않았다.

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Changes in Phytoavailability of Cadmium, Copper, Lead, and Zinc after Application with Eggshell in Contaminated Agricultural Soil

  • Kim, Rog-Young;Yang, Jae E.
    • 한국토양비료학회지
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    • 제47권1호
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    • pp.41-47
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    • 2014
  • Agricultural soils surrounding mine areas in South Korea are often contaminated with multiple metals such as Cd, Pb and Zn. It poses potential risks to plants, soil organisms, groundwater, and eventually human health. The aim of this study was to examine the changes in phytoavailability of Cd, Cu, Pb and Zn after application with calcined eggshell (CES; 0, 1, 3, and 5% W/W) in an agricultural soil contaminated by mine tailings. The contents of Cd, Cu, Pb and Zn in soils were 8.79, 65.4, 1602, and $692mgkg^{-1}$ (aqua regia dissolution), respectively. The experiments were conducted with lettuce (Lactuca sativa L. var. longifolia) grown under greenhouse conditions during a 30-d period. $NH_4NO_3$ solution was used to examine the mobile fraction of these metals in soil. The application of CES dramatically increased soil pH and inorganic carbon content in soil due to CaO and $CaCO_3$ of CES. The increased soil pH decreased the mobile fraction of Cd, Pb, Zn: from 3.49 to < $0.01mgkg^{-1}$ for Cd, from 79.4 to $1.75mgkg^{-1}$ for Pb, and from 29.6 to $1.13mgkg^{-1}$ for Zn with increasing treatment of CES from 0 to 5%. In contrast, the mobile fraction of Cu was increased from 0.05 to $3.08mgkg^{-1}$, probably due to the formation of soluble $CuCO_3{^0}$ and Cu-organic complex. This changes in the mobile fraction resulted in a diminished uptake of Cd, Pb and Zn by lettuce and an increased uptake of Cu: from 4.19 to < $0.001mgkg^{-1}$ dry weight (DW) for Cd, from 0.78 to < $0.001mgkg^{-1}$ DW for Pb, and from 133 to $50.0mgkg^{-1}$ DW for Zn and conversely, from 3.79 up to $8.21kg^{-1}$ DW for Cu. The increased contents of Cu in lettuce shoots did not exceed the toxic level of $>25mgkg^{-1}$ DW. The mobile contents of these metals in soils showed a strong relationship with their contents in plant roots and shoots. These results showed that CES effectively reduced the phytoavailability of Cd, Pb, and Zn to lettuce but elevated that of Cu in consequence of the changed binding forms of Cd, Cu, Pb, and Zn in soils. Based on these conclusions, CES can be used as an effective immobilization agent for Cd, Pb and Zn in contaminated soils. However, the CES should be applied in restricted doses due to too high increased pH in soils.

Preparation and Evaluation of a 4-Branched Polyethylene Glycol Derivative Modified with Exendin-4 and Stearylamine for Extended Hypoglycemic Action

  • Kim, In-Soo;Ma, Kyung-Wan;Bae, Sung-Ho;Yoon, Jeong-Hyun;Oh, Kyung-Taek;Lee, Eun-Seong;Lee, Don-Haeng;Lee, Kang-Choon;Youn, Yu-Seok
    • Journal of Pharmaceutical Investigation
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    • 제40권3호
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    • pp.175-180
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    • 2010
  • Albumin-modification has been viewed as one of the most effective ways of extending the short in vivo lifetimes of peptide drugs by delaying glomerular filtration. In this study, we describe a new type 2 anti-diabetic exendin-4 (Ex4) peptide derivative with significant binding ability to human serum albumin (HSA). This exendin-4 derivative consists of a 4-branched polyethylene glycol $(PEG)_{5k}$ (Mw: 20 kDa) modified with three stearylamines ($C_{18}-NH_2$) and one exendin-4 on its branches. PEG and stearylamine were selected to provide functionality to increase molecular size and bind to albumin, respectively. This derivative ($3C_{18}-4PEG_{5k}$-Ex4) was shown to have larger molecular size (Ca. 152 kDa) than actual (25.0 kDa) when subjected to size-exclusion chromatography, and the fluorescein-tagged $3C_{18}-4PEG_{5k}$-Ex4 displayed significant binding to the HSA-immobilized Sepharose CL-4B resin using confocal laser scanning microscopy. Furthermore, $3C_{18}-4PEG_{5k}$-Ex4 was found to have acceptable anti-hyperglycemic efficacy via three consecutive oral glucose tolerance testings (OGTT) in fasted type 2 diabetic db/db mice. The $HD_{total}$ value ($57.6{\pm}12.3%$) of $3C_{18}-4PEG_{5k}$-Ex4 at a 50 nmol/kg dose was 2-fold greater than that ($31.0{\pm}8.7%$) of native exendin-4 in non-fasted db/db mice. Especially, the blood glucose levels in the mice group treated with $3C_{18}-4PEG_{5k}$-Ex4 did not rebound to ~150 mg/dL until 24 h after the injection, which obviously shows the extended hypoglycemia. We believe that this derivative has great pharmaceutical potential as a novel long-acting type 2 anti-diabetic injection treatment.

Effect of Dietary Lysine Restriction and Energy Density on Performance, Nutrient Digestibility and Meat Quality in Finishing Pigs

  • Jin, Y.H.;Oh, H.K.;Piao, L.G.;Jang, S.K.;Choi, Y.H.;Heo, P.S.;Jang, Y.D.;Kim, Y.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권9호
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    • pp.1213-1220
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    • 2010
  • This experiment evaluated the effects of dietary lysine restriction and energy density on growth performance, nutrient digestibility and meat quality of finishing pigs. A $2{\times}2$ factorial arrangement of treatments was utilized in a randomized complete block (RCB) design, and factor 1 was lysine restriction and factor 2 was energy density. The control diet was formulated to contain 3.265 Mcal of ME/kg, 0.75% lysine in the early-finishing phase and 3.265 Mcal of ME/kg, 0.60% lysine in the late-finishing phase and other nutrients met or exceeded NRC (1998) standards. Compared to the control diet (CON), lysine levels of experimental diets were restricted to 15% (treatment EL, EEL) or 30% (treatment ELL, EELL), whereas energy level of experimental diets was increased by 0.100 or 0.200 Mcal of ME/kg. A total of 100 crossbred pigs ([Yorkshire${\times}$Landrace]${\times}$Duroc), with average initial body weight of $58.47{\pm}1.42\;kg$, were allotted to 5 dietary treatments based on sex and body weight. Each treatment had 5 replicates with 4 pigs (two barrows and two gilts) per pen. ADG, ADFI and feed efficiency were calculated in an 8-week growth trial. In the late finishing period (5-8 weeks), pigs fed ELL or EELL diets had decreased ADG and feed efficiency (p<0.01), however, when the EEL diet was provided, a similar growth performance was observed compared to those fed the CON diet during the whole experimental period (p>0.05). In a metabolic trial, 15 pigs were used to evaluate the effect of dietary lysine restriction and energy density on nutrient digestibility. The digestibility of dry matter, crude fat and crude ash was not improved by restricting dietary lysine or energy density. However, crude protein digestibility was decreased (p<0.05) as dietary lysine was restricted. When dietary lysine was restricted, fecal nitrogen was increased whereas nitrogen retention was decreased. BUN concentration was affected by dietary lysine restriction; treatments ELL and EELL had higher BUN values than other treatments (p<0.01). Carcass characteristics and meat quality were measured when average body weight of pigs reached $107.83{\pm}1.50\;kg$. Treatment ELL had higher last rib backfat depth (p<0.05) than treatment CON, but ELL and EEL did not differ significantly. The ELL and EEL treatments had higher (p<0.05) subjective marbling score than treatment CON. Treatment EEL showed higher longissimus fat content than treatment EL and CON (p<0.01). The results indicated that finishing pigs fed a diet with 15% lysine restriction and 3.465 Mcal of ME/kg energy density had no detrimental effects on growth performance and N utilization, and could achieve substantial increases in marbling and longissimus fat content of pork.

노인 당뇨환자에서 당뇨 자가관리 지식, 자신감, 행위 및 삶의 질과 당화혈색소 조절의 관련성 (The Relationship between HbA1c Control and Diabetes Self-care Knowledge, Competence, Behavior and Quality of Life on Diabetes elderly)

  • 이송흔
    • 한국산학기술학회논문지
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    • 제18권11호
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    • pp.357-366
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    • 2017
  • 본 연구는 제2형 당뇨노인을 대상으로 당화혈색소 조절군과 비조절군의 당뇨 자가관리 지식, 자가관리 효능감, 자가 관리 행위와 삶의 질을 비교분석하기 위한 연구이다. 자료수집기간은 2015년 4월 20일부터 8월 31일까지로 D시에 소재한 시민건강증진실을 주기적으로 방문하는 60세 이상의 당뇨노인 205명을 대상으로 하였다. 수집된 자료는 SPSS Statistics 23.0의 기술통계, t-test, chi-square 및 ANCOVA를 이용하여 분석하였다. 연구결과, 연구 대상자의 자가관리 지식은 $6.99{\pm}2.17$점, 당뇨 자가관리 자신감은 $71.27{\pm}10.21$점, 당뇨 자가관리 행위는 $62.78{\pm}1.29$점, 삶의 질은 $0.86{\pm}0.11$점으로 조사되었다. 당화혈색소 조절군과 비조절군간에 당뇨 자가관리 지식, 자신감, 삶의 질에는 차이가 없었으나 당뇨 자가관리 행위는 두 군간 유의한 차이가 있었고(t=2.17, p=.031), 자가관리 행위는 교육수준(t=2.17, p=.031), 시민건강증진실 방문횟수(t=16.497, p=.001) 및 BMI(t=.01, p=.012)에서 유의한 차이가 있었다. 그러나, 앞 선 세 변수를 공변량 처리하여 분석을 시행한 결과, 당화혈색소 수치와 당뇨자가간호 행위와의 관계는 유의하지 않았다. 본 연구의결과를 근거로, 당뇨 노인의 지식수준과 노인의 특성을 반영한 자가관리 교육프로그램이 개발되어 적용될 것을 제언한다. 아울러, 지역사회 당뇨 환자들이 바람직한 당뇨 자가관리를 꾸준히 시행할 수 있도록 지도하고 관리할 수 있는 접근성이 높은 건강관리 기관이 도처에 마련될 필요가 있다.