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Effect of Solid Matrix Priming on Peppers(Capsicum annuum L.) Germinability III. Germination Temperature and Duration of Dehydration (Solid Matrix Priming 처리가 고추종자의 발아에 미치는 영향 III. 발아온도와 건조기간)

  • Kim, Do-Hahn
    • Journal of agriculture & life science
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    • v.43 no.5
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    • pp.17-25
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    • 2009
  • The purpose of this study was to find out the optimum germination temperature and dehydration period at SMP processing, and applies 3 kind of seed treatment and compares yield with stand establishment of seeds of 12 pepper cultivars. It was showed that all 12 cultivars of nontreated seed does not entirely germinated in $15^{\circ}C$. It was that SMP seed germination rate 77(Yeomyeong)~100% (Deawang) high, and seeds 12 cultivars was tendency high by more than 93% at all temperatures excepting Bugang($35^{\circ}C$, 85%) and Yeomyeong($15^{\circ}C$, 77%). Dehydration condition of result were Gumsure, Dongbang Jonggagip, Hyangchon, Yeomyeong, Kwangbok, Wang by which 3 hours in $45^{\circ}C$ were proper, which 6 hours in $35^{\circ}C$ were proper Manitta, Joyang, and judged that 12 hours in $25^{\circ}C$ were proper in Bugang, Dabotap, Deawang. It was reported that water percentage of moisture content change of pepper seed by dehydration was difference a little in species, but was judged by 3-6 hours in $45^{\circ}C$. There was compared to SMP, Osmopriming, pH control by stand establishment were shown pH control of Hyangchon, Dabotap species was nontreated, osmopriming treatment. Also, Kwangbok species was tendency that stand establishment was low, and Deawang was good than other species. Yield of nontreated of yield day was tendency, and miss planted rate also appeared high for 90 day. There was difference in species, and processing of pH control and osmopriming was not shown stand establishment that is stabilized in all species. SMP processing condition could bring security of stable stand establishment in all species, and was considered that research that was under various environment condition should be proceeded conclusively.

Studies on the Evaluation of Kenaf as a Bulking Agent in Livestock Composting (가축 분뇨의 퇴비화 수분 조절제로서 Kenaf의 유용성 평가)

  • Lim, Jeong-Ju;Kim, Dong-Hyeok;Lee, Jin-Ju;Kim, Dae-Geun;Lee, Hu-Jang;Min, Wongi;Park, Dongjin;Huh, Moo-Ryong;Chang, Hong-Hee;Kim, Pil-Joo;Kim, Suk
    • Journal of agriculture & life science
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    • v.45 no.2
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    • pp.21-28
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    • 2011
  • Hibiscus cannabinus L is a plant in the Malvaceae family. Kenaf was seeded at June 1st in 2010 and harvested at November 18th and dried and evaluated worth as a bulking agent for livestock composting. Harvested and dried Kenaf was divided into the bast, core and leaf. All materials were grinded by hammer mill and the moisture absorption, moisture evaporation, pH, volume weight and C/N ratio were measured. Kenaf was higher water absorption and evaporation ability than those of sawdust and chaff. The pH values of Kenaf were pH $2.8{\pm}0.01$ - $4.34{\pm}0.02$, which is lower pH value than those of sawdust (pH $5.28{\pm}0.01$) and chaff (pH $6.3{\pm}0.02$). The C/N ratio of Kenaf showed 649 of core, 204 of bast and 70 of leaf, which were lower than sawdust (789.1) but higher than chaff (132). In volume weight test, the materials were divided by particle size of Kenaf, named as group A(${\geq}4cm$), B(${\leq}4cm$, ${\geq}0.25cm$) and C(${\leq}0.25cm$). The volume of weight of group A and B for core, bast and leaf showed similar, but group C showed higher than those of sawdust and chaff. Especially, the volume weight of group C for leaf was 5 times higher than those of sawdust and chaff. This study suggested the possibility of using Kenaf as a bulk agent for composting of livestock manure. This is considered that strengthen the competitiveness of farmers through reducing the cost, prevention of environmental pollution caused by livestock manure and environmentally friendly processing of livestock manure.

Chemical Components and Antimicrobial Activity of Garlics from Different Cultivated Area (산지별 마늘의 화학성분 및 항균활성)

  • Jeong, Chang-Ho;Bae, Young-Il;Lee, Jin-Hwa;Roh, Jeang-Gwan;Shin, Chang-Sik;Choi, Jine-Shang;Shim, Ki-Hwan
    • Journal of agriculture & life science
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    • v.43 no.1
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    • pp.51-59
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    • 2009
  • The chemical components and antimicrobial activities of garlic from different area were investigated and analyzed to provide basic data for functional food materialization and processing. Hunter's values of garlic from different area were L 53.41~57.15, a -3.49~-4.38 and b 11.47~17.55. The moisture, crude protein, crude fat, nitrogen free extract, crude fiber and ash were 65.24~71.96, 6.24~9.35, 0.21~0.49, 19.01~22.72, 0.58~0.95 and 1.01~2.01%, respectively. The major minerals of garlic from different area were Na(27.22~112.03), Mg(18.17~32.56), K(242.16~569.28), Ca(28.60~63.93), P(117.72~265.21 mg%) and major free sugars were sucrose, glucose and fructose. The major amino acids of garlic from different area were proline, arglmne, glutamic acid and aspartic acid and content of total amino acid was 2,709.33~4,561.04 mg%. The ascorbic acid content of garlic from different area was 2.966~8.673 mg%. Composition of fatty acids of garlic from different area were linoleic acid, oleic acid and palmitic acid, unsaturated fatty acid and saturated fatty acid contents were 72.18~74.35 and 25.65~27.82%, respectively. Antimicrobial activities of garlic extracts as different area increased depends on concentration and showed the high antimicrobial activities against Gram(+) and Gram(-).

A New High Qualilty Rice Variety with Lodging Resistance and Multiple Resistance to Diseases, "Donghaejinmi" (중만생 고품질 내도복 복합내병성 벼 신품종 "동해진미(東海珍味)")

  • Yeo, Un-Sang;Kim, Jeong-Il;Lee, Jeom-Sig;Park, No-Bong;Chang, Jae-Ki;Oh, Byeong-Geun;Kang, Jung-Hun;Kwak, Do-Yeon;Cho, Jun-Hyun;Lee, Jong-Hee;Kwon, Oh-Deog;Lee, Ji-Yoon;Nam, Min-Hee;Kim, Sang-Yeol;Ku, Yeon-Chung;Kim, Jae-Kyu
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.288-291
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    • 2009
  • A new commercial rice variety "Donghaejinmi" is a japonica rice (Oryza sativa L.) with lodging resistance and high grain quality. It has been developed by the rice breeding team of Yeongdeog Substation, National Institute of Crop Science (NICS), RDA. This variety was derived from a cross between "Milyang 64" as a resistance source of brown planthopper (Bph) and "Milyang 165" as grain quality source. The donor parent, "Milyang64" has been backcrossed three times with recurrent parent, "Milyang165" and selected by the pedigree breeding method. The pedigree of "Donghaejinmi", designated as "Yeongdeog 41" in 2003, was YR21259-B-B-68-1. It has a short culm length with 69 cm and medium-late growth time. This variety is resistant to stripe virus and moderately resistant to leaf blast disease with durable resistance. It also has tolerance to unfavorable environment such as cold, dried wind and storm. Milled rice kernel of "Donghaejinmi" is translucent, clear in chalkness and good at eating quality in panel test. The merit of this variety is high head rice ratio, which is essential element to produce an article of superior quality rice brand. The yield potential of "Donhaejinmi" in milled rice is about 6.05 MT/ha at ordinary fertilizer level of local adaptability test. This cultivar would be adaptable to Yeongnam inland plains and eastern costal area of Yeongnam province.

Estimation of Rice Grain Protein Contents Using Ground Optical Remote Sensors (지상광학센서를 이용한 쌀 단백질함량 예측)

  • Kim, Yi-Hyun;Hong, Suk-Young
    • Korean Journal of Remote Sensing
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    • v.24 no.6
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    • pp.551-558
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    • 2008
  • It is well known that the protein content of rice grain is an indicator of taste of cooked rice in the countries where people as the staple food. Ground-based optical sensing over the crop canopy would provide information not only on the mass of plant body which reflects the light, but also on the crop nitrogen content which is closely related to the greenness of plant leaves. The vegetation index has been related to crop variables such as biomass, leaf nitrogen, plant cover, and chlorophyll in cereals. The objective of this study was to investigate the correlation between GNDVI and NDVI values, and grain protein content at different dates and to estimate the grain protein content using G(NDVI) values. We measured Green normalized difference vegetation index [$GNDVI=({\rho}0.80{\mu}m-{\rho}0.55{\mu}m)/({\rho}0.80{\mu}m+{\rho}0.55{\mu}m)$] and [$GNDVI=({\rho}0.80{\mu}m-{\rho}0.68{\mu}m)/({\rho}0.80{\mu}m+{\rho}0.68{\mu}m)$] by using two different active sensors. The study was conducted during the rice growing season for three years from 2005 through 2007 at the experimental plots of National Institute of Agricultural Science and Technology. The experiments were carried out by randomized complete block design with the application of four levels of nitrogen fertilizers(0, 70, 100, 130kg N/ha) and the same amount of phosphorous and potassium content of the fertilizers. After heading stage, relationships between GNDVI of rice canopy and grain protein content showed the highly positive correlation at different dates for three years. GNDVI values showed higher correlation coefficients than that of NDVI during growing season in 2005-07. The correlation between GNDVI values at different dates and grain protein contents was highly correlated at early July. We attempted to estimate the grain protein content at harvesting stage using GNDVI values from early July for three years. The determination coefficients of the linear model by GNDVI values were 0.9l and the measured and estimated grain protein content at harvesting stage using GNDVI values highly correlated($R^2=0.96^{***}$). Results from this study show that GNDVI appeared very effective to estimate leaf nitrogen and grain protein content of rice canopy.

Anti-inflammatory Efficacy and Liver Protective Activity of Pine Pollen according to Probe Sonicator Ultrasonic Disintegration Extraction Method (송화분의 초음파 파쇄 추출 방법에 따른 항염증 효능 및 간 보호 활성)

  • Kim, Ok Ju;Woo, Young Min;Jo, Eun Sol;Jo, Min Young;Li, Chun-Ri;Lee, Young-Ho;Ahn, Mee Young;Lee, Sang-Hyeon;Ha, Jong Myung;Kim, Andre
    • Applied Chemistry for Engineering
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    • v.30 no.5
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    • pp.569-579
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    • 2019
  • In this study, the effect anti-oxidant, anti-inflammatory, and liver protective activity was investigated via quick ultrasonic disintegration of pine pollen using a probe sonicator (PS) followed by the extraction with water, 70% ethanol, and 100% ethanol. The anti-inflammatory effect was studied by measuring the production of nitric oxide (NO) and cytokine in RAW264.7 cells induced with lipopolysaccharides (LPS). The cell toxicity was also checked with an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the experiment was conducted using non-toxic $100{\mu}g/mL$. The NO inhibition rate was highest in the 70% ethanol PS group at $85.99{\pm}0.12%$. Also an excellent efficiency was obtained from the results of interlukin-1 beta ($IL-1{\beta}$) and tumor necrosis factor alpha ($TNF-{\alpha}$), which is related to inflammation-related cytokine, with the respective inhibition rates of 63 and 22%. To examine liver protective activity, HepG2 cells were treated with Taclin, and the generation of glutamic oxaloacetic transaminase (GOT) and lactate dehydrogenase (LDH) was measured in the culture solution. From GOT and LDH generation results, the inhibition rates in the 70% ethanol PS group were 28% and 13%, respectively, which was higher compared to that of using negative control group. Our results suggest that pine pollen extracted in 70% ethanol using PS may be used to develop food products that have anti-aging, anti-inflammatory, and liver protective effects.

Quality Characteristics and Antioxidant Activities of Morning Bread- Containing Aronia Sourdough Starter (아로니아 sourdough starter를 이용한 모닝빵의 품질특성 및 항산화 활성)

  • Sim, Sol;Park, Yeong-Ju;Lee, Jin-Ho;Jeong, So-Yeon;Lim, Ju-Jin;Yu, Ga-Hyun;Kim, Eun-Gyeom;Suh, Hee-Jae
    • Journal of Food Hygiene and Safety
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    • v.34 no.5
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    • pp.463-472
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    • 2019
  • This study investigated the quality characteristics and antioxidant activities of morning bread containing various amounts of aronia sourdough starter. Morning breads were prepared with different volumes (0% (AS0), 15% (AS1), 25% (AS2), and 35% (AS3)) of aronia sourdough starter based on wheat flour. In AS3 group (35% added group), the specific volume and baking loss rate were the highest but the bread height was the lowest. When the aronia sourdough starter was added up to 25%, the expansion power, specific volume, and bread height were significantly increased (P<0.05). According to the added amount of sourdough starters, the lightness and yellowness of the morning bread were decreased, however, redness was increased (P<0.05). In the rheology analysis, hardness, gumminess, and chewiness were significantly decreased with increasing amounts of aronia sourdough starters (P<0.05). However, cohesiveness was the highest in the AS2 group (25% added group). In consumer preference, the highest scores were shown in AS2 group (25% added group) in color, texture, and appearance. The total polyphenol and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging ability were both significantly increased along with aronia sourdough content (P<0.05). In conclusion, morning bread with 25% aronia sourdough starter showed the best quality characteristics and antioxidant activities.

Method Development for Determination of Chlorogenic Acid and Arbutin Contents in Fruits by UHPLC-MS/MS (UHPLC-MS/MS를 이용한 과일류 중 클로로젠산 및 알부틴 동시분석법 개발)

  • Choi, Young-Ju;Jeon, Jong-Sup;Kim, Woon-Ho;Jung, You-Jung;Ryu, Ji-Eun;Choi, Jong-Chul;Chae, Kyung-Suk;Lee, Jin-Hee;Do, Young-Sook;Park, Young-Bae;Yoon, Mi-Hye
    • Journal of Food Hygiene and Safety
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    • v.34 no.5
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    • pp.413-420
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    • 2019
  • In this study, a sample preparation method and a simultaneous determination method by ultra-high performance liquid chromatography coupled with tandem mass spectrometry for 9 isomers of chlorogenic acid and arbutin in fruits were developed. The samples were extracted using 90% methanol (pH 3.0), with the solutions being shaken and then sonicated for 10 min each. After centrifugation at 4,000 rpm for 10 min, the extraction was concentrated under a vacuum at $40^{\circ}C$ using a vacuum evaporator. The residue was dissolved in 5 mL of 5% methanol and filtered through a $0.45{\mu}m$ membrane before UHPLC-MS/MS analysis. The separations were performed on a C18 column with gradient elution of water (containing 0.1% formic acid) and methanol (containing 0.1% formic acid). The specificity, linearity, limit of detection, limit of quantification, accuracy, and precision of the proposed methods were also evaluated.

Analysis of Antioxidant Activity and Serotonin Derivatives in Safflower (Carthamus tinctorius L.) Germplasm Collected from Five Countries (국외 수집 홍화 유전자원의 항산화 활성 및 세로토닌 유도체 함량 분석)

  • Jung, Yi Jin;Assefa, Awraris Derbie;Lee, Jae Eun;Lee, Ho Sun;Rhee, Ju Hee;Sung, Jung Sook
    • Korean Journal of Plant Resources
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    • v.32 no.5
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    • pp.423-432
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    • 2019
  • In order to select potential plant resources as functional materials and natural antioxidants, we evaluated antioxidant activity and serotonin derivatives of safflower germplasm collected from five countries. N-(p-Coumaroyl) serotonin (CS) and N-feruloylserotonin (FS) were analyzed by using Ultra Performance Liquid Chromatography (UPLC). Total polyphenol content (TPC) was determined by Folin-Ciocalteu method and antioxidant activities were estimated by 2,2-diphenyl-1-picryl-hydrazil (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), Ferric reducing antioxidant power (FRAP) and Reducing power (RP) assays. The TPC showed a range of 28.25 to $90.53{\mu}g$ gallic acid equivalent (GAE)/mg dried extract (DE). DPPH, ABTS, FRAP and RP assay were in the range of 18.76 to 93.98, 48.91 to 163.73, 3.80 to 132.29 and 26.32 to $80.08{\mu}g$ ascorbic acid equivalent (ASCE)/mg DE, respectively. Among the five countries, safflower seed collected from Iran had the highest levels of serotonin derivatives and antioxidant activities than other countries (p<0.05). CS showed high correlation with TPC, ABTS and DPPH (r=0.673,0.727,0.820), and FS showed high correlation with DPPH (r=0.740). Accessions IT321214 and IT321215 could be useful for development of new functional materials and could be used as a source of valuable natural antioxidant materials.

Changes in Marine Environmental Factors and Phytoplankton Community Composition Observed via Short-Term Investigation in a Harbor in the Eastern Part of the South Sea of Korea (남해동부연안항만에서 하계 단주기 조사에 따른 해양환경 및 식물플랑크톤 군집조성의 변화)

  • Lee, Minji;Baek, Seung Ho
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.6
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    • pp.669-676
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    • 2017
  • To understand the relationship between environmental factors and phytoplankton community structures and why early outbreaks of Cochlodinium polykrikoides occur in the inner bay of Korea, short-term investigations were conducted at 17 stations in the eastern part of the South Sea of Korea, with sessions every two weeks from July 7 to August 24, 2016. The water temperature increased from $22.3^{\circ}C$ in the first survey to $28.4^{\circ}C$ in the fourth survey, which was a rise of about $6.01^{\circ}C$. Salinity was relatively high at Stns. 8 13 in the inner bay. In the first survey, rainfall of about 150 mm was observed, so nutrients were supplied at a high level and a high concentrate of Chl. a was observed. Cryptophyta (Crpytomonas spp.) represented 58.3 % of the community, followed by Bacillariophyta at 33.8 %. In particular, at Stn. 5, Dinophyta Prorocentrum spp. accounted for a very high percentage, 32.2 %. In the second survey, low phytoplankton populations were observed, and Bacillariophyta (Chaetoceros spp.) accounted for 61.0 %. At Stn. 4, Skeletonema spp. showed high populations but did not appear at other stations even at a low density. In the third and fourth surveys, phytoplankton populations were very low. Bacillariophyta represented 78.0 % in the third study and 73.3 % in the fourth. Interestingly, although the appearance of C. polykrikoides was investigated at the beginning of the red tide in the coastal area, they were not observed inshore, implying that the likelihood of inflow by the germination of resting cysts was low for the inner bay during this study period. In addition, environmental characteristics such as salinity and nutrient presence were significantly different between sampling stations due to the existence of a semi-closed bay in the southern sea, resulting in dominant phytoplankton species and community composition differing in these short-term investigations.