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http://dx.doi.org/10.7732/kjpr.2019.32.5.423

Analysis of Antioxidant Activity and Serotonin Derivatives in Safflower (Carthamus tinctorius L.) Germplasm Collected from Five Countries  

Jung, Yi Jin (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)
Assefa, Awraris Derbie (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)
Lee, Jae Eun (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)
Lee, Ho Sun (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)
Rhee, Ju Hee (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)
Sung, Jung Sook (Upland Crop Breeding, Research Division Department of Southern Area Crop Science, National Institute of Crop Science, RDA)
Publication Information
Korean Journal of Plant Resources / v.32, no.5, 2019 , pp. 423-432 More about this Journal
Abstract
In order to select potential plant resources as functional materials and natural antioxidants, we evaluated antioxidant activity and serotonin derivatives of safflower germplasm collected from five countries. N-(p-Coumaroyl) serotonin (CS) and N-feruloylserotonin (FS) were analyzed by using Ultra Performance Liquid Chromatography (UPLC). Total polyphenol content (TPC) was determined by Folin-Ciocalteu method and antioxidant activities were estimated by 2,2-diphenyl-1-picryl-hydrazil (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), Ferric reducing antioxidant power (FRAP) and Reducing power (RP) assays. The TPC showed a range of 28.25 to $90.53{\mu}g$ gallic acid equivalent (GAE)/mg dried extract (DE). DPPH, ABTS, FRAP and RP assay were in the range of 18.76 to 93.98, 48.91 to 163.73, 3.80 to 132.29 and 26.32 to $80.08{\mu}g$ ascorbic acid equivalent (ASCE)/mg DE, respectively. Among the five countries, safflower seed collected from Iran had the highest levels of serotonin derivatives and antioxidant activities than other countries (p<0.05). CS showed high correlation with TPC, ABTS and DPPH (r=0.673,0.727,0.820), and FS showed high correlation with DPPH (r=0.740). Accessions IT321214 and IT321215 could be useful for development of new functional materials and could be used as a source of valuable natural antioxidant materials.
Keywords
Antioxidants; Correlation; N-feruloylserotonin; N-(p-Coumaroyl) serotonin; Saffower seed;
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