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Production of Methane from Anaerobic Fermentation of Marine Macro-algae (해조류의 혐기성 발효를 이용한 메탄 생산)

  • Kim, Jeong-Min;Lee, Yeung-Ho;Jung, Sung-Hoon;Lee, Jin-Tae;Cho, Moo-Hwan
    • Clean Technology
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    • v.16 no.1
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    • pp.51-58
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    • 2010
  • Methane was produced from the anaerobic digestion of marine macro-algae. Elemental analysis was first performed to estimate the theoretical methane production of three macro-algae (Undaria pinnatifida, Laminaria japonica, Hizikia fusiformis). Three algae were found to contain C 34 ~ 36%, H 5%, O 37 ~ 43%, N 2 ~ 4%, S 0.4 ~ 0.7%, and ash 14~21%, and the theoretical methane content was in the range of 56 ~ 60%, which can produce 442 ~ 568 mL $CH_4$ per g of volatile solid (VS). Using the biological methane potential (BMP) test, we found that L. japonica resulted in the highest yield of methane (52%). Moreover, various operational conditions, such as algae amount, pH, salinity, particle size, and pre-treatment, were investigated in order to find an optimal condition of anaerobic digestion. At pH 8.0, the autoclaved L. japonica (5g VS/200 mL), when used without washing salt, produced 268.5 mL/g VS which is 65% of the theoretical methane productions. Furthermore, using a CSTR (with the working volume of 7 L out of the total volume of 10 L), we have successfully operated the reactor for 65 days and obtained maximum methane production rate of 1.4 L/day with purity of 70%.

Anaerobic Digestion of Distillery Wastewater in a Two-phase UASB System (이상 UASB 공정을 이용한 주정폐수의 혐기성소화)

  • Shin, Hang Sik;Bae, Byung Uk;Paik, Byung Cheon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.10 no.4
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    • pp.185-192
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    • 1990
  • A two-phase UASB system was operated for high-rate treatment of concentrated distillery wastewater. The phase separation was obtained by adjusting pH in each reactor. When influent SS concentration was 4.1/g/l, the first phase UASB reactor was effectively operated up to the loading rate of 16.5kg $COD/m^3.day$, producing 3.9g HAc/l.day. In the methanogenic UASB reactor, loading rate up to 44kg $COD/m^3.day$ could be applied while removing 80% of influent COD with a specific gas production of 16.5 l/l. day. After the formation granular sludge in both reactors, it was possible to maintain the appropriate pH in the first phase only by recirculating the effluent from methanogenic phase without the addition of alkaline chemicals.

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EQUATIONS AX = Y AND Ax = y IN ALGL

  • Jo, Young-Soo;Kang, Joo-Ho;Park, Dong-Wan
    • Journal of the Korean Mathematical Society
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    • v.43 no.2
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    • pp.399-411
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    • 2006
  • Let L be a subspace lattice on a Hilbert space H and X and Y be operators acting on a Hilbert space H. Let P be the projection onto $\frac\;{R(X)}$, where RX is the range of X. If PE = EP for each $E\;\in\;L$, then there exists an operator A in AlgL such that AX = Y if and only if $$sup\{{\parallel}E^{\bot}Yf{\parallel}/{\parallel}E^{\bot}Xf{\parallel}\;:\;f{\in}H,\; E{\in}L}=K\;<\;\infty$$ Moreover, if the necessary condition holds, then we may choose an operator A such that AX = Y and ${\parallel}A{\parallel} = K.$ Let x and y be vectors in H and let $P_x$ be the projection onto the singlely generated space by x. If $P_xE = EP_x$ for each $E\inL$, then the assertion that there exists an operator A in AlgL such that Ax = y is equivalent to the condition $$K_0\;:\;=\;sup\{{\parallel}E^{\bot}y{\parallel}/{\parallel}E^{\bot}x\;:\;E{\in}L}=<\;\infty$$ Moreover, we may choose an operator A such that ${\parallel}A{\parallel} = K_0$ whose norm is $K_0$ under this case.

Continuous Ethanol Fermentation Using Membrane Cell Recycle Fermentor (Membrane Cell Recycle Fermentor에 의한 에탄올 연속 발효)

  • 김태석;이석훈;손석민;권윤중;변유량
    • Microbiology and Biotechnology Letters
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    • v.19 no.4
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    • pp.419-427
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    • 1991
  • Ethanol fermentation of glucose by a strain of Saccharomyces cereuisiae was studied in membrane recycle bioreactor, where the fermentation vessel was coupled with cross flow hollow fiber membrane. The cell recycle system controlled backflushing with fresh medium was proven to be effective in alleviating membrane fouling and allowing long term operation of high-cell continuous fermentation. Using 100 g/l initial glucose concentration, the maximum productivity of about 9 5 g/$l \cdot h$ has been achieved at dilution rate 2.5 $h^{-1}$ and bleed stream ratio 0.05 with the corresponding ethanol concentration of 35g/l and glucose conversion of 100%. Increasing the glucose concentration to 200 g/$l \cdot h$ resulted in an increase in ethanol concentration to 48 g/l and productivity to 120 g1l.h. Substrate conversion, however, was only 69%. This productivity was the highest value in the study, and about 38 fold more than that of batch culture and 17 fold more that of single stage continuous culture without cell recycling. No further increase in the productivity was obtained when the glucose concentration was increased reased to 300g/l.

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A study on the vibration of 2-phase HB type Linear Stepping Motor (2상 HB형 선형 STEPPING 전동기의 진동해석에 관한 연구)

  • Oh, H.S.;Jung, E.N.;Sim, S.H.;Lim, Y.H.;Lee, S.K.;Shim, J.S.;Shim, N.S.;Lee, S.H.
    • Proceedings of the KIEE Conference
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    • 2001.07b
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    • pp.852-854
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    • 2001
  • In this paper, the vibration characteristics of a 2-phase Hybrid type Linear Stepping Motor(HLSM) are analyzed using the ACSL. A magnetic equivalent circuit is based on the structure of the HLSM, and then the electric equivalent circuit of the HLSM is derived by solving equations for the magnetic equivalent circuit. A normal force is calculated using FEM(Flux2D). And the vibration characteristics(Continuous vibration) of the HLSM are simulated by the ACSL with the voltage equations, the thrust equation, the normal force equation and the kinetic equation.

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Antagonism against Helicobacter Pylori and Proteolysis of Lactobacillus Helveticus CU631 and Strain Identification

  • Yoon, Y.H.;Won, B.R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.7
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    • pp.1057-1065
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    • 2002
  • The antagonistic activities of 30 strains of lactobacilli against Helicobacter pylori were determined and Lactobacillus helveticus CU631 has been selected as the strain which possesses the strongest inhibitory effect in the disc diffusion assay showing inhibition zone diameter of $10{\pm}1.5mm$, whereas those of L. plantarum and L. fermentum have been shown to be $4.0{\pm}0.6mm$. H. pylori G88016 revealed the highest vacuolating toxin producing activity among the 8 strains, the inhibitory activity of L. helveticus CU631 in vacuolating toxin producing activity of H. pylori manifested in the co-culture of two strains and in the 5:5 mixture of supernatant of the two strains. Both L. helveticus CU631 and cell free culture supernatant had a strong inhibitory activities in urease and cytotoxin producing activities of H. pylori NCTC11637 and CJH12. An accelerated proteolytic activity of water soluble peptides by L. helveticus CU631 during the refrigeration storage has been manifested in the cream cheese. DNA seqences of 16S-23S ribosomal RNA spacer region showed typical pattern among the various strains of L. helveticus, which could be used in the identification of L. helveticus CU 631.

Polysaccharide Production by Pseudomonas elodea ATCC 31461 (Pseudomonas elodea ATCC 31461에 의한 Polysaccharide 생산)

  • Yoo, Jin-Young;Shin, Dong-Hwa;Chung, Dong-Hyo
    • Korean Journal of Food Science and Technology
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    • v.19 no.5
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    • pp.414-419
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    • 1987
  • Cultural conditions for the polysaccharide production by Pseudomonas elodea ATCC 31461, a thermogellable polysaccharide producer, were investigated. Optimum condition for polysaccharide production were: temperature; $30^{\circ}C$, pH; 6.5, glucose; 25 g/L. nitrogen source; peptone, C/N ratio; 5.0. Maximum production and yield at this condition were ca. 32 g/L and 128%, viscosity of 1% polysaccharide solution was 837 mPas at 35/sec.. Consistency index and flow behaviour index were 2570 mPas and 0.66

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High Production of L-Threonine using Controlled Feeding of L-Methionine and Phosphate by Escherichia coli Mutant (L-Methionine과 Phosphate의 제한 공급에 의한 Escherichia coli MT201로부터의 고농도 L-Threonine 생산)

  • 이만효;이홍원;김병진;김천석;정준기;황용일
    • Microbiology and Biotechnology Letters
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    • v.32 no.2
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    • pp.149-153
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    • 2004
  • L-Threonine fermentation process was constructed on batch and fed-batch culture by using Escherichia coli MT201. The production type of L-threonine was observed as growth-associated production in batch culture. In fed-batch culture studying optimal concentration of yeast extract in feeding media, when 600 g/l of glucose and 60 g/l of yeast extract were added in feeding media, 87 g/$\ell$ of L-threonine was produced. To improve cell growth and L-threonine production, the culture of high cell density was performed in fed-batch culture with oxygen enriched air and feeding media containing L-methionine and phosphate. Under the conditions, we could achieve the highest L-threonine production of98 g/$\ell$ at 60 h. The highest productivity of L-threonine was about 3.85 g/$\ell$/h.

Biological Hydrogen Production from Mixed Organic Waste of Food and Activated Sludge by Pre-treatment (음식물쓰레기와 전처리한 폐활성슬러지의 혼합비율에 따른 생물학적 수소생산)

  • Lee, Jun-Cheol;Kim, Jae-Hyung;Choi, Kwang-Keun;Pak, Dae-Won
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.9
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    • pp.1044-1050
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    • 2007
  • In this study, Bio-hydrogen is produced from organic waste mixtures containing food waste and waste activated sludge (WAS). The effects of different operational factor on hydrogen production, including various solubilization methods for pretreatments of WAS, pH and different ratios of food waste and WAS, were investigated. The highest hydrogen production values are obtained as 4.3 mL $H_2/g$ $VS_{consumed}$ in the case of applying the mixed pre-treatments of alkali and ultrasonic. The pH value in bio-reactor increased from 4 to 8 after the ultrasonic treatment with alkali and the hydrogen yield touched its highest value in the pH range of 5.0 to 5.5. Similarly, the hydrogen production reached the level of 13.8 mL $H_2/g$ $VS_{consumed}$ using the same pre-treatment method from the mixture of food waste and WAS. The ratio of 2 : 1 produced a maximum amount of hydrogen of 5.0 L $H_2/L/d$. The amount of volatile fatty acids(VFAs) including acetate, propionate and butyrate, were also varied considerably. Propionate decreased consistently with rising of hydrogen while butyrate comparing to acetate relatively increased in the effluent.

Studies on Purification of Mine Drainage with NaOCl and $H_2O_2$ (산화제 NaOCl와 $H_2O_2$를 이용한 광산배수 정화에 관한 연구)

  • Choi, Seung-Won;Jang, Yun-Deug;Kim, Young-Hun;Kim, Jeong-Jin
    • Economic and Environmental Geology
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    • v.43 no.1
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    • pp.21-31
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    • 2010
  • Mine and leachate waters were collected from the Okdong mine for study on reaction with oxidizing agents such as NaOCl and $H_2O_2$. The pH and EC of the mine and leachate waters are 5.77, 831 uS/cm, and 6.38, 1920 uS/cm, respectively. The concentrations of Mg, Mn, and Zn are 23.25 mg/l, 14.90 mg/l, and 22.99 mg/l for the mine water and 98.75 mg/l, 3.38 mg/l, and 6.16 mg/l for the leachate water. The concentrations of Mg, Mn and Zn decreased after the reaction with the oxidizing agents and mine water. The concentrations of Mg, Mn and Zn rapidly decreased when oxidizing agents increased. The saturation indices that were computed by visual MINTEQ for initial mine and leachate water were undersaturated with Mg, Zn and Mn compounds. The precipitates after the reaction with the oxidizing agents are composed mainly of mangano-calcite[(Mn, Ca)$CO_3$] with small amount of calcite-magnesian and calcite.