• Journal of Plant Biotechnology
• /
• v.34 no.3
• /
• pp.207-212
• /
• 2007

This study describes a simple and rapid method for quantitative determination of $\gamma$-aminobutyric acid (GABA) using $^1H$ NMR spectroscopy from whole cell extracts of plant suspension cultures. When 9 cell lines derived from 8 species of higher plants maintained in liquid Marashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) were subjected to $^1H$ NMR, a cell line of Coriandrum sativum L. exhibited the highest level of GABA. The level reached up to 16.9 mg/dry wt when cells were cultured in MS medium supplemented with 0.5 mg/L 2,4-D after 3 weeks of incubation. The method for quantitative determination of GABA using $^1H$ NMR established in this study could be applied to high-throughput screening of various plant resources for GABA production and the cell suspension culture system of C. sativum could be further developed for commercial production of GABA.

• Journal of Korean Society for Atmospheric Environment
• /
• v.29 no.6
• /
• pp.811-817
• /
• 2013

A two-stage bioreactor system using sulfur-oxidizing bacteria was studied to abate high strength hydrogen sulfide ($H_2S$) from biogas. The two-stage bioreactor consisted of a $H_2S$ absorption column (0.5 L) and a microbial oxidation column (1 L) in series, and the liquid medium was continuously recirculated through the columns. The objectives of this study were to determine the feasibility of the bioreactor for biogas desulfurization and to investigate the effect of the medium circulation rate on the system performance. An averaged concentration of $H_2S$ introduced to the bioreactor was 530 ppm, corresponding to an overall loading rate of $44.4g/m^3/hr$. During the initial 20 days period at the medium recirculation rate of 8 reactor volumes per hour (12 L/hr), the dissolved oxygen (DO) concentration in the oxidation column was 6 mg/L, while the DO in the absorption column was 0.5 mg/L showing that the oxygen contents of the biogas stream was not altered. Because of the biological oxidation of $H_2S$ in the oxidation column, the sulfate concentration increased from 200 mg/L to 5,600 mg/L in the liquid medium. The removal efficiency of $H_2S$ was greater than 99% in the initial operation period. After the initial period, the medium recirculation rate between the two columns was stepwise changed eight times from 1.0 to 40 vol/hr (1.5~60 L/hr). At the recirculation rate of faster than 4 vol/hr, the $H_2S$ removal efficiencies were found to be high, but the efficiency declined at the lower recirculation rates than the threshold.

• Environmental Engineering Research
• /
• v.20 no.2
• /
• pp.175-180
• /
• 2015

This research focused on the solid and nitrate removal efficiency in a solid digestion-denitrification column. The 20 L up-flow column consisted of 18 L acrylic column with 2 L down-comer inlet tube located in the middle. In the first part, the wastewater with high suspended solids from the Tilapia fish tank was applied into the sedimentation unit at 5 variable flow rates i.e., 11.25, 25.71, 60, 105.88 and 360 L/h. The results indicated that the flow rate of 11.25 L/h (0.57 m/h) gave the highest solid removal efficiency of $72.72%{\pm}8.24%$. However, the total suspended solids removal was highest at 360 L/h (18.13 m/h). In the second part, methanol was added as an external organic carbon source for denitrification process in a hybrid column containing settled solids. The COD:N ratios of 0.5:1, 1:1, 2:1, 3:1, 4:1, 5:1 and 6:1 were investigated and compared with control without methanol addition. This experiment was operated at the HRT of 1 h with 450 L wastewater from recirculating aquaculture pond containing 100 mg-N/L sodium nitrate. The results indicated that the COD:N ratio of 3:1 gave the highest nitrate removal efficiency of $33.32%{\pm}21.18%$ with the denitrification rate of 5,102.88 mg-N/day.

• Journal of Plant Biotechnology
• /
• v.46 no.1
• /
• pp.9-16
• /
• 2019

Dendrobium candidum Wallich ex Lindley is a traditional Chinese medicine plant and has been widely used for medicinal and ornamental purposes. In this study, several different factors affecting micro propagation of protocorm-like bodies (PLBs) such as basal media, plant growth regulators, and carbon sources. The proliferation PLB derived from seeds was the best in $H_3P_4$ basal medium containing $0.1mg{\cdot}L^{-1}$ NAA and $0.1mg{\cdot}L^{-1}$ Kinetin. PLB growth was the best when $10g{\cdot}L^{-1}$ sucrose was added to the carbon atoms in the medium. The rate of shoot formation from the propagated PLB was the highest in 1/4 MS or $H_1P_2$ medium containing $10g{\cdot}L^{-1}$ sucrose, and the shoot length was longer than the others.

• Korean Journal of Plant Resources
• /
• v.25 no.4
• /
• pp.456-464
• /
• 2012

This study was conducted to establish the in vitro optimal condition for seed germination and organogenesis of wild Angelica gigas. The experiment was evaluated the effects of $GA_3$ for pre-treatment with different periods of time (0h, 24h, 48h, 72h) and followed the treatment of seeds by control, scarification and methanol-heating method. As a result, the highest rates (15%) of seed germination was shown under the treatment without soaking of $GA_3$ and methanol-heating treatment. The seed germination was highly increased 60% under the condition of treatment on ultrasonic waves (frequency 80 KHz) with methanol-heating treatment including 0.1 mg/L $GA_3$. The highest callus induction rate was obtained from in vitro germinated stem, root and hypocotyl on the MS medium with 1.0 mg/L NAA and 0.5 mg/L BA. The highest percentages of shooting (50%) and rooting (85%) induction were observed in hypocotyl and root cultured on PGRs free medium and 0.1 mg/L NAA, respectively. In addition, somatic embryogenesis was observed from stem (1.0 mg/L 2,4-D) and hypocotyl (0.1 mg/L NAA).

• Broadcasting and Media Magazine
• /
• v.7 no.3
• /
• pp.19-23
• /
• 2002

본 장에서는 H.26L 비디오 코덱에 적용되는 움직임 보상 기법(motion compensation) 및 B 픽쳐(Bi-predictive picture) 에 대해 다루기로 한다. 먼저 H.26L 비디오 코덱이 H.263 또는 MPEG-4 Part 2와 같은 종래 비디오 코덱들과 움직임 보상 관점에서 다른 점을 살펴보면, 복수개의 레퍼런스 픽쳐(multiple reference picture) 로부터 움직임 추정 (motion estimation) 밀 움직임 보상을 수행하고, 16x16 매크로 블록 크기부터 16x8, 8x16, 8x8블록 크기에서 매크로 블록 모드 타입이 결정되고 8x8 모드는 다시 8x4, 4x8, 4x4 단위에서 서브 모드 타입이 결정된다. 따라서 한 개의 매크로블록은 최대 16개의 모션벡터를 깆을 수 있다. 또한 복수개의 레퍼런스 픽쳐와 다양한 블록 타입을 적용함에 따라 모션벡터 예측(PMV: prediction of motion vector) 은 현재 블록과 이웃하는 블록들 사이의 레퍼런스 픽쳐 인덱스 비교 및 현재 블록 타입이 16x8또는 8x16 일 때 방향성 예측을 허용하는 새로운 기법들이 소개되어 있다. 그리고 코딩 효율을 높이기 위한 방법으로서 1/4 pel 단위의 움직임 보상을 하여 블록의 예측 정확도를 높이도록 하고 있다. 한편, H.26L에서의 B 픽쳐는 종래 비디오 코덱에 비해 확장된 정의를 갖고 있다. 예를 들어, 종래의 비디오 코덱에서는 B 픽쳐가 시간 스케일러빌러티(temporal scalability)에 사용됨에 따라 레퍼런스 픽쳐로서 사용될 수 없지만, H.26L은 B 픽쳐가 레퍼런스 픽쳐로서 사용되는 것을 허용하고 있다. 또한 종래의 B 픽쳐가 양방향에서 움직임 보상을 한 것과 달리 H.26L은 동일 방향에 존재하는 두개의 레퍼런스 픽쳐에서도 움직임 보상을 허용한다.

• Archives of Pharmacal Research
• /
• v.29 no.10
• /
• pp.859-865
• /
• 2006

With an approach to study the anti-tumor effects and mechanism of selenium compound, we investigated the anti-tumor activity and mechanism of $Na_5SeV_5O_{18}{\cdot}3H_2O$ (NaSeVO) in K562 cells. The results showed that $0.625{\sim}20\;mg/L$ NaSeVO could significantly inhibit the proliferation of K562 cells in vitro in a time- and concentration-dependent manner as determined by microculture tetrazolium (MTT) assay, the IC50 values were 14.41 (4.45-46.60) and 3.45 (2.29-5.22) mg/L after 48 hand 72 h treatment with NaSeVO respectively. In vivo experiments demonstrated that i.p. administration of 5, 10 mg/kg NaSeVO exhibited an significant inhibitory effect on the growth of transplantation tumor sarcoma 180 (S180) and hepatoma 22 (H22) in mice, with inhibition rate 26.8% and 58.4% on S180 and 31.3% and 47.4% on H22, respectively. Cell cycle studies indicated that the proportion of G0/G1 phase was increased at 2.5 mg/L while decreased at 10 mg/L after treatment for 24, 48 h. Whereas S phase was decreased at 2.5-5 mg/L and markedly increased at 10 mg/L after treatment for 48 h. After treatment for 24 h, 10 mg/L NaSeVO also markedly increased S and G2/M phases. Take together, the result clearly showed that NaSeVO markedly increased S and G2/M phases at 10 mg/L. The study of immunocytochemistry showed that the expression bcl-2 is significantly inhibited by 10 mg/L NaSeVO, and bax increased. Morphology observation also revealed typical apoptotic features. NaSeVO also significantly caused the accumulation of $Ca^{2+}$ and $Mg^{2+}$, reactive oxygen species (ROS) and the reduction of pH value and mitochondrial membrane potential in K562 cells as compared with control by confocal laser scanning microscope. These results suggest that NaSeVO has anti-tumor effects and its mechanism is attributed partially to apoptosis induced by the elevation of intracellular $Ca^{2+}$, $Mg^{2+}$ and ROS concentration, and a reduction of pH value and mitochondria membrane potential (MMP).

• Journal of the Korean Institute of Telematics and Electronics A
• /
• v.31A no.8
• /
• pp.72-79
• /
• 1994

In this paper semi empirical models are presented for the hot electron induced threshold voltage shift(${\Delta}V_{t}$) and effective channel shortening length (${\Delta}L_{H}$) in degraded PMOSFET. Trapped electron charges in gate oxide are calculated from the well known gate current model and ΔLS1HT is calculated by using trapped electron charges. (${\Delta}L_{H}$) is a function of gate stress voltage such as threshold voltage shift and degradation of drain current. From the correlation between (${\Delta}L_{H}$) has a logarithmic function of stress time. From the measured results, (${\Delta}V_{t}$) and (${\Delta}L_{H}$) are function of initial gate current and device channel length.

• Journal of Korean Society of Environmental Engineers
• /
• v.31 no.11
• /
• pp.983-988
• /
• 2009

This study was performed to evaluate the toxicity and seek the control method of the wastewater in which nickel (Ni) was included into an industrial wastewater treatment plant. Nickel concentration of the wastewater, of which samples were taken every hour during 24hours, were various from 0.33 to 116.0 mg/L, with 24.0 mg/L of the average concentration. IC50 values against nitrosomonas and nitorbactor, a toxic level against bacteria which could inhibit 50% of nitrification bacteria in the wastewater, are 5.5 and 4.9 mg/L respectively. Nickel in this industrial wastewater can inhibit the 50% of nitrification bacteria even after diluting this wastewater 5 times. Also, this research, which reduced the nickel concentraion, forming nickel hydroxide compounds by increasing pH of the wastewater, shows that nickel concentraion can be obtained under 1.7 mg/L at pH 11 and 0.6 mg/L at pH 12. Consequently, the result of this study is that the nitrification efficiencies can be obtained from 83.8 to 99.4% with 97.6% of the average in the biological treatment after removing nickel in the wastewater by increasing the pH above 11~12, which is forming the nickel hydroxide compounds.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.30 no.1
• /
• pp.1-7
• /
• 1997

In order to utilize fish by-products from the skipjack tuna (Katsuwonus pelamis) canning manufactures Lactobacillus buigaricus KCTC 3188 and L. piantarum KCTC 1048 were used as a starter culture for the preparation of fermented fish silage with skipjark tuna viscera. The optimum temperature and pH on barterial growth and lactic acid production of L. bulgaricus and L. plantarum in MRS broth were $35^{\circ}C$ and around pH 6.0, respectively. And the optimum concentrations of the carbohydrate sources added to the broths were $7\%$ for dextrose and $10\%$ for molasses on the basis of total weights of skipjack tuna viscera. The pH of acid treated skipjack tuna viscera silage (ASS) slightly increased from 4.0 to 4.5, while that of fermented skipjack tuna viscera silages by the use of lactic acid bacterias (FSS) was significantly declined from 5.9 to about 40 after 42 days of storage at $35^{\circ}C$. Though the content of volatile basie nitrogen (VBN) in ASS was lower than those of FSS after 42 days of storage at $35^{\circ}C$, VBN content in silages slightly increased from an initial value of $62\~65{\cdot}mg/100g$ to final value of $113\~155\;mg/100g$ over 42 days. The fermented silage by L. piantarum reached a maximum concentration of amino nitrogen and showed $81\%$ of hydrolysis degree after 4 days of storage at $35^{\circ}C$.