Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Establishment of a Simple and Rapid Method for Quantitative Determination of -Aminobutyric Acid Using 1H NMR and Production of γ-Aminobutyric Acid in Cell Suspension Cultures of Coriandrum sativum L.

1H NMR에 의한 γ-Aminobutyric Acid의 간단하고 신속한 정량분석법 확립과 고수 (Coriandrum sativum L.) 현탁배양세포로부터 γ-Aminobutyric Acid의 생산

  • Kim, Suk-Weon (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Liu, Jang-R. (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 김석원 (한국생명공학연구원, 생물자원센터) ;
  • 유장렬 (한국생명공학연구원, 식물유전체연구센터)
  • Published : 2007.09.29
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Abstract

This study describes a simple and rapid method for quantitative determination of $\gamma$-aminobutyric acid (GABA) using $^1H$ NMR spectroscopy from whole cell extracts of plant suspension cultures. When 9 cell lines derived from 8 species of higher plants maintained in liquid Marashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) were subjected to $^1H$ NMR, a cell line of Coriandrum sativum L. exhibited the highest level of GABA. The level reached up to 16.9 mg/dry wt when cells were cultured in MS medium supplemented with 0.5 mg/L 2,4-D after 3 weeks of incubation. The method for quantitative determination of GABA using $^1H$ NMR established in this study could be applied to high-throughput screening of various plant resources for GABA production and the cell suspension culture system of C. sativum could be further developed for commercial production of GABA.

식물현탁배양세포의 whole cell extract의 $^1H$ NMR 스펙트럼 데이터로부터 통계분석기법을 활용하여 GABA의 간단하고 신속한 정량분석방법을 확립하였다. 이 기술을 활용하여 고등식물 8종의 9개 세포주를 MS 배지에 1 mg/L의 2,4-D를 첨가한 배지에 유지하였을 때 고수 (Coriandrum sativum L.)가 가장 많은 양의 GABA를 생산하였다. 고수 현탁배양세포로부터 2,4-D농도 및 배양기간에 따른 GABA의 생산성 변화를 조사한 결과 현탁배양세포를 0.5 mg/L 2,4-D가 첨가된 배지에서 3주간 배양된 현탁배양세포를 이용할 경우 GABA 함량이 건중량 1 g 당 16.9 mg으로 가장 높게 생산되었다. 본 연구에서 확립된 간단하고 신속한 분석법으로 다양한 식물자원으로부터 GABA의 생산성을 초고속탐색(high-throughput screenig)할 수 있을 것이며 고수 현탁세포배양법으로 GABA의 상업적 대량생산이 가능할 것으로 전망된다.

Keywords

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