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http://dx.doi.org/10.7732/kjpr.2012.25.4.456

Effects of Plant Growth Regulators on In Vitro Germination and Organ Formation of Wild Angelica gigas N.  

Lee, Su-Gwang (Department of Biological and Environmental Science, Dongguk University)
Cho, Won-Woo (Department of Biological and Environmental Science, Dongguk University)
Lee, Song-Hee (Department of Biological and Environmental Science, Dongguk University)
Park, Kwang-Woo (Korea National Arboretum)
Choi, Kyung (Korea National Arboretum)
Kang, Ho-Duck (Department of Biological and Environmental Science, Dongguk University)
Publication Information
Korean Journal of Plant Resources / v.25, no.4, 2012 , pp. 456-464 More about this Journal
Abstract
This study was conducted to establish the in vitro optimal condition for seed germination and organogenesis of wild Angelica gigas. The experiment was evaluated the effects of $GA_3$ for pre-treatment with different periods of time (0h, 24h, 48h, 72h) and followed the treatment of seeds by control, scarification and methanol-heating method. As a result, the highest rates (15%) of seed germination was shown under the treatment without soaking of $GA_3$ and methanol-heating treatment. The seed germination was highly increased 60% under the condition of treatment on ultrasonic waves (frequency 80 KHz) with methanol-heating treatment including 0.1 mg/L $GA_3$. The highest callus induction rate was obtained from in vitro germinated stem, root and hypocotyl on the MS medium with 1.0 mg/L NAA and 0.5 mg/L BA. The highest percentages of shooting (50%) and rooting (85%) induction were observed in hypocotyl and root cultured on PGRs free medium and 0.1 mg/L NAA, respectively. In addition, somatic embryogenesis was observed from stem (1.0 mg/L 2,4-D) and hypocotyl (0.1 mg/L NAA).
Keywords
Angelica gigas; In vitro germination; Micropropagation; Somatic embryogenesis;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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