[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5010/JPB.2007.34.3.207

Establishment of a Simple and Rapid Method for Quantitative Determination of -Aminobutyric Acid Using ¹H NMR and Production of γ-Aminobutyric Acid in Cell Suspension Cultures of Coriandrum sativum L.

Kim, Suk-Weon (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Liu, Jang-R. (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))

Publication Information

Journal of Plant Biotechnology / v.34, no.3, 2007 , pp. 207-212 More about this Journal

Abstract

This study describes a simple and rapid method for quantitative determination of $\gamma$ -aminobutyric acid (GABA) using $^1H$ NMR spectroscopy from whole cell extracts of plant suspension cultures. When 9 cell lines derived from 8 species of higher plants maintained in liquid Marashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) were subjected to $^1H$ NMR, a cell line of Coriandrum sativum L. exhibited the highest level of GABA. The level reached up to 16.9 mg/dry wt when cells were cultured in MS medium supplemented with 0.5 mg/L 2,4-D after 3 weeks of incubation. The method for quantitative determination of GABA using $^1H$ NMR established in this study could be applied to high-throughput screening of various plant resources for GABA production and the cell suspension culture system of C. sativum could be further developed for commercial production of GABA.

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Times Cited By KSCI : 1 (Citation Analysis)

Reference
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