• Archives of Pharmacal Research
• /
• 제27권5호
• /
• pp.570-575
• /
• 2004

2-Hydroxymuconic semialdehyde (2-HMS) dehydrogenase catalyzes the conversion of 2-HMS to 4-oxalocrotonate, which is a step in the meta cleavage pathway of aromatic hydrocarbons in bacteria. A tomC gene that encodes 2-HMS dehydrogenase of Burkholderia cepacia G4, a soil bacterium that can grow on toluene, cresol, phenol, or benzene, was overexpressed into E. coli HB 101, and its gene product was characterized in this study. 2-HMS dehydrogenase from B. cepacia G4 has a high catalytic efficiency in terms of V$_{max}$K$_{max}$ towards 2-hydroxy-5-methyl-muconic semialdehyde followed by 2-HMS but has a very low efficiency for 5-chloro-2-hydroxymuconic semialdehyde. However, the enzyme did not utilize 2-hydroxy-6-oxo-hepta 2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid as substrates. The molecular weight of 2-HMS dehydrogenase from B. cepacia G4 was predicted to be 52 kDa containing 485 amino acid residues from the nucleotide sequence of the tomC gene, and it exhibited the highest identity of 78% with the amino acid sequence of 2-HMS dehydrogenase that is encoded in the aphC gene of Comamonas testosteroni TA441. 2-HMS dehydrogenase from B. cepacia G4 showed a significant phylogenetic relationship not only with other 2-HMS dehydrogenases, but also with different dehydrogenases from evolutionarily distant organisms.sms.

• Archives of Pharmacal Research
• /
• 제23권2호
• /
• pp.187-195
• /
• 2000

Nucleotide sequence extending 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (pcbC) and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase gene (pcbD) of Pseudomonas sp. DJ-12 was previously analyzed and the two genes were present in the order of pcbD-pcbC preceded by a promoter from Pseudomonas sp. DJ-12. In this study, a 3.8-kb nucleotide sequence located downstream of the pcbC gene was analyzed to have three open reading frames (ORFs) that are designated as orf1, pcbE and orf2 genes. All of the ORFs were preceded by each ribosome-binding sequence of 5-GGAXA-3 (X=G or A). However, no promoter-like sequence and transcription terminator sequence were found in the analyzed region, downstream of pcbC gene. Therefore, the gene cluster appeared to be present in the order of pcbD-pcbC-orf1-pcbE-orf2 as an operon, which is unique organization characterized so far in biphenyl- and PCB-degrading bacteria. The orf1 gene was composed of 1,224 base pairs which can encode a polypeptide of molecular weight 44,950 containing 405 amino acid residues. A deduced amino acid sequence of the orf1 gene product exhibited 21-33％ identity with those of indole dioxygenase and phenol hydroxylase components. The pcbE gene was composed of 783 base pairs encoding 2-hydroxypenta-2,4-dienoate hydratase involved in the 4-chlorobiphenyl catabolism. The orf2 gene was composed of 1,017 base pairs encoding a polypeptide of molecular weight 37,378 containing 338 amino acid residues. A deduced amino acid sequence of the orf2 gene product exhibited 31％ identity with that of a nitrilotriacetate monooxygenase component.

• 한국전자파학회논문지
• /
• 제30권5호
• /
• pp.407-417
• /
• 2019

탄도 미사일은 상공에서 자유 낙하하며 표적을 요격하기 때문에 탄도 미사일에 부착되는 탐색기는 높은 지표각에서 해상 클러터 영향을 받게 되며, 그 결과 탐색기의 탐지 성능이 급격히 낮아지게 된다. 이를 해결하기 위해서는 다양한 시나리오 기반의 시뮬레이션들을 통한 해상 표적 탐지 성능 분석이 반드시 필요하다. 따라서 본 논문에서는 실제와 유사한 높은 지표각의 해상 클러터 수신 신호를 모델링한 후, 이를 신호 대 클러터 비에 따라 해상 표적 수신 신호와 합성하여 2차원 레이다 영상을 생성한다. 이후, 레이다 영상에 2차원 CA-CFAR 탐지기를 적용하여 다양한 시나리오에서 해상표적 탐지 성능을 분석하였다. CAD 모델과 전자기 수치해석 도구를 사용한 시뮬레이션 결과, 지표각과 방위각에 따라 해상 표적의 탐지 여부가 다르게 나타남을 확인할 수 있었다.

• 보건의료산업학회지
• /
• 제12권2호
• /
• pp.63-73
• /
• 2018

Objectives : This study aimed investigate general characteristics, vaccination status and health beliefs related to vaccination for herpes zoster and to identify variables. Methods : The structured questionnaire was conducted from March 31, 2016 to April 20, 2016 with 312 outpatients who visited a university hospital. The data were analyzed by frequency analysis, t-test, $x^2-test$, correlation analysis and logistic regression analysis via SPSS statistics 22.0. Results : The vaccination rate of the herpes zoster was 16.0% and the vaccination cost was the most common reason for rejecting inoculation. More highly educated people with more than a university degree(OR=4.28, p=.002), those with higher susceptibility to herpes zoster(OR=1.56, p=.000), those more aware of the benefits of vaccination(OR=1.51, p=.009) and those with lower disability for vaccination(OR=0.74, p=.003) were more likely to be vaccinated. Conclusions : Therefore, to increase the vaccination rate of herpes zoster in adults over 50 years old, continuous publicity and education are needed to emphasize the susceptibility of herpes zoster and the benefit of vaccination. In addition, it is necessary to develop policies at the national level for the expensive vaccination cost that obstructs vaccination.

• 대한한방내과학회지
• /
• 제12권2호
• /
• pp.113-128
• /
• 1991

This study has been carried out to investigate Pyu-Juk ( 肺積 ), Pyu-Ong ( 肺癰 ), Pyu-Ju ( 肺疽 ), Pyu-Am ( 肺癌 ) by referring to 56 literatures. The results were obtained as follows; 1. The treatllent-method of Pyu-Juk ( 肺積 ) are Ik Ki ( 益氣 ), SuI Yem Wha ( 洩陰火 ), So Juk ( 消積 ), Wha Juk ( 化積 ), Son Juk ( 損積 ), Ma Juk ( 摩積 ) Jo Chil Gi ( 調七氣 ), and herbs are Sik Bun Tang ( 息賁湯 ), Sik Bun Hwan ( 息賁丸 ), O Juk Hwan ( 五積丸 ), Ban ha Tang ( 半夏湯 ), Gil Gyung Tang ( 桔梗湯 ), Dae Chil Gi Tang ( 大七氣湯 ), Chil Gi Hwan ( 七氣湯 ) , Ga Gam Sik BlDl Hwan ( 加減息賁丸 ), Bil Rang San ( 檳郞散 ). 2. The treatment-method of Pyu-Ong ( 肺癰 ) Yang pyu ( 養肺 ), Yang Hyul ( 養血 ), Yang Gi ( 陽氣 ), Chung Geam Youn Pye ( 淸金潤肺 ), Hal Dam Bae Nong ( 豁痰排膿 ), Saeng Gi ( 生肌 ), herbs are Gil Gyung Tang ( 桔梗湯 ), Jung Ryuk Dae Jo Sa Pyu Tang ( ??大棗瀉肺湯 ), Chung Geom Wy Gyung Tang ( 千金 葦莖湯 ) Hwang Gi tang ( 黃嗜湯 ), Hyun Sam Chung Pyu Eum ( 玄蔘淸肺飮 ), Sip Mi Hwan ( 十味丸 ), Gil Gyung Baek San ( 桔梗白散 ), So Hong Eum ( 消膿飮 ), Sam Gi Bo Pyu Tang ( 蔘嗜補肺湯 ), sam Chul Gun Bi Tang ( 蔘朮健脾湯 ), Chung Pyu Gil Gyung Tang ( 淸肺桔梗湯 ), Yu Sung Hwan ( 如聖丸 ). 3. The treatment-method of Pyu-Ju ( 肺疽 ) are Be Bi ( 補脾 ), Bo Pyu ( 補肺 ), herbs are Hwang Gi Gum Jung Tang( 黃嗜補裨湯 ), lnSamBoPyuSan (人蔘補肺散) 4. The treatment-method of Pyu-Am ( 肺癌 ) are Bal Han ( 發汗 ), Chung Yul Hae Dok ( 淸熱解毒 ), Gang Hwa Do Dam ( 降火導痰 ), Hwa Rak Hwa a ( 和絡化瘀 ) Ik Pyu ( 益肺 ), Gun Bi ( 健脾 ), Ja Eum Yun Pyu ( 滋陰潤肺 ), Gi Hu Yang Bo ( 氣虛兩補 ), herbs are Gyul Yua Hae Dok Tang ( 莖熱解毒湯 ), Gang Hwa Jae ( 降火劑 ), Chil Yul Do Dam Tang ( 治熱導痰湯 ), Chong Ho Byul Gap Tang ( 靑蒿鱉甲湯 ), Ga gam Gil Gyung Tang ( 加減桔梗湯 ), Sang Mak San ( 生脈散), Yuk Mi Ji Hwang Tang ( 六味地黃湯 ), Baek Hap Go Geum Tang ( 百合固金湯 ), Dae Be Won Jun ( 大補元煎 ), Ga Mi Jae Che Bo Pyu Tang ( 加味載體補肺湯 ).

• 동국한의학연구소논문집
• /
• 제2권1호
• /
• pp.73-105
• /
• 1993

To say nothing of the orient and the west, the human beings discover the method of Jin Mac(診脈) by the way that observe disease. But oriental medicine devise special method of Jin Mac(診脈) in the study of Kyoung Mac(經脈). Although sip-ei Kyoung Mac Jin(十二經脈診), Sam Bu Gu Who Jin(三部九候診), In Young Mac Gu Jin(人迎脈口診), Chon Kwan Chuck Jin(寸關尺診) namely Yuk Bu Jung Wee Jin Mac (六部定位診脈) that is used today are devised, it has changed naturally by the changing treatment and the introduction of Yuin Yang(陰陽) and five element(五行). Many methods dg Jin Mac(診脈), it had not developped successing alternative, it had developped of declined by it's own way. 1. Results for the birth of Mac(脈) 1) Mac(脈), it means Kyoung Mac(經脈), at first entirelly Mac(脈), is seized a blood vessel that flows in the body. As presumed today, after finding many acupunture point, a general idea of Mac(脈) is not maked by the line that connect point and point, it connect between acupunture point and acupunture point. 2) Like blood flows in Hyul Mac(血脈), Gie(氣) flows in Kyoung Mac(經脈). The two things relate deeply each other. In a general idea or actrally Kyoung Rak(經洛), the two things sometimes accord, sometimes seperate, sometimes mix alternative. 3) Hyul Mac(血脈) and Kyoung Mac(經脈), we call it Mac(脈) entirely Kyoung Mac(經脈), is a way that manifest disease through Kyoung Mac(經脈) or a boundary that disease belongs to it method of Mac Jin(脈診) individual that disease of Kyoung Mac(經脈) is diagnosed by the jumping situation of Hyul Mac(血脈). 4) In method of Moxa, athough the pathology and the diagnostic of Mac(脈) are created by finding Mac(脈). Finding acupunture have opportunitty fot Mac Jin(脈診) and treatment. 2. Results of Kyoung Mac Mac Jin(經脈脈診) 1) In theory of kyoung Rak(經洛), disease are resumed for malfunction of Young Wee(榮衛) that flows in Kyung Rak(經洛). So to speak, in treatment of Kyoung Rak area, the purpose of diagnosis observe the situation of disease and cause. For fitting the purpose of diagnosis, the dead had esatablised four-diagnosis method mangMunMnnJeul(望聞問切), in four-diagnosis(四診法), the core is Mac Jin(脈診). 2) sip-ei Kyoung Mac Mac Jin(十二經脈診) had existed as Kyoung Mac Mac Jin(經脈脈診), it precedes Sam Bu Gu Who Jin(三部九候診). In Young Ki Gu Mac(人迎脈口診). 3) Although Bu Yang Mac(趺陽脈), So Um Mac(少陰脈) is a part of Sip-ei Kyoung Mac(十二經脈診), they developped especially because they located in the point of Won Hyul(原穴) and they are convenient for diagnose. 4) Sip-ei Kyoung Mac Mac Jin(十二經脈診), which belongs to Bu Yang Mac(趺陽脈) and So Urn Mac(少陰脈), is not important for the comming age medical books compared with Mac Kyoung(脈經). 3. Results gor Sam Bu Gu Who Jin(三部九候診) 1) Mac Jin(脈診) of Sam Bu Gu Who(三部九候), which is noted in the theory of Sam Bu Gu Who(三部九候診) of So Mun(素問), belongs to Kyoung Mac Mac Jin's(經脈脈診) geneology, Sip-ei Kyoung Mac Mac Jin(十二經脈診) is arranged, simplicated by the idealogy three talents(三才思想) in the heaven and the earth. 2) What Sam Bu Gu Jin(三部九候診) is regardded as very important in So Mun(素問), the editor of So Mun(素問) recognize the meaning that one discover disease early in this method of diagnosis. 3) After Young chu(靈樞), Nan Kyoung(難經) it is lacked the method of Sam Bu Gu Who Jin(三部九候診) in the books that treatment has changed. Sam Bu Gu Who Jin(三部九候診) based on actually clinic appropriate. 4. results for In Young Mac Gu Jin(人迎脈口診) 1) In Young Mac Gu Jin(人迎脈口診) is the method of comparative Mac Jin(脈診) according to the theory of Yin Yang(陰陽), it is presumed after Sam Bu Gu Who Jin(三部九候診), it had perished in parallel with the development of the theory of five elelment(五行). The development of the acupunture, the perishment of the treatment of negative(刺絡). 2) In Young Mac Gu Jin(人迎脈口診), Wang Suk Wha(王叔和) recreated that the left is In Young(人迎), the right is Kie Gu(氣口). In future generations by Jin Mu Taek(陳無擇) who is the writer of Sam In Bang(三因方). In Young Mac Gu Jin(人迎脈口診) is a measure for disease which classify it's inside and outside cause. 5. Results for Chon Gu Mac Jin(寸口脈診) 1) What we say Mac Jin(脈診) of Chon Gu(寸口) two means are used in commn. First case, we simply say the area of Chon Gu(寸口), second case, we say Chon Kwan Chuk Jin(寸口尺診) reducingly. Chon Gu(寸口) is the area which is the radial artery of wrist joint. What we attemp diagnose by only Chon Gu Mac(寸口脈), it is clearly shoued in the method of Nan Kyoung, five Nan(難經五難). 2) Because Jin Mac(診脈) is made in only Chon Gu(寸口), that is the area in which is concentated Kyoung Kee(經氣). That is the birth of Jin Kee(眞氣) and Jin Kee(眞氣) is related with disease. We can diagnose disease by taking Chon Gu(寸口). 3) Chuk Jin(尺診) in Nae Kyoung(難經) have two things. One is Il Chuk(一尺), the other is Chon Kwan Chu(寸關尺). 4) Chuk Chon Jin(尺寸診) is the method which diagnose the difference of point and the condition of Mac(脈) by dividing a part of Chuk(尺) in the area of Chon Gu(寸口). In Chon Gu Jin(寸口診), by introducing the theory of Yin Yang(陰陽), the method of Chon Gu Jin(寸口診) is developed by chon Gu Jin(寸口診). 5) What Chuk Kwan Chon Jin(寸關尺診) is that area of the Chon Gu(寸口) are divided fot three point, we can diagnose. By consulting Sam Bu Gu Who Jin(三部九候診), developping of the method of acupunture, utilzing the theory of five element(五行) it is devised by concentrating way of thinking of the method mac Jin(脈診) exiting. 6) Chon Kwan Chuk Jin MaC(寸關尺診脈) begin from Nae Kyoung(內徑) exiting. After Nan Kyoung(內徑), spread out widely from Mac Kyoung(脈診) of Wang Suk wha(王叔和), the future medicins followed it. Yang Hyun Jo(楊玄操) and established Chon Kwan Chuk Jin(寸關尺診) which is used widely today. This right and left Chon Kwan Chuk Jin(寸關尺診), we call it method of Yuk Bu Jung Wee Jin Mac(六部定位診脈). 7) We can think the base which presume the arrangement of the viscera for Chon Kwan Chuk(寸關尺) of the right and the left. 8) The origin, which seperate the right and the left of Mac(脈), is showed at the treory of Ji Jin Yo Dae(至眞要大論) in So Mun(素問) which Chon Chuk(寸尺) seperate the right and the left. But the method of diagnosis in Nan Kyoung(難經) have no seperation the fight and the left. Otherwise this. there is clearly writtened the seperation for the right origin of the method of Yuk Bu Jung Wee Jin Mac(六部定位診脈) seek for Cang Gong(倉公). 9) Yang Hyun Jo(楊玄操) notice that the Chuk(尺) is mentioned for Sam Cho(三焦) in the method of Mac Kyuong(脈經), Sim Po Kyung(心包經) which put together with Sam Cho(三焦) allot on this, he had established the method of Yuk Bu Jung Wee Jin Mac(六部定位診脈). 10) On the method of Paen Jak Yin Yang Mac(扁鵲陰陽脈) in Mac Kyoung(脈經), equal article exist with the theory of Pyung In Kee Sang(平人氣象論) in So Mun(素問). When Wang Suk Wha(王叔和) write Mac Kyung(脈經), we can presume that the book of Mac(脈) which Paen Jak(扁鵲) had experienced the origin have exited besides So Mun(素問), Young Chu(靈樞). If so he must be make Chon Kwan Chuk Jin(寸關尺診) very fairly standard. So Nae Kyoung(內經), which must be fllowed the method of Paen Jak Mac(扁鵲脈), do the method diagnosis of Chon Kwan Chuk(寸關尺), diagnise of disease and treat.

• Food Science and Biotechnology
• /
• 제15권5호
• /
• pp.694-697
• /
• 2006

The initiation of immunoglobulin E (IgE)-mediated allergic reactions requires binding of IgE antibody to its high-affinity receptor. Human basophilic KU812F cells express $Fc{\varepsilon}RI$ on the cell surface and act as effector cells in the allergic response. In this study, we investigated the effects of Scutellaria radix extract on the expression of the $Fc{\varepsilon}RI$ in human KU812F cells. Flow cytometric analysis showed that S. radix extract treatment caused a concentration-dependent decrease in $Fc{\varepsilon}RI$ expression on the cell surface. Furthermore, the level of $Fc{\varepsilon}RI$ ${\alpha}$, ${\beta}$, and ${\gamma}$ chain mRNA in KU812F cells was examined by RT-PCR. S. radix extract reduced total cellular $Fc{\varepsilon}RI$ $\alpha$ and ${\gamma}$ chain mRNA expression in a concentration-dependent manner. $Fc{\varepsilon}RI$-mediated histamine release was reduced from $21.75{\pm}1.34\;ng/10^6$ cells in non-treated cells to $16.46{\pm}1.98\;ng/10^6$ cells in S. radix extract treated cells. These results suggested that S. radix extract has the potential to down-regulate of FcRI expression and to inactivate basophils.

• Journal of Advanced Marine Engineering and Technology
• /
• 제33권8호
• /
• pp.1220-1225
• /
• 2009

본 논문에서는 사냑 루프 필터와 광섬유 격자 기반의 파장가변 궤환 레이저를 제안하고 있다. 다중파장을 제공하기 위하여 복굴절을 이용한 사냑 루프를 적용하였고 0.33nm의 인접 채널간격을 가지도록 제작하였다. 광섬유 격자의 열 광학 효과에 의한 파장가변을 유도하기 위하여 니크롬선을 격자위에 감았다. 파장가변과 분리에 대한 특성을 측정하기 위하여 길이가 다른 니크롬선으로 두 종류의 광섬유 격자 소자를 제작하였으며, 열 저항으로 파장가변이 되게 하였다. 광섬유격자의 반사파장이 사냑루프 다중파장의 특정 파장과 일치하면 모드 잠김현상에 의해서 공진하고 발진하게 된다. 공진파장의 이동도는 1.75pm/mW이다. 이 시스템은 전력 시스템의 전력 변동을 모니터링하는 분야에 적용할 수 있다.

• 한국강구조학회 논문집
• /
• 제25권1호
• /
• pp.71-80
• /
• 2013

구조, 시공 및 내화측면에서 우수한 콘크리트충전강관(CFT)부재에 대해서 구성재료의 강도가 높은 경우 즉, 콘크리트 압축강도가 100MPa 이상이면서 강관의 항복강도가 650MPa 이상인 경우에 대한 내화성능 평가실험 및 해석적 연구는 미비한 실정이다. 본 연구에서는 이러한 부재에 대해서 내화성능 거동을 파악하고자 ${\Box}-400{\times}400{\times}15$, 부재길이 3,000mm인 실물대 중심재하 내화실험을 행하였다. 실험변수는 중심축력을 5,000kN과 2,500kN로 하였다. 실험결과, 고강도 콘크리트의 폭렬 및 콘크리트 내부 균열에 의하여 축하중이 클수록 조기에 내력을 상실하였다. 또한, 유한차분법과 변형률적합조건을 이용한 비정상온도해석 및 응력해석을 수행하였으며, 고강도 재료모델은 Eurocode모델을 이용하였다. 해석모델은 시간-축변위 관계를 합리적으로 예측할 수 있었다.

• 한국강구조학회 논문집
• /
• 제22권5호
• /
• pp.497-509
• /
• 2010

고강도강재 및 고강도콘크리트를 적용한 매입형 합성기둥의 편심압축시 거동특성을 연구하기 위하여, 항복강도 $f_{ys}$=913MPa 강재와 압축강도 $f_{ck}$97.7MPa 콘크리트를 사용하여 실험연구를 실시하였다. 주요변수는 횡철근의 종류, 항복강도, 배근간격, 그리고 편심하중거리이다. 거동특성을 분석하기 위하여 구속효과와 변형률적합조건을 적용한 비선형 단면해석을 실시하였으며, 현행 설계기준들의 적용성을 판단하기 위해 실험결과와 비교하였다. 횡구속효과에 의하여 콘크리트의 연성능력은 증가하였으며, 이에 따라 단면의 휨모멘트강도가 증가하였다. 비선형해석을 통해 합성단면의 축력-모멘트 상관관계를 예측한 결과는 실험결과와 잘 일치한 반면, 강재의 완전소성강도를 사용하는 Eurocode 4는 실험체의 성능을 과대평가하였으며, 콘크리트의 횡구속효과를 고려하지 않은 ACI 318은 과소평가하였다.