• Journal of Animal Science and Technology
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• v.63 no.6
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• pp.1247-1264
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• 2021

Anabolic steroids are frequently used to increase the growth rate of meat-producing animals. Exposure to an anabolic-androgenic steroid, nandrolone decanoate (ND), is associated with expressional reduction of testicular steroidogenic enzymes. However, the effect of withdrawal of ND exposure on the expression of these testicular molecules has not been thoroughly explored. The current research investigated expression changes of testicular steroidogenic enzymes in rats at several recovery periods (2, 6, and 12 weeks) after the stop of ND treatment with different doses (2 and 10 mg/kg body weight) for 12 weeks. Body and testis weights were recorded, and transcript levels of molecules were determined by quantitative real-time polymerase chain reaction (PCR). The immunohistochemistry was used to examine the changes of immuno-intensities of molecules. At 6 and 12 weeks of the recovery period, the 10 mg/kg ND-treated rats were lighter than other experimental groups. The interstitial compartment vanished by ND treatment filled up as the recovery period became longer. The expression of steroidogenic acute regulatory protein was returned to the control level at 12 weeks of the recovery period. Expression levels of cytochrome P450 side-chain cleavage and 17a-hydroxylase were increased in 2 mg/kg ND-treated group at 6 weeks of the recovery period, and transcript levels of these molecules in 2 and 10 mg/kg ND-treated groups at 12 weeks of the recovery period were significantly lower than the control. Expression levels of 3β-hydroxysteroid dehydrogenase (HSD) type I and 17β-HSD type 3 in 2 mg/kg ND-treated group were comparable with those of control at 12 weeks of the recovery period, but not in 10 mg/kg ND-treated group. Expression of cytochrome P450 aromatase (Cyp19) was reverted to the control level at 2 weeks of the recovery period. Except for Cyp19, there was a visible increase of immuno-staining intensity of other testicular steroidogenic enzymes in the Leydig cells as the recovery period progressed. This research has demonstrated that the cease of ND administration could restore the expression of testicular steroidogenic enzymes close to the normal level. Nevertheless, a relatively long recovery period, compared to the ND-exposure period would be required to retrieve normal expression levels of testicular steroidogenic enzymes.

• The Journal of the Korea Contents Association
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• v.19 no.7
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• pp.271-280
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• 2019

This study is to understand the effect of attachment and deviant peers on juvenile recidivism. The study analyzes a panel of Korean children, youths, and students who are in first year of middle school, and obtains the following results: First, juvenile recidivism increases by 1,5 times, from 180 people (10.2%) in third year of middle school to 278 people (15.7%) in third and last year of high school. 138 people stopped deviant behaviors (72.7%) and 42 people continued such acts (23.3%), showing that children come to stop deviant acts more than not. 236 people (85.6%) are found to have experienced engaging in deviant behaviors during third year of high school. Second, in terms of the effect of attachment and deviant peers in juvenile deviance, particularly attachment to parents, parental affection is found to have a negative (-) effect in third year of middle school, while parental supervision is found to have a negative (-) effect. In peer attachment, peer trust is found to havee positive (+) effects in third year of middle school and deviant peers are found to have positive (+) effects in third year of middle school and third year of high school. Third, in terms of the effect of youth attachment and deviant peers in juvenile recidivism, parental supervision has a negative (-) effect and deviant peers have a positive (+) effect. However, parental affection and peer attachment (friendship, trust) are not found to have an effect. Based on these results, the current study narrates suggestions for the practice of youth welfare.

• 한국노년학
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• v.38 no.3
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• pp.593-605
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• 2018

This study examines factors that influence older drivers to consider stopping driving in advance and whose advice plays an important role in the driving cessation decision. Data came from a nationally representative cross-sectional survey of community-dwelling older adults (N=2,076). About two thirds reported that they have considered stopping driving (62.8%) and they preferred to decide by themselves (66.0%). Logistic regression results showed that women and residents in urban areas were twice as likely to consider voluntary driving cessation compared to their counterparts. Each one year increase in age contributed to the probability that respondents would consider driving cessation by five percent. Those with greater difficulties in ADLs due to chronic illnesses were also more likely to consider driving cessation. No sociodemographic and health factors were related to whether older drivers preferred to decide by themselves or listen to others. These results imply that policies to promote self-motivation to stop driving among older drivers would be effective. Furthermore, they suggest it could be important to help older drivers plan their driving cessation in advance while still at a young-old age, and that more attention needs to be paid to men and those living in rural areas among older drivers.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.39 no.1
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• pp.93-103
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• 2019

In metropolitan cities have been reorganizing the routes of the city buses periodically due to changes in the spatial structure and traffic pattern and the lack of supply due to new developments. However, there is a lack of system to comprehensively evaluate the routes. Even if the evaluation index is not clearly defined, it may not be reasonable. Therefore, it is difficult to set the improvement direction when the bus route is reorganized. In this study, the existing evaluation indexes were reviewed and further investigation was conducted on the problematic indexes. In particular, the degree of curvature has been used as a very important index in the evaluation of the route system, but the existing curvature based curvature has a limitation in considering the traffic characteristics of individual users. For this purpose, a virtual city bus network was set up and the degree of curvature was calculated and compared based on the point - based curvature and stopping point based on individual user O/D. In this study, it is considered that more efficient and practical analysis and evaluation are possible in the evaluation of the city bus route system through the curvature considering the individual user O/D based on the stopping point. It is expected that it will be used at the time of reorganization of city bus route performed by individual local governments in the future.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.39 no.4
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• pp.505-512
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• 2019

This study is a basic study to prepare safety measures for the tram that is being introduced into Korea by reviewing accident analysis and accident compensation system of the tram. Analysis of accident characteristics of the tram was analyzed based on overseas data. Five years after the inauguration of the tram, accidents were halved and stabilized. Deaths accounted for 0.5 % of all casualties, most accidents were minor. Accidents with other mean transportation were high 84.1 %, among them accidents with passenger cars was found to be highest. The frequent points of accidents were analyzed as intersections and tram stop, and measures for pedestrian accidents were found to be important. Those are proposed safety measures through analyzing tram accident characteristics: Monitoring accidents which occur within five years of its inauguration, developing accident database input system, installation of fences for preventing people from jaywalking, operating traffic signal for preventing people from jaywalking through connection of pedestrian signals and tram signals, clamping down on illegal U-turn cars and jaywalking pedestrian, Also, those are proposed accident treatment measures based on general driver receptively and overseas data: property damage liability coverage (PI) of car insurance, comparative negligence when the accident occurs between car and tram.

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.420-427
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• 2018

The orthogonal pair of Saccharomyces cerevisiae tyrosyl-tRNA synthetase (Sc YRS) and a variant of E. coli initiator tRNA, fMam $tRNA_{CUA}$ which recognizes the amber stop codon is an effective tool for site-specific incorporation of unnatural amino acids into the protein in E. coli. To evolve the amber suppression activity of the orthogonal pair, we generated a mutant library of Sc YRS by randomizing two amino acids at 320 and 321 which involve recognition of the first base of anticodon in fMam $tRNA_{CUA}$. Two positive clones are selected from the library screening with chloramphenicol resistance mediated by amber suppression. They showed growth resistance against high concentration of chloramphenicol and their $IC_{50}$ values were approximately 1.7~2.3 fold higher than the wild type YRS. In vivo amber suppression assay reveals that mutant YRS-3 (mYRS-3) clone containing amino acid substitutions of P320A and D321A showed 6.5-fold higher activity of amber suppression compared with the wild type. In addition, in vitro aminoacylation kinetics of mYRS-3 also showed approximately 7-fold higher activity than the wild type, and the enhancement was mainly due to the increase of tRNA binding affinity. These results demonstrate that optimization of anticodon recognition by engineered aminoacyl tRNA synthetase improves the efficiency of unnatural amino acid incorporation in response to nonsense codon.

• Korean Journal of Materials Research
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• v.31 no.2
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• pp.75-83
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• 2021

To cope with automobile exhaust gas regulations, ISG (Idling Stop & Go) and charging control systems are applied to HEVs (Hybrid Electric Vehicle) for the purpose of improving fuel economy. These systems require quick charge/discharge performance at high current. To satisfy this characteristic, improvement of the positive electrode plate is studied to improve the charge/discharge process and performance of AGM(Absorbent Glass Mat) lead-acid batteries applied to ISG automotive systems. The bonding between grid and A.M (Active Material) can be improved by applying the Sand-Blasting method to provide roughness to the surface of the positive grid. When the Sand-Blasting method is applied with conditions of ball speed 1,000 rpm and conveyor speed 5 M/min, ideal bonding is achieved between grid and A.M. The positive plate of each condition is applied to the AGM LAB (Absorbent Glass Mat Lead Acid Battery); then, the performance and ISG life characteristics are tested by the vehicle battery test method. In CCA, which evaluates the starting performance at -18 ℃ and 30 ℃ with high current, the advanced AGM LAB improves about 25 %. At 0 ℃ CA (Charge Acceptance), the initial charging current of the advanced AGM LAB increases about 25 %. Improving the bonding between the grid and A.M. by roughening the grid surface improves the flow of current and lowers the resistance, which is considered to have a significant effect on the high current charging/discharging area. In a Standard of Battery Association of Japan (SBA) S0101 test, after 300 A discharge, the voltage of the advanced AGM LAB with the Sand-Blasting method grid was 0.059 V higher than that of untreated grid. As the cycle progresses, the gap widens to 0.13 V at the point of 10,800 cycles. As the bonding between grid and A.M. increases through the Sand Blasting method, the slope of the discharge voltage declines gradually as the cycle progresses, showing excellent battery life characteristics. It is believed that system will exhibit excellent characteristics in the vehicle environment of the ISG system, in which charge/discharge occurs over a short time.

• Journal of Life Science
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• v.30 no.12
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• pp.1118-1127
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• 2020

Alzheimer's disease causes progressive neuronal loss that leads to cognitive disturbances. It is not currently curable, and there is no way to stop its progression. However, since medical treatment for Alzheimer's disease is most effective in the early stages, early detection can provide the best chance for symptom management. Biomarkers for the diagnosis of Alzheimer's disease include amyloid β (Aβ) deposition, pathologic tau, and neurodegeneration. Aβ deposition and phosphorylated tau can be detected by cerebrospinal fluid (CSF) analysis or positron emission tomography (PET). However, CSF sampling is quite invasive, and PET analysis needs specialized and expensive equipment. During the last decades, blood-based biomarker analysis has been studied to develop fast and minimally invasive biomarker analysis method. And one of the remarkable findings is the involvement of platelets as a primary source of Aβ in plasma. Aβ can be transported across the blood - brain barrier, creating an equilibrium of Aβ levels between the brain and blood under normal condition. Interestingly, a number of clinical studies have unequivocally demonstrated that plasma Aβ42/Aβ40 ratios are reduced in mild cognitive impairment and Alzheimer's disease. Together, these recent findings may lead to the development of a fast and minimally invasive early diagnostic approach to Alzheimer's disease. In this review, we summarize recent advances in the biomarkers of Alzheimer's disease, especially the involvement of platelets as a source of peripheral Aβ production and its potential as a blood-based biomarker.

• 한국사회정책
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• v.20 no.2
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• pp.233-260
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• 2013

Despite various policies have been implemented to curb shadow education in Korea, it has continued to grow in recent two decades. This study investigates the expansion mechanism of shadow education focused on mobilization capabilities and market strategies of the suppliers. The success and failure of policy toward shadow education depends on how effectively it could block off the way by which the suppliers as the most important actors in politics of shadow education market mobilize consumers' anxieties. But shadow education policies have failed in two points. First, they have lacked honest intention to stop its proliferation. The Constitutional Court Decision Against Anti-Out-Of-School Classes Legislation of 2000 widened the windows of opportunity for the suppliers, and 5.31 educational reform of 1995 was neutral to their mobilization capabilities, though seemingly designed to control shadow education. This policy orientation, which reflected neoliberal Gesinnungsethik defective of Verantwortungsethik, stimulated shadow education to expand in that suppliers' mobilization capabilities were reinforced or remained intact. Second, shadow education suppliers have succeeded in mobilizing the desire and anxiety of potential consumers. To cope with government's policy including improving the qualities of public education, realignment of college entrance systems, and meeting the shadow education needs, they have developed various market strategies such as management of existing demands, creation of responsive demands, and squeezing out new demands. They have succeeded in nullifying policies by employing or mixing strategies with effect. Policy decisions in the future need to be made with reference to Verantwortungsethik, and be more cautious to socio-political contexts of Korea, to mobilization capabilities and market strategies of the suppliers in particular.

• Korean Journal of Optics and Photonics
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• v.31 no.6
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• pp.281-289
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• 2020

This paper reports a slim mobile lens design using a hybrid refractive/diffractive optical element. Conventionally a wide field of view (FOV) camera-lens design adopts a retrofocus type having a negative (-) lens at the forefront, so that it improves in imaging performance over the wide FOV, but with the sacrifice of longer total track length (TTL). However, we chose a telephoto type as a baseline design layout having a positive (+) lens at the forefront, to achieving slimness, based on the specification analysis of 23 reported optical designs. Following preliminary optimization of a baseline design and aberration analysis based on Zernike-polynomial decomposition, we applied a hybrid refractive/diffractive element to effectively reduce the residual chromatic spherical aberration. The optimized optical design consists of 6 optical elements, including one hybrid element. It results in a very slim telephoto ratio of 1.7, having an f-number of 2.0, FOV of 90°, effective focal length of 2.23 mm, and TTL of 3.7 mm. Compared to a comparable conventional lens design with no hybrid elements, the hybrid design improved the value of the modulation transfer function (MTF) at a spatial frequency of 180 cycles/mm from 63% to 71-73% at zero field (0 F), and about 2-3% at 0.5, 0.7, and 0.9 fields. It was also found that a design with a hybrid lens with only two diffraction zones at the stop achieved the same performance improvement.