• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.4
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• pp.345-352
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• 1989

The immunochemical property of lectin in soybean and the presence of lectin in seeds of six soybean cultivars were investigated by immunochemical methods. All seeds of six soybean cultivars formed one precipitin line against the soybean lectin antibody and showed immunochemically homologous precipitin pattern among the cultivars in immunodouble diffusion test. Four antigenic components in soybean lectin were detected by the crossed imuno-electrophoresis of a soybean seed antigen against the soybean lectin antibody. Cultivar, Jangyeop-kong contained the highest amount of lectin and the next were Kwangkyo, Hwangkeumkong, Baegunkong, and Jangbaegkong or Paldal-kong in order of lectin content. The lectin purified from the seeds of six soybean cultivars agglutinated rabbit erythrocytes, but did not agglutinate the fungal spores which were Fusarium sp., Alternaria sp., Cuvalaria sp., and Colletotrichum sp. isolated from the infected seeds or leaves of soybean.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.4
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• pp.265-267
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• 2005

Study on effectiveness of Bradyrhizobium japonicum on soybean [Glycine max (L.) Merr.], local cultivar 'Sathiya' were carried out in the garden soil of Trib-huvan University. Different parameters like nodulation, chlorophyll content in fresh leaves and growth of plant in inoculated and uninoculated plant was studied. Pot experiment was conducted in the green house to evaluate the effectiveness of B. japonicum on soybean. It was observed that B. japonicum inoculation increased the number of nodules, shoot length of plant and total chlorophyll content in fresh leaves of soybean plant. However, root length was decreased in all inoculated plants.

• Korean Journal Plant Pathology
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• v.14 no.3
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• pp.247-252
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• 1998

A series of greenhouse test was conducted to evaluate infection of Calonectria ilicicola on soybean plants of different ages at time of inoculation. Lesion length and number of perithecia were determined on cultivars Braxton, Deltapine 726, and Riverside 699 that were 10∼40 days old and 4-10 days old at time of inoculation. Quadratic and linear relationships were described between plant age at inoculation and lesion length or perithecia production in greenhouse studies. Soybean seedlings exhibited low susceptibility to C. ilicicola regardless of cultivar susceptibility. On 8- or 1-0-days-old Braxton, lesion lenght and perithecia numbers were reduced. Lesion lengths were longest on plants 30 days old whereas perithecia production was greatest on plants 20∼30 days old at time of inoculation. Differences in lesion length and perithecia production that were observed on young plants (4∼10 days old) were similar to relative levels of susceptibility in soybean cultivars in greenhouse and field tests, suggesting that reaction to C. ilicicola in soybean cultivars may be determined early in plant development.

• The Plant Pathology Journal
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• v.37 no.6
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• pp.652-661
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• 2021

Xanthomonas citri pv. glycines (Xcg) is a major pathogen of soybean (Glycine max) in South Korea, despite the availability of soybean varieties with some resistance. We conducted a nationwide survey of the incidence and severity of bacterial pustule caused by Xcg. The percentage of infected fields was 7% to 17% between 2015 and 2017. We characterized the diversity of a nationwide collection of 106 Xcg isolates based on avrBs3 banding patterns. The isolates fell into 11 groups, each represented by a type strain; only two of these were similar to isolates collected from 1999 to 2002. The diversity of Xcg strains increased and the dominant strains changed between 1999 and 2017, with three new type strains comprising 44% of the isolates examined in 2012 to 2017. Pathogenicity tests did not show evidence for a shift in the races or aggressiveness of Xcg strains. Korean soybean cultivars, including the widely-grown Daewon cultivar, were susceptible to the 11 new type strains. The cultivar CNS, which carries the rxp resistance gene, was susceptible to most type strains, including two representing 83% of the Korean Xcg strains. In contrast, Williams 82, which also carries rxp, showed resistance to at least five type strains. Collectively, these results suggest that Williams 82 has resistance loci in addition to rxp. The widespread distribution of Xcg, the high virulence of the current endemic strains, and the low resistance of most Korean soybean cultivars collectively favor widespread disease in Korea in years that are favorable to pustule development.

• Molecules and Cells
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• v.25 no.2
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• pp.247-252
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• 2008

There are several branch points in the flavonoid synthesis pathway starting from chalcone. Among them, the hydroxylation of flavanone is a key step leading to flavonol and anthocyanin. The flavanone 3-${\beta}$-hydroxylase (GmF3H) gene was cloned from soybean (Glycine max cultivar Sinpaldal) and shown to convert eriodictyol and naringenin into taxifolin and dihydrokaempferol, respectively. The major flavonoids in this soybean cultivar were found by LC-MS/MS to be kamepferol O-triglycosides and O-diglycosides. Expression of GmF3H and flavonol synthase (GmFLS) was induced by ultraviolet-B (UV-B) irradiation and their expression stimulated accumulation of kaempferol glycones. Thus, GmF3H and GmFLS appear to be key enzymes in the biosynthesis of the UV-protectant, kaempferol.

• Journal of Biosystems Engineering
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• v.37 no.6
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• pp.398-403
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• 2012

Purpose: Green soybeans (edamame) are now an economically important and popular food product in Japan. In order to shorten breeding time and to decide an optimal harvest time, we have been developing a dedicated NIRT spectrometer since 2004 for the determination of constituent content such as sucrose and free amino acids, which are two major contributors to the eating quality, in a single pod green soybean. Methods: The obtained models showed that the developed NIRT instrument had reasonable accuracy for the determination of these two components. Then we carried out the investigation into the change in two components during a few days storage using these models with changing time, variety/cultivar, packaging and temperature. Results: The result showed that the most affecting factor on decreasing both sucrose content and free amino acids was variety/cultivar. The time, packaging and temperature also affected significantly in most cases.

• Korean Journal of Organic Agriculture
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• v.22 no.4
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• pp.773-787
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• 2014

Bacterial pustule of soybean caused by Xanthomonas axonopodis pv. glycines is one of the most prevalent bacterial diseases in many areas where soybeans are grown. This study was carried out to evaluate the effect of cultivars, sowing date and cropping system on the suppression of soybean bacterial pustule in the field. One hundred soybean cultivars were screened for disease resistance against bacterial pustule in naturally infested field. Among them, fourteen cultivars including 'Pureun' were found to be high resistant. And thirty cultivars showed to be moderate resistant(less than 3% of diseased leaf area). When Soybean cultivar 'Taekwang' were sown in four different dates, May 25, June 5, June 15, and June 25, at 10 day-interval in Milyang, the diseased leaf area of bacterial pustule was 23.3%-25.7%, 14.7%-18.0%, 10.7%-12.8%, and 1.0%-2.7%, respectively. The lowest percentage of diseased leaf area was recorded in the plots sown on June 25, whereas the highest percentage of diseased leaf area was recorded in the plot sown on May 25. As sowing time was delayed, incidence of soybean bacterial pustule found to be comparatively reduced. From December in 2006 to June in 2007, we surveyed the pathogen population of soybean bacterial pustule in five cropping upland soils where soybean was cultivated. The survey result showed the bacterial pustule pathogens were detected from the all cropping soils. The pathogen populations of soybean bacterial pustule in soybean-barley and soybean-garlic cropping soil were significantly lower than that of the other cropping soils. In addition, the incidence of soybean bacterial pustule was decreased under the two cropping systems.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.7
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• pp.658-668
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• 2006

The objective of this study was to develop a technique for the cultivar discrimination using SSR markers in soybean. A total of 91 soybean cultivars developed from 1913 to 2002 in Korea were evaluated by five polymorphic SSR markers (Sat_043, Sat_036, Sat_022, Sat_088 and Satt045). Five SSR markers generated a total of 64 alleles and the number of alleles for each SSR marker ranged from 10 to 15 with average of 12.8. Polymorphic information contents (PIC) by five markers of 91 cultivars were ranged from 0.790 to 0.905 with average of 0.857. A total of 82 cultivars (90%) among 91 soybean cultivars could be individually discriminated by combination of five SSR markers through five step analysis. A cultivar, Buseok, by Sat_043 at the first step, 34 cultivars including Hojangkong by Sat_036 at the second step, 29 cultivars including Dankyeongkong by Sat_022 at the third step, 12 cultivars including Sinpaldalkong 2 by Sat_088 at the fourth step, and 6 cultivars including Saebyeolkong by Satt045 at the fifth step were discriminated. Soybean cultivars which were not discriminated by SSR markers could be discriminated by morphological characteristics.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.4
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• pp.227-231
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• 2000

Small seed size is one of the major traits of soybean cultivars for sprouts with regard to high sprout yield. This study was conducted to identify quantitative trait loci (QTL) for seed size and weight in a set of F 6 seeds of 89 lines derived from a cross between 'Pureunkong', a soybean cultivar developed for sprouts and 'Jinpumkong 2', a soybean cultivar with no beany taste in seed due to the lack of lipoxygenases. The genetic map of 25 linkage groups with a total of 98 markers including RFLP, RAPD, SSR and classical markers was constructed from this F/sbu 5/-derived population and was used for QTL analysis. 'Pureunkong' was significantly smaller (P＜0.01) than 'Jinpumkong 2' in seed size and seed weight. Genetic variation was detected and transgressive segregation was common in the population for these traits. Seven DNA markers including opT14-1600 in LG A2, opF02-400 in LG B2, Satt100, opC09-700, opG04-730 and opQll-650 in LG C2, and opY07-1100 ＆ 1000 in LG(unknown) were significantly associated and accounted for 4.7 to 10.9% and 5.1 to 10.1 % of the phenotypic variation in seed size and seed weight, respectively. 'Pureunkong' alleles increased seed size and seed weight at the all four significant marker loci on the LG C2. These marker loci in LG C2 were closely linked and were presumed to be a single QTL. Overall, at least three independent QTLs from 3 linkage groups (A2, B2, and C2) were putatively involved in the control of seed size and seed weight.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.4
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• pp.365-372
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• 2012

Native of soybean aphid (Aphis glycines Matsumura) is an Asia and aphid is one of the dangerous pests in soybean [Glycine max (L.) Merr.]. High density aphid populations can reduce crop production by causing severe damage. The objective of this study was evaluation of resistance to the soybean aphid in soybean cultivars and germplasms. A total of fifty five soybean cultivars or germplasms, including two susceptible and two resistant check varieties, were infested to evaluate their resistance in the field cage and greenhouse test by aphid colonies which derived from wild collected one soybean aphid biotype in Korea. The average number of reproduced soybean aphid was evaluated with 62.7 aphids in the resistant check variety PI 567598B and also estimated with 1,807 aphids for susceptible check variety Williams 82. In soybean varieties and germplasms, the average reproduced soybean aphid populations ranged from the lowest 497 aphids for Junjeori to the highest 3,862 aphids for Mansu. About seventy six percent of soybean cultivars and germplasms were shown high density soybean aphid populations when compared with another susceptible check variety PI 567543C in the field cage test. From the greenhouse test to evaluate aphid index, 87.3% of soybean cultivars or germplasms presented aphid index with 9.0. No soybean cultivars and germplasms were observed with soybean resistant phenotype when regarded a aphid resistant level as less than 10% aphid reproductions compared with susceptible check Williams 82. Although no Korean soybean cultivars were identified with resistant trait to the soybean aphid, we found one great resistant genetic resource PI 567598B in this study. This result will be helpful to further study for providing useful genetic information for soybean researchers.