• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.39-39
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• 2002

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• 농업과학연구
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• 제45권4호
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• pp.687-694
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• 2018

This study was conducted to investigate the laying performance of six strains of Korean domestic chickens (KDC 1A, 2A, 3A, 1B, 2B and 3B). A total of 288 20-week-old KDC hens were allocated in a completely randomized design to give 12 replicates per each strain (4 chickens per pen). The chickens were fed commercial diets (i.e., week 20 - 32, crude protein [CP] 18.0% and metabolizable energy [ME] 2,850 kcal/kg; week 32 - 40, CP 17.0% and ME 2,800 kcal/kg) ad libitum together with fresh water during the 20-week experiment. Body weight, age of sexual maturity, egg weight, hen-day egg production (HDP) and feed conversion ratio (FCR) were measured during the experiment period (week 20 - 40). Our results show that strain 3B had an improved (p < 0.05) HDP and FCR compared with the other strains on week 24 - 28. However, strain 3A had an improved (p < 0.05) HDP and FCR compared to the other strains on week 28 - 40. Similarly, strain 3A had a higher egg weight (p < 0.05) compared to the other strains on week 28, 32 and 40. In conclusion, the higher laying performance of strain 3A indicates its potential in the development of a KDC strain with improved commercial laying performance.

• 대한수의학회지
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• 제28권1호
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• pp.115-118
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• 1988

Mating experiments were performed to, elucidate the distribution of perfect state of Microsporum gypseum complex originated from animals and soils in Korea. A total of 30 strains of M. gypseum complex, composed of 16 from animals and 14 from soils, ere mated with the tester strains of Nannizzia incurvata, N. gypsea and N. fulva. Among 30 strains of M. gypseum complex examined, 16 strains(53.3%) were N. incurvata, 13 strains(43.3%) N. gypsea and 1 strain (3.3%) N. fulva. Among 13 strains of dog isolates, 8 strains(61.5%) were N. incurvata and 5 strains(38.5%) N. gypsea. And each strain isolated from Korean native goat, monkey and albino rat was N. gypsea. Among 14 strains of soil isolates, 8 strains(57.2%) were N. incurvata, 5 strains (35.7%) N. gypsea and 1 strain(7.2%) N. fulva. And -mating type was more frequently observed than +mating type in the strains of N. incurvata and N. gypsea. By this study, N. fulva was isolated the first time in Korea.

• Fisheries and Aquatic Sciences
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• 제6권1호
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• pp.20-26
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• 2003

A bacterial strain was isolated from seawater to screen for phytase activities. A colony had the highest activity and was identified as an Escherichia coli strain. Using primers derived from E. coli acid phosphatase appA sequence, we cloned a 1,495 bp DNA fragment connected with the pGEM-T vector. It was over-expressed under lac promoter combined with its native promoter in E. coli $DH5\alpha$. The expression of the phytase gene occurred during late exponential growth and the intracellular phytase production was 16.9 units/ml. The yield of recombinant phytate was 412-fold higher than that of wild type E. coli K13.

• 한국식물병리학회지
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• 제11권1호
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• pp.53-60
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• 1995

Inoculation with the compatible strain Ds 1 of Xanthomonas campestris pv. vesicatoria caused brownish ad water-soaked lesions, but incompatible strain Bv5-4a produced hypersensitive symptoms with local necrosis on tomato (cv. Kwangyang) leaves. Bacterial populations of the compatible strains Ds 1 propagated more greatly than the incompatible strain Bv5-4a at the frist onset, but no differences were observed 5 days after inoculation. The bacterial infection induced the synthesis and accumulation of soluble proteins in tomato leaves, especially in the incompatible interaction. Native-polyacrylamide gel electrophoresis distinguished the soluble proteins in the tomato leaves infected by the compatible or incompatible strains. A protein of low molecular weight occurred only in the incompatible interaction. Some pathogenesis-related (PR) proteins, especially the 15, 18, 23, 26 and 54 kDa proteins, were detected only in the infected tomato leaves. In the two-dimensional electrophoresis, some proteins with different molecular weights (Mr. 21∼29 kDa) and the pI 8∼9 appeared more distinctly only in the incompatible interaction. These data suggest that the de novo synthesis of some PR proteins in tomato may be significant in defense against X. c. pv. vesicatoria.

• 대한수의학회지
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• 제43권4호
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• pp.667-675
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• 2003

The safety of Brucella abortus strain RB51(SRB51) was investigated in dairy cows and Korean native cattle of 4~7th month of gestation. From experimentally inoculated cattle, 18 of 25 (72.0%) dairy cows, and 3 of 10 (30.0%) Korean native cattle were aborted or delivered premature fetus. There were no significant differences in the incidence of abortion depending on the inoculation route (intramuscular and subcutaneous) and dosages ($1{\times}10^9$, $2.8{\times}10^9$, and $4.0{\times}10^9$ CFU). The antibodies to the SRB51 were measured by a dot blot enzyme-linked immunosorbent assay. The highest titers to SRB51 were detected between 5~7 weeks after inoculation and the specitic antibody could be detected up to 28 weeks after inoculation. The SRB51 was isolated from amnio-allantoic fluid, bronchial lymph node, mammary gland, and supramammary lymph node in 5 of 25 dairy cows during 4 weeks after either abortion or delivery. Although SRB51 was isolated from 4 of 24 aborted fetus or normally delivered calves at parturition time, it was not isolated during 4 weeks afterward. Eleven of twentyfive dairy cows showed the endometritis and/or necrosis until 6 weeks after delivery, no lesions were seen at 8 weeks after delivery and uterus from control dairy cows. The results of present study revealed that SRB51 might induce the clinical signs of brucellosis in the pregnant cattle at 4~7th month of gestation.

• 농업과학연구
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• 제45권3호
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• pp.485-492
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• 2018

Two experiments were conducted to investigate the effect of feeding a diet supplemented with swine hair protein (SHP) on the nutrient digestibility of Korean native chickens and their egg production as well as on egg quality. In experiment 1, twenty roosters of the Hanhyup-3 strain were assigned to an individual cage to give 10 replicates per treatment (i.e., 0 and 10% of SHP), and the nutrient digestibility in response to the SHP supplementation was measured. In experiment 2, fifty-four layers of the Hy-Line strain were assigned to an individual cage to give 27 replicates per treatment (i.e., 0 and 10% of SHP), and egg production and egg quality were measured. In experiment 1, no significant difference (p > 0.05) was found in the digestibility of crude protein, crude fat, nitrogen-free extract (NFE), and crude fiber in response to the diet supplemented with SHP. In experiment 2, although no significant difference (p > 0.05) was found with the dietary treatments, the 10% SHP supplemented diet decreased (p < 0.05) the feed intake of laying hens. However, the SHP supplemented diet did not affect (p > 0.05) the laying rate, egg weight and feed conversion ratio of those fed the diet. Eggshell thickness and yolk color decreased (p < 0.05); however, eggshell strength, eggshell color, albumen height and Haugh units increased (p < 0.05) by feeding layers a diet supplemented with SHP. In conclusion, the results suggest that 10% SHP supplemented in a poultry diet could be a useful protein source.

• 한국초지조사료학회지
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• 제18권2호
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• pp.79-88
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• 1998

본 시험은 최적 Silage 옥수수 품종을 선발하는데 필요한 기초자료를 얻기 위하여 국내산 옥수수 4품종 (수원 19호, 광안옥, 횡성옥, 수원옥)과 외국 도입 Silage용 품종으로 미국계통 7품종 (P 3144 W, P 3352, P 3156, DK 501, DK 729, DK 713, DK 689)과 프랑스 Limagrain계통 6품종(H 545.64, H 645.12, H 643.99, He 7466, H 644.18, H ALISEO) 총 17품종의 생육특성, 수량 및 생장해석을 비교 분석하고자 실시 하였으며 실험설계는 품종을 란괴법 3반부으로 공주대학교 산업과학대학 실험포장에서 1997년 5월부터 8월까지 시행하였다. 그 결과를 요약하면 다음과 같다. 1. 생육특성으로 출현솔은 H 643.99 품종이 97.0% 로 가장 높았고, 흑조강축병 권병솔은 HC 7466 품종이 1.6 %로 가장 낮았다. 초장과 정장은 Pioneer 계통이 커서 초장은 P 3144w 품종이 339 cm로 가장 컸으며, 정장은 P 3156 품종이 261 cm로 가장 컸다. 우리나라 품종으로는 광안옥이 비교적 초장파 정장이 커서 각각 306 cm와 235 cm로 나타났다. 경직경은 DK 689 품종이 3.7 cm로 가장 굵었다. 2. 엽수와 엽면적은 광안옥이 각각 16.0매 와 $5,180\;{\textrm{m}^2}/10a$로 가장 높았으며, 암이삭 비율에 있어서는 H 645.12와 H 545.64 품종들이 각각 49.5와 49.4 %로 높게 나타났다. 3. 생초수량은 생육기에 따라 차이가 있어 출사전 15일에는 수원 19호가 출사기에는 P 3352 품종이, 출사 후 35일에는 광안옥이 가장 높아 우리나라 품종들이 도입 품종들보다 생초수량이 떨어지지 않았다. 4. 건물수량은 출사 후 중가 폭이 컸으며, 품종간의 건물수량은 생육기에 따라 약간의 차이는 있으나 P 3352, P 3156, 광안옥, DK 713의 품종들이 높았고, DK 501과 HC 7466 품종들이 낮았다. 출사 후 35일 경에는 품종간에도 유의성이 커서 우리나라 품종인 광안옥과 건물솔이 높은(29.6~30.2%) Pioneer 계통의 품종들이 수량이 많았으며 , Limagrain 계통의 Hybrid 품종들이 대체로 건물수량이 낮았다. 5. LAR은 HC 7466 품종이 $6.53\;{\textrm{cm}^2}/g$으로 제일 높았으며, 가장 낮은 품종은 H 545.12로 $3.30\;{\textrm{cm}^2}/g$이었다. LAI는 P 3144w가 제일 컸으며, HC 7466 품종의 3.15를 비롯하여 Limagrain 계통 품종들이 낮게 나타났다. 6. RGR은 품종간에 통계적 유의성은 없었으나 DK 501과 HC 7466 품종은 타 품종에 비해 떨어지는 경향을 보였으며, 국내품종과 미국계통 품종들은 CGR에 별 차이가 없으나, 프랑스의 Limagrain 계통 품종들 간에는 CGR의 변이가 크게 나타났다. 이상의 결과로 볼 때 출현솔 및 흑조강축병 이병솔은 Limagrain 계통 품종들이 양호하게 나타났으며, 사일리지 옥수수용 품종의 조건인 건물수량, 건물솔 및 암이삭비율 등은 P 3156, P 3352, P 3144w, DK 713, HC 7466 품종들이 우수한 것으로 나타났다. 우리나라 품종인 수원 19호와 광안옥도 생초수량과 건물수량은 타 도입 품종보다 떨어 지 지 않으나 건물솔이 낮게 나타났다.

• Journal of Microbiology and Biotechnology
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• 제24권4호
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• pp.475-482
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• 2014

The metabolic pathway of eugenol degradation by thermophilic Geobacillus sp. AY 946034 strain was analyzed based on the lack of data about eugenol degradation by thermophiles. TLC, GC-MS, and biotransformation with resting cells showed that eugenol was oxidized through coniferyl alcohol, and ferulic and vanillic acids to protocatechuic acid before the aromatic ring was cleaved. The cell-free extract of Geobacillus sp. AY 946034 strain grown on eugenol showed a high activity of eugenol hydroxylase, feruloyl-CoA synthetase, vanillate-O-demethylase, and protocatechuate 3,4-dioxygenase. The key enzyme, protocatechuate 3,4-dioxygenase, which plays a crucial role in the degradation of various aromatic compounds, was purified 135-fold to homogeneity with a 34% overall recovery from Geobacillus sp. AY 946034. The relative molecular mass of the native enzyme was about $450{\pm}10$ kDa and was composed of the non-identical subunits. The pH and temperature optima for enzyme activity were 8 and $60^{\circ}C$, respectively. The half-life of protocatechuate 3,4-dioxygenase at the optimum temperature was 50 min.

• Fisheries and Aquatic Sciences
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• 제13권1호
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• pp.49-55
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• 2010

A bacterial strain with phytase and glucose-1-phosphatase activity was isolated from seawater. The colony was identified as an Enterobacter cloacae strain and named E. cloacae B11. A gene, agpEnB11, coding for an intracellular acid glucose phosphatase was cloned from the strain and sequenced. It comprised 1,242 nucleotides and encoded a polypeptide of 413 amino acids. Recombinant glucose-1-phosphatase (AgpEn) was overexpressed in Escherichia coli and purified using Ni-NTA column under native conditions. Purified protein displayed a single band of 47 kDa on SDS-PAGE. AgpEn hydrolyzed a wide variety of phosphorylated compounds, with high activity for glucose-1-phosphate and glucose-6-phosphate. Optimum pH and temperature for enzyme activity were pH 5.0 and $50^{\circ}C$, respectively. Enzyme activity was stimulated by $Ca^{2+}$ and $Co^{2+}$, and inhibited by $Cu^{2+}$.