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The Safety of Brucella abortus strain RB51 in pregnant cattle  

Kim, Jong-man (National Veterinary Research and Quarantine Service)
Woo, Sung-ryong (National Veterinary Research and Quarantine Service)
Lee, Ji-youn (National Veterinary Research and Quarantine Service)
Jung, Suk-chan (National Veterinary Research and Quarantine Service)
Jean, Young-hwa (National Veterinary Research and Quarantine Service)
Kim, Jae-hoon (National Veterinary Research and Quarantine Service)
Kweon, Chang-hee (National Veterinary Research and Quarantine Service)
Yoon, Young-dhuk (National Veterinary Research and Quarantine Service)
Yoo, Han-sang (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
Publication Information
Korean Journal of Veterinary Research / v.43, no.4, 2003 , pp. 667-675 More about this Journal
Abstract
The safety of Brucella abortus strain RB51(SRB51) was investigated in dairy cows and Korean native cattle of 4~7th month of gestation. From experimentally inoculated cattle, 18 of 25 (72.0%) dairy cows, and 3 of 10 (30.0%) Korean native cattle were aborted or delivered premature fetus. There were no significant differences in the incidence of abortion depending on the inoculation route (intramuscular and subcutaneous) and dosages ($1{\times}10^9$, $2.8{\times}10^9$, and $4.0{\times}10^9$ CFU). The antibodies to the SRB51 were measured by a dot blot enzyme-linked immunosorbent assay. The highest titers to SRB51 were detected between 5~7 weeks after inoculation and the specitic antibody could be detected up to 28 weeks after inoculation. The SRB51 was isolated from amnio-allantoic fluid, bronchial lymph node, mammary gland, and supramammary lymph node in 5 of 25 dairy cows during 4 weeks after either abortion or delivery. Although SRB51 was isolated from 4 of 24 aborted fetus or normally delivered calves at parturition time, it was not isolated during 4 weeks afterward. Eleven of twentyfive dairy cows showed the endometritis and/or necrosis until 6 weeks after delivery, no lesions were seen at 8 weeks after delivery and uterus from control dairy cows. The results of present study revealed that SRB51 might induce the clinical signs of brucellosis in the pregnant cattle at 4~7th month of gestation.
Keywords
Brucella abortus; SRB51; safety; pregnant cattle; Dot-ELISA;
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